SASDF24: Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP)

Basic information

• Entry: Database: SASBDB / ID: SASDF24

Sample

Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP)

• E3 ubiquitin-protein ligase XIAP (protein), Homo sapiens

Function / homology

Function:

endopeptidase regulator activity / regulation of apoptosis involved in tissue homeostasis / inhibition of cysteine-type endopeptidase activity / positive regulation of protein linear polyubiquitination / regulation of BMP signaling pathway / copper ion homeostasis / nucleotide-binding oligomerization domain containing 1 signaling pathway / regulation of nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway / nucleotide-binding oligomerization domain containing 2 signaling pathway / SMAC, XIAP-regulated apoptotic response / Regulation of the apoptosome activity / Activation of caspases through apoptosome-mediated cleavage / SMAC (DIABLO) binds to IAPs / SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes / TNFR1-induced proapoptotic signaling / RIPK1-mediated regulated necrosis / cysteine-type endopeptidase inhibitor activity / protein serine/threonine kinase binding / regulation of innate immune response / cysteine-type endopeptidase inhibitor activity involved in apoptotic process / protein K63-linked ubiquitination / negative regulation of tumor necrosis factor-mediated signaling pathway / positive regulation of type I interferon production / Regulation of PTEN localization / TNFR1-induced NF-kappa-B signaling pathway / positive regulation of protein ubiquitination / Deactivation of the beta-catenin transactivating complex / Regulation of TNFR1 signaling / positive regulation of JNK cascade / RING-type E3 ubiquitin transferase / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / Regulation of necroptotic cell death / Wnt signaling pathway / Regulation of PTEN stability and activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / positive regulation of canonical Wnt signaling pathway / regulation of cell population proliferation / regulation of inflammatory response / regulation of apoptotic process / neuron apoptotic process / positive regulation of canonical NF-kappaB signal transduction / regulation of cell cycle / defense response to bacterium / DNA damage response / negative regulation of apoptotic process / nucleoplasm / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

XIAP/BIRC8, UBA domain / : / BIRC2/3-like, UBA domain / BIR repeat. / BIR repeat / Inhibitor of Apoptosis domain / BIR repeat profile. / Baculoviral inhibition of apoptosis protein repeat / Zinc finger, C3HC4 type (RING finger) / Death-like domain superfamily / Ring finger / Zinc finger RING-type profile. / Zinc finger, RING-type

Component:

E3 ubiquitin-protein ligase XIAP

• Biological species: Homo sapiens (human)
• Citation: Date: 2019 Sep 1; Title: Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach; Authors: Polykretis P / Luchinat E / Bonucci A / Giachetti A / Graewert M / Svergun D

Contact author

• Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #2865: ; Type: atomic / Chi-square value: 1.528 / P-value: 0.000053; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP); Specimen concentration: 7.5 mg/ml
• Buffer: Name: Xiap buffer / pH: 7.4 / Comment: 20 mM Tris buffer, 0.5 mM TCEP,

Entity #1534

Type: protein / Description: E3 ubiquitin-protein ligase XIAP / Formula weight: 56.684 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: P98170
Sequence:

MTFNSFEGSK TCVPADINKE EEFVEEFNRL KTFANFPSGS PVSASTLARA GFLYTGEGDT VRCFSCHAAV DRWQYGDSAV GRHRKVSPNC RFINGFYLEN SATQSTNSGI QNGQYKVENY LGSRDHFALD RPSETHADYL LRTGQVVDIS DTIYPRNPAM YSEEARLKSF QNWPDYAHLT PRELASAGLY YTGIGDQVQC FCCGGKLKNW EPCDRAWSEH RRHFPNCFFV LGRNLNIRSE SDAVSSDRNF PNSTNLPRNP SMADYEARIF TFGTWIYSVN KEQLARAGFY ALGEGDKVKC FHCGGGLTDW KPSEDPWEQH AKWYPGCKYL LEQKGQEYIN NIHLTHSLEE CLVRTTEKTP SLTRRIDDTI FQNPMVQEAI RMGFSFKDIK KIMEEKIQIS GSNYKSLEVL VADLVNAQKD SMQDESSQTS LQKEISTEEQ LRRLQEEKLC KICMDRNIAI VFVPCGHLVT CKQCAEAVDK CPMCYTVITF KQKIFMS

Experimental information

• Beam: Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
• Detector: Name: Pilatus 2M

Scan

Title: Full-length X-chromosome linked inhibitor of apoptosis protein (XIAP)
Measurement date: Sep 15, 2017 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 15 / Unit: 1/nm /

	Min	Max
Q	0.0912	5.064

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 686 /

	Min	Max
Q	0.182228	2.07258
P(R) point	1	686
R	0	12.82

Result

Type of curve: sec /

	Experimental	Porod
MW	115 kDa	130 kDa
Volume	-	207 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	16800	16438	32
Radius of gyration, Rg	3.99 nm	3.86 nm	0.06

	Min	Max
D	-	12.82
Guinier point	34	89