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- PDB-8cem: Structure of bovine native C3, re-refinement -

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Basic information

Entry
Database: PDB / ID: 8cem
TitleStructure of bovine native C3, re-refinement
Components
  • Complement C3 alpha chain
  • Complement C3 beta chain
KeywordsIMMUNE SYSTEM / innate immune system / complement / proteolytic activation / thioester
Function / homology
Function and homology information


: / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / positive regulation of lipid storage / positive regulation of G protein-coupled receptor signaling pathway / positive regulation of phagocytosis, engulfment ...: / C5L2 anaphylatoxin chemotactic receptor binding / regulation of triglyceride biosynthetic process / positive regulation of activation of membrane attack complex / vertebrate eye-specific patterning / positive regulation of apoptotic cell clearance / complement-mediated synapse pruning / positive regulation of lipid storage / positive regulation of G protein-coupled receptor signaling pathway / positive regulation of phagocytosis, engulfment / positive regulation of type IIa hypersensitivity / positive regulation of glucose transmembrane transport / complement-dependent cytotoxicity / complement activation, alternative pathway / complement activation / neuron remodeling / endopeptidase inhibitor activity / amyloid-beta clearance / positive regulation of vascular endothelial growth factor production / complement activation, classical pathway / fatty acid metabolic process / response to bacterium / positive regulation of receptor-mediated endocytosis / positive regulation of angiogenesis / inflammatory response / positive regulation of protein phosphorylation / cell surface / protein-containing complex / extracellular space
Similarity search - Function
Complement C3-like / Complement C3-like, NTR domain / Alpha-2-macroglobulin, conserved site / Alpha-2-macroglobulin family thiolester region signature. / Complement C3/4/5, macroglobulin domain MG1 / Macroglobulin domain MG1 / Anaphylatoxin, complement system domain / Anaphylatoxin/fibulin / Anaphylatoxin, complement system / Anaphylotoxin-like domain ...Complement C3-like / Complement C3-like, NTR domain / Alpha-2-macroglobulin, conserved site / Alpha-2-macroglobulin family thiolester region signature. / Complement C3/4/5, macroglobulin domain MG1 / Macroglobulin domain MG1 / Anaphylatoxin, complement system domain / Anaphylatoxin/fibulin / Anaphylatoxin, complement system / Anaphylotoxin-like domain / Anaphylatoxin domain profile. / Anaphylatoxin homologous domain / Netrin C-terminal Domain / Netrin module, non-TIMP type / UNC-6/NTR/C345C module / Alpha-macroglobulin, receptor-binding / Alpha-macroglobulin, receptor-binding domain superfamily / Macroglobulin domain MG4 / Macroglobulin domain MG3 / A-macroglobulin receptor binding domain / Macroglobulin domain MG4 / Macroglobulin domain MG3 / A-macroglobulin receptor / Netrin domain / NTR domain profile. / Tissue inhibitor of metalloproteinases-like, OB-fold / Alpha-2-macroglobulin / Macroglobulin domain / Alpha-2-macroglobulin, bait region domain / Alpha-macroglobulin-like, TED domain / Alpha-2-macroglobulin family / MG2 domain / A-macroglobulin TED domain / Alpha-2-macroglobulin bait region domain / Alpha-2-Macroglobulin / Alpha-2-macroglobulin family / Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid / Immunoglobulin-like fold
Similarity search - Domain/homology
Biological speciesBos taurus (cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SIRAS / Resolution: 3 Å
AuthorsAndersen, G.R. / Fredslund, F.
Funding support Denmark, 1items
OrganizationGrant numberCountry
LundbeckfondenR155-2015-2666 Denmark
Citation
Journal: J Mol Biol / Year: 2006
Title: The structure of bovine complement component 3 reveals the basis for thioester function.
Authors: Fredslund, F. / Jenner, L. / Husted, L.B. / Nyborg, J. / Andersen, G.R. / Sottrup-Jensen, L.
#1: Journal: Acta Crystallogr.,Sect.D / Year: 2012
Title: Towards automated crystallographic structure refinement with phenix.refine.
Authors: Afonine, P.V. / Grosse-Kunstleve, R.W. / Echols, N. / Headd, J.J. / Moriarty, N.W. / Mustyakimov, M. / Terwilliger, T.C. / Urzhumtsev, A. / Zwart, P.H. / Adams, P.D.
#2: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionFeb 2, 2023Deposition site: PDBE / Processing site: PDBE
SupersessionApr 5, 2023ID: 2B39
Revision 1.0Apr 5, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: Complement C3 beta chain
A: Complement C3 alpha chain
F: Complement C3 beta chain
B: Complement C3 alpha chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)370,5166
Polymers369,3434
Non-polymers1,1732
Water0
1
E: Complement C3 beta chain
A: Complement C3 alpha chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)185,2583
Polymers184,6712
Non-polymers5871
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10750 Å2
ΔGint-47 kcal/mol
Surface area72360 Å2
MethodPISA
2
F: Complement C3 beta chain
B: Complement C3 alpha chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)185,2583
Polymers184,6712
Non-polymers5871
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10900 Å2
ΔGint-43 kcal/mol
Surface area71380 Å2
MethodPISA
Unit cell
Length a, b, c (Å)254.246, 246.857, 113.346
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x+1/2,y+1/2,-z
#4: -x,-y,z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 24 through 662 or resid 672...
d_2ens_1chain "B"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1PROGLNA2 - 640
d_12ens_1GLNALAA3 - 72
d_13ens_1ASPASNB81 - 992
d_14ens_1NAGNAGE1
d_15ens_1NAGNAGE2
d_16ens_1BMABMAE3
d_21ens_1PROGLNC2 - 640
d_22ens_1GLNALAC3 - 72
d_23ens_1ASPASND81 - 992
d_24ens_1NAGNAGF1
d_25ens_1NAGNAGF2
d_26ens_1BMABMAF3

NCS oper: (Code: givenMatrix: (-0.999679219701, 0.0253186404984, -0.000651262065037), (-0.0253206413044, -0.999674022288, 0.00327326848974), (-0.000568175059973, 0.00328870886284, 0.99999443077) ...NCS oper: (Code: given
Matrix: (-0.999679219701, 0.0253186404984, -0.000651262065037), (-0.0253206413044, -0.999674022288, 0.00327326848974), (-0.000568175059973, 0.00328870886284, 0.99999443077)
Vector: 121.837208217, 64.4995503867, -56.7518035685)

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Components

#1: Protein Complement C3 beta chain


Mass: 71474.281 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Details: this is mature C3 with two chains after furin processing, uniprot ID Q2UVX4
Source: (natural) Bos taurus (cattle) / References: UniProt: Q2UVX4
#2: Protein Complement C3 alpha chain


Mass: 113197.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Details: this is mature C3 with two chains after furin processing, uniprot ID Q2UVX4
Source: (natural) Bos taurus (cattle) / References: UniProt: Q2UVX4
#3: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.88 Å3/Da / Density % sol: 74.78 %
Crystal growTemperature: 298 K / Method: vapor diffusion / pH: 7.7 / Details: NaCitrate, Hepes, pH 7.7

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: BESSY / Beamline: 14.2 / Wavelength: 0.9537 Å
DetectorType: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 15, 2004
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 3→40 Å / Num. obs: 138965 / % possible obs: 97.9 % / Observed criterion σ(I): -3 / Redundancy: 4.8 % / Biso Wilson estimate: 68.35 Å2 / CC1/2: 0.95 / Rmerge(I) obs: 0.104 / Net I/σ(I): 12.8
Reflection shellResolution: 3→3.19 Å / Mean I/σ(I) obs: 2.2 / Num. unique obs: 21736 / CC1/2: 0.5 / % possible all: 92

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
CNS1.2phasing
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: SIRAS / Resolution: 3→37.85 Å / SU ML: 0.5462 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 32.846
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2844 1392 1 %
Rwork0.2592 137547 -
obs0.2595 138939 97.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 108.43 Å2
Refinement stepCycle: LAST / Resolution: 3→37.85 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms25617 0 78 0 25695
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.006226194
X-RAY DIFFRACTIONf_angle_d1.009435471
X-RAY DIFFRACTIONf_chiral_restr0.06394025
X-RAY DIFFRACTIONf_plane_restr0.00884571
X-RAY DIFFRACTIONf_dihedral_angle_d5.50383542
Refine LS restraints NCSType: Torsion NCS / Rms dev position: 1.91618532123 Å
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3-3.110.40761200.40311378X-RAY DIFFRACTION81.52
3.11-3.230.43011480.375813625X-RAY DIFFRACTION97.14
3.23-3.380.35621220.345913927X-RAY DIFFRACTION99.48
3.38-3.560.32271420.312514049X-RAY DIFFRACTION99.99
3.56-3.780.32631420.293614078X-RAY DIFFRACTION100
3.78-4.070.32841310.266314072X-RAY DIFFRACTION99.95
4.07-4.480.28881450.237714112X-RAY DIFFRACTION99.85
4.48-5.130.25321570.217214146X-RAY DIFFRACTION99.79
5.13-6.460.27621390.237214251X-RAY DIFFRACTION99.7
6.46-37.850.19281460.196913909X-RAY DIFFRACTION94.56
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.180394554638-0.2926991672590.3872793894022.30002372740.1279497445431.573784316080.1472833723950.2805337944320.5155170890690.675600772828-0.618317702471-0.679654671719-0.1450747599870.1078794298050.3296301617390.8118923492730.1378279860080.3035137601942.08680099380.3549471112792.15615280736-4.53583.98562.403
24.96560122388-0.5164626692021.195322012694.76234462584-0.6787704567463.731297307080.220772472966-0.3045076361050.686677212970.4653624148230.3936218922060.5677163976170.101422877675-0.944773152469-0.4867179272370.6294429448160.374886424959-0.01053334945441.035690304640.1381274002581.1754565530930.6567.93152.712
31.401847220880.13216493663-0.5151456727467.342123594720.3303130849794.69702630443-0.02436296421531.104110583860.0113292809398-0.042997838562-0.1588610610361.678728908380.05154204298090.6073573628280.1529932023761.174700028520.109488434791-0.2207117300261.661334681170.2153551603781.6172617077733.56759.4615.658
41.230221655530.4269082385470.9961803124442.221869911030.836611361667.034721933730.8114050051150.571154248682-0.131626495327-0.0324927987020.38866793428-0.742651315725-0.1951743383411.78232089764-1.136304517940.900244013767-0.0324638556699-0.01027148328281.630565398830.1054402746722.096381155422.68176.91122.667
52.89830479686-1.440262462380.002365175906841.968368942661.227536701723.10659689823-0.3526977173950.03700087947570.605458838120.00775272844675-0.06765025110490.136916270917-0.335288962383-0.2599977455480.4238049389640.6799705757230.274509384029-0.06163719265441.334441997490.3709959721112.24024099807-4.1591.47340.018
63.9873795626-1.00283914383-2.232745545681.81868891290.9473706888525.447264408830.182025166905-0.2743380530871.28563053527-0.2338135275720.1149119542180.471512572352-0.464714082951-0.149979302881-0.2808918799790.8034962991960.29390554606-0.0009842660208160.8742022290060.1635526831081.7837673621438.7683.40741.83
71.28335660174-0.917923394909-1.376888286760.7142207915071.466854632983.03001385769-0.636297640159-0.787931902347-0.412540607014-0.5023754622320.4676909977330.919307270230.874558557482-0.20133418880.161461457090.7640476571090.261872420653-0.0561205277971.282653046620.4977807648042.457276668864.75775.76244.643
86.236069773030.5289530041770.1670679887843.187721042370.3208635335595.375523231970.1015683174520.9510063845240.151099593372-1.28183938429-0.09040859977171.02384669384-0.246811167438-0.804388304255-0.02191349733330.7705532631070.0464692938784-0.2034525184030.6642729906680.002814339654040.52684891088144.02335.97719.735
