+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-1261 | |||||||||
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タイトル | Following the signal sequence from ribosomal tunnel exit to signal recognition particle. | |||||||||
マップデータ | E. coli signal recognition particle bound to ribosome nascent chain complex | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 absorption of visible light / G protein-coupled photoreceptor activity / シグナル認識粒子 / photoreceptor inner segment membrane / rhodopsin mediated signaling pathway / 11-cis retinal binding / signal-recognition-particle GTPase / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / protein targeting to membrane ...absorption of visible light / G protein-coupled photoreceptor activity / シグナル認識粒子 / photoreceptor inner segment membrane / rhodopsin mediated signaling pathway / 11-cis retinal binding / signal-recognition-particle GTPase / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / protein targeting to membrane / negative regulation of cytoplasmic translational initiation / photoreceptor outer segment membrane / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / translational termination / DnaA-L2 complex / translation repressor activity / translational initiation / negative regulation of DNA-templated DNA replication initiation / ribosome assembly / 視覚 / mRNA regulatory element binding translation repressor activity / response to reactive oxygen species / assembly of large subunit precursor of preribosome / cytosolic ribosome assembly / regulation of cell growth / DNA-templated transcription termination / response to radiation / mRNA 5'-UTR binding / ribosomal large subunit assembly / photoreceptor disc membrane / large ribosomal subunit rRNA binding / ribosome binding / large ribosomal subunit / cytoplasmic translation / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / リボソーム / structural constituent of ribosome / ribonucleoprotein complex / 翻訳 (生物学) / response to antibiotic / mRNA binding / GTPase activity / negative regulation of DNA-templated transcription / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / zinc ion binding / 生体膜 / metal ion binding / 細胞膜 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | |||||||||
生物種 | Escherichia coli (大腸菌) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 9.5 Å | |||||||||
データ登録者 | Halic M / Blau M / Becker T / Mielke T / Pool MR / Wild K / Sinning I / Beckmann R | |||||||||
引用 | ジャーナル: Nature / 年: 2006 タイトル: Following the signal sequence from ribosomal tunnel exit to signal recognition particle. 著者: Mario Halic / Michael Blau / Thomas Becker / Thorsten Mielke / Martin R Pool / Klemens Wild / Irmgard Sinning / Roland Beckmann / 要旨: Membrane and secretory proteins can be co-translationally inserted into or translocated across the membrane. This process is dependent on signal sequence recognition on the ribosome by the signal ...Membrane and secretory proteins can be co-translationally inserted into or translocated across the membrane. This process is dependent on signal sequence recognition on the ribosome by the signal recognition particle (SRP), which results in targeting of the ribosome-nascent-chain complex to the protein-conducting channel at the membrane. Here we present an ensemble of structures at subnanometre resolution, revealing the signal sequence both at the ribosomal tunnel exit and in the bacterial and eukaryotic ribosome-SRP complexes. Molecular details of signal sequence interaction in both prokaryotic and eukaryotic complexes were obtained by fitting high-resolution molecular models. The signal sequence is presented at the ribosomal tunnel exit in an exposed position ready for accommodation in the hydrophobic groove of the rearranged SRP54 M domain. Upon ribosome binding, the SRP54 NG domain also undergoes a conformational rearrangement, priming it for the subsequent docking reaction with the NG domain of the SRP receptor. These findings provide the structural basis for improving our understanding of the early steps of co-translational protein sorting. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_1261.map.gz | 14.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-1261-v30.xml emd-1261.xml | 7 KB 7 KB | 表示 表示 | EMDBヘッダ |
画像 | 1261.gif | 36.6 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-1261 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1261 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_1261.map.gz / 形式: CCP4 / 大きさ: 94.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | E. coli signal recognition particle bound to ribosome nascent chain complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : E. coli signal recognition particle bound to ribosome nascent cha...
全体 | 名称: E. coli signal recognition particle bound to ribosome nascent chain complex |
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要素 |
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-超分子 #1000: E. coli signal recognition particle bound to ribosome nascent cha...
超分子 | 名称: E. coli signal recognition particle bound to ribosome nascent chain complex タイプ: sample / ID: 1000 / Number unique components: 2 |
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-超分子 #1: ribosome
超分子 | 名称: ribosome / タイプ: complex / ID: 1 / 組換発現: No / Ribosome-details: ribosome-prokaryote: ALL |
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由来(天然) | 生物種: Escherichia coli (大腸菌) |
-分子 #1: signal recognition particle
分子 | 名称: signal recognition particle / タイプ: protein_or_peptide / ID: 1 / Name.synonym: srp / 組換発現: No |
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由来(天然) | 生物種: Escherichia coli (大腸菌) |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
凍結 | 凍結剤: ETHANE |
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-電子顕微鏡法
顕微鏡 | FEI TECNAI F30 |
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電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELDBright-field microscopy |
試料ステージ | 試料ホルダー: f / 試料ホルダーモデル: OTHER |
実験機器 | モデル: Tecnai F30 / 画像提供: FEI Company |
-画像解析
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 9.5 Å / 解像度の算出法: FSC 0.5 CUT-OFF |
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