+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDDH3 |
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Sample | Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2)
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Function / homology | Transcription regulator TrmB, N-terminal / Sugar-specific transcriptional regulator TrmB / helix_turn_helix, Arsenical Resistance Operon Repressor / HTH ArsR-type DNA-binding domain / ArsR-like helix-turn-helix domain / Winged helix DNA-binding domain superfamily / DNA-binding transcription factor activity / Winged helix-like DNA-binding domain superfamily / Transcriptional regulator Lrs14-like protein Function and homology information |
Biological species | Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (acidophilic) |
Citation | Journal: Acta Crystallogr D Struct Biol / Year: 2018 Title: Crystal structure of an Lrs14-like archaeal biofilm regulator from Sulfolobus acidocaldarius. Authors: Marian S Vogt / Simon L Völpel / Sonja Verena Albers / Lars Oliver Essen / Ankan Banerjee / Abstract: The small winged helix-turn-helix (wHTH) proteins of the Lrs14 family are major transcriptional regulators and act as archaeal biofilm regulators (AbfRs) in the crenarchaeote Sulfolobus ...The small winged helix-turn-helix (wHTH) proteins of the Lrs14 family are major transcriptional regulators and act as archaeal biofilm regulators (AbfRs) in the crenarchaeote Sulfolobus acidocaldarius. Here, the first crystal structure of an AbfR ortholog, AbfR2, the deletion of which is known to impair biofilm formation, is presented. Like most other wHTH orthologs, AbfR2 is dimeric in solution as well as in its 2.45 Å resolution crystal structure. Given the presence of three independent AbfR2 dimers in the asymmetric unit, the crystal structure shows a considerable degree of conformational variation within the dimer, the antiparallel orientations of which are stabilized by coiled-coil interaction between H4 helices. Conserved anchor interactions between helices H0 and H4 of AbfR2 further contribute to dimer stabilization. The combined structural and bioinformatic analysis reveals cluster-specific structural differences between different members of the Lrs14 protein family. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1783 | Type: dummy / Radius of dummy atoms: 2.10 A / Chi-square value: 0.812 / P-value: 0.101902 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #1786 | Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 1.052 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) Specimen concentration: 0.83 mg/ml |
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Buffer | Name: 300 mM NaCl, 20 mM HEPES, pH 7.5 / pH: 7.5 |
Entity #919 | Name: AbfR2 / Type: protein / Description: Transcriptional regulator Lrs14-like protein / Formula weight: 16.368 / Num. of mol.: 2 Source: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) References: UniProt: Q4J9G1 Sequence: MGSSHHHHHH SQDPNSMETV LQIPYQKKTQ IEKLLEFMYG LNEKEVQLIF RLLYSDTKLN IEELAEEFKV SKALISKSLS ELANKGLIER EKVSNEGRKG RPIYVYYVDR EQLFKRISRD LEELVQASIA KLKEYIFKS |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | |||||||||||||||||||||||||||||||||
Scan | Title: Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) Measurement date: May 5, 2016 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 449 /
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Result | Type of curve: single_conc Comments: The associated atomic model structure is included for comparative purposes only, i.e., to compare the overall topology of the atomic model with the solution scattering and the DAMMIF bead ...Comments: The associated atomic model structure is included for comparative purposes only, i.e., to compare the overall topology of the atomic model with the solution scattering and the DAMMIF bead model. Two fits displayed for the atomic model were calculated using CRYSOL (top) and SASREF (bottom).
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