PDB-8glv: 96-nm repeat unit of doublet microtubules from Chlamydomonas rein...

Basic information

• Entry: Database: PDB / ID: 8glv
• Title: 96-nm repeat unit of doublet microtubules from Chlamydomonas reinhardtii flagella

Components

• (AAA+ ATPase domain-containing ...) x 5
• (Axonemal inner arm ...) x 2
• (Cilia- and flagella-associated protein ...) x 9
• (DUF4201 domain-containing ...) x 2
• (Dynein light chain ...) x 5
• (Dynein regulatory complex protein ...) x 4
• (Dynein regulatory complex subunit ...) x 4
• (Flagellar associated ...) x 19
• (Flagellar outer dynein arm ...) x 2
• (Flagellar radial spoke protein ...) x 4
• (Flagellar-associated protein ...) x 2
• (Nucleoside diphosphate ...Nucleotide) x 2
• (Outer dynein arm-docking complex ...) x 2
• (Radial spoke protein ...) x 9
• (Subunit of axonemal inner ...) x 2
• (UNKNOWN) x 2
• (WD_REPEATS_REGION domain-containing ...) x 2
• 28 kDa inner dynein arm light chain, axonemal
• ATPase AAA-type core domain-containing protein
• Actin
• CFAP91 domain-containing protein
• Calmodulin
• CaltractinCentrin
• Coiled-coil protein associated with protofilament ribbonsCoiled coil
• Cytochrome b5 heme-binding domain-containing protein
• DHC2
• DHC_N2 domain-containing protein
• DRC5
• Dynein 11 kDa light chain, flagellar outer arm
• Dynein 14 kDa light chain, flagellar outer arm
• Dynein 16 kDa light chain, flagellar outer arm
• Dynein 18 kDa light chain, flagellar outer arm
• Dynein 8 kDa light chain, flagellar outer arm
• Dynein axonemal light chain 1
• Dynein gamma chain, flagellar outer arm
• Dynein, 78 kDa intermediate chain, flagellar outer arm
• Dynein-1-beta heavy chain, flagellar inner arm I1 complex
• FAP1
• FAP129
• FAP143
• FAP166
• FAP207
• FAP21
• FAP210
• FAP251
• FAP253
• FAP306
• FAP339
• FAP34
• FAP385
• FAP43
• FAP44
• FAP52
• Heavy chain alpha
• IC140
• IC97/Casc1 N-terminal domain-containing protein
• Inner arm dynein light chain
• Inner-arm dynein f/I1 light chain
• Meiosis-specific nuclear structural protein 1
• Outer dynein arm protein 1
• PACRG
• Peptidyl-prolyl cis-trans isomeraseProlyl isomerase
• Protein Flattop homolog
• Protofilament ribbon protein of flagellar microtubules
• Radial spike protein 8
• Tubulin alpha
• Tubulin beta

• Keywords: MOTOR PROTEIN / cilia / microtubule / dynein

Function / homology

Function:

axonemal central pair / inner dynein arm / axonemal outer doublet / radial spoke head / outer dynein arm / axonemal dynein complex / axonemal dynein complex assembly / radial spoke / intraciliary transport particle B / outer dynein arm assembly / inner dynein arm assembly / cilium-dependent cell motility / membrane-bounded organelle / positive regulation of cilium-dependent cell motility / cellular anatomical entity / regulation of cilium beat frequency involved in ciliary motility / dynein light chain binding / kinocilium / intraciliary transport / cilium movement involved in cell motility / establishment of protein localization to organelle / 9+2 motile cilium / cilium movement / dynein heavy chain binding / axoneme assembly / motile cilium assembly / cilium organization / axonemal microtubule / intracellular organelle / blue light photoreceptor activity / cell projection organization / negative regulation of microtubule depolymerization / dynein complex / nucleoside-diphosphate kinase / misfolded protein binding / minus-end-directed microtubule motor activity / cytoplasmic dynein complex / myosin II complex / dynein light intermediate chain binding / Set1C/COMPASS complex / UTP biosynthetic process / ciliary plasm / CTP biosynthetic process / Oxidoreductases / motile cilium / GTP biosynthetic process / microtubule associated complex / nucleoside diphosphate kinase activity / microtubule motor activity / ciliary base / dynein intermediate chain binding / dynein complex binding / beta-tubulin binding / microtubule-based movement / axoneme / chaperone cofactor-dependent protein refolding / alpha-tubulin binding / mitotic cytokinesis / cilium assembly / cellular response to unfolded protein / enzyme regulator activity / microtubule-based process / protein peptidyl-prolyl isomerization / regulation of microtubule cytoskeleton organization / protein folding chaperone / Hsp70 protein binding / heat shock protein binding / centriole / FAD binding / chloroplast / GTPase activator activity / mitotic spindle organization / acrosomal vesicle / ciliary basal body / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / cell projection / cell motility / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / Hsp90 protein binding / cilium / structural constituent of cytoskeleton / mitotic spindle / small GTPase binding / unfolded protein binding / protein folding / mitotic cell cycle / protein-folding chaperone binding / protein refolding / microtubule binding / microtubule / oxidoreductase activity / cytoskeleton / calmodulin binding / hydrolase activity / cell cycle / cell division / phosphorylation / GTPase activity

Domain/homology:

: / Dynein-f, intermediate chain IC97/Dnai7-like / Domain of unknown function DUF4200 / Domain of unknown function DUF4201 / Protein of unknown function DUF4464 / IC97/Casc1, N-terminal / Cilia- and flagella-associated protein 61, N-terminal domain / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 61 / Domain of unknown function (DUF4200) / Domain of unknown function (DUF4201) / Domain of unknown function (DUF4464) / Cancer susceptibility candidate 1 N-terminus / Cilia- and flagella-associated protein 61, N-terminal domain / Axonemal dynein light chain / Dynein regulatory complex protein 1, C-terminal / Nucleoside diphosphate kinase 6 / Axonemal dynein light chain / Sperm tail C-terminal domain / TRAF3-interacting protein 1 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Radial spoke protein 14 / : / Domain of unknown function (DUF6872) / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / Cilia- and flagella-associated protein 91 / Stabilizer of axonemal microtubules 1/2 / IQ and ubiquitin-like domain-containing protein / Growth arrest-specific protein 8 domain / FAM183 family / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / Growth-arrest specific micro-tubule binding / Sperm tail / FAM183A and FAM183B related / Radial spokehead-like protein / Radial spoke 3 / Kelch repeat type 2 / Radial spokehead-like protein / Radial spoke protein 3 / Cilia- and flagella-associated protein 77 / Kelch motif / Cilia- and flagella-associated protein 77 / : / Sdc1/DPY30 / Nucleoside diphosphate kinase 7 / Meiosis-specific nuclear structural protein 1 / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Protein Flattop / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Galactose oxidase, central domain / Trichohyalin-plectin-homology domain / Piercer of microtubule wall 1/2 / Cilia- and flagella-associated protein 141 / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / Calmodulin-binding / Enkurin domain profile. / Dpy-30 motif / Dpy-30 motif / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Galactose oxidase, central domain / Dynein light chain roadblock-type 1/2 / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / BLUF domain / Sensors of blue-light using FAD / Tetratricopeptide repeat / Phospholipase D/Transphosphatidylase / Phospholipase D phosphodiesterase active site profile. / Dynein light chain, type 1/2, conserved site / Dynein light chain type 1 signature. / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / HSP40/DnaJ peptide-binding / Chaperone DnaJ, C-terminal / DnaJ C terminal domain / Parkin co-regulated protein

Component:

GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Flagellar-associated protein 172 / Flagellar-associated protein 59 / Flagella associated protein / DUF4201 domain-containing protein / Meiosis-specific nuclear structural protein 1 / AAA+ ATPase domain-containing protein / Uncharacterized protein / Flagellar associated protein / AAA+ ATPase domain-containing protein / IC97/Casc1 N-terminal domain-containing protein / AAA+ ATPase domain-containing protein / Cilia- and flagella-associated protein 52 / IQ and ubiquitin-like domain-containing protein / Flagellar associated protein / Uncharacterized protein / Cilia- and flagella-associated protein 299 / Cilia- and flagella-associated protein 44 / Cytochrome b5 heme-binding domain-containing protein / Uncharacterized protein / Flagellar associated protein / ATPase AAA-type core domain-containing protein / MORN repeat-containing protein 3 / Cilia- and flagella-associated protein 91 / EF-hand domain-containing protein / AAA+ ATPase domain-containing protein / Uncharacterized protein / Flagellar associated protein / PLD phosphodiesterase domain-containing protein / DUF4201 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / TPH domain-containing protein / Uncharacterized protein / AAA+ ATPase domain-containing protein / Uncharacterized protein / Uncharacterized protein / Dynein regulatory complex protein 9 / Inner-arm dynein f/I1 light chain / Flagellar associated protein / Flagellar associated protein / Subunit of axonemal inner dynein / Radial spoke protein 14 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Uncharacterized protein / Flagellar associated protein / Axonemal inner arm dynein I1/f subunit / Peptidyl-prolyl cis-trans isomerase / Cilia- and flagella-associated protein 100 / Radial spoke protein 4 / Cilia- and flagella-associated protein 45 / Cilia- and flagella-associated protein 77 / Flagellar associated protein / Calmodulin / Cilia- and flagella-associated protein 61 / Uncharacterized protein / Radial spoke protein 16 / Uncharacterized protein / Cilia- and flagella-associated protein 53 / Cilia- and flagella-associated protein 20 / Protein Flattop homolog / Dynein regulatory complex subunit 3 / Flagellar associated protein / Predicted protein / Dynein light chain roadblock / Uncharacterized protein / Dynein regulatory complex protein 10 / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Dynein regulatory complex protein 8 / Uncharacterized protein / Dynein light chain / Uncharacterized protein / Dynein regulatory complex subunit 2 / Flagellar associated protein / Flagellar associated protein / Predicted protein / Flagellar associated protein / Outer dynein arm protein 1 / Flagellar associated protein / Nucleoside diphosphate kinase / Dynein regulatory complex subunit 6 / Flagellar associated protein / Subunit of axonemal inner dynein / Parkin-co-regulated gene product / Cilia- and flagella-associated protein 73 / Mr19,000 outer arm dynein light chain / Dynein light chain Tctex1 / Tubulin beta-1/beta-2 chain / Caltractin / Tubulin alpha-1 chain / Dynein regulatory complex protein 1 / Actin

• Biological species: Chlamydomonas reinhardtii (plant)
• Method: ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å
• Authors: Walton, T. / Brown, A.

