SASDFG4: Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)

Basic information

• Entry: Database: SASBDB / ID: SASDFG4

Sample

Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)

• Condensin complex subunit 3-like protein (protein), Ycg1, Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

• Function / homology: Nuclear condensin complex subunit 3, C-terminal domain / Condensin complex subunit 3 / Nuclear condensing complex subunits, C-term domain / condensin complex / mitotic chromosome condensation / Armadillo-like helical / Armadillo-type fold / cell division / Condensin complex subunit 3-like protein
• Biological species: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus)

Contact author

• Karen Manalastas-Cantos (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #2909: ; Type: atomic / Symmetry: P2 / Chi-square value: 1.57 / P-value: 0.000001; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer); Specimen concentration: 1.94 mg/ml
• Buffer: Name: 25 mM Tris 300 mM NaCl 1mM DTT / pH: 7.5

Entity #1566

Name: Ycg1 / Type: protein / Description: Condensin complex subunit 3-like protein / Formula weight: 108.3 / Num. of mol.: 2
Source: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
References: UniProt: G0SHK2
Sequence:

PDNALRTQVT TVFREAQRTT ASHRKLVVTL RKIQEACCYE PATGQKQSGA AAALSEFDED DFNTEFVRCA LRILPVKKSE GVGEKTVRFV SLFLRHAIEK DNELLGDTQM DEDDADNSTM PETPSTRLTS HLMETILPML MAKDKFVRYR AAQLLAHIVN SLDAIDDDLY QKLRAGLLRR IHDKEPMVRA QAVLGLGRLA GNALDEEPGA GDSDLDGDDD GPGGASGVSL MDKLMDVMKN DPSADVRRTL LVNLPITPKT LPSLLERARD QDPATRRAVY SRLLPALGDF RHLSLSMREK LLRWGLRDRD ENVRKAAARL FRERWIEDCA GTPRPEPGQP IELSPPNMDA LLELLERIDV VNSGGENGIA LEAMRGFWEG RPDYRDAMVF DDHFFETLSA ESVFALRTFN DFCRNEAGGK YEALIEEKLP EVTKLAFYLE RYIKVLIDAI KRAEEQGEEE EEDTAEQEFI VEQLLHIALT LDYADEVGRR KMFALLRQTL SIPELPEECT KLTVMVLRDL CAPDAAGEKE FTSVVLEAVA DVHDTIVDED DDDANVPAED DESFVSARSE VSRESTPAPG GGEGGGSNSR KRGRSPTPKM TEEEAAAKAI REIMINMKCL HIVQCMLQNV QGQLQSNDNL ISMLNNLVVP AVRSHEAPVR ERGLVCLGLC ALLDRSLAEE NLTLFIHFFA KGHTALQITA LHILTDILVV HGAQLLSANP TLLKVYVKAL RSGAKHPELQ AVAVVAASKL LLGRVVSDRD VAAELLKTLV VAYFEPASAG NQSVRQALNY FLPVFCYSRA ENQDLMRRVA LDALHTLYNV REGFDDDDAD IDDEMVSLAT IGACLVDWTD PRKVYTPDAG GLQAEKHVNA DVHLDFAKAI LERLEGNVPR EEKKVLASLL SKLYISPASS PDKTRETYRV VSAAVDTNLL PDTTSRNALY KIHVSLGKIV NALDGAVPSV ETASVAGSTG TGIGTTTTAA TGR

Experimental information

• Beam: Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 4 mm
• Detector: Name: Pilatus 2M

Scan

Title: Condensin complex subunit 3-like protein, Ycg1, at 2mg/ml (dimer)
Measurement date: Oct 20, 2016 / Storage temperature: 13.2 °C / Cell temperature: 10.1 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0492	2.5149

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 664 /

	Min	Max
Q	0.107504	1.48703
P(R) point	1	664
R	0	19.02

Result

Type of curve: single_conc

	Experimental	Standard	Porod
MW	243 kDa	204 kDa	290 kDa
Volume	-	-	463 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	15900	15896.5	41
Radius of gyration, Rg	5.47 nm	5.38 nm	0.22

	Min	Max
D	-	19.02
Guinier point	29	93