登録情報 データベース : EMDB / ID : EMD-17227 ダウンロードとリンクタイトル Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome. マップデータ 詳細 試料複合体 : A complex of CBF1-CCAN bound to centromeric C0N3 DNAタンパク質・ペプチド : x 21種DNA : x 2種 詳細 キーワード kinetochore (動原体) / point centromere / CENP-A nucleosome / topological entrapment / centromeric DNA / CELL CYCLE (細胞周期)機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
Cbf1-Met4-Met28 complex / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / 2-micrometer circle DNA / 2-micrometer plasmid partitioning / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / RAVE complex / Iron uptake and transport ... Cbf1-Met4-Met28 complex / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / 2-micrometer circle DNA / 2-micrometer plasmid partitioning / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / RAVE complex / Iron uptake and transport / CBF3 complex / regulation of transcription by galactose / regulation of sulfur amino acid metabolic process / cellular response to methylmercury / meiotic sister chromatid segregation / Mis6-Sim4 complex / vacuolar proton-transporting V-type ATPase complex assembly / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / centromere complex assembly / establishment of meiotic sister chromatid cohesion / septin ring assembly / HDMs demethylate histones / ascospore formation / HATs acetylate histones / Condensation of Prophase Chromosomes / RNA polymerase I upstream activating factor complex / attachment of spindle microtubules to kinetochore / centromeric DNA binding / CENP-A containing chromatin assembly / regulation of exit from mitosis / Antigen processing: Ubiquitination & Proteasome degradation / outer kinetochore / protein localization to chromosome, centromeric region / SUMOylation of chromatin organization proteins / establishment of mitotic sister chromatid cohesion / vacuolar acidification / kinetochore assembly / condensed chromosome, centromeric region / regulation of metabolic process / exit from mitosis / replication fork protection complex / spindle pole body / protein localization to kinetochore / RMTs methylate histone arginines / positive regulation of glucose transmembrane transport / protein neddylation / postreplication repair / mitotic intra-S DNA damage checkpoint signaling / silent mating-type cassette heterochromatin formation / mitochondrial fusion / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / DNA binding, bending / SCF複合体 / mitotic spindle assembly checkpoint signaling / positive regulation of transcription by RNA polymerase I / DNA replication origin binding / nucleolar large rRNA transcription by RNA polymerase I / mitotic sister chromatid segregation / cullin family protein binding / rRNA transcription / subtelomeric heterochromatin formation / chromosome, centromeric region / DNA replication initiation / 細胞内膜系 / regulation of protein-containing complex assembly / protein localization to CENP-A containing chromatin / negative regulation of cytoplasmic translation / CENP-A containing nucleosome / regulation of mitotic cell cycle / meiotic cell cycle / chromosome segregation / G1/S transition of mitotic cell cycle / 動原体 / DNA-binding transcription repressor activity, RNA polymerase II-specific / nucleosome assembly / structural constituent of chromatin / G2/M transition of mitotic cell cycle / ヌクレオソーム / mitotic cell cycle / chromatin organization / ubiquitin-dependent protein catabolic process / DNA-binding transcription activator activity, RNA polymerase II-specific / protein-containing complex assembly / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / chromosome, telomeric region / protein ubiquitination / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / クロマチンリモデリング / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein heterodimerization activity / 細胞分裂 / DNA修復 / protein-containing complex binding / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / structural molecule activity / negative regulation of transcription by RNA polymerase II 類似検索 - 分子機能 : / Kinetochore subunit Nkp2/Cnl2 / Cnl2/NKP2 family protein / : / : / CENPH protein / CENPU protein / Centromere DNA-binding protein complex CBF3 subunit B, C-terminal / Centromere DNA-binding protein complex CBF3 subunit B / Centromere protein O ... : / Kinetochore subunit Nkp2/Cnl2 / Cnl2/NKP2 family protein / : / : / CENPH protein / CENPU protein / Centromere DNA-binding protein complex CBF3 subunit B, C-terminal / Centromere DNA-binding protein complex CBF3 subunit B / Centromere protein O / Cenp-O kinetochore centromere component / Centromere protein Chl4/mis15/CENP-N / Kinetochore protein CHL4 like / Zn(2)-C6 fungal-type DNA-binding domain signature. / Fungal Zn(2)-Cys(6) binuclear cluster domain / Zn(2)-C6 fungal-type DNA-binding domain superfamily / Zn(2)-C6 fungal-type DNA-binding domain profile. / GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain / Zn(2)-C6 fungal-type DNA-binding domain / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / Helix-loop-helix DNA-binding domain / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / Helix-loop-helix DNA-binding domain superfamily / helix loop helix domain / Myc-type, basic helix-loop-helix (bHLH) domain / Myc-type, basic helix-loop-helix (bHLH) domain profile. / SKP1/BTB/POZ domain superfamily / Histone H2B signature. / ヒストンH2B / ヒストンH2B / Histone H2A conserved site / Histone H2A signature. / Histone H2A, C-terminal domain / C-terminus of histone H2A / ヒストンH2A / Histone 2A / Histone H4, conserved site / Histone H4 signature. / ヒストンH4 / ヒストンH4 / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / Leucine-rich repeat domain superfamily / Histone H3 signature 2. / ヒストンH3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Histone-fold 類似検索 - ドメイン・相同性 Histone H2B.1 / ヒストンH4 / Histone H2A.1 / Centromere-binding protein 1 / Centromere DNA-binding protein complex CBF3 subunit C / Histone H3-like centromeric protein CSE4 / Inner kinetochore subunit IML3 / Inner kinetochore subunit AME1 / Inner kinetochore subunit CHL4 / Centromere DNA-binding protein complex CBF3 subunit B ... Histone H2B.1 / ヒストンH4 / Histone H2A.1 / Centromere-binding protein 1 / Centromere DNA-binding protein complex CBF3 subunit C / Histone H3-like centromeric protein CSE4 / Inner kinetochore subunit IML3 / Inner kinetochore subunit AME1 / Inner kinetochore subunit CHL4 / Centromere DNA-binding protein complex CBF3 subunit B / Inner kinetochore subunit CNN1 / Inner kinetochore subunit MCM22 / Suppressor of kinetochore protein 1 / Inner kinetochore subunit OKP1 / Inner kinetochore subunit CTF19 / Inner kinetochore subunit NKP2 / Inner kinetochore subunit MCM21 / Inner kinetochore subunit MCM16 / Inner kinetochore subunit NKP1 / Inner kinetochore subunit CTF3 / Inner kinetochore subunit WIP1 類似検索 - 構成要素生物種 Saccharomyces cerevisiae (パン酵母)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.7 Å 詳細 データ登録者Dendooven TD / Zhang Z / Yang J / McLaughlin S / Schwabb J / Scheres S / Yatskevich S / Barford D 資金援助 英国, ドイツ, 4件 詳細 詳細を隠すOrganization Grant number 国 UK Research and Innovation (UKRI) MC_UP_1201/6 英国 Cancer Research UK C576/A14109 英国 UK Research and Innovation (UKRI) MC_UP_ A025_1013 英国 Boehringer Ingelheim Fonds (BIF) ドイツ
引用ジャーナル : Sci Adv / 年 : 2023タイトル : Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere.著者 : Tom Dendooven / Ziguo Zhang / Jing Yang / Stephen H McLaughlin / Johannes Schwab / Sjors H W Scheres / Stanislau Yatskevich / David Barford / 要旨 : The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) ... The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A). This revealed a central CENP-A with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (CDEI) motif by CBF1. The two CCAN protomers are linked through CBF3 forming an arch-like configuration. With a structural mechanism for how CENP-A can also be linked to KMN involving only CENP-QU, we present a model for inner kinetochore assembly onto a point centromere and how it organizes the outer kinetochore for chromosome attachment to the mitotic spindle. 履歴 登録 2023年4月26日 - ヘッダ(付随情報) 公開 2023年8月9日 - マップ公開 2023年8月9日 - 更新 2023年8月9日 - 現状 2023年8月9日 処理サイト : PDBe / 状態 : 公開
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