Funding support

United States, 2items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM141109	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	GM143183	United States

• Citation: Journal: Nature / Year: 2023; Title: Axonemal structures reveal mechanoregulatory and disease mechanisms.; Authors: Travis Walton / Miao Gui / Simona Velkova / Mahmoud R Fassad / Robert A Hirst / Eric Haarman / Christopher O'Callaghan / Mathieu Bottier / Thomas Burgoyne / Hannah M Mitchison / Alan Brown / ; Abstract: Motile cilia and flagella beat rhythmically on the surface of cells to power the flow of fluid and to enable spermatozoa and unicellular eukaryotes to swim. In humans, defective ciliary motility can lead to male infertility and a congenital disorder called primary ciliary dyskinesia (PCD), in which impaired clearance of mucus by the cilia causes chronic respiratory infections. Ciliary movement is generated by the axoneme, a molecular machine consisting of microtubules, ATP-powered dynein motors and regulatory complexes. The size and complexity of the axoneme has so far prevented the development of an atomic model, hindering efforts to understand how it functions. Here we capitalize on recent developments in artificial intelligence-enabled structure prediction and cryo-electron microscopy (cryo-EM) to determine the structure of the 96-nm modular repeats of axonemes from the flagella of the alga Chlamydomonas reinhardtii and human respiratory cilia. Our atomic models provide insights into the conservation and specialization of axonemes, the interconnectivity between dyneins and their regulators, and the mechanisms that maintain axonemal periodicity. Correlated conformational changes in mechanoregulatory complexes with their associated axonemal dynein motors provide a mechanism for the long-hypothesized mechanotransduction pathway to regulate ciliary motility. Structures of respiratory-cilia doublet microtubules from four individuals with PCD reveal how the loss of individual docking factors can selectively eradicate periodically repeating structures.

History

Deposition	Mar 23, 2023	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	May 31, 2023	Provider: repository / Type: Initial release
Revision 1.1	Jun 14, 2023	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2	Jun 28, 2023	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID

