+Open data
-Basic information
Entry | Database: PDB / ID: 8ca2 | ||||||
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Title | ENGINEERING THE HYDROPHOBIC POCKET OF CARBONIC ANHYDRASE II | ||||||
Components | CARBONIC ANHYDRASE IICarbonic anhydrase | ||||||
Keywords | LYASE(OXO-ACID) | ||||||
Function / homology | Function and homology information positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway ...positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway / positive regulation of synaptic transmission, GABAergic / morphogenesis of an epithelium / regulation of intracellular pH / carbonic anhydrase / carbonate dehydratase activity / carbon dioxide transport / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / neuron cellular homeostasis / one-carbon metabolic process / apical part of cell / myelin sheath / extracellular exosome / zinc ion binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Alexander, R.S. / Christianson, D.W. | ||||||
Citation | Journal: Biochemistry / Year: 1991 Title: Engineering the hydrophobic pocket of carbonic anhydrase II. Authors: Alexander, R.S. / Nair, S.K. / Christianson, D.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ca2.cif.gz | 61 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ca2.ent.gz | 44.1 KB | Display | PDB format |
PDBx/mmJSON format | 8ca2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ca/8ca2 ftp://data.pdbj.org/pub/pdb/validation_reports/ca/8ca2 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES 30 AND 202 ARE CIS-PROLINES. |
-Components
#1: Protein | Mass: 29328.078 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / References: UniProt: P00918, carbonic anhydrase |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-HG / |
#4: Water | ChemComp-HOH / |
Compound details | SECONDARY STRUCTURE ELEMENTS WERE DEFINED USING THE PROGRAM *DSSP* (W. KABSCH, C. SANDER, ...SECONDARY STRUCTURE ELEMENTS WERE DEFINED USING THE PROGRAM *DSSP* (W. KABSCH, C. SANDER, BIOPOLYMER |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.61 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 6.5 Å / Num. obs: 8367 / Num. measured all: 18465 / Rmerge F obs: 0.085 |
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-Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||
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Refinement | Rfactor obs: 0.17 / Highest resolution: 2.4 Å | |||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.4 Å
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Software | *PLUS Name: PROLSQ / Classification: refinement | |||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 6.5 Å / Num. reflection obs: 6990 / Rfactor obs: 0.176 | |||||||||||||||
Solvent computation | *PLUS | |||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||
Refine LS restraints | *PLUS
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