+Open data
-Basic information
Entry | Database: PDB / ID: 8inz | ||||||
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Title | Cryo-EM structure of human HCN3 channel in apo state | ||||||
Components | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 | ||||||
Keywords | MEMBRANE PROTEIN / human HCN3 channel | ||||||
Function / homology | Function and homology information cone cell pedicle / HCN channels / HCN channel complex / cellular response to dopamine / voltage-gated sodium channel activity / regulation of membrane depolarization / voltage-gated potassium channel activity / sodium ion transmembrane transport / cAMP binding / potassium ion transmembrane transport ...cone cell pedicle / HCN channels / HCN channel complex / cellular response to dopamine / voltage-gated sodium channel activity / regulation of membrane depolarization / voltage-gated potassium channel activity / sodium ion transmembrane transport / cAMP binding / potassium ion transmembrane transport / regulation of membrane potential / axon / dendrite / neuronal cell body / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.72 Å | ||||||
Authors | Yu, B. / Lu, Q.Y. / Li, J. / Zhang, J. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Cryo-EM structure of human HCN3 channel in apo state Authors: Yu, B. / Lu, Q.Y. / Li, J. / Zhang, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8inz.cif.gz | 343.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8inz.ent.gz | 273.7 KB | Display | PDB format |
PDBx/mmJSON format | 8inz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/in/8inz ftp://data.pdbj.org/pub/pdb/validation_reports/in/8inz | HTTPS FTP |
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-Related structure data
Related structure data | 35602MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 86142.922 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HCN3 Production host: Mammalian expression vector BsrGI-MCS-pcDNA3.1 (others) References: UniProt: Q9P1Z3 #2: Chemical | ChemComp-VX9 / Mass: 492.774 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C31H56O4 / Feature type: SUBJECT OF INVESTIGATION Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Mammalian expression vector BsrGI-MCS-pcDNA3.1 (others) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2200 nm / Nominal defocus min: 1800 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 2.72 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 71465 / Symmetry type: POINT | ||||||||||||||||||||||||
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