Assembly

Deposited unit

0A: Tubulin beta

0B: Tubulin alpha

0C: Tubulin beta

0E: Tubulin beta

0F: Tubulin alpha

0G: Tubulin beta

0H: Tubulin alpha

0I: Tubulin beta

0J: Tubulin alpha

0K: Tubulin beta

0L: Tubulin alpha

0M: Tubulin beta

0N: Tubulin alpha

0O: Tubulin beta

0P: Tubulin alpha

0Q: Tubulin beta

0R: Tubulin alpha

0S: Tubulin beta

0U: Tubulin beta

0V: Tubulin alpha

0W: Tubulin beta

0X: Tubulin alpha

0Y: Tubulin beta

0Z: Tubulin alpha

1A: Tubulin beta

1B: Tubulin alpha

1C: Tubulin beta

1D: Tubulin alpha

1E: Tubulin beta

1F: Tubulin alpha

1G: Tubulin beta

1H: Tubulin alpha

1K: Tubulin beta

1L: Tubulin alpha

1M: Tubulin beta

1N: Tubulin alpha

1O: Tubulin beta

1P: Tubulin alpha

1Q: Tubulin beta

1R: Tubulin alpha

1S: Tubulin beta

1T: Tubulin alpha

1U: Tubulin beta

1V: Tubulin alpha

1W: Tubulin beta

1X: Tubulin alpha

1Z: Tubulin alpha

2A: Tubulin beta

2B: Tubulin alpha

2C: Tubulin beta

2D: Tubulin alpha

2E: Tubulin beta

2F: Tubulin alpha

2G: Tubulin beta

2H: Tubulin alpha

2I: Tubulin beta

2J: Tubulin alpha

2K: Tubulin beta

2L: Tubulin alpha

2M: Tubulin beta

2N: Tubulin alpha

2P: Tubulin alpha

2Q: Tubulin beta

2R: Tubulin alpha

2S: Tubulin beta

2T: Tubulin alpha

2U: Tubulin beta

2V: Tubulin alpha

2W: Tubulin beta

2X: Tubulin alpha

2Y: Tubulin beta

2Z: Tubulin alpha

3A: Tubulin beta

3B: Tubulin alpha

3C: Tubulin beta

3D: Tubulin alpha

3F: Tubulin alpha

3G: Tubulin beta

3H: Tubulin alpha

3I: Tubulin beta

3J: Tubulin alpha

3K: Tubulin beta

3L: Tubulin alpha

3M: Tubulin beta

3N: Tubulin alpha

3O: Tubulin beta

3P: Tubulin alpha

3Q: Tubulin beta

3R: Tubulin alpha

3S: Tubulin beta

3T: Tubulin alpha

3V: PACRG

3W: Cilia- and flagella-associated protein 20

3X: PACRG

3Y: Cilia- and flagella-associated protein 20

3Z: PACRG

4A: Cilia- and flagella-associated protein 20

4B: PACRG

4C: Cilia- and flagella-associated protein 20

4D: PACRG

4E: Cilia- and flagella-associated protein 20

4F: PACRG

4G: Cilia- and flagella-associated protein 20

4H: PACRG

4I: Cilia- and flagella-associated protein 20

4J: PACRG

4O: Meiosis-specific nuclear structural protein 1

4Q: Flagellar associated protein

4R: Coiled-coil protein associated with protofilament ribbons

4W: Flagellar associated protein

4X: Cilia- and flagella-associated protein 45

5C: Cilia- and flagella-associated protein 45

5D: Meiosis-specific nuclear structural protein 1

5E: FAP34

5F: Flagellar associated protein

5H: Protein Flattop homolog

5I: Protein Flattop homolog

5J: Protein Flattop homolog

5K: Protein Flattop homolog

5L: FAP52

5M: FAP52

5N: FAP52

5O: Nucleoside diphosphate kinase

5P: Nucleoside diphosphate kinase

5Q: Flagellar associated protein

5S: Flagellar associated protein

5T: Flagellar associated protein

5U: Flagellar associated protein

5V: Flagellar associated protein

5X: Flagellar associated protein

5Y: Flagellar associated protein

5Z: Flagellar associated protein

6A: Flagellar associated protein

6B: Flagellar associated protein

6C: Flagellar associated protein

6D: Flagellar associated protein

6E: FAP143

6F: Flagellar associated protein

6G: FAP166

6H: FAP166

6I: Flagellar associated protein

6J: Flagellar associated protein

6K: Flagellar associated protein

6L: Flagellar associated protein

6M: Flagellar associated protein

6N: Flagellar associated protein

6O: Flagellar associated protein

6P: Flagellar associated protein

6Q: FAP339

6R: FAP339

6S: FAP339

6T: Protofilament ribbon protein of flagellar microtubules

6U: Protofilament ribbon protein of flagellar microtubules

6V: Protofilament ribbon protein of flagellar microtubules

6W: Protofilament ribbon protein of flagellar microtubules

6X: Protofilament ribbon protein of flagellar microtubules

6Y: Protofilament ribbon protein of flagellar microtubules

6Z: Protofilament ribbon protein of flagellar microtubules

7B: Flagellar associated protein

7C: Flagellar associated protein

7D: Flagellar associated protein

7E: Flagellar associated protein

7G: Flagellar associated protein

7H: Flagellar associated protein

7I: FAP129

7J: FAP129

7K: FAP21

7M: Flagellar associated protein

7N: Flagellar associated protein

7P: Flagellar associated protein

7Q: FAP306

7X: Flagellar associated protein

7Y: Flagellar associated protein

8B: Flagellar associated protein

8C: Flagellar associated protein

8F: Tubulin alpha

8H: Tubulin beta

8I: Tubulin alpha

8J: Tubulin beta

8K: Tubulin alpha

8L: Tubulin beta

8M: Tubulin alpha

8N: Tubulin beta

A0: Tubulin alpha

A1: Tubulin beta

A2: Tubulin alpha

A3: Tubulin beta

A4: Tubulin alpha

A5: Tubulin beta

A6: Tubulin beta

A7: Tubulin beta

A8: Tubulin alpha

A9: Tubulin beta

AA: Dynein axonemal light chain 1

AB: Outer dynein arm-docking complex subunit 1

AC: Outer dynein arm-docking complex protein DC3

AD: Dynein gamma chain, flagellar outer arm

AE: IC97/Casc1 N-terminal domain-containing protein

AF: Axonemal inner arm I1 intermediate chain dynein IC138

AG: IC140

AH: Axonemal inner arm dynein I1/f subunit

AI: Dynein light chain roadblock LC7b

AJ: Tubulin beta

AK: Dynein light chain roadblock LC7a

AL: Dynein 8 kDa light chain, flagellar outer arm

AM: Dynein 8 kDa light chain, flagellar outer arm

AN: Dynein 8 kDa light chain, flagellar outer arm

AO: Dynein 8 kDa light chain, flagellar outer arm

AP: Dynein 8 kDa light chain, flagellar outer arm

AQ: Dynein 8 kDa light chain, flagellar outer arm

AR: Dynein light chain Tctex1

AS: Inner arm dynein light chain

AT: Tubulin alpha

AU: Dynein light chain 10

AV: Cilia- and flagella-associated protein 73

AW: Cilia- and flagella-associated protein 100

AX: Dynein gamma chain, flagellar outer arm

AY: Outer dynein arm-docking complex subunit 1

AZ: Outer dynein arm-docking complex protein DC3

Aa: Dynein, 78 kDa intermediate chain, flagellar outer arm

Ab: Dynein, 78 kDa intermediate chain, flagellar outer arm

Ac: WD_REPEATS_REGION domain-containing protein

Ad: FAP52

Ae: FAP52

Af: FAP52

Ag: Tubulin alpha

Ah: Tubulin beta

Ai: Tubulin alpha

Aj: Tubulin beta

Ak: Tubulin alpha

Al: Tubulin beta

Am: Tubulin alpha

An: Tubulin beta

Ao: Tubulin alpha

Ap: Tubulin beta

Aq: WD_REPEATS_REGION domain-containing protein

Ar: Flagellar outer dynein arm light chain 2

As: Flagellar outer dynein arm light chain 2

At: Tubulin alpha

Au: Tubulin beta

Av: Tubulin alpha

Aw: Tubulin beta

Ax: Tubulin alpha

Ay: Tubulin beta

Az: Tubulin alpha

B0: Tubulin alpha

B1: Tubulin beta

B2: Tubulin alpha

B3: Tubulin beta

B4: Tubulin alpha

B5: Tubulin beta

B6: Tubulin beta

B7: Tubulin alpha

B8: Tubulin beta

B9: Tubulin alpha

BA: Tubulin alpha

BB: Tubulin beta

BC: Dynein 18 kDa light chain, flagellar outer arm

BD: Dynein 18 kDa light chain, flagellar outer arm

BE: Dynein 11 kDa light chain, flagellar outer arm

BF: Tubulin alpha

BG: Tubulin beta

BH: Tubulin alpha

BI: Tubulin beta

BJ: Tubulin alpha

BK: Tubulin beta

BL: Tubulin alpha

BM: Tubulin beta

BN: Tubulin alpha

BO: Tubulin beta

BP: Dynein 11 kDa light chain, flagellar outer arm

BQ: Dynein light chain roadblock LC7a

BR: Dynein light chain roadblock LC7a

BS: Tubulin beta

BT: Tubulin alpha

BU: Tubulin beta

BV: Tubulin alpha

BW: Tubulin beta

BX: Tubulin alpha

BY: Tubulin beta

BZ: Tubulin alpha

Ba: Tubulin beta

Bb: Tubulin alpha

Bc: Dynein light chain roadblock LC7b

Bd: Dynein light chain roadblock LC7b

Be: Dynein 8 kDa light chain, flagellar outer arm

Bf: Tubulin beta

Bg: Tubulin alpha

Bh: Tubulin beta

Bi: Tubulin alpha

Bj: Tubulin beta

Bk: Tubulin alpha

Bl: Tubulin beta

Bm: Tubulin alpha

Bn: Tubulin beta

Bo: Tubulin alpha

Bp: Dynein 8 kDa light chain, flagellar outer arm

Bq: Dynein 8 kDa light chain, flagellar outer arm

Br: Dynein 8 kDa light chain, flagellar outer arm

Bs: Tubulin beta

Bt: Tubulin alpha

Bu: Tubulin beta

Bv: Tubulin alpha

Bw: Tubulin beta

Bx: Tubulin alpha

By: Tubulin beta

Bz: Tubulin alpha

C0: Tubulin beta

C1: Tubulin alpha

C2: Tubulin beta

C3: Tubulin alpha

C4: Tubulin beta

C5: Tubulin alpha

C6: Tubulin beta

C7: Tubulin alpha

C8: Tubulin beta

C9: Tubulin alpha

CA: Tubulin alpha

CB: Dynein 8 kDa light chain, flagellar outer arm

CC: Dynein 8 kDa light chain, flagellar outer arm

CD: Dynein 8 kDa light chain, flagellar outer arm

CE: Tubulin beta

CF: Tubulin alpha

CG: Tubulin beta

CH: Tubulin alpha

CI: Tubulin beta

CJ: Tubulin alpha

CK: Tubulin beta

CL: Tubulin alpha

CM: Tubulin beta

CN: Tubulin alpha

CO: Dynein 8 kDa light chain, flagellar outer arm

CP: Dynein light chain 9

CQ: Dynein light chain 9

CR: Tubulin beta

CS: Tubulin alpha

CT: Tubulin beta

CU: Tubulin alpha

CV: Tubulin beta

CW: Tubulin alpha

CX: Tubulin beta

CY: Tubulin alpha

CZ: Tubulin beta

Ca: Dynein light chain 10

Cb: Dynein axonemal light chain 1

Cc: Outer dynein arm protein 1

Cd: Outer dynein arm protein 1

Ce: Tubulin beta

Cf: Tubulin alpha

Cg: Tubulin beta

Ch: Tubulin alpha

Ci: Tubulin beta

Cj: Tubulin alpha

Ck: Tubulin beta

Cl: Tubulin alpha

Cm: Tubulin beta

Cn: AAA+ ATPase domain-containing protein

Co: Dynein-1-beta heavy chain, flagellar inner arm I1 complex

Cp: WD_REPEATS_REGION domain-containing protein

Cq: WD_REPEATS_REGION domain-containing protein

Cr: Tubulin beta

Cs: Tubulin alpha

Ct: Tubulin beta

Cu: Tubulin alpha

Cv: Tubulin beta

Cw: Tubulin alpha

Cx: Tubulin beta

Cy: Tubulin alpha

Cz: Tubulin beta

D0: Tubulin beta

D1: Tubulin alpha

D2: Tubulin beta

D3: Tubulin alpha

D4: Tubulin beta

D5: Tubulin alpha

D6: Tubulin beta

D7: Tubulin alpha

D8: Tubulin beta

D9: Inner-arm dynein f/I1 light chain

DA: Heavy chain alpha

DB: Flagellar outer dynein arm heavy chain beta

DC: Dynein 14 kDa light chain, flagellar outer arm

DD: Tubulin alpha

DE: Tubulin beta

DF: Tubulin alpha

DG: Tubulin beta

DH: Tubulin alpha

DI: Tubulin beta

DJ: Tubulin alpha

DK: Tubulin beta

DL: Tubulin alpha

DM: Tubulin beta

DN: Tubulin alpha

DO: Dynein 16 kDa light chain, flagellar outer arm

DP: Heavy chain alpha

DQ: Tubulin alpha

DR: Tubulin beta

DS: Tubulin alpha

DT: Tubulin beta

DU: Tubulin alpha

DV: Tubulin beta

DW: Tubulin alpha

DX: Tubulin beta

DY: Tubulin alpha

DZ: Tubulin beta

Da: Tubulin alpha

Db: Flagellar outer dynein arm heavy chain beta

Dc: Dynein 14 kDa light chain, flagellar outer arm

Dd: Tubulin alpha

De: Tubulin beta

Df: Tubulin alpha

Dg: Tubulin beta

Dh: Tubulin alpha

Di: Tubulin beta

Dj: Tubulin alpha

Dk: Tubulin beta

Dl: Tubulin alpha

Dm: Tubulin beta

Dn: Dynein 16 kDa light chain, flagellar outer arm

Do: FAP44

Dp: FAP43

Dq: Tubulin alpha

Dr: Tubulin beta

Ds: Tubulin alpha

Dt: Tubulin beta

Du: Tubulin alpha

Dv: Tubulin beta

Dw: Tubulin alpha

Dx: Tubulin beta

Dy: Tubulin alpha

Dz: Tubulin beta

E0: Tubulin beta

E1: Tubulin alpha

E2: Tubulin beta

E3: Tubulin alpha

E4: Tubulin beta

E5: Tubulin alpha

E6: Tubulin beta

E7: Tubulin alpha

E8: Tubulin beta

E9: Tubulin alpha

EA: Dynein regulatory complex protein 1

EB: Dynein regulatory complex subunit 2

EC: Tubulin alpha

ED: Tubulin beta

EE: Tubulin alpha

EF: Tubulin beta

EG: Tubulin alpha

EH: Tubulin beta

EI: Tubulin alpha

EJ: Tubulin beta

EK: Tubulin alpha

EL: Tubulin beta

EM: Dynein regulatory complex subunit 6

EN: Dynein regulatory complex subunit 4

EO: Dynein regulatory complex subunit 4

EP: Tubulin alpha

EQ: Tubulin beta

ER: Tubulin alpha

ES: Tubulin beta

ET: Tubulin alpha

EU: Tubulin beta

EV: Tubulin alpha

EW: Tubulin beta

EX: Tubulin alpha

EY: Tubulin beta

EZ: Dynein regulatory complex protein 8

Ea: ATPase AAA-type core domain-containing protein

Eb: Flagellar associated protein

Ec: Tubulin alpha

Ed: Tubulin beta

Ee: Tubulin alpha

Ef: Tubulin beta

Eg: Tubulin alpha

Eh: Tubulin beta

Ei: Tubulin alpha

Ej: Tubulin beta

Ek: Tubulin alpha

El: Tubulin beta

Em: Dynein regulatory complex subunit 3

En: Dynein regulatory complex protein 9

Eo: FAP52

Ep: Tubulin alpha

Eq: Tubulin beta

Er: Tubulin alpha

Es: Tubulin beta

Et: Tubulin alpha

Eu: Tubulin beta

Ev: Tubulin alpha

Ew: Tubulin beta

Ex: Tubulin alpha

Ey: Dynein regulatory complex protein 10

Ez: DRC5

F0: Tubulin beta

F1: Tubulin alpha

F2: Tubulin beta

F3: Tubulin alpha

F4: DUF4201 domain-containing protein

F5: Tubulin alpha

F6: Tubulin beta

F7: Tubulin alpha

F8: Tubulin beta

F9: Tubulin alpha

FA: Dynein 8 kDa light chain, flagellar outer arm

FB: Dynein 8 kDa light chain, flagellar outer arm

FC: Tubulin beta

FD: Tubulin alpha

FE: Tubulin beta

FF: Tubulin alpha

FG: Tubulin beta

FH: Tubulin alpha

FI: Tubulin beta

FJ: Tubulin alpha

FK: Tubulin beta

FL: Tubulin alpha

FM: Dynein light chain 9

FN: Dynein light chain 10

FO: Calmodulin

FP: Tubulin beta

FQ: Tubulin alpha

FR: Tubulin beta

FS: Tubulin alpha

FT: Tubulin beta

FU: Tubulin alpha

FV: Tubulin beta

FW: Tubulin alpha

FX: Tubulin beta

FY: Tubulin alpha

FZ: Caltractin

Fa: Dynein gamma chain, flagellar outer arm

Fb: Outer dynein arm-docking complex subunit 1

Fc: Tubulin beta

Fd: Tubulin alpha

Fe: Tubulin beta

Ff: Tubulin alpha

Fg: Tubulin beta

Fh: Tubulin alpha

Fi: Tubulin beta

Fj: Tubulin alpha

Fk: Tubulin beta

Fl: Tubulin alpha

Fm: AAA+ ATPase domain-containing protein

Fn: Outer dynein arm-docking complex protein DC3

Fo: Dynein, 78 kDa intermediate chain, flagellar outer arm

Fp: Tubulin beta

Fq: Tubulin alpha

Fr: Tubulin beta

Fs: Tubulin alpha

Ft: Tubulin beta

Fu: Tubulin alpha

Fv: Tubulin beta

Fw: Tubulin alpha

Fx: Tubulin beta

Fy: DUF4201 domain-containing protein

Fz: WD_REPEATS_REGION domain-containing protein

G0: Tubulin beta

G1: Tubulin alpha

G2: Tubulin beta

G3: Tubulin alpha

G4: Tubulin beta

G5: Tubulin alpha

G6: Tubulin beta

G7: Tubulin alpha

G8: Tubulin beta

G9: Tubulin alpha

GA: Flagellar outer dynein arm light chain 2

GB: Tubulin beta

GC: Tubulin alpha

GD: Tubulin beta

GE: Tubulin alpha

GF: Tubulin beta

GG: Tubulin alpha

GH: Tubulin beta

GI: Tubulin alpha

GJ: Tubulin beta

GK: Cilia- and flagella-associated protein 299

GL: Dynein 18 kDa light chain, flagellar outer arm

GM: Dynein 11 kDa light chain, flagellar outer arm

GN: Dynein light chain roadblock LC7a

GO: Cilia- and flagella-associated protein 20

GP: PACRG

GQ: Cilia- and flagella-associated protein 20

GR: PACRG

GS: Cilia- and flagella-associated protein 20

GT: PACRG

GU: Cilia- and flagella-associated protein 20

GV: PACRG

GW: Cilia- and flagella-associated protein 20

GX: PACRG

GY: Dynein light chain roadblock LC7b

GZ: Dynein 8 kDa light chain, flagellar outer arm

Ga: Coiled-coil protein associated with protofilament ribbons

Gb: Cilia- and flagella-associated protein 53

Gc: FAP210

Gd: Meiosis-specific nuclear structural protein 1

Ge: Dynein 8 kDa light chain, flagellar outer arm

Gf: Coiled-coil protein associated with protofilament ribbons

Gg: Dynein axonemal light chain 1

Gh: Actin

Gi: Coiled-coil protein associated with protofilament ribbons

Gj: Coiled-coil protein associated with protofilament ribbons

Gk: Outer dynein arm protein 1

Gl: Heavy chain alpha

Gm: FAP210

Gn: FAP210

Go: Cilia- and flagella-associated protein 45

Gp: Cilia- and flagella-associated protein 45

Gq: Cilia- and flagella-associated protein 45

Gr: FAP34

Gs: Flagellar associated protein

Gt: Flagellar outer dynein arm heavy chain beta

Gu: Dynein 16 kDa light chain, flagellar outer arm

Gv: Protein Flattop homolog

Gw: Protein Flattop homolog

Gx: Protein Flattop homolog

Gy: Nucleoside diphosphate kinase

Gz: Nucleoside diphosphate kinase

H0: Flagellar radial spoke protein 5

H1: Tubulin alpha

H2: Tubulin beta

H3: Tubulin alpha

H4: Tubulin beta

H5: Tubulin alpha

H6: Tubulin beta

H7: Tubulin alpha

H8: Tubulin beta

H9: Tubulin alpha

HA: Flagellar associated protein

HB: Dynein 14 kDa light chain, flagellar outer arm

HD: Flagellar associated protein

HE: Flagellar associated protein

HF: Dynein regulatory complex protein 8

HG: FAP52

HH: Flagellar associated protein

HI: Flagellar associated protein

HJ: Flagellar associated protein

HK: Flagellar associated protein

HL: Flagellar associated protein

HM: Dynein regulatory complex protein 8

HN: FAP143

HO: Flagellar associated protein

HP: FAP166

HQ: FAP166

HS: Flagellar associated protein

HT: Flagellar associated protein

HU: Flagellar associated protein

HV: Flagellar associated protein

HW: Flagellar associated protein

HX: Flagellar radial spoke protein 1

HY: FAP339

HZ: FAP339

Ha: FAP339

Hb: Protofilament ribbon protein of flagellar microtubules

Hc: Protofilament ribbon protein of flagellar microtubules

Hd: Protofilament ribbon protein of flagellar microtubules

He: Protofilament ribbon protein of flagellar microtubules

Hf: Protofilament ribbon protein of flagellar microtubules

Hg: Protofilament ribbon protein of flagellar microtubules

Hh: Flagellar radial spoke protein 1

Hi: Flagellar radial spoke protein 2

Hj: Flagellar associated protein

Hk: Flagellar associated protein

Hl: Flagellar associated protein

Hm: Flagellar radial spoke protein 2

Hn: Flagellar associated protein

Ho: Radial spoke protein 3

Hp: FAP129

Hq: FAP21

Hr: FAP21

Hs: Radial spoke protein 3

Ht: Flagellar associated protein

Hu: Tubulin alpha

Hv: Flagellar associated protein

Hw: FAP306

Hx: Radial spoke protein 4

Hy: Radial spoke protein 4

Hz: Flagellar radial spoke protein 5

I0: Tubulin beta

I1: Tubulin alpha

I2: Tubulin beta

I3: Tubulin alpha

I4: Tubulin beta

I5: Tubulin alpha

I6: Dynein 8 kDa light chain, flagellar outer arm

I7: Tubulin alpha

I8: Tubulin beta

I9: Tubulin alpha

IA: Flagellar radial spoke protein 6

IB: Flagellar radial spoke protein 6

IC: Radial spoke protein 7

ID: Flagellar associated protein

IE: Radial spoke protein 7

IF: Radial spoke protein 9

IG: Flagellar associated protein

IH: Flagellar associated protein

II: Radial spoke protein 9

IJ: Radial spoke protein 9

IK: Tubulin beta

IL: Tubulin alpha

IM: Tubulin beta

IN: Radial spoke protein 9

IO: Radial spoke protein 10

IP: Radial spoke protein 10

IQ: Radial spoke protein 11

IR: Radial spoke protein 11

IS: Peptidyl-prolyl cis-trans isomerase

IT: Radial spoke protein 14

IU: Radial spoke protein 16

IV: Tubulin alpha

IW: Tubulin beta

IX: Tubulin alpha

IY: Tubulin beta

IZ: Tubulin alpha

Ia: Radial spoke protein 16

Ib: UNKNOWN

Ic: Nucleoside diphosphate kinase 6

Id: Nucleoside diphosphate kinase 6

Ie: Cytochrome b5 heme-binding domain-containing protein

If: FAP385

Ig: FAP385

Ih: Radial spoke protein 16

Ii: Tubulin alpha

Ij: Tubulin beta

Ik: Tubulin alpha

Il: Tubulin beta

Im: Tubulin alpha

In: Radial spoke protein 16

Io: Dynein 8 kDa light chain, flagellar outer arm

Ip: Dynein 8 kDa light chain, flagellar outer arm

Iq: Dynein 8 kDa light chain, flagellar outer arm

Ir: Dynein 8 kDa light chain, flagellar outer arm

Is: Dynein 8 kDa light chain, flagellar outer arm

It: Dynein 8 kDa light chain, flagellar outer arm

Iu: Dynein 8 kDa light chain, flagellar outer arm

Iv: Tubulin alpha

Iw: Tubulin beta

Ix: Tubulin alpha

Iy: Tubulin beta

Iz: Tubulin alpha

J0: Tubulin beta

J1: Tubulin alpha

J2: Tubulin beta

J3: Tubulin alpha

J4: Tubulin beta

J5: Tubulin alpha

J6: Tubulin beta

J7: Tubulin alpha

J8: Tubulin beta

J9: Tubulin alpha

JA: FAP207

JB: FAP253

JC: Calmodulin

JD: Actin

JE: 28 kDa inner dynein arm light chain, axonemal

JF: 28 kDa inner dynein arm light chain, axonemal

JG: AAA+ ATPase domain-containing protein

JH: Flagellar radial spoke protein 1

JI: Tubulin alpha

JJ: Tubulin beta

JK: Tubulin alpha

JL: Tubulin beta

JM: Flagellar radial spoke protein 1

JN: Flagellar radial spoke protein 2

JO: Flagellar radial spoke protein 2

JP: Radial spoke protein 3

JQ: Radial spoke protein 3

JR: Radial spoke protein 4

JS: Radial spoke protein 4

JT: Flagellar radial spoke protein 5

JU: Tubulin beta

JV: Tubulin alpha

JW: Tubulin beta

JX: Tubulin alpha

JY: Tubulin beta

JZ: Flagellar radial spoke protein 5

Ja: Flagellar radial spoke protein 6

Jb: Flagellar radial spoke protein 6

Jc: Radial spoke protein 9

Jd: Radial spoke protein 9

Je: Radial spoke protein 9

Jf: Radial spoke protein 9

Jg: Radial spoke protein 10

Jh: Tubulin beta

Ji: Tubulin alpha

Jj: Tubulin beta

Jk: Tubulin alpha

Jl: Tubulin beta

Jm: Radial spoke protein 10

Jn: Peptidyl-prolyl cis-trans isomerase

Jo: Radial spoke protein 16

Jp: Radial spoke protein 16

Jq: Nucleoside diphosphate kinase 6

Jr: Nucleoside diphosphate kinase 6

Js: Cytochrome b5 heme-binding domain-containing protein

Jt: FAP385

Ju: Tubulin beta

Jv: Tubulin alpha

Jw: Tubulin beta

Jx: Tubulin alpha

Jy: Tubulin beta

Jz: FAP385

K0: Tubulin beta

K1: Tubulin alpha

K2: Dynein 18 kDa light chain, flagellar outer arm

K3: Tubulin alpha

K4: Tubulin beta

K5: Tubulin alpha

K6: Tubulin beta

K7: Tubulin alpha

K8: Tubulin beta

K9: Tubulin alpha

KA: Radial spoke protein 16

KB: Radial spoke protein 16

KC: UNKNOWN

KD: Radial spoke protein 7

KE: Radial spoke protein 7

KF: Radial spoke protein 11

KG: Radial spoke protein 11

KH: Tubulin beta

KI: Tubulin alpha

KJ: Tubulin beta

KK: Tubulin alpha

KL: Tubulin beta

KM: Dynein 8 kDa light chain, flagellar outer arm

KN: Dynein 8 kDa light chain, flagellar outer arm

KO: Dynein 8 kDa light chain, flagellar outer arm

KP: Dynein 8 kDa light chain, flagellar outer arm

KQ: Dynein 8 kDa light chain, flagellar outer arm

KR: Dynein 8 kDa light chain, flagellar outer arm

KS: Dynein 8 kDa light chain, flagellar outer arm

KT: Dynein 8 kDa light chain, flagellar outer arm

KU: Tubulin beta

KV: Tubulin alpha

KW: Tubulin beta

KX: Tubulin alpha

KY: Tubulin beta

KZ: Radial spoke protein 15

Ka: FAP207

Kb: Radial spike protein 8

Kc: Dynein 8 kDa light chain, flagellar outer arm

Kd: Dynein 8 kDa light chain, flagellar outer arm

Ke: Dynein 8 kDa light chain, flagellar outer arm

Kf: Dynein 8 kDa light chain, flagellar outer arm

Kg: DHC_N2 domain-containing protein

Kh: Tubulin beta

Ki: Tubulin alpha

Kj: Tubulin beta

Kk: Tubulin alpha

Kl: Tubulin beta

Km: Actin

Kn: 28 kDa inner dynein arm light chain, axonemal

Ko: 28 kDa inner dynein arm light chain, axonemal

Kp: Flagellar-associated protein 59

Kq: Flagellar-associated protein 172

Kr: Flagellar outer dynein arm heavy chain beta

Ks: Dynein gamma chain, flagellar outer arm

Kt: Dynein, 78 kDa intermediate chain, flagellar outer arm

Ku: WD_REPEATS_REGION domain-containing protein

Kv: Tubulin beta

Kw: Tubulin alpha

Kx: Tubulin beta

Ky: Tubulin alpha

Kz: Flagellar outer dynein arm light chain 2

L0: Tubulin beta

L1: Tubulin alpha

L2: Tubulin beta

L3: Tubulin alpha

L4: Tubulin beta

L5: Tubulin alpha

L6: Tubulin beta

L7: Tubulin alpha

L8: Dynein light chain roadblock LC7a

L9: Tubulin alpha

LA: Dynein 11 kDa light chain, flagellar outer arm

LB: Dynein light chain roadblock LC7a

LC: Dynein light chain roadblock LC7b

LD: Dynein 8 kDa light chain, flagellar outer arm

LE: Dynein 8 kDa light chain, flagellar outer arm

LF: Dynein 8 kDa light chain, flagellar outer arm

LG: Dynein 8 kDa light chain, flagellar outer arm

LH: Tubulin beta

LI: Tubulin alpha

LJ: Tubulin beta

LK: Tubulin alpha

LL: Dynein light chain 9

LM: Dynein light chain 10

LN: Outer dynein arm-docking complex protein DC3

LO: Flagellar associated protein

LP: Cilia- and flagella-associated protein 58

LQ: Cilia- and flagella-associated protein 58

LR: Flagellar-associated protein 59

LS: Flagellar-associated protein 172

LT: Tubulin beta

LU: Tubulin alpha

LV: Tubulin beta

LW: Tubulin alpha

LX: Tubulin beta

LY: FAP251

LZ: Cilia- and flagella-associated protein 61

La: 28 kDa inner dynein arm light chain, axonemal

Lb: 28 kDa inner dynein arm light chain, axonemal

Lc: DHC2

Ld: Actin

Le: Subunit of axonemal inner dynein

Lf: Subunit of axonemal inner dynein

Lg: CFAP91 domain-containing protein

Lh: Tubulin beta

Li: Tubulin alpha

Lj: Tubulin beta

Lk: Tubulin alpha

Ll: Dynein axonemal light chain 1

Lm: Heavy chain alpha

Ln: Flagellar outer dynein arm heavy chain beta

Lo: Dynein gamma chain, flagellar outer arm

Lp: Dynein, 78 kDa intermediate chain, flagellar outer arm

Lq: WD_REPEATS_REGION domain-containing protein

Lr: Flagellar outer dynein arm light chain 2

Ls: Dynein 16 kDa light chain, flagellar outer arm

Lt: Dynein 18 kDa light chain, flagellar outer arm

Lu: Tubulin beta

Lv: Tubulin alpha

Lw: Tubulin beta

Lx: Tubulin alpha

Ly: Dynein 14 kDa light chain, flagellar outer arm

Lz: Dynein 11 kDa light chain, flagellar outer arm

M0: Tubulin beta

M1: Tubulin alpha

M2: Tubulin beta

M3: Tubulin alpha

M4: Tubulin beta

M5: Tubulin alpha

M6: Tubulin beta

M7: Tubulin alpha

M8: Tubulin beta

M9: Tubulin alpha

MA: Dynein light chain roadblock LC7b

MB: Dynein 8 kDa light chain, flagellar outer arm

MC: Dynein 8 kDa light chain, flagellar outer arm

MD: Dynein 8 kDa light chain, flagellar outer arm

ME: Dynein 8 kDa light chain, flagellar outer arm

MF: Tubulin alpha

MG: Tubulin beta

MH: Tubulin alpha

MI: Tubulin beta

MJ: Tubulin alpha

MK: Dynein light chain 9

ML: Dynein light chain 10

MM: Outer dynein arm-docking complex subunit 1

MN: Outer dynein arm protein 1

MO: Outer dynein arm-docking complex protein DC3

MP: FAP44

MQ: FAP43

MR: Outer dynein arm-docking complex subunit 1

MS: Tubulin alpha

MT: Tubulin beta

MU: Tubulin alpha

MV: Tubulin beta

MW: Tubulin alpha

MX: Outer dynein arm protein 1

MY: Outer dynein arm-docking complex subunit 1

MZ: Outer dynein arm protein 1

Ma: Outer dynein arm-docking complex subunit 1

Mb: Outer dynein arm protein 1

Mc: Actin

Md: Caltractin

Me: AAA+ ATPase domain-containing protein

Mf: Tubulin alpha

Mg: Tubulin beta

Mh: Tubulin alpha

Mi: Tubulin beta

Mj: Tubulin alpha

Mk: Actin

Ml: Caltractin

Mm: AAA+ ATPase domain-containing protein

Ms: Tubulin alpha

Mt: Tubulin beta

Mu: Tubulin alpha

Mv: Tubulin beta

Mw: Tubulin alpha

N0: Tubulin beta

N1: Tubulin alpha

N2: Tubulin beta

N3: Tubulin alpha

N5: Tubulin alpha

N6: Tubulin beta

N7: Tubulin alpha

N8: Tubulin beta

N9: Tubulin alpha

NF: Tubulin beta

NG: Tubulin alpha

NH: Tubulin beta

NI: Tubulin alpha

NJ: Tubulin beta

NS: Tubulin beta

NT: Tubulin alpha

NU: Tubulin beta

NV: Tubulin alpha

NW: Tubulin beta

Nf: Tubulin beta

Ng: Tubulin alpha

Nh: Tubulin beta

Ni: Tubulin alpha

Nj: Tubulin beta

