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Yorodumi- SASDC25: RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDC25 |
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Sample | RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex
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Function / homology | Function and homology information : / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / RNA catabolic process / glycolytic process / RNA helicase activity / RNA helicase / magnesium ion binding / cell surface ...: / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / RNA catabolic process / glycolytic process / RNA helicase activity / RNA helicase / magnesium ion binding / cell surface / RNA binding / extracellular region / ATP binding / cytoplasm Similarity search - Function |
Biological species | Escherichia coli (E. coli) |
Citation | Date: 2017 Nov 9 Title: Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes Authors: Bruce H / Du D / Matak-Vinkovic D / Bandyra K / Broadhurst R / Martin E / Sobott F / Shkumatov A |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDC25 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-External links
Related items in Molecule of the Month |
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-Models
Model #1314 | Type: dummy / Software: (2.3) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Best fitting model out of 10 independent reconstructions Chi-square value: 4.97 / P-value: 0.000001 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex Specimen concentration: 9.1 mg/ml / Entity id: 697 / 698 / 699 |
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Buffer | Name: 50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v) pH: 7.5 |
Entity #697 | Name: RNase E 603-850 / Type: protein / Description: RNase E 603-850 / Formula weight: 30.127 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: Q46977 Sequence: ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV ...Sequence: ERQQDRRKPR QNNRRDRNER RDTRSERTEG SDNREENRRN RRQAQQQTAE TRESRQQAEV TEKARTADEQ QAPRRERSRR RNDDKRQAQQ EAKALNVEEQ SVQETEQEER VRPVQPRRKQ RQLNQKVRYE QSVAEEAVVA PVVEETVAAE PIVQEAPAPR TELVKVPLPV VAQTAPEQQE ENNADNRDNG GMPRRSRRSP RHLRVSGQRR RRYRDERYPT QSPMPLTVAC ASPELASGKV WIRYPIVRHH HHHH |
Entity #698 | Name: RhlB / Type: protein / Description: ATP-dependent RNA helicase RhlB / Formula weight: 47.125 / Num. of mol.: 1 / Source: Escherichia coli / References: UniProt: W8ZZD4 Sequence: MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM ...Sequence: MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT RPRTGNGPRR TGAPRNRRRS G |
Entity #699 | Name: Enolase / Type: protein / Description: Enolase / Formula weight: 45.654 / Num. of mol.: 2 / Source: Escherichia coli / References: UniProt: A1AEW7 Sequence: MSKIVKIIGR EIIDSRGNPT VEAEVHLEGG FVGMAAAPSG ASTGSREALE LRDGDKSRFL GKGVTKAVAA VNGPIAQALI GKDAKDQAGI DKIMIDLDGT ENKSKFGANA ILAVSLANAK AAAAAKGMPL YEHIAELNGT PGKYSMPVPM MNIINGGEHA DNNVDIQEFM ...Sequence: MSKIVKIIGR EIIDSRGNPT VEAEVHLEGG FVGMAAAPSG ASTGSREALE LRDGDKSRFL GKGVTKAVAA VNGPIAQALI GKDAKDQAGI DKIMIDLDGT ENKSKFGANA ILAVSLANAK AAAAAKGMPL YEHIAELNGT PGKYSMPVPM MNIINGGEHA DNNVDIQEFM IQPVGAKTVK EAIRMGSEVF HHLAKVLKAK GMNTAVGDEG GYAPNLGSNA EALAVIAEAV KAAGYELGKD ITLAMDCAAS EFYKDGKYVL AGEGNKAFTS EEFTHFLEEL TKQYPIVSIE DGLDESDWDG FAYQTKVLGD KIQLVGDDLF VTNTKILKEG IEKGIANSIL IKFNQIGSLT ETLAAIKMAK DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA PYNGRKEIKG QA |
-Experimental information
Beam | Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1022 Å / Dist. spec. to detc.: 1.79 mm | ||||||||||||||||||||||||||||||||||||
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Detector | Name: AVIEX / Type: CCD | ||||||||||||||||||||||||||||||||||||
Scan | Title: RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex Measurement date: Jul 16, 2014 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1.5 sec. / Number of frames: 250 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 873 /
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Result | Type of curve: other /
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