96.58009569228-0.7648223212770.09248860920566.53185266826-1.056338376143.962428284550.3011414772520.3865319963291.556796125390.163723397246-0.3431526302540.0504903796281-2.019396203370.3562982139020.1516037183071.31249454397-0.02074014212450.1212373969550.5690154456480.07711266522770.79564568765968.93275.44932.03
107.28631434522-0.342972033513-0.4540051915115.2850911428-1.387137946583.04333851129-0.00436285589057-0.08393606268670.4589987323850.0776080546952-0.0432783990978-0.112388638842-0.6953982691380.38062693671-0.03376404780880.540732834803-0.0312614286953-0.08042341119510.321963811639-0.03092757458260.4465211799273.8849.70670.25
113.656727951912.02355986182-0.1376697126264.64244662125-0.7761194001542.847056486340.0341581335199-0.02919963622240.2040765746560.108136436304-0.05452030185120.00530902357344-0.0940442069588-0.2732643741990.05117961925440.5819314507630.156887810993-0.002840717278310.389693964435-0.0388674358470.22457390286661.90344.35736.335
122.823281544910.3348146421310.8962929623984.00544974009-1.088930473223.239700592070.066450085741-0.102289252520.1401272791020.1840009129590.0250198152880.4620319091860.00920113152352-0.720512350725-0.07517641242670.4777237948050.06433883868230.09591999823160.581001876172-0.02064203482860.29576707964146.75138.24363.703
135.882724603860.4669233953380.9891676723925.589667259730.8694076418223.04435957398-0.266748672908-0.904159486796-0.2600905178050.329009448890.251094770215-1.38928541073-0.2536955737881.407507391570.02697997796410.798124072224-0.1277333723670.004838850843921.744097652750.09484120905721.07392998337100.30356.3641.403
143.218648360273.08209915373-0.5398914204182.93944542937-0.8405095573143.281728352460.691056670275-0.33038171223-0.5706648005030.159838697951-0.852453957854-0.1206603024150.114833760681-0.02430749181650.2077986024611.373115346410.289594790324-0.3496829441561.16769836686-0.1512590254451.34489142583127.989-16.7885.802
157.29476246485-1.64679197457-0.5224872910245.351538497990.7743109449583.961425386430.0924073508083-0.74368027492-0.713818640078-0.02858888066040.438387148672-1.055426718030.4204774594160.675223704333-0.4618203077530.808615221950.291058143272-0.01092146818570.71567720417-0.1409079387190.95374265434692.998-5.061-4.365
167.81275678783-1.766488031230.03956978279351.537182932490.6479757063916.23177446629-0.09817752584090.2631579396281.315800677810.452967957576-0.0253966319525-1.626232036620.1025804389910.7203522733690.1598868365060.9769837405540.0705963015959-0.09856510108320.6170645417-0.07196375040161.751337838889.7683.184-40.056
171.61479783862-2.432211659260.9544306294643.63355870002-1.103610036763.46605109181-0.04645848328080.2423565016840.477399696389-0.05800023873810.03347025725990.358532013983-0.2404980536210.04069647117170.08159091577410.804932459025-0.1937237262130.0764394050561.01052100407-0.2126588019262.22366967434122.218-12.027-33.334
185.103270461060.2176027311430.4292287171891.57116203069-0.7151427092242.86616457456-0.5395514612820.01243295806-0.3460093007580.3155017687020.235717649163-1.000271683510.02389175119540.3883716834590.2575406889110.7863104255130.152930497633-0.03201163449290.809586446762-0.2868060928381.92570268726129.067-25.267-15.914
192.88712653179-1.486111387212.274560997713.42247159449-2.892167279265.403084737250.213525922717-0.235556494539-0.950974181048-0.1693572545580.0566255563068-0.1452044853780.7166673291260.0130926760127-0.2986890044210.8099664697620.09440676857590.1186232520370.597469266719-0.1469762985751.2650598589784.78-20.384-15.592
202.34126266633-1.617849544320.1150931756721.13191524813-0.2742183457760.419620697837-0.913481844691-0.259533396277-0.8621690004830.280616527190.664901051687-0.731339451536-0.593852024950.1033005248480.2212956777020.999283327610.157951008898-0.04908094018490.852550398798-0.2175587863711.55905317207118.394-10.566-12.21
214.2868667965-1.18890613251-1.729400249475.073512352061.578406850515.23930041396-0.6046661865961.04940865424-0.467169871094-1.248049512330.0526607328948-0.77845273718-0.09276578059591.092801146760.529813171820.712948921222-0.03963088517110.2660597258780.8623093527640.02655577298930.58993330030879.28126.72-36.616
223.35459778028-1.373804604150.4532494304363.837740929552.262419992994.070017160380.05019848905550.291559330544-0.755955828620.260224301622-0.237462057454-0.1827221235681.03897614867-0.8442384883050.1722484001270.713984174133-0.2527239506640.09799277855330.516192146148-0.04279197125820.68879513652452.75-12.875-24.732
236.30415314113-0.351697497811.03993717663.971732021840.6929221188692.84591091099-0.01815749907340.109279689973-0.3007273097230.182422282137-0.1662933107620.4340313772180.366474341281-0.4709427342820.1330919724210.50382550022-0.1459710631820.125869330940.456738042167-0.04260417574130.44401960982749.33512.84813.519
245.124865724381.72983119911.371567420145.697465597520.3032720347754.641498963190.0753805307662-0.0057607316004-0.363179412393-0.2024230525880.1103374053910.08276636344140.168357693198-0.10209261673-0.125738730560.4360078949890.00971131195940.07894502016310.3764257388290.002627139026070.23867605994861.22718.231-20.332
252.083851601020.421379747727-0.02556013811233.352809357581.353960493324.318563786330.0294295988021-0.0700981144002-0.103593323881-0.2091223292570.031737014639-0.531172473661-0.1416865485990.404056246015-0.02470546188790.2420215595960.05449350515630.05538091792570.422009530176-0.003479094621280.36481155550276.44424.367.003
265.337979323550.6777989659911.213959327744.31079705223-0.2528979009340.604125315725-0.201828026746-0.9575218356470.03322697174510.214361715054-0.0009999427382061.603049679660.00460540949216-2.145738882080.1898244147960.929420181859-0.03969414160850.0002406603127222.59275848548-0.2464353350771.6272989699922.5237.67-14.846
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN E AND RESID 24:127 )E24 - 127
2X-RAY DIFFRACTION2( CHAIN E AND RESID 128:228 )E128 - 228
3X-RAY DIFFRACTION3( CHAIN E AND RESID 229:349 )E229 - 349
4X-RAY DIFFRACTION4( CHAIN E AND RESID 350:449 )E350 - 449
5X-RAY DIFFRACTION5( CHAIN E AND RESID 450:558 )E450 - 558
6X-RAY DIFFRACTION6( CHAIN E AND RESID 559:599 ) OR ( CHAIN A AND RESID 750:827 )E559 - 599
7X-RAY DIFFRACTION6( CHAIN E AND RESID 559:599 ) OR ( CHAIN A AND RESID 750:827 )A750 - 827
8X-RAY DIFFRACTION7( CHAIN E AND RESID 600:663 )E600 - 663
9X-RAY DIFFRACTION8( CHAIN A AND RESID 672:742 )A672 - 742
10X-RAY DIFFRACTION9( CHAIN A AND RESID 828:933 )A828 - 933
11X-RAY DIFFRACTION10( CHAIN A AND ( RESID 934:985 OR RESID 1290:1355 ) )A934 - 985
12X-RAY DIFFRACTION10( CHAIN A AND ( RESID 934:985 OR RESID 1290:1355 ) )A1290 - 1355
13X-RAY DIFFRACTION11( CHAIN A AND RESID 1356:1494 )A1356 - 1494
14X-RAY DIFFRACTION12( CHAIN A AND RESID 986:1289 )A986 - 1289
15X-RAY DIFFRACTION13( CHAIN A AND RESID 1495:1661 )A1495 - 1661
16X-RAY DIFFRACTION14( CHAIN F AND RESID 24:127 )F24 - 127
17X-RAY DIFFRACTION15( CHAIN F AND RESID 128:228 )F128 - 228
18X-RAY DIFFRACTION16( CHAIN F AND RESID 229:349 )F229 - 349
19X-RAY DIFFRACTION17( CHAIN F AND RESID 350:449 )F350 - 449
20X-RAY DIFFRACTION18( CHAIN F AND RESID 450:558 )F450 - 558
21X-RAY DIFFRACTION19( CHAIN F AND RESID 559:599 ) OR ( CHAIN B AND RESID 750:827 )F559 - 599
22X-RAY DIFFRACTION19( CHAIN F AND RESID 559:599 ) OR ( CHAIN B AND RESID 750:827 )B750 - 827
23X-RAY DIFFRACTION20( CHAIN F AND RESID 600:662 )F600 - 662
24X-RAY DIFFRACTION21( CHAIN B AND RESID 672:741 )B672 - 741
25X-RAY DIFFRACTION22( CHAIN B AND RESID 828:933 )B828 - 933
26X-RAY DIFFRACTION23( CHAIN B AND ( RESID 934:985 OR RESID 1290:1355 ) )B934 - 985
27X-RAY DIFFRACTION23( CHAIN B AND ( RESID 934:985 OR RESID 1290:1355 ) )B1290 - 1355
28X-RAY DIFFRACTION24( CHAIN B AND RESID 1356:1494 )B1356 - 1494
29X-RAY DIFFRACTION25( CHAIN B AND RESID 986:1289 )B986 - 1289
30X-RAY DIFFRACTION26( CHAIN B AND RESID 1495:1661 )B1495 - 1661

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