Ns: Cilia- and flagella-associated protein 20

Nt: PACRG

Nu: Cilia- and flagella-associated protein 20

Nv: PACRG

Nw: Cilia- and flagella-associated protein 20

Ny: Cilia- and flagella-associated protein 53

Nz: Flagellar associated protein

O0: Tubulin beta

O1: Tubulin alpha

O2: Tubulin beta

O3: Tubulin alpha

O4: Tubulin beta

O5: Tubulin alpha

O6: Tubulin beta

O7: Tubulin alpha

O8: Tubulin beta

O9: Tubulin alpha

OA: Meiosis-specific nuclear structural protein 1

OD: Cilia- and flagella-associated protein 45

OG: Coiled-coil protein associated with protofilament ribbons

OL: Cilia- and flagella-associated protein 53

ON: Cilia- and flagella-associated protein 53

OO: Cilia- and flagella-associated protein 45

OQ: FAP34

OV: Protein Flattop homolog

OY: Nucleoside diphosphate kinase

OZ: Flagellar associated protein

Oc: Flagellar associated protein

Od: Flagellar associated protein

Oi: Flagellar associated protein

Oj: Flagellar associated protein

Ok: Flagellar associated protein

Om: FAP143

On: Flagellar associated protein

Op: FAP166

Ot: Flagellar associated protein

Ou: Flagellar associated protein

Ov: Flagellar associated protein

Oy: FAP339

Oz: FAP339

P3: Tubulin alpha

P4: Tubulin beta

P5: Tubulin alpha

P6: Tubulin beta

P7: Tubulin alpha

P8: Tubulin beta

P9: Tubulin alpha

PD: Protofilament ribbon protein of flagellar microtubules

PE: Protofilament ribbon protein of flagellar microtubules

PF: Protofilament ribbon protein of flagellar microtubules

PK: Flagellar associated protein

PQ: FAP21

PV: Flagellar associated protein

PW: FAP306

Pe: Flagellar associated protein

Pi: Flagellar associated protein

Q0: Tubulin beta

Q1: Tubulin alpha

Q2: Tubulin beta

Q3: Tubulin alpha

Q4: Tubulin beta

Q5: Tubulin alpha

Q6: Tubulin beta

Q8: Tubulin beta

Q9: Tubulin alpha

R0: Tubulin beta

R1: Tubulin alpha

R2: Tubulin beta

R3: Tubulin alpha

R4: Tubulin beta

R5: Tubulin alpha

R6: Tubulin beta

R7: Tubulin alpha

R8: Tubulin beta

R9: Tubulin alpha

S0: Tubulin beta

S1: Tubulin alpha

S2: Tubulin beta

S4: Tubulin beta

S5: Tubulin alpha

S6: Tubulin beta

S7: Tubulin alpha

S8: Tubulin beta

S9: Tubulin alpha

T0: Tubulin beta

T1: Tubulin alpha

T2: Tubulin beta

T3: Tubulin alpha

T4: Tubulin beta

T5: Tubulin alpha

T6: Tubulin beta

T7: Tubulin alpha

T8: Tubulin beta

U1: Tubulin alpha

U2: Tubulin beta

U3: Tubulin alpha

U4: Tubulin beta

U5: Tubulin alpha

U6: Tubulin beta

U7: Tubulin alpha

U8: Tubulin beta

U9: Tubulin alpha

V0: Tubulin beta

V1: Tubulin alpha

V2: Tubulin beta

V3: Tubulin alpha

V4: Tubulin beta

V6: Tubulin beta

V7: Tubulin alpha

V8: Tubulin beta

V9: Tubulin alpha

W0: Tubulin beta

W1: Tubulin alpha

W2: Tubulin beta

W3: Tubulin alpha

W4: Tubulin beta

W5: Tubulin alpha

W6: Tubulin beta

W7: Tubulin alpha

W8: Tubulin beta

W9: Tubulin alpha

X0: Tubulin beta

X2: Tubulin beta

X3: Tubulin alpha

X4: Tubulin beta

X5: Tubulin alpha

X6: Tubulin beta

X7: Tubulin alpha

X8: Tubulin beta

X9: Tubulin alpha

Y0: Tubulin beta

Y1: Tubulin alpha

Y2: Tubulin beta

Y3: Tubulin alpha

Y4: Tubulin beta

Y5: Tubulin alpha

Y6: Tubulin beta

Y8: Tubulin beta

Y9: Tubulin alpha

Z0: Tubulin beta

Z1: Tubulin alpha

Z2: Tubulin beta

Z3: Tubulin alpha

Z4: Tubulin beta

Z5: Tubulin alpha

Z6: Tubulin beta

Z7: Tubulin alpha

Z8: Tubulin beta

Z9: Tubulin alpha

za: Cilia- and flagella-associated protein 77

zb: Cilia- and flagella-associated protein 77

zc: Cilia- and flagella-associated protein 77

zd: Cilia- and flagella-associated protein 77

ze: Cilia- and flagella-associated protein 77

zf: Cilia- and flagella-associated protein 77

zg: Cilia- and flagella-associated protein 77

zh: Cilia- and flagella-associated protein 77

zi: Cilia- and flagella-associated protein 77

zj: Flagellar associated protein

zk: Flagellar associated protein

zl: Flagellar associated protein

zn: FAP306

zo: FAP306

zp: Flagellar associated protein

zq: Flagellar associated protein

zr: Flagellar associated protein

zs: Flagellar associated protein

zt: FAP1

zu: FAP1

zv: FAP1

zw: FAP1

zx: FAP1

zy: FAP1

zz: FAP1

hetero molecules

Summary:

• 63.5 MDa, 1177 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	63,486,349	2186
Polymers	63,154,273	1177
Non-polymers	332,076	1009
Water	0

1

Summary:

• Idetical with deposited unit
• defined by author

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555		1

Components

Protein , 51 types, 909 molecules 0A 0C 0E 0G 0I 0K 0M 0O 0Q 0S 0U 0W 0Y 1A 1C 1E 1G 1K 1M 1O 1Q 1S 1U 1W 2A 2C 2E 2G 2I 2K ...

#1: Protein

0A 0C 0E 0G 0I 0K 0M 0O 0Q 0S 0U 0W 0Y 1A 1C 1E 1G 1K 1M ...
Tubulin beta / Tubulin beta-1/beta-2 chain / Beta-tubulin

Details:

Mass: 49665.809 Da / Num. of mol.: 340 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P04690

#2: Protein

0B 0F 0H 0J 0L 0N 0P 0R 0V 0X 0Z 1B 1D 1F 1H 1L 1N 1P 1R ...
Tubulin alpha / Tubulin alpha-1 chain

Details:

Mass: 49638.008 Da / Num. of mol.: 337 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P09204

#3: Protein

3V 3X 3Z 4B 4D 4F 4H 4J GP GR GT GV GX Nt Nv
PACRG / Parkin-co-regulated gene product

Details:

Mass: 34215.148 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: B1B601

#5: Protein

4O 5D Gd OA
Meiosis-specific nuclear structural protein 1

Details:

Mass: 59870.238 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CRG7

#7: Protein

4R Ga Gf Gi Gj OG
Coiled-coil protein associated with protofilament ribbons / Coiled coil / Flagellar protofilament ribbon protein

Details:

Mass: 42712.711 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q9M6B0

#9: Protein

5E Gr OQ FAP34

Details:

Mass: 29859.654 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E5X9

#11: Protein

5H 5I 5J 5K Gv Gw Gx OV
Protein Flattop homolog / Cilia- and flagella-associated protein 126 / Flagellum-associated protein 126

Details:

Mass: 15435.397 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IVJ1

#12: Protein

5L 5M 5N Ad Ae Af Eo HG
FAP52

Details:

Mass: 68546.508 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D260

#17: Protein

6E HN Om FAP143

Details:

Mass: 29216.520 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E1X6

#19: Protein

6G 6H HP HQ Op
FAP166

Details:

Mass: 23303.205 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DZF0

#21: Protein

6Q 6R 6S HY HZ Ha Oy Oz
FAP339

Details:

Mass: 10073.385 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DTN6

#22: Protein

6T 6U 6V 6W 6X 6Y 6Z Hb Hc Hd He Hf Hg PD PE PF
Protofilament ribbon protein of flagellar microtubules / RIB72 protein / p72

Details:

Mass: 72082.609 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q8LKK4

#27: Protein

7I 7J Hp FAP129

Details:

Mass: 51330.992 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DZI2

#28: Protein

7K Hq Hr PQ
FAP21

Details:

Mass: 49657.867 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CNK8

#31: Protein

7Q Hw PW zn zo
FAP306

Details:

Mass: 20949.049 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D7C7

#34: Protein

AA Cb Gg Ll
Dynein axonemal light chain 1 / DNAL1 / Flagellar outer arm dynein light chain 1 / ODA-LC protein LC1

Details:

Mass: 22179.570 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q9XHH2

#37: Protein

AD AX Fa Ks Lo
Dynein gamma chain, flagellar outer arm

Details:

Mass: 513491.406 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39575

#38: Protein

AE IC97/Casc1 N-terminal domain-containing protein

Details:

Mass: 81724.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CYR5

#40: Protein

AG IC140

Details:

Mass: 109942.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J3B6

#44: Protein

AL AM AN AO AP AQ Be Bp Bq Br CB CC CD CO FA FB GZ Ge I6 ...
Dynein 8 kDa light chain, flagellar outer arm

Details:

Mass: 10336.775 Da / Num. of mol.: 46 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39580

#46: Protein

AS Inner arm dynein light chain

Details:

Mass: 13709.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q6IET4

#50: Protein

Aa Ab Fo Kt Lp
Dynein, 78 kDa intermediate chain, flagellar outer arm / / IC78

Details:

Mass: 76654.711 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39578

#53: Protein

BC BD GL K2 Lt
Dynein 18 kDa light chain, flagellar outer arm

Details:

Mass: 17807.119 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39584

#54: Protein

BE BP GM LA Lz
Dynein 11 kDa light chain, flagellar outer arm

Details:

Mass: 13876.627 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39579

#56: Protein

Cc Cd Gk MN MX MZ Mb
Outer dynein arm protein 1 / Docking complex component 2

Details:

Mass: 62292.305 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JF70

#58: Protein

Co Dynein-1-beta heavy chain, flagellar inner arm I1 complex / 1-beta DHC / Dynein-1 / subspecies f

