+Open data
-Basic information
Entry | Database: PDB / ID: 8x7t | |||||||||
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Title | MCM in the Apo state. | |||||||||
Components | mini-chromosome maintenance complex 3 | |||||||||
Keywords | HYDROLASE / Helicase / Replication | |||||||||
Biological species | Thermococcus kodakarensis (archaea) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.26 Å | |||||||||
Authors | Ma, J. / Yi, G. / Ye, M. / MacGregor-Chatwin, C. / Sheng, Y. / Lu, Y. / Li, M. / Gilbert, R.J.C. / Zhang, P. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: To Be Published Title: MCM in the Apo state Authors: Ma, J. / Yi, G. / Ye, M. / MacGregor-Chatwin, C. / Sheng, Y. / Lu, Y. / Li, M. / Gilbert, R.J.C. / Zhang, P. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8x7t.cif.gz | 684.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8x7t.ent.gz | 566.2 KB | Display | PDB format |
PDBx/mmJSON format | 8x7t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x7/8x7t ftp://data.pdbj.org/pub/pdb/validation_reports/x7/8x7t | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 76631.242 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermococcus kodakarensis (archaea) / Production host: Escherichia coli (E. coli) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: MCM homohexamer / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Thermococcus kodakarensis (archaea) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 7.8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 3000 nm / Nominal defocus min: 500 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
EM software | Name: PHENIX / Version: 1.14_3260: / Category: model refinement |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 3.26 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 445096 / Symmetry type: POINT |