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Yorodumi- PDB-8c4a: Structural and interactional insights into the glideosome-associa... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8c4a | ||||||
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Title | Structural and interactional insights into the glideosome-associated connector from Toxoplasma gondii | ||||||
Components | Putative anonymous antigen-1 | ||||||
Keywords | STRUCTURAL PROTEIN / glideosome-associated connector (GAC) / Toxoplasma gondii | ||||||
Function / homology | Armadillo/plakoglobin ARM repeat profile. / Armadillo/beta-catenin-like repeats / Armadillo / Armadillo-like helical / Armadillo-type fold / Putative anonymous antigen-1 Function and homology information | ||||||
Biological species | Toxoplasma gondii (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.675 Å | ||||||
Authors | Kumar, A. / Morgan, R.M.L. / Matthews, S.J. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Elife / Year: 2023 Title: Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii. Authors: Kumar, A. / Vadas, O. / Dos Santos Pacheco, N. / Zhang, X. / Chao, K. / Darvill, N. / Rasmussen, H.O. / Xu, Y. / Lin, G.M. / Stylianou, F.A. / Pedersen, J.S. / Rouse, S.L. / Morgan, M.L. / ...Authors: Kumar, A. / Vadas, O. / Dos Santos Pacheco, N. / Zhang, X. / Chao, K. / Darvill, N. / Rasmussen, H.O. / Xu, Y. / Lin, G.M. / Stylianou, F.A. / Pedersen, J.S. / Rouse, S.L. / Morgan, M.L. / Soldati-Favre, D. / Matthews, S. #1: Journal: To Be Published Title: Structural and interactional insights into the glideosome-associated connector from Toxoplasma gondii Authors: Kumar, A. / Morgan, M.R. / Matthews, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8c4a.cif.gz | 853.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8c4a.ent.gz | 725.6 KB | Display | PDB format |
PDBx/mmJSON format | 8c4a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c4/8c4a ftp://data.pdbj.org/pub/pdb/validation_reports/c4/8c4a | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 274375.750 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Toxoplasma gondii (eukaryote) / Gene: TGRH88_051880 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A7J6JYP1 |
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#2: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.97 Å3/Da / Density % sol: 58.62 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 5 / Details: NA |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: DIAMOND / Beamline: I04 / Wavelength: 0.9795, 0.9796, 0.9722 | ||||||||||||
Detector | Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Oct 21, 2022 | ||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.675→110.75 Å / Num. obs: 904519 / % possible obs: 99.9 % / Redundancy: 9.9 % / CC1/2: 0.993 / Net I/σ(I): 14.1 | ||||||||||||
Reflection shell | Resolution: 2.675→2.771 Å / Num. unique obs: 9144 / CC1/2: 0.681 / CC star: 0.9 / % possible all: 99.42 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.675→110.75 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.675→110.75 Å
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