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- PDB-8idp: Crystal structure of reducing-end xylose-releasing exoxylanase in... -

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Basic information

Entry
Database: PDB / ID: 8idp
TitleCrystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus
ComponentsReducing-end xylose-releasing exoxylanase Xyn30A
KeywordsHYDROLASE
Function / homology
Function and homology information


glucosylceramidase activity / sphingolipid metabolic process / xylan catabolic process
Similarity search - Function
Glycosyl hydrolase family 30, TIM-barrel domain / Glycosyl hydrolase family 30 TIM-barrel domain / Glycosyl hydrolase family 30, beta sandwich domain / Glycosyl hydrolase family 30 beta sandwich domain / Glycoside hydrolase family 30 / Glycosyl hydrolase, all-beta / Glycoside hydrolase superfamily
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / Reducing-end xylose-releasing exoxylanase Xyn30A
Similarity search - Component
Biological speciesTalaromyces pinophilus (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å
AuthorsNakamichi, Y. / Watanabe, M. / Fujii, T. / Inoue, H. / Morita, T.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: Proteins / Year: 2023
Title: Crystal structure of reducing-end xylose-releasing exoxylanase in subfamily 7 of glycoside hydrolase family 30.
Authors: Nakamichi, Y. / Watanabe, M. / Fujii, T. / Inoue, H. / Morita, T.
History
DepositionFeb 14, 2023Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0May 17, 2023Provider: repository / Type: Initial release
Revision 1.1Aug 23, 2023Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Reducing-end xylose-releasing exoxylanase Xyn30A
C: Reducing-end xylose-releasing exoxylanase Xyn30A
B: Reducing-end xylose-releasing exoxylanase Xyn30A
D: Reducing-end xylose-releasing exoxylanase Xyn30A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)220,47642
Polymers204,3064
Non-polymers16,17038
Water27,4011521
1
A: Reducing-end xylose-releasing exoxylanase Xyn30A
C: Reducing-end xylose-releasing exoxylanase Xyn30A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)110,31022
Polymers102,1532
Non-polymers8,15720
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17600 Å2
ΔGint157 kcal/mol
Surface area30700 Å2
MethodPISA
2
B: Reducing-end xylose-releasing exoxylanase Xyn30A
D: Reducing-end xylose-releasing exoxylanase Xyn30A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)110,16620
Polymers102,1532
Non-polymers8,01318
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area17200 Å2
ΔGint159 kcal/mol
Surface area31100 Å2
MethodPISA
Unit cell
Length a, b, c (Å)138.457, 120.072, 122.834
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11B-2512-

HOH

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Components

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Protein , 1 types, 4 molecules ACBD

#1: Protein
Reducing-end xylose-releasing exoxylanase Xyn30A


Mass: 51076.543 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Talaromyces pinophilus (fungus) / Gene: TCE0_047r17950 / Production host: Talaromyces pinophilus (fungus) / References: UniProt: A0A0B8MZ29

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Sugars , 7 types, 19 molecules

#2: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1883.668 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2DManpa1-3[DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,11,10/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-g1_d2-e1_e2-f1_g3-h1_g6-j1_h2-i1_j2-k1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#3: Polysaccharide alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1072.964 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3DManpa1-6[DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3/a4-b1_b4-c1_c3-d1_c6-e1_e3-f1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#4: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#5: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1559.386 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-6[DManpa1-3]DManpa1-6[DManpa1-2DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,9,8/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-f1_d2-e1_f3-g1_f6-h1_h2-i1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#6: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#7: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1559.386 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6[DManpa1-3]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,9,8/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-e1_e3-f1_e6-h1_f2-g1_h2-i1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#8: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 7
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 1540 molecules

#9: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: C3H8O3
#10: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL / Polyethylene glycol


Mass: 194.226 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#11: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#12: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1521 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.78 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: PEG 6000, 0.1 M Tris-HCl, 0.2 M lithium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 15, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9 Å / Relative weight: 1
ReflectionResolution: 1.8→45.96 Å / Num. obs: 188427 / % possible obs: 97.9 % / Redundancy: 5 % / CC1/2: 0.994 / Rpim(I) all: 0.05 / Net I/σ(I): 9.3
Reflection shellResolution: 1.8→1.86 Å / Mean I/σ(I) obs: 2.7 / Num. unique obs: 97250 / CC1/2: 0.82 / Rpim(I) all: 0.257

