+Open data
-Basic information
Entry | Database: PDB / ID: 8ozz | |||||||||
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Title | PH domain of AKT-like kinase in Trypanosoma cruzi | |||||||||
Components | PH domain of Akt-like kinase in Trypanosoma cruzi | |||||||||
Keywords | LIPID BINDING PROTEIN / PH domain / AKT-like/RAC/PKB / kinase / Trypanosoma cruzi | |||||||||
Function / homology | Function and homology information phosphorylation / protein serine/threonine kinase activity / ATP binding Similarity search - Function | |||||||||
Biological species | Trypanosoma cruzi (eukaryote) | |||||||||
Method | SOLUTION NMR / na | |||||||||
Authors | Stadler, K.A. / Ortiz-Joya, L.J. / Zangger, K. / Gubensaek, N. | |||||||||
Funding support | Austria, 2items
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Citation | Journal: Sci Rep / Year: 2024 Title: Structural investigation of Trypanosoma cruzi Akt-like kinase as drug target against Chagas disease. Authors: Stadler, K.A. / Ortiz-Joya, L.J. / Singh Sahrawat, A. / Buhlheller, C. / Gruber, K. / Pavkov-Keller, T. / O'Hagan, T.B. / Guarne, A. / Pulido, S. / Marin-Villa, M. / Zangger, K. / Gubensak, N. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ozz.cif.gz | 354.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ozz.ent.gz | 292.9 KB | Display | PDB format |
PDBx/mmJSON format | 8ozz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oz/8ozz ftp://data.pdbj.org/pub/pdb/validation_reports/oz/8ozz | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13494.111 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trypanosoma cruzi (eukaryote) / Gene: Tc00.1047053509047.110 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q4D6D3 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100,08 mM / Label: Conditions / pH: 6.5 / Pressure: 1,01325 bar / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 700 MHz / Details: TCI Cryo Probe 5 mm |
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-Processing
NMR software |
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Refinement | Method: na / Software ordinal: 2 / Details: fragment selection and full-atom relaxation | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 10000 / Conformers submitted total number: 10 |