[English] 日本語
![](img/lk-miru.gif)
- PDB-8kei: Cryo-EM structure of NADPH oxidase 2 in complex with p22phox and EROS -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8kei | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of NADPH oxidase 2 in complex with p22phox and EROS | ||||||
![]() |
| ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() smooth muscle hypertrophy / cellular response to L-glutamine / positive regulation of toll-like receptor 2 signaling pathway / hypoxia-inducible factor-1alpha signaling pathway / superoxide-generating NAD(P)H oxidase activity / respiratory burst after phagocytosis / positive regulation of defense response to bacterium / mucus secretion / Cross-presentation of particulate exogenous antigens (phagosomes) / perinuclear endoplasmic reticulum ...smooth muscle hypertrophy / cellular response to L-glutamine / positive regulation of toll-like receptor 2 signaling pathway / hypoxia-inducible factor-1alpha signaling pathway / superoxide-generating NAD(P)H oxidase activity / respiratory burst after phagocytosis / positive regulation of defense response to bacterium / mucus secretion / Cross-presentation of particulate exogenous antigens (phagosomes) / perinuclear endoplasmic reticulum / cytochrome complex assembly / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liang, S.Y. / Liu, A.J. / Liu, Y.Z. / Ye, R.D. | ||||||
Funding support | 1items
| ||||||
![]() | ![]() Title: Cryo-EM structure of NADPH oxidase 2 in complex with p22phox and EROS Authors: Liang, S.Y. / Liu, A.J. / Liu, Y.Z. / Ye, R.D. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 244.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 190.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 37159MC M: map data used to model this data C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
-Cytochrome b-245 ... , 3 types, 3 molecules ABD
#1: Protein | ![]() Mass: 14663.218 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|---|
#2: Protein | ![]() Mass: 64853.066 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
#4: Protein | Mass: 18491.500 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
-Antibody , 2 types, 2 molecules CE
#3: Antibody | Mass: 23484.305 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
---|---|
#5: Antibody | Mass: 23521.154 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Sugars , 2 types, 3 molecules ![](data/chem/img/NAG.gif)
#6: Polysaccharide | ![]() Source method: isolated from a genetically manipulated source #9: Sugar | ChemComp-NAG / | ![]() |
---|
-Non-polymers , 3 types, 4 molecules ![](data/chem/img/POV.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/LBN.gif)
![](data/chem/img/HEM.gif)
![](data/chem/img/LBN.gif)
#7: Chemical | ChemComp-POV / (![]() | ||
---|---|---|---|
#8: Chemical | ![]() #10: Chemical | ChemComp-LBN / | ![]() |
-Details
Has ligand of interest | Y |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
-
Sample preparation
Component | Name: NADPH oxidase 2 in complex with Cytochrome b-245 light chain, Cytochrome b-245 chaperone 1 and a monoclonal antibody![]() Type: COMPLEX / Entity ID: #1-#5 / Source: RECOMBINANT | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Source (natural) |
| ||||||||||||
Source (recombinant) | Organism: ![]() ![]() | ||||||||||||
Buffer solution | pH: 7.4 | ||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() | ||||||||||||
Specimen support | Grid material: GOLD | ||||||||||||
Vitrification![]() | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 52 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-
Processing
CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3D reconstruction![]() | Resolution: 3.56 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 131926 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|