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- PDB-8hep: Solution structure of the periplasmic domain of the anti-sigma fa... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8hep | ||||||
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Title | Solution structure of the periplasmic domain of the anti-sigma factor RsgI1 from Clostridium thermocellum | ||||||
![]() | (Anti-sigma factor![]() | ||||||
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Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chen, C. / Feng, Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Essential autoproteolysis of bacterial anti-sigma factor RsgI for transmembrane signal transduction. Authors: Chen, C. / Dong, S. / Yu, Z. / Qiao, Y. / Li, J. / Ding, X. / Li, R. / Lin, J. / Bayer, E.A. / Liu, Y.J. / Cui, Q. / Feng, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 923.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8hdjC ![]() 8heqC ![]() 8herC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | ![]() Mass: 1190.390 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rsgI1 / Production host: ![]() ![]() ![]() |
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#2: Protein | ![]() Mass: 19081.623 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rsgI1 / Production host: ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 50 mM sodium phosphate, 150 mM sodium chloride, 1 mM [U-100% 13C; U-100% 15N] RsgI1-PD, 0.02 % w/v DSS, 100% D2O Label: sample1 / Solvent system: 100% D2O | ||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 200 mM / Label: condition1 / pH: 6.8 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model![]() |
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Processing
NMR software |
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Refinement | Method: ![]() | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |