+Open data
-Basic information
Entry | Database: PDB / ID: 8u1r | ||||||
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Title | Prefusion-stabilized Langya virus F protein, variant G99C/I109C | ||||||
Components | Fusion glycoprotein F | ||||||
Keywords | VIRUS / Langya / Langya virus / LayV / fusion / F / LayV F / VIRAL PROTEIN / disulfide / prefusion / prefusion-stabilized / vaccine design | ||||||
Biological species | Langya virus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å | ||||||
Authors | Byrne, P.O. / McLellan, J.S. | ||||||
Funding support | United States, 1items
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Citation | Journal: To Be Published Title: Prefusion-stabilized Langya virus F protein, variant G99C/I109C Authors: Byrne, P.O. / Blade, E.G. / McLellan, J.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8u1r.cif.gz | 210.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8u1r.ent.gz | 168.7 KB | Display | PDB format |
PDBx/mmJSON format | 8u1r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u1/8u1r ftp://data.pdbj.org/pub/pdb/validation_reports/u1/8u1r | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 60590.789 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Details: Prefusion-stabilized Langya virus F protein, variant G99C/I109C Source: (gene. exp.) Langya virus / Production host: Homo sapiens (human) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Prefusion-stabilized Langya virus F protein, variant G99C/I109C Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: Langya virus |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Microscopy | Model: TFS GLACIOS |
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Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 44 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) |
-Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||
Particle selection | Num. of particles selected: 321161 | ||||||||||||
Symmetry | Point symmetry: C3 (3 fold cyclic) | ||||||||||||
3D reconstruction | Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 72726 / Symmetry type: POINT |