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- PDB-8wbu: The crystal structure of circular mannose with mutant H247F of th... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8wbu | ||||||
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Title | The crystal structure of circular mannose with mutant H247F of the cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus | ||||||
![]() | Cellobiose 2-epimerase![]() | ||||||
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Function / homology | ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yang, R.J. / Feng, Y.H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The crystal structure of circular mannose with mutant H247F of the cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus Authors: Yang, R.J. / Feng, Y.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 186 KB | Display | ![]() |
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PDB format | ![]() | 145.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | ![]() Mass: 46623.121 Da / Num. of mol.: 1 / Mutation: H247F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 43494 / DSM 8903 / Tp8T 6331 / Gene: Csac_0294 / Production host: ![]() ![]() ![]() | ||||
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#2: Sugar | ![]() #3: Water | ChemComp-HOH / | ![]() Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.25 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.8 M NaCl, 0.1 M Tris-HCl pH 8.5, 32% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 193 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.7→50 Å / Num. obs: 42563 / % possible obs: 98.6 % / Observed criterion σ(I): -3 / Redundancy: 4.6 % / CC1/2: 0.999 / Rmerge(I) obs: 0.068 / Net I/σ(I): 13.64 |
Reflection shell | Resolution: 1.7→1.74 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.767 / Mean I/σ(I) obs: 2.15 / Num. unique obs: 3127 / CC1/2: 0.678 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→34.851 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 1.2909 Å / Origin y: -0.3951 Å / Origin z: -8.164 Å
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Refinement TLS group | Selection details: all |