登録情報 データベース : PDB / ID : 8i7o 構造の表示 ダウンロードとリンクタイトル In situ structure of axonemal doublet microtubules in mouse sperm with 16-nm repeat 要素(Cilia- and flagella-associated protein ...) x 3 (EF-hand domain-containing ...) x 2 Cilia and flagella-associated protein 77 Coiled-coil domain-containing protein 105 Detyrosinated tubulin alpha-3 chain Dual specificity phosphatase 21 EF-hand calcium-binding domain-containing protein 6 Enkurin Parkin coregulated gene protein homolog Protein FAM166A Protein FAM166C Protein Flattop Sperm acrosome-associated protein 9 Tektin bundle-interacting protein 1 Tektin-1 Tektin-2 Tektin-3 Tektin-4 Tektin-5 Testis-expressed protein 43 Tubulin beta-4B chain 残り17件を表示 表示を減らす 詳細キーワード STRUCTURAL PROTEIN (タンパク質) / microtubules (微小管) / axoneme / sperm / filament機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
male germ-line stem cell population maintenance / axonemal A tubule inner sheath / outer acrosomal membrane / axonemal B tubule inner sheath / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III ... male germ-line stem cell population maintenance / axonemal A tubule inner sheath / outer acrosomal membrane / axonemal B tubule inner sheath / regulation of brood size / establishment of left/right asymmetry / 9+0 motile cilium / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / Cilium Assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / Intraflagellar transport / inner dynein arm assembly / Carboxyterminal post-translational modifications of tubulin / protein polyglutamylation / cilium-dependent cell motility / positive regulation of feeding behavior / COPI-independent Golgi-to-ER retrograde traffic / regulation of cilium beat frequency involved in ciliary motility / sperm principal piece / MAP kinase tyrosine/serine/threonine phosphatase activity / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / cilium movement involved in cell motility / 9+2 motile cilium / PKR-mediated signaling / COPI-mediated anterograde transport / protein localization to organelle / Aggrephagy / acrosomal membrane / Kinesins / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / RHO GTPases activate IQGAPs / The role of GTSE1 in G2/M progression after G2 checkpoint / axoneme assembly / Recycling pathway of L1 / axonemal microtubule / cilium organization / flagellated sperm motility / COPI-dependent Golgi-to-ER retrograde traffic / RHO GTPases Activate Formins / Separation of Sister Chromatids / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition / manchette / MHC class II antigen presentation / protein targeting to mitochondrion / 繊毛 / positive regulation of cell motility / protein targeting to membrane / 微小管形成中心 / tubulin complex / regulation of neuron projection development / ciliary base / myosin phosphatase activity / extrinsic component of membrane / 細胞結合 / protein-serine/threonine phosphatase / beta-tubulin binding / regulation of cell division / cytoplasmic microtubule / phosphatase activity / axoneme / mitotic cytokinesis / cilium assembly / alpha-tubulin binding / spermatid development / sperm flagellum / cellular response to unfolded protein / microtubule-based process / 脱リン酸化 / sperm midpiece / Hsp70 protein binding / 中心小体 / Neutrophil degranulation / プロテインチロシンホスファターゼ / mitotic spindle organization / acrosomal vesicle / ciliary basal body / cellular response to leukemia inhibitory factor / protein tyrosine phosphatase activity / G protein-coupled receptor binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / Hsp90 protein binding / protein localization / ミトコンドリア / 繊毛 / structural constituent of cytoskeleton / 紡錘体 / microtubule cytoskeleton organization / SH3 domain binding / 紡錘体 / intracellular calcium ion homeostasis / calcium-dependent protein binding 類似検索 - 分子機能 Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Sperm-associated microtubule inner protein 10 / DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / EF-hand domain / Tektin bundle interacting protein 1 / Sperm acrosome-associated protein 9 / : ... Atypical dual specificity phosphatase, subfamily B / Protein of unknown function DUF4513 / Sperm-associated microtubule inner protein 10 / DJBP, EF-hand domain / Tektin bundle interacting protein 1 / Coiled-coil domain-containing protein 105 / EF-hand domain / Tektin bundle interacting protein 1 / Sperm acrosome-associated protein 9 / : / Sperm acrosome-associated protein 9 / Tektin / Cilia- and flagella-associated protein 276 / Tektin family / Protein of unknown function (DUF3695) / Cilia- and flagella-associated protein 77 / Cilia- and flagella-associated protein 77 / Uncharacterised protein FAM166/UPF0605 / Protein Flattop / Ciliary microtubule inner protein 2B-like / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Dual specificity phosphatase, catalytic domain / EFハンド / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Dual specificity protein phosphatase domain / Dual specificity protein phosphatase domain profile. / EF-hand domain pair / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / チューブリン / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tyrosine specific protein phosphatases active site. / Protein-tyrosine phosphatase, active site / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tyrosine-specific protein phosphatases domain / Tyrosine specific protein phosphatases domain profile. / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Protein-tyrosine phosphatase-like / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40リピート / WD40リピート / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily 類似検索 - ドメイン・相同性 グアノシン三リン酸 / Cilia and flagella associated protein 77 / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Tektin-4 / Cilia- and flagella-associated protein 52 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop ... グアノシン三リン酸 / Cilia and flagella associated protein 77 / Tektin bundle-interacting protein 1 / Tektin-5 / Tubulin alpha-3 chain / Tubulin beta-4B chain / Tektin-4 / Cilia- and flagella-associated protein 52 / EF-hand calcium-binding domain-containing protein 6 / Protein Flattop / Enkurin / Tektin-3 / Sperm acrosome-associated protein 9 / Cilia- and flagella-associated protein 20 / Tektin-2 / EF-hand domain-containing family member C2 / Ciliary microtubule inner protein 2A / Tektin-like protein 1 / Dual specificity phosphatase 21 / Sperm-associated microtubule inner protein 10 / EF-hand domain-containing protein 1 / Cilia- and flagella-associated protein 276 / Tektin-1 / Parkin coregulated gene protein homolog / Ciliary microtubule inner protein 2C 類似検索 - 構成要素生物種 Mus musculus (ハツカネズミ)手法 電子顕微鏡法 / サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度 : 4.5 Å 詳細データ登録者 Zhu, Y. / Yin, G.L. / Tai, L.H. / Sun, F. 資金援助 中国, 1件 詳細 詳細を隠す組織 認可番号 国 National Natural Science Foundation of China (NSFC) 31925026 中国
引用ジャーナル : Cell Discov / 年 : 2023タイトル : In-cell structural insight into the stability of sperm microtubule doublet.著者 : Linhua Tai / Guoliang Yin / Xiaojun Huang / Fei Sun / Yun Zhu / 要旨 : The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. ... The propulsion for mammalian sperm swimming is generated by flagella beating. Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential structural blocks of flagella. However, the intricate molecular architecture of intact sperm DMT remains elusive. Here, by in situ cryo-electron tomography, we solved the in-cell structure of mouse sperm DMT at 4.5-7.5 Å resolutions, and built its model with 36 kinds of MIPs in 48 nm periodicity. We identified multiple copies of Tektin5 that reinforce Tektin bundle, and multiple MIPs with different periodicities that anchor the Tektin bundle to tubulin wall. This architecture contributes to a superior stability of A-tubule than B-tubule of DMT, which was revealed by structural comparison of DMTs from the intact and deformed axonemes. Our work provides an overall molecular picture of intact sperm DMT in 48 nm periodicity that is essential to understand the molecular mechanism of sperm motility as well as the related ciliopathies. 履歴 登録 2023年2月1日 登録サイト : PDBJ / 処理サイト : PDBJ改定 1.0 2023年10月11日 Provider : repository / タイプ : Initial release改定 1.1 2023年12月6日 Group : Database references / カテゴリ : citation / citation_authorItem : _citation.journal_volume / _citation.page_first ... _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
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