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Open data
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Basic information
Entry | Database: PDB / ID: 8hpy | ||||||
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Title | Crystal structure of human LGI1-ADAM22 complex | ||||||
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Function / homology | ![]() LGI-ADAM interactions / axon initial segment / negative regulation of cell adhesion / neurotransmitter receptor localization to postsynaptic specialization membrane / positive regulation of synaptic transmission / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, H. / Xu, F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of human LGI1-ADAM22 complex Authors: Liu, H. / Xu, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 414.2 KB | Display | ![]() |
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PDB format | ![]() | 331.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5zxr S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53743.102 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 60616.598 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Sugar | ChemComp-NAG / ![]() #4: Chemical | ChemComp-CA / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.02 Å3/Da / Density % sol: 69.42 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 2.5M sodium chloride, 0.1M HEPES pH=7.5, 2% (w/v) Benzamidine |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 17, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 5.87→50 Å / Num. obs: 9884 / % possible obs: 99.1 % / Redundancy: 6.9 % / Rmerge(I) obs: 0.073 / Rsym value: 0.058 / Net I/σ(I): 25.905 |
Reflection shell | Resolution: 5.87→5.97 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.891 / Mean I/σ(I) obs: 2.514 / Num. unique obs: 502 / Rsym value: 0.625 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5ZXR ![]() 5zxr Resolution: 5.87→48.08 Å / SU ML: 1.3 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 46.57
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 5.87→48.08 Å
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Refine LS restraints |
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LS refinement shell |
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