+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4c3i | ||||||
---|---|---|---|---|---|---|---|
タイトル | Structure of 14-subunit RNA polymerase I at 3.0 A resolution, crystal form C2-100 | ||||||
要素 |
| ||||||
キーワード | TRANSFERASE (転移酵素) | ||||||
機能・相同性 | 機能・相同性情報 RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / regulation of cell size / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping ...RNA polymerase III activity / RNA polymerase I preinitiation complex assembly / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / : / RNA Polymerase III Transcription Initiation From Type 2 Promoter / regulation of cell size / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / Formation of TC-NER Pre-Incision Complex / RNA polymerase II transcribes snRNA genes / RNA Polymerase I Promoter Escape / TP53 Regulates Transcription of DNA Repair Genes / Estrogen-dependent gene expression / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA-templated transcription / termination of RNA polymerase III transcription / RNA Polymerase II Pre-transcription Events / Dual incision in TC-NER / RNA polymerase I activity / termination of RNA polymerase I transcription / transcription initiation at RNA polymerase III promoter / tRNA transcription by RNA polymerase III / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / transcription initiation at RNA polymerase I promoter / transcription elongation by RNA polymerase I / RNA polymerase I complex / transcription by RNA polymerase I / RNA polymerase III complex / transcription by RNA polymerase III / RNA polymerase II, core complex / promoter-specific chromatin binding / transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / ribonucleoside binding / ポリメラーゼ / ペルオキシソーム / リボソーム生合成 / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / nucleic acid binding / protein dimerization activity / 核小体 / negative regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / 核質 / metal ion binding / 細胞核 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | SACCHAROMYCES CEREVISIAE (パン酵母) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 3 Å | ||||||
データ登録者 | Fernandez-Tornero, C. / Moreno-Morcillo, M. / Rashid, U.J. / Taylor, N.M.I. / Ruiz, F.M. / Gruene, T. / Legrand, P. / Steuerwald, U. / Muller, C.W. | ||||||
引用 | ジャーナル: Nature / 年: 2013 タイトル: Crystal Structure of the 14-Subunit RNA Polymerase I 著者: Fernandez-Tornero, C. / Moreno-Morcillo, M. / Rashid, U.J. / Taylor, N.M.I. / Ruiz, F.M. / Gruene, T. / Legrand, P. / Steuerwald, U. / Muller, C.W. | ||||||
履歴 |
| ||||||
Remark 700 | SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 7-STRANDED BARREL THIS IS REPRESENTED BY A 8-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "AI" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "GA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 5-STRANDED BARREL THIS IS REPRESENTED BY A 6-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4c3i.cif.gz | 1.7 MB | 表示 | PDBx/mmCIF形式 |
---|---|---|---|---|
PDB形式 | pdb4c3i.ent.gz | 1.4 MB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4c3i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/c3/4c3i ftp://data.pdbj.org/pub/pdb/validation_reports/c3/4c3i | HTTPS FTP |
---|
-関連構造データ
-リンク
-集合体
登録構造単位 |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
単位格子 |
|
-要素
-DNA-DIRECTED RNA POLYMERASE I SUBUNIT ... , 7種, 7分子 ABDGIMN
#1: タンパク質 | 分子量: 186676.969 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P10964, ポリメラーゼ |
---|---|
#2: タンパク質 | 分子量: 135910.328 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P22138, ポリメラーゼ |
#4: タンパク質 | 分子量: 14585.103 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P50106 |
#7: タンパク質 | 分子量: 36264.852 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P46669 |
#9: タンパク質 | 分子量: 13676.566 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P32529 |
#13: タンパク質 | 分子量: 46721.707 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: Q01080 |
#14: タンパク質 | 分子量: 26933.518 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P47006 |
-DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT ... , 2種, 2分子 CK
#3: タンパク質 | 分子量: 37732.613 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P07703 |
---|---|
#11: タンパク質 | 分子量: 16167.860 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P28000 |
-DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC ... , 5種, 5分子 EFHJL
#5: タンパク質 | 分子量: 25117.094 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P20434 |
---|---|
#6: タンパク質 | 分子量: 17931.834 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P20435 |
#8: タンパク質 | 分子量: 16525.363 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P20436 |
#10: タンパク質 | 分子量: 8290.732 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P22139 |
#12: タンパク質 | 分子量: 7729.969 Da / 分子数: 1 / 由来タイプ: 天然 詳細: STRAIN SC1613, ALIAS YPR110C, PROVIDED BY CELLZOME AG IN HEIDELBERG, GERMANY, MODIFIED TO EXPRESS ENDOGENOUS AC40 FUSED WITH A C-TERMINAL TAP-TAG. 由来: (天然) SACCHAROMYCES CEREVISIAE (パン酵母) / 株: SC1613 / 参照: UniProt: P40422 |
-非ポリマー , 4種, 12分子
#15: 化合物 | #16: 化合物 | ChemComp-ZN / #17: 化合物 | ChemComp-MG / | #18: 化合物 | ChemComp-MPD / ( | |
---|
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
---|
-試料調製
結晶 | マシュー密度: 2.88 Å3/Da / 溶媒含有率: 60 % / 解説: NONE |
---|---|
結晶化 | 詳細: 10% MPD, 0.1M TRIS HCL PH 7.4 |
-データ収集
回折 | 平均測定温度: 100 K |
---|---|
放射光源 | 由来: シンクロトロン / サイト: PETRA III, EMBL c/o DESY / ビームライン: P14 (MX2) / 波長: 0.97626 |
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2013年3月18日 |
放射 | モノクロメーター: SI 111 / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.97626 Å / 相対比: 1 |
反射 | 解像度: 3→84 Å / Num. obs: 132738 / % possible obs: 99.2 % / Observed criterion σ(I): -3 / 冗長度: 7.1 % / Biso Wilson estimate: 89.25 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 12.33 |
反射 シェル | 解像度: 3→3.18 Å / 冗長度: 7.1 % / Mean I/σ(I) obs: 0.83 / % possible all: 98.3 |
-解析
ソフトウェア |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
精密化 | 構造決定の手法: 分子置換 / 解像度: 3→84 Å / Cor.coef. Fo:Fc: 0.9413 / Cor.coef. Fo:Fc free: 0.9273 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 1.998 / SU Rfree Blow DPI: 0.336 詳細: IDEAL-DIST CONTACT TERM CONTACT SETUP. RESIDUE TYPES WITHOUT CCP4 ATOM TYPE IN LIBRARY=MG ZN. NUMBER OF ATOMS WITH PROPER CCP4 ATOM TYPE=34244. NUMBER WITH APPROX DEFAULT CCP4 ATOM TYPE=0. ...詳細: IDEAL-DIST CONTACT TERM CONTACT SETUP. RESIDUE TYPES WITHOUT CCP4 ATOM TYPE IN LIBRARY=MG ZN. NUMBER OF ATOMS WITH PROPER CCP4 ATOM TYPE=34244. NUMBER WITH APPROX DEFAULT CCP4 ATOM TYPE=0. NUMBER TREATED BY BAD NON-BONDED CONTACTS=8.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 120.5 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.656 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 3→84 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS精密化 シェル | 解像度: 3→3.08 Å / Total num. of bins used: 20
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 TLS | 手法: refined / Origin x: -63.2849 Å / Origin y: -67.508 Å / Origin z: 59.4621 Å
|