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Yorodumi- PDB-8el6: Light harvesting phycobiliprotein HaPE555 from the cryptophyte He... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8el6 | ||||||||||||
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Title | Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 with an altered helix hA/hY conformation | ||||||||||||
Components |
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Keywords | PHOTOSYNTHESIS / phycobiliprotein / antenna / light harvesting / cryptophyte / algae / globin / CALM / CaRSP / phycoerythrin / Hemiselmis | ||||||||||||
Function / homology | Function and homology information | ||||||||||||
Biological species | Hemiselmis andersenii (eukaryote) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | ||||||||||||
Authors | Rathbone, H.W. / Michie, K.A. / Laos, A.L. / Curmi, P.M.G. | ||||||||||||
Funding support | Australia, 3items
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Citation | Journal: Commun Biol / Year: 2023 Title: Molecular dissection of the soluble photosynthetic antenna from the cryptophyte alga Hemiselmis andersenii. Authors: Rathbone, H.W. / Laos, A.J. / Michie, K.A. / Iranmanesh, H. / Biazik, J. / Goodchild, S.C. / Thordarson, P. / Green, B.R. / Curmi, P.M.G. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8el6.cif.gz | 347.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8el6.ent.gz | 281 KB | Display | PDB format |
PDBx/mmJSON format | 8el6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/el/8el6 ftp://data.pdbj.org/pub/pdb/validation_reports/el/8el6 | HTTPS FTP |
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-Related structure data
Related structure data | 7sutC 8el3C 8el4C 8el5C 4lmxS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Phycoerythrin alpha- ... , 2 types, 4 molecules AFCE
#1: Protein | Mass: 7019.024 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Hemiselmis andersenii (eukaryote) / References: UniProt: U5TBU5 #3: Protein | Mass: 6628.628 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Hemiselmis andersenii (eukaryote) / References: UniProt: U5TBJ3 |
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-Protein , 1 types, 2 molecules BD
#2: Protein | Mass: 18406.949 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Hemiselmis andersenii (eukaryote) / References: UniProt: U5T8W0 |
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-Non-polymers , 3 types, 266 molecules
#4: Chemical | ChemComp-PEB / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.52 Å3/Da / Density % sol: 18.93 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, sitting drop Details: PEG3350 23.6% (w/v), 150 nL mother + 150 nL protein solution, Cryoprotectant of Paratone-N |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.9537 Å |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Feb 23, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.83→45.03 Å / Num. obs: 33901 / % possible obs: 100 % / Redundancy: 7.3 % / Biso Wilson estimate: 18.02 Å2 / CC1/2: 0.994 / Rmerge(I) obs: 0.21 / Net I/σ(I): 6.9 |
Reflection shell | Resolution: 1.83→1.87 Å / Rmerge(I) obs: 1.539 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 2072 / CC1/2: 0.315 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4LMX Resolution: 1.95→45.03 Å / SU ML: 0.2658 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.967 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.16 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→45.03 Å
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Refine LS restraints |
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LS refinement shell |
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