male germ-line stem cell population maintenance / sperm flagellum assembly / outer dense fiber / axonemal A tubule inner sheath / manchette assembly / outer acrosomal membrane / axonemal B tubule inner sheath / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium ...male germ-line stem cell population maintenance / sperm flagellum assembly / outer dense fiber / axonemal A tubule inner sheath / manchette assembly / outer acrosomal membrane / axonemal B tubule inner sheath / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / protein localization to motile cilium / left/right pattern formation / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / epithelial cilium movement involved in determination of left/right asymmetry / regulation of calcineurin-NFAT signaling cascade / Intraflagellar transport / inner dynein arm assembly / Carboxyterminal post-translational modifications of tubulin / protein polyglutamylation / cilium-dependent cell motility / sperm axoneme assembly / positive regulation of feeding behavior / cerebrospinal fluid circulation / COPI-independent Golgi-to-ER retrograde traffic / regulation of cilium beat frequency involved in ciliary motility / sperm principal piece / MAP kinase tyrosine/serine/threonine phosphatase activity / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / cilium movement involved in cell motility / epithelial cilium movement involved in extracellular fluid movement / intraciliary transport / 9+2 motile cilium / regulation of store-operated calcium entry / PKR-mediated signaling / COPI-mediated anterograde transport / protein localization to organelle / Aggrephagy / 転移酵素; リンを含む基を移すもの / calcium ion sensor activity / ciliary transition zone / acrosomal membrane / Kinesins / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / RHO GTPases activate IQGAPs / cilium movement / sperm DNA condensation / The role of GTSE1 in G2/M progression after G2 checkpoint / axoneme assembly / Recycling pathway of L1 / axonemal microtubule / cilium organization / left/right axis specification / flagellated sperm motility / COPI-dependent Golgi-to-ER retrograde traffic / gamma-tubulin ring complex / RHO GTPases Activate Formins / Separation of Sister Chromatids / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition / manchette / nucleotide-activated protein kinase complex / positive regulation of cilium assembly / MHC class II antigen presentation / protein targeting to mitochondrion / CTP biosynthetic process / UTP biosynthetic process / 繊毛 / positive regulation of cell motility / protein targeting to membrane / negative regulation of TOR signaling / determination of left/right symmetry / GTP biosynthetic process / 微小管形成中心 / nucleoside diphosphate kinase activity / tubulin complex / regulation of neuron projection development / 中間径フィラメント / myosin phosphatase activity / extrinsic component of membrane / 細胞結合 / protein-serine/threonine phosphatase / beta-tubulin binding / AMP binding / regulation of cell division / receptor clustering / phosphatase activity / axoneme / mitotic cytokinesis / centriolar satellite / cilium assembly 類似検索 - 分子機能
Spermatid-specific manchette-related protein 1 / Testis-expressed sequence 37 protein / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / Spermatid-specific manchette-related protein 1 / TSC21 family / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 ...Spermatid-specific manchette-related protein 1 / Testis-expressed sequence 37 protein / Testis-specific serine/threonine-protein kinase 6, catalytic domain / C10orf82-like / Spermatid-specific manchette-related protein 1 / TSC21 family / Protein phosphatase 1 regulatory subunit 32 / Testis, prostate and placenta-expressed protein / Testis-expressed protein 49 / Protein phosphatase 1 regulatory subunit 32 / Protein of unknown function DUF1143 / Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4541 / Protein of unknown function DUF4513 / Cilia- and flagella-associated protein 68 / Sperm-associated microtubule inner protein 10 / Cilia and flagella associated protein 90 / Sperm-tail PG-rich repeat / DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / Sperm-tail PG-rich repeat / EF-hand domain / Tektin bundle interacting protein 1 / FAM183 family / FAM183A and FAM183B related / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 95 / NDPK7, first NDPk domain / Cilia- and flagella-associated protein 107 / : / Protein CFAP95 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / Nucleoside diphosphate kinase (NDPK)-like domain profile. / EFハンド / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / Nucleoside diphosphate kinase / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / EF-hand domain pair / Mir domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / チューブリン / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain 類似検索 - ドメイン・相同性
GUANOSINE-5'-DIPHOSPHATE / グアノシン三リン酸 / Cilia and flagella associated protein 77 / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Family with sequence similarity 183, member B / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Protein phosphatase 1 regulatory subunit 32 ...GUANOSINE-5'-DIPHOSPHATE / グアノシン三リン酸 / Cilia and flagella associated protein 77 / Cilia- and flagella- associated protein 210 / Tektin bundle-interacting protein 1 / Family with sequence similarity 183, member B / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Protein phosphatase 1 regulatory subunit 32 / Tektin-4 / Spermatid-specific manchette-related protein 1 / Sperm-associated antigen 8 / Cilia- and flagella-associated protein 107 / Piercer of microtubule wall 1 protein / Cilia- and flagella-associated protein 52 / Meiosis-specific nuclear structural protein 1 / Testis, prostate and placenta-expressed protein / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Cilia- and flagella-associated protein 161 / Enkurin / Tektin-3 / Cilia- and flagella-associated protein 20 / Testis expressed 49 / EF-hand domain-containing family member B / Outer dense fiber protein 3 / Tektin-2 / Testis-specific serine/threonine-protein kinase 6 / Cilia- and flagella-associated protein 95 / Uncharacterized protein C10orf82 homolog / RIB43A-like with coiled-coils protein 1 / Cilia- and flagella-associated protein 68 / Cilia- and flagella-associated protein 53 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / RIB43A-like with coiled-coils protein 2 / Dual specificity phosphatase 21 / Cilia- and flagella-associated protein 141 / Sperm-associated microtubule inner protein 10 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 45 / Cilia- and flagella-associated protein 276 / Testis-expressed sequence 37 protein / Tektin-1 / Parkin coregulated gene protein homolog / Cilia- and flagella-associated protein 90 / Ciliary microtubule inner protein 2C / Nucleoside diphosphate kinase homolog 7 / Piercer of microtubule wall 2 protein 類似検索 - 構成要素
National Natural Science Foundation of China (NSFC)
中国
引用
ジャーナル: Cell / 年: 2023 タイトル: Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. 著者: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu ...著者: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu / Miao Gui / Jianping Wu / 要旨: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron ...Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility.