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Yorodumi- PDB-8uh1: Crystal structure of T. brucei EIF4E6 in complex with EIF4G5 peptide -
+Open data
-Basic information
Entry | Database: PDB / ID: 8uh1 | ||||||||||||
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Title | Crystal structure of T. brucei EIF4E6 in complex with EIF4G5 peptide | ||||||||||||
Components |
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Keywords | TRANSLATION / Translation initiation factor / eIF4E family / EIF4E6 / EIF4G5 | ||||||||||||
Function / homology | Function and homology information RNA cap 4 binding / motile cilium assembly / eukaryotic translation initiation factor 4F complex / RNA 7-methylguanosine cap binding / post-transcriptional regulation of gene expression / translation initiation factor activity / cell motility / mRNA binding / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Trypanosoma brucei (eukaryote) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.898 Å | ||||||||||||
Authors | Guimaraes, B.G. / Penteado, R.F. | ||||||||||||
Funding support | France, Brazil, 3items
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Citation | Journal: Sci Rep / Year: 2024 Title: Structural analysis of the Trypanosoma brucei EIF4E6/EIF4G5 complex reveals details of the interaction between unusual eIF4F subunits. Authors: Ferras Penteado, R. / Santana da Silva, R. / Nascimento Moura, D.M. / Barbosa de Lima, G. / Muniz Malvezzi, A. / Tamara da Silva Monteiro, T. / Cavalcanti Xavier, C. / Vichier-Guerre, S. / ...Authors: Ferras Penteado, R. / Santana da Silva, R. / Nascimento Moura, D.M. / Barbosa de Lima, G. / Muniz Malvezzi, A. / Tamara da Silva Monteiro, T. / Cavalcanti Xavier, C. / Vichier-Guerre, S. / Dugue, L. / Pochet, S. / Tonin Zanchin, N.I. / de Souza Reis, C.R. / Pompilio de Melo Neto, O. / Gomes Guimaraes, B. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8uh1.cif.gz | 96.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8uh1.ent.gz | 72.4 KB | Display | PDB format |
PDBx/mmJSON format | 8uh1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uh/8uh1 ftp://data.pdbj.org/pub/pdb/validation_reports/uh/8uh1 | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 21800.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei (eukaryote) / Production host: Escherichia coli (E. coli) / References: UniProt: Q57V43 |
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#2: Protein/peptide | Mass: 4769.366 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma brucei (eukaryote) / Production host: Escherichia coli (E. coli) / References: UniProt: Q57VY5 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion Details: Complex at 12 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 33% Precipitant Mix 4, 0.09 M NPS, 0.12 M Ethylene ...Details: Complex at 12 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 33% Precipitant Mix 4, 0.09 M NPS, 0.12 M Ethylene glycols, 0.1 M Buffer System 3 (pH 8.5), from Morpheus Screen mixes |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.978565 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 11, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978565 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→44 Å / Num. obs: 16430 / % possible obs: 95.1 % / Redundancy: 13.3 % / CC1/2: 0.99 / Net I/σ(I): 13.2 |
Reflection shell | Resolution: 1.9→1.97 Å / Num. unique obs: 822 / CC1/2: 0.54 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.898→44 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.942 / SU R Cruickshank DPI: 0.164 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.171 / SU Rfree Blow DPI: 0.148 / SU Rfree Cruickshank DPI: 0.145
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Displacement parameters | Biso mean: 37.26 Å2
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Refine analyze | Luzzati coordinate error obs: 0.24 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.898→44 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.93 Å
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Refinement TLS params. | Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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