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Processing

Software
NameVersionClassification
XDSMar 15, 2019data scaling
XDSMar 15, 2019data reduction
MOLREP11.6phasing
PHENIX1.12refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.8→40.94 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 17.8 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.1948 9420 5 %0
Rwork0.1651 ---
obs0.1666 188371 99.5 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.8→40.94 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13933 0 1076 1521 16530
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.008
X-RAY DIFFRACTIONf_angle_d0.894
X-RAY DIFFRACTIONf_dihedral_angle_d7.9252664
X-RAY DIFFRACTIONf_chiral_restr0.0552465
X-RAY DIFFRACTIONf_plane_restr0.0072643
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.8-1.820.25543020.21835741X-RAY DIFFRACTION96
1.82-1.840.24813110.20975896X-RAY DIFFRACTION100
1.84-1.860.23623120.19325932X-RAY DIFFRACTION100
1.86-1.890.24063140.18255963X-RAY DIFFRACTION100
1.89-1.910.20793120.1825934X-RAY DIFFRACTION100
1.91-1.940.22673130.17865937X-RAY DIFFRACTION100
1.94-1.970.2133110.17715922X-RAY DIFFRACTION100
1.97-20.22583130.1735940X-RAY DIFFRACTION100
2-2.030.2023120.16855924X-RAY DIFFRACTION100
2.03-2.060.20343130.15915949X-RAY DIFFRACTION100
2.06-2.10.18993110.16525922X-RAY DIFFRACTION100
2.1-2.130.22843140.16985959X-RAY DIFFRACTION100
2.13-2.170.1963140.16065972X-RAY DIFFRACTION100
2.17-2.220.20173120.15775923X-RAY DIFFRACTION100
2.22-2.270.1933140.15415970X-RAY DIFFRACTION100
2.27-2.320.19613150.15635983X-RAY DIFFRACTION100
2.32-2.380.19163120.15535930X-RAY DIFFRACTION100
2.38-2.440.18413140.15955956X-RAY DIFFRACTION100
2.44-2.510.21343140.16395968X-RAY DIFFRACTION100
2.51-2.60.2023130.15965945X-RAY DIFFRACTION100
2.6-2.690.19253150.15945986X-RAY DIFFRACTION100
2.69-2.80.21493140.16535968X-RAY DIFFRACTION100
2.8-2.920.19423130.16275942X-RAY DIFFRACTION99
2.92-3.080.20213160.16356001X-RAY DIFFRACTION99
3.08-3.270.19433130.16385960X-RAY DIFFRACTION99
3.27-3.520.1773190.1616044X-RAY DIFFRACTION99
3.52-3.880.15933160.1486018X-RAY DIFFRACTION99
3.88-4.440.16833170.14896019X-RAY DIFFRACTION99
4.44-5.590.18043200.15856075X-RAY DIFFRACTION99
5.59-40.940.21073310.20266272X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.9906-0.85570.11021.6221-0.28371.1527-0.0090.05630.1157-0.0806-0.0036-0.1471-0.05820.00650.00160.0209-0.04410.00720.11970.01350.148767.893228.203250.4031
21.3558-0.49510.62080.7088-0.29090.87-0.0040.10160.1571-0.0068-0.0359-0.1359-0.0690.06550.03710.1284-0.0150.01490.1280.01880.123152.082943.234941.8659
30.7266-0.6865-0.35921.2860.13641.0676-0.00710.00050.0747-0.02210.0073-0.063-0.05910.063-0.00350.0971-0.0219-0.01360.11490.02040.124266.107828.972552.229
41.53080.50750.87962.5695-0.79072.777-0.02830.2713-0.0668-0.3174-0.0314-0.42070.1310.50740.07080.13670.0190.03470.21680.02350.218679.080118.835847.7981
50.39270.09460.3650.49910.34171.1041-0.02310.0561-0.0341-0.07280.0257-0.10760.06040.18750.00190.14950.02220.03810.1855-0.01010.18134.471520.146-3.7751
63.26811.3931.76450.96660.87822.5649-0.13040.7809-0.3404-0.22340.2038-0.15280.02620.2506-0.0910.2508-0.00610.07460.2353-0.06090.198121.75112.5117-25.9114
71.24080.6057-0.02220.9565-0.13310.5008-0.03320.0824-0.1687-0.09370.0258-0.0420.06420.01770.00730.14620.00310.02860.1391-0.02450.10914.630510.3614-10.291
80.3912-0.07940.51320.9899-0.03790.7766-0.00930.0192-0.0065-0.06660.0087-0.07660.06260.06650.00490.12890.00870.03810.1454-0.01180.122331.475522.23311.7356
91.750.8274-0.42312.18961.01622.5541-0.0507-0.1108-0.3680.1120.0585-0.50640.3580.417-0.01230.1570.0620.00620.22980.00470.229344.310218.011611.8855
101.21840.86310.29991.93190.94881.2814-0.0508-0.05630.1869-0.0935-0.0480.3255-0.1606-0.16720.08410.09950.02190.00680.1847-0.01620.1455-1.486134.014211.1613
111.03260.49590.57420.74650.46640.8382-0.0417-0.05340.07260.0114-0.03010.0621-0.0563-0.04370.07210.12640.01180.01210.1324-0.00010.105613.829840.277916.8939
121.3194-0.31520.88582.02770.81481.4855-0.0263-0.2571-0.11110.2409-0.05740.40130.1446-0.37760.06880.1362-0.03830.05660.2192-0.0150.1985-8.663818.171913.6375
130.5607-0.19220.40010.978-0.68281.20470.0014-0.0749-0.07690.03980.08690.12240.058-0.2136-0.07390.0837-0.02160.00470.16430.02020.142233.555518.474664.9344
144.2501-2.63162.55641.6844-1.44892.5284-0.1576-0.4923-0.11870.15810.26960.0922-0.2207-0.2534-0.11250.16880.00460.02860.1690.02150.143646.679610.650785.8482
151.1858-0.68340.17890.9909-0.26190.63730.00620.0012-0.0548-0.0367-0.0035-0.01440.0402-0.03520.00080.1122-0.0177-0.00050.12520.00290.101253.93159.636870.0777
160.62120.0550.47051.06620.08571.32340.01110.0136-0.0064-0.01750.04190.0589-0.0127-0.0279-0.04950.1059-0.00090.00430.14270.02540.117236.321421.237559.0381
171.7174-0.8846-0.02561.4753-1.32853.0528-0.04860.1119-0.3823-0.33720.1280.38920.4234-0.3372-0.07230.1684-0.0649-0.0340.21360.01540.212225.499614.838747.3039
181.3405-1.0762-1.11191.6828-0.22232.4627-0.03550.0516-0.1932-0.1883-0.00240.58090.2425-0.45230.04740.1433-0.0405-0.04590.25140.05370.229921.689819.423549.7147
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'D' and (resid 17 through 60 )
2X-RAY DIFFRACTION2chain 'D' and (resid 61 through 300 )
3X-RAY DIFFRACTION3chain 'D' and (resid 301 through 390 )
4X-RAY DIFFRACTION4chain 'D' and (resid 391 through 462 )
5X-RAY DIFFRACTION5chain 'A' and (resid 17 through 86 )
6X-RAY DIFFRACTION6chain 'A' and (resid 87 through 127 )
7X-RAY DIFFRACTION7chain 'A' and (resid 128 through 300 )
8X-RAY DIFFRACTION8chain 'A' and (resid 301 through 390 )
9X-RAY DIFFRACTION9chain 'A' and (resid 391 through 462 )
10X-RAY DIFFRACTION10chain 'C' and (resid 17 through 86 )
11X-RAY DIFFRACTION11chain 'C' and (resid 87 through 365 )
12X-RAY DIFFRACTION12chain 'C' and (resid 366 through 462 )
13X-RAY DIFFRACTION13chain 'B' and (resid 17 through 86 )
14X-RAY DIFFRACTION14chain 'B' and (resid 87 through 127 )
15X-RAY DIFFRACTION15chain 'B' and (resid 128 through 300 )
16X-RAY DIFFRACTION16chain 'B' and (resid 301 through 390 )
17X-RAY DIFFRACTION17chain 'B' and (resid 391 through 428 )
18X-RAY DIFFRACTION18chain 'B' and (resid 429 through 462 )

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