Details:

Mass: 511297.469 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q9MBF8

#60: Protein

D9 Inner-arm dynein f/I1 light chain

Details:

Mass: 26239.871 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E783

#61: Protein

DA DP Gl Lm
Heavy chain alpha

Details:

Mass: 504949.312 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DV97

#63: Protein

DC Dc HB Ly
Dynein 14 kDa light chain, flagellar outer arm

Details:

Mass: 14195.328 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39591

#64: Protein

DO Dn Gu Ls
Dynein 16 kDa light chain, flagellar outer arm

Details:

Mass: 17391.002 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39592

#65: Protein

Do MP FAP44

Details:

Mass: 222191.750 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DCF8

#66: Protein

Dp MQ FAP43

Details:

Mass: 199676.750 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CWA0

#72: Protein

Ea ATPase AAA-type core domain-containing protein

Details:

Mass: 94896.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DJI6

#77: Protein

Ez DRC5

Details:

Mass: 43558.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E7N9

#79: Protein

FO JC Calmodulin /

Details:

Mass: 18317.215 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IDP6

#80: Protein

FZ Md Ml Caltractin / Centrin / 20 kDa calcium-binding protein / Centrin

Details:

Mass: 19488.980 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P05434

#85: Protein

Gc Gm Gn FAP210

Details:

Mass: 53637.711 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E0E3

#86: Protein

Gh JD Km Ld Mc Mk
Actin /

Details:

Mass: 41881.984 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P53498

#97: Protein

IS Jn Peptidyl-prolyl cis-trans isomerase / Prolyl isomerase / PPIase

Details:

Mass: 19632.256 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I2U9, peptidylprolyl isomerase

#102: Protein

Ie Js Cytochrome b5 heme-binding domain-containing protein

Details:

Mass: 26609.070 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DCN8

#103: Protein

If Ig Jt Jz
FAP385

Details:

Mass: 8111.163 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JDM3

#104: Protein

JA Ka FAP207

Details:

Mass: 28418.410 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DJP7

#105: Protein

JB FAP253

Details:

Mass: 75037.945 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D359

#106: Protein

JE JF Kn Ko La Lb
28 kDa inner dynein arm light chain, axonemal / p28

Details:

Mass: 28691.717 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q39604

#108: Protein

KC UNKNOWN

Details:

Mass: 6145.567 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant)

#110: Protein

Kb Radial spike protein 8 / Radial spoke protein 8

Details:

Mass: 40537.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU6

#111: Protein

Kg DHC_N2 domain-containing protein

Details:

Mass: 465175.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E486

#116: Protein

LY FAP251

Details:

Mass: 97477.547 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DY12

#118: Protein

Lc DHC2

Details:

Mass: 467544.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DE97

#121: Protein

Lg CFAP91 domain-containing protein / FAP91

Details:

Mass: 134440.578 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DJU2

#127: Protein

zt zu zv zw zx zy zz
FAP1

Details:

Mass: 21706.502 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J0T8

Cilia- and flagella-associated protein ... , 9 types, 41 molecules 3W 3Y 4A 4C 4E 4G 4I GO GQ GS GU GW Ns Nu Nw 4X 5C Go Gp Gq OD OO AV AW GK Gb Ny OL ON LP ...

#4: Protein

3W 3Y 4A 4C 4E 4G 4I GO GQ GS GU GW Ns Nu Nw
Cilia- and flagella-associated protein 20 / Basal body up-regulated protein 22 / Bug22p / Flagellar-associated protein 20

Details:

Mass: 22193.566 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IU92

#8: Protein

4X 5C Go Gp Gq OD OO
Cilia- and flagella-associated protein 45 / Flagellum-associated protein 45

Details:

Mass: 59041.469 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I9E8

#48: Protein

AV Cilia- and flagella-associated protein 73 / Flagella-associated protein 73 / Modifier of inner arms 2 protein / Mia2p

Details:

Mass: 35671.414 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: M1V4Y8

#49: Protein

AW Cilia- and flagella-associated protein 100 / Flagella-associated protein 100 / Modifier of inner arms 1 protein / Mia1p

Details:

Mass: 65268.512 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I4E9

#83: Protein

GK Cilia- and flagella-associated protein 299

Details:

Mass: 27467.602 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D8T3

#84: Protein

Gb Ny OL ON
Cilia- and flagella-associated protein 53

Details:

Mass: 56655.496 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IRJ7

#115: Protein

LP LQ Cilia- and flagella-associated protein 58

Details:

Mass: 102152.039 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8HUA7

#117: Protein

LZ Cilia- and flagella-associated protein 61 / Flagellar-associated protein 61

Details:

Mass: 121435.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IF44

#124: Protein

za zb zc zd ze zf zg zh zi
Cilia- and flagella-associated protein 77 / Flagellar-associated protein 77

Details:

Mass: 29213.932 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IB22

Flagellar associated ... , 19 types, 85 molecules 4Q 4W Nz 5F 7B Gs 5Q HA OZ 5S 5T 5U 5V HD HE Oc Od 5X 5Y 5Z 6A 6B 6C 6D HH HI HJ HK HL Oi ...

#6: Protein

4Q 4W Nz Flagellar associated protein

Details:

Mass: 53126.352 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J0X0

#10: Protein

5F 7B Gs Flagellar associated protein

Details:

Mass: 43741.223 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IY81

#14: Protein

5Q HA OZ Flagellar associated protein

Details:

Mass: 22228.705 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J250

#15: Protein

5S 5T 5U 5V HD HE Oc Od
Flagellar associated protein

Details:

Mass: 27019.803 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J098

#16: Protein

5X 5Y 5Z 6A 6B 6C 6D HH HI HJ HK HL Oi Oj Ok
Flagellar associated protein

Details:

Mass: 26647.648 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JDK1

#18: Protein

6F HO On zp zq
Flagellar associated protein

Details:

Mass: 42849.578 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8HRH3

#20: Protein

6I 6J 6K 6L 6M 6N 6O 6P HS HT HU HV HW Ot Ou Ov
Flagellar associated protein

Details:

Mass: 39507.379 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I1S7

#23: Protein

7C Hj Flagellar associated protein

Details:

Mass: 12740.544 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A835TDA6

#24: Protein

7D 7E Hk Hl PK
Flagellar associated protein

Details:

Mass: 15920.962 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JF23

#25: Protein

7G Hn Flagellar associated protein

Details:

Mass: 25576.527 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8HSW0

#26: Protein

7H 7X Flagellar associated protein

Details:

Mass: 48843.969 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JHR6

#29: Protein

7M 7N Ht Flagellar associated protein

Details:

Mass: 15469.539 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IXN7

#30: Protein

7P Hv PV Flagellar associated protein

Details:

Mass: 25937.781 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JC52

#32: Protein

7Y ID Pe Flagellar associated protein

Details:

Mass: 35568.691 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8HPK6

#33: Protein

8B 8C IG IH Pi
Flagellar associated protein

Details:

Mass: 27760.064 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8ICC1

#73: Protein

Eb Flagellar associated protein

Details:

Mass: 177706.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CXC4

#114: Protein

LO Flagellar associated protein

Details:

Mass: 267418.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D574

#125: Protein

zj zk zl Flagellar associated protein

Details:

Mass: 32078.156 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J6L0

#126: Protein

zr zs Flagellar associated protein

Details:

Mass: 16209.316 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JG37

Nucleoside diphosphate ... , 2 types, 9 molecules 5O 5P Gy Gz OY Ic Id Jq Jr

#13: Protein

5O 5P Gy Gz OY
Nucleoside diphosphate kinase / Nucleoside-diphosphate kinase

Details:

Mass: 41994.797 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JGW5, nucleoside-diphosphate kinase

#101: Protein

Ic Id Jq Jr
Nucleoside diphosphate kinase 6 / Nucleoside-diphosphate kinase / radial spoke protein 23

Details:

Mass: 61422.211 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q69B19, nucleoside-diphosphate kinase

Outer dynein arm-docking complex ... , 2 types, 12 molecules AB AY Fb MM MR MY Ma AC AZ Fn LN MO

#35: Protein

AB AY Fb MM MR MY Ma
Outer dynein arm-docking complex subunit 1

Details:

Mass: 83518.180 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant)
References: UniProt: A8IPZ5, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor

#36: Protein

AC AZ Fn LN MO
Outer dynein arm-docking complex protein DC3 / Outer dynein arm-docking complex subunit 3

Details:

Mass: 21371.311 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q7Y0H2

Axonemal inner arm ... , 2 types, 2 molecules AF AH

#39: Protein

AF Axonemal inner arm I1 intermediate chain dynein IC138

Details:

Mass: 111279.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q5XPS1

#41: Protein

AH Axonemal inner arm dynein I1/f subunit

Details:

Mass: 31912.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I2N3

Dynein light chain ... , 5 types, 23 molecules AI Bc Bd GY LC MA AK BQ BR GN L8 LB AR AU Ca FN LM ML CP CQ FM LL MK

#42: Protein

AI Bc Bd GY LC MA
Dynein light chain roadblock LC7b

Details:

Mass: 11132.679 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IY95

#43: Protein

AK BQ BR GN L8 LB
Dynein light chain roadblock LC7a

Details:

Mass: 11946.708 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q9SWQ6

#45: Protein

AR Dynein light chain Tctex1

Details:

Mass: 12680.427 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: O64980

#47: Protein

AU Ca FN LM ML
Dynein light chain 10

Details:

Mass: 12101.117 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J5C4

#55: Protein

CP CQ FM LL MK
Dynein light chain 9

Details:

Mass: 12993.606 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q2VIY5

WD REPEATS REGION domain-containing ... , 2 types, 7 molecules Ac Aq Fz Ku Lq Cp Cq

#51: Protein

Ac Aq Fz Ku Lq
WD_REPEATS_REGION domain-containing protein

Details:

Mass: 63608.086 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IJZ3

#59: Protein

Cp Cq WD_REPEATS_REGION domain-containing protein

Details:

Mass: 146542.375 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DTF4

AAA+ ATPase domain-containing ... , 5 types, 5 molecules Cn Fm JG Me Mm

#57: Protein

Cn AAA+ ATPase domain-containing protein

Details:

Mass: 523363.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D1N8

#81: Protein

Fm AAA+ ATPase domain-containing protein

Details:

Mass: 468877.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CYE9

#107: Protein

JG AAA+ ATPase domain-containing protein

Details:

Mass: 478234.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DSC5

#122: Protein

Me AAA+ ATPase domain-containing protein

Details:

Mass: 464441.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E2P7

#123: Protein

Mm AAA+ ATPase domain-containing protein

Details:

Mass: 470774.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CUR8

Dynein regulatory complex protein ... , 4 types, 6 molecules EA EZ HF HM En Ey

#67: Protein

EA Dynein regulatory complex protein 1

Details:

Mass: 79454.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P0DL09

#71: Protein

EZ HF HM Dynein regulatory complex protein 8 / Flagellar associated protein 200

Details:

Mass: 18671.031 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J3A0

#75: Protein

En Dynein regulatory complex protein 9

Details:

Mass: 46481.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E6B1

#76: Protein

Ey Dynein regulatory complex protein 10 / Flagellar associated protein 84

Details:

Mass: 40992.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J0N6

Dynein regulatory complex subunit ... , 4 types, 5 molecules EB EM EN EO Em

#68: Protein

EB Dynein regulatory complex subunit 2 / Coiled-coil domain-containing protein 65 homolog / Flagellar-associated protein 250

Details:

Mass: 64986.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JB22

#69: Protein

EM Dynein regulatory complex subunit 6 / Flagellar associated protein 169

Details:

Mass: 28524.879 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JHD7

#70: Protein

EN EO Dynein regulatory complex subunit 4 / Growth arrest-specific protein 8 homolog / Protein PF2

Details:

Mass: 55207.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q7XJ96

#74: Protein

Em Dynein regulatory complex subunit 3 / Flagellar-associated protein 134

Details:

Mass: 60478.004 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IVX2

DUF4201 domain-containing ... , 2 types, 2 molecules F4 Fy

#78: Protein

F4 DUF4201 domain-containing protein

Details:

Mass: 45346.332 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CQ22

#82: Protein

Fy DUF4201 domain-containing protein

Details:

Mass: 55159.035 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DWC0

Flagellar radial spoke protein ... , 4 types, 16 molecules H0 Hz JT JZ HX Hh JH JM Hi Hm JN JO IA IB Ja Jb

#87: Protein

H0 Hz JT JZ
Flagellar radial spoke protein 5

Details:

Mass: 55403.551 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU7, Oxidoreductases

#88: Protein

HX Hh JH JM
Flagellar radial spoke protein 1

Details:

Mass: 87873.445 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU0

#89: Protein

Hi Hm JN JO
Flagellar radial spoke protein 2

Details:

Mass: 77445.297 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q6UBQ3

#92: Protein

IA IB Ja Jb
Flagellar radial spoke protein 6

Details:

Mass: 48884.551 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q01657

Radial spoke protein ... , 9 types, 38 molecules Ho Hs JP JQ Hx Hy JR JS IC IE KD KE IF II IJ IN Jc Jd Je Jf IO IP Jg Jm IQ IR KF KG IT IU ...

#90: Protein

Ho Hs JP JQ
Radial spoke protein 3 /

Details:

Mass: 56856.688 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J2J7

#91: Protein

Hx Hy JR JS
Radial spoke protein 4 /

Details:

Mass: 49843.770 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8I550

#93: Protein

IC IE KD KE
Radial spoke protein 7 /

Details:

Mass: 54781.898 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DL29

#94: Protein

IF II IJ IN Jc Jd Je Jf
Radial spoke protein 9 /

Details:

Mass: 29572.348 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU5

#95: Protein

IO IP Jg Jm
Radial spoke protein 10 /

Details:

Mass: 23553.943 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU4

#96: Protein

IQ IR KF KG
Radial spoke protein 11 /

Details:

Mass: 21504.635 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q27YU3

#98: Protein

IT Radial spoke protein 14 / / Flagellar-associated protein 132

Details:

Mass: 40597.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8HNV0

#99: Protein

IU Ia Ih In Jo Jp KA KB
Radial spoke protein 16 /

Details:

Mass: 39052.676 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IKR9

#109: Protein

KZ Radial spoke protein 15 /

Details:

Mass: 35302.625 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DEQ5

Flagellar-associated protein ... , 2 types, 4 molecules Kp LR Kq LS

#112: Protein

Kp LR Flagellar-associated protein 59 / CCDC39

Details:

Mass: 105504.469 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A0A1H1F6

#113: Protein

Kq LS Flagellar-associated protein 172 / CCDC40

Details:

Mass: 101385.719 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A0A1GYE8

Subunit of axonemal inner ... , 2 types, 2 molecules Le Lf

#119: Protein

Le Subunit of axonemal inner dynein

Details:

Mass: 40962.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A4PET3

#120: Protein

Lf Subunit of axonemal inner dynein

Details:

Mass: 46144.758 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A9ZPM1

Flagellar outer dynein arm ... / Antibody / Protein/peptide , 3 types, 11 molecules Ar As GA Kz Lr DB Db Gt Kr Ln Ib

#100: Protein/peptide

Ib UNKNOWN

Details:

Mass: 2911.580 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant)

#52: Protein

Ar As GA Kz Lr
Flagellar outer dynein arm light chain 2 / Mr19 / 000 outer arm dynein light chain

Details:

Mass: 15903.028 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: O04355

#62: Antibody

DB Db Gt Kr Ln
Flagellar outer dynein arm heavy chain beta

Details:

Mass: 520510.906 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J1M5

Non-polymers , 3 types, 1009 molecules

#128: Chemical

0A-501 0C-501 0F-501 0I-502 0K-501 0M-501 0O-501 0Q-502 0R-502 0V-501 0Y-501 1A-501 1C-501 1D-501 1G-501 1H-501 1L-501 1N-501 1Q-501 1S-501 ...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate

Details:

Mass: 523.180 Da / Num. of mol.: 331 / Source method: obtained synthetically / Formula: C₁₀H₁₆N₅O₁₄P₃ / Comment: GTP, energy-carrying molecule*YM

#129: Chemical

0A-502 0C-502 0E-501 0G-501 0I-503 0K-502 0M-502 0O-502 0Q-503 0S-501 0U-501 0W-501 0Y-502 1A-502 1C-502 1E-501 1G-502 1K-501 1M-501 1O-501 ...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE / Guanosine diphosphate

Details:

Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 340 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₁P₂ / Comment: GDP, energy-carrying molecule*YM

#130: Chemical

0B-501 0F-502 0I-501 0J-501 0L-501 0N-501 0Q-501 0R-501 0V-502 0X-501 0Z-501 1B-501 1D-502 1F-501 1H-502 1H-503 1L-502 1N-502 1P-501 1R-501 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 338 / Source method: obtained synthetically / Formula: Mg

Details

• Has ligand of interest: N

Experimental details

Experiment

• Experiment: Method: ELECTRON MICROSCOPY
• EM experiment: Aggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

Sample preparation

• Component: Name: 96-nm repeat unit of doublet microtubules from the flagella of Chlamydomonas reinhardtii; Type: COMPLEX / Entity ID: #1-#127 / Source: NATURAL
• Molecular weight: Experimental value: NO
• Source (natural): Organism: Chlamydomonas reinhardtii (plant)
• Buffer solution: pH: 7.4

Buffer component

ID	Conc.	Name	Formula	Buffer-ID
1	30 mM	HEPES		1
2	25 mM	potassium chloride	KCl	1
3	5 mM	magnesium sulfate	MgSO4	1
4	0.5 mM	EGTA		1

• Specimen: Conc.: 20 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES; Details: Axonemes were splayed using 10 mM ATP and 0.75 mM CaCl2 for one hour at room temperature before centrifugation and resuspension.
• Specimen support: Grid material: COPPER / Grid type: C-flat-1.2/1.3
• Vitrification: Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 298 K; Details: 2.5 microliters of splayed axoneme sample at 16-26 mg/mL were applied to glow-discharged C-Flat 1.2/1.3-4Cu grids at 100% humidity. The samples were then incubated 10 s, blotted for 10 s, and plunged into liquid ethane

Electron microscopy imaging

• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company
• Microscopy: Model: TFS KRIOS
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
• Electron lens: Mode: BRIGHT FIELDBright-field microscopy / Nominal magnification: 64000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 500 nm
• Image recording: Average exposure time: 3.7 sec. / Electron dose: 61 e/Ų / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of real images: 36918

Processing

• Software: Name: UCSF ChimeraX / Version: 1.5/v9 / Classification: model building / URL: https://www.rbvi.ucsf.edu/chimerax/ / Os: macOS / Type: package

EM software

ID	Name	Version	Category
1	RELION	3.1.1	particle selection
2	SerialEM	3.6	image acquisition
4	CTFFIND	4	CTF correction
7	Coot	0.9.4.1	model fitting
8	UCSF ChimeraX	1.3	model fitting
10	RELION	3.1.1	initial Euler assignment
11	RELION	3.1.1	final Euler assignment
14	PHENIX	1.19.2-4158	model refinement

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Particle selection: Num. of particles selected: 8546747 ; Details: Particles were manually picked as start/end coordinates.
• 3D reconstruction: Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 261585 / Details: Map resolution varies from 3.1 to 16.7 A. / Symmetry type: POINT