+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDF94 |
---|---|
Sample | Insulin glulisine (Apidra), oligomeric composition
|
Citation | Date: 2019 Jul Title: The quaternary structure of insulin glargine and glulisine under formulation conditions Authors: Nagel N / Graewert M / Gao M / Heyse W / Jeffries C / Svergun D |
Contact author |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Models
Model #2919 | Type: atomic / Comment: monomeric unit / Chi-square value: 1.03 / P-value: 0.574548 Search similar-shape structures of this assembly by Omokage search (details) |
---|---|
Model #2920 | Type: atomic / Comment: dimeric unit / Chi-square value: 1.03 / P-value: 0.574548 Search similar-shape structures of this assembly by Omokage search (details) |
Model #2921 | Type: atomic / Comment: hexameric unit / Chi-square value: 1.03 / P-value: 0.574548 Search similar-shape structures of this assembly by Omokage search (details) |
Model #2922 | Type: atomic / Chi-square value: 1.03 / P-value: 0.574548 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Insulin glulisine (Apidra), oligomeric composition / Specimen concentration: 3.49 mg/ml |
---|---|
Buffer | Name: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20) pH: 7.3 |
Entity #1567 | Type: protein / Description: Insulin glulisine / Formula weight: 5.811 / Num. of mol.: 6 Sequence: GIVEQCCTSI CSLYQLENYC NFVKQHLCGS HLVEALYLVC GERGFFYTPE T |
-Experimental information
Beam | Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1241 Å / Dist. spec. to detc.: 3.1 mm | |||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||
Scan | Title: Insulin glulisine (Apidra), oligomeric composition / Measurement date: Apr 20, 2017 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/A /
| |||||||||||||||||||||||||||||
Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 1225 /
| |||||||||||||||||||||||||||||
Result | Type of curve: single_conc Comments: Here, Apidra measured at 3.49 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions in placebo have been deposited in addition. Mixture analysis of the scattering ...Comments: Here, Apidra measured at 3.49 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions in placebo have been deposited in addition. Mixture analysis of the scattering profiles was performed with the program Oligomer. The SAXS data show that Apidra® primarily consists of hexamers, which dissociate into monomers upon dilution. The presence of dimers was not required to fit the experimental data, and their volume fraction was always essentially zero. A small volume fraction is, however, also made up by larger dodecameric species. The distribution between monomer, dimer, hexamer and dodecamer is documented in the logfile. Calculation of the form factors is based on an internal model (Apidra.pdb) for hexamers and 3W80.pdb for dodecamers.
|