+Open data
-Basic information
Entry | Database: PDB / ID: 9eqh | ||||||
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Title | WWP2 WW2-2,3-linker-HECT (WWP2-LH) | ||||||
Components | Isoform 2 of NEDD4-like E3 ubiquitin-protein ligase WWP2 | ||||||
Keywords | LIGASE / Ubiquitin | ||||||
Function / homology | Function and homology information negative regulation of protein transport / extracellular transport / regulation of potassium ion transmembrane transporter activity / HECT-type E3 ubiquitin transferase / negative regulation of transporter activity / regulation of monoatomic ion transmembrane transport / negative regulation of Notch signaling pathway / RHOJ GTPase cycle / RHOQ GTPase cycle / protein K63-linked ubiquitination ...negative regulation of protein transport / extracellular transport / regulation of potassium ion transmembrane transporter activity / HECT-type E3 ubiquitin transferase / negative regulation of transporter activity / regulation of monoatomic ion transmembrane transport / negative regulation of Notch signaling pathway / RHOJ GTPase cycle / RHOQ GTPase cycle / protein K63-linked ubiquitination / RHOU GTPase cycle / transcription factor binding / protein autoubiquitination / ubiquitin ligase complex / regulation of membrane potential / NOTCH3 Activation and Transmission of Signal to the Nucleus / protein modification process / negative regulation of DNA-binding transcription factor activity / Regulation of PTEN stability and activity / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / proteasome-mediated ubiquitin-dependent protein catabolic process / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / protein ubiquitination / symbiont entry into host cell / negative regulation of gene expression / negative regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / extracellular exosome / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.05 Å | ||||||
Authors | Dudey, A.P. / Hemmings, A.M. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: To Be Published Title: Expanding the Inhibitor Space of the WWP1 and WWP2 HECT E3 Ligases Authors: Dudey, A.P. / Rigby, J.M. / Hughes, G.R. / Stephenson, G.R. / Storr, T.E. / Chantry, A. / Hemmings, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 9eqh.cif.gz | 188.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb9eqh.ent.gz | 144.9 KB | Display | PDB format |
PDBx/mmJSON format | 9eqh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/eq/9eqh ftp://data.pdbj.org/pub/pdb/validation_reports/eq/9eqh | HTTPS FTP |
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-Related structure data
Related structure data | 9eqkC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 53441.539 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: WWP2 / Plasmid: pGEX6p-2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 Codon Plus RP References: UniProt: O00308, HECT-type E3 ubiquitin transferase | ||||||
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#2: Chemical | #3: Chemical | ChemComp-NA / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.6 % / Description: Square Plates |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: 100 mM MMT pH 6.0, 25 % PEG1500 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.9795 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 24, 2023 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.046→70.079 Å / Num. obs: 28618 / % possible obs: 99.5 % / Redundancy: 6.7 % / Biso Wilson estimate: 33.2 Å2 / CC1/2: 0.994 / Rmerge(I) obs: 0.19 / Rpim(I) all: 0.075 / Rrim(I) all: 0.205 / Net I/σ(I): 6.9 |
Reflection shell | Resolution: 2.046→5.552 Å / Num. unique obs: 1419 / CC1/2: 0.486 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.05→70.079 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.916 / SU B: 8.866 / SU ML: 0.223 / Cross valid method: THROUGHOUT / ESU R: 0.241 / ESU R Free: 0.211 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.688 Å2
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Refinement step | Cycle: LAST / Resolution: 2.05→70.079 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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