PDB-8ay3: X-RAY CRYSTAL STRUCTURE OF THE CsPYL1-iSB9-HAB1 TERNARY COMPLEX

ID or keywords:

Entry

Database: PDB / ID: 8ay3

Title

X-RAY CRYSTAL STRUCTURE OF THE CsPYL1-iSB9-HAB1 TERNARY COMPLEX

Components

CsPYL1
Protein phosphatase 2C 16

Keywords

PLANT PROTEIN / CsPYL1 / HAB1 / Phosphatase ABA receptor inhibitor complex

Function / homology

Function and homology information

abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / myosin phosphatase activity /

protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase /

signaling receptor activity /

metal ion binding /

nucleus /

plasma membrane /

cytoplasm
Similarity search - Function

Biological species

Citrus sinensis (sweet orange)

Arabidopsis thaliana (thale cress)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

FOURIER SYNTHESIS / Resolution: 1.9 Å

Authors

Infantes, L. / Albert, A.

Funding support

Spain, 1items

Citation

Journal: Sci Adv / Year: 2023
Title: Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought.
Authors: Lozano-Juste, J. / Infantes, L. / Garcia-Maquilon, I. / Ruiz-Partida, R. / Merilo, E. / Benavente, J.L. / Velazquez-Campoy, A. / Coego, A. / Bono, M. / Forment, J. / Pampin, B. / Destito, P. ...

History

Deposition	Sep 1, 2022	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Mar 22, 2023	Provider: repository / Type: Initial release
Revision 1.1	Feb 7, 2024	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8ay3.cif.gz	258.3 KB	Display	PDBx/mmCIF format
PDB format	pdb8ay3.ent.gz	170.5 KB	Display	PDB format
PDBx/mmJSON format	8ay3.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/ay/8ay3 ftp://data.pdbj.org/pub/pdb/validation_reports/ay/8ay3	HTTPS FTP

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Related structure data

Related structure data	6zucC 8ay6C 8ay7C 8ay8C 8ay9C 8ayaC 5mn0S 7avw C: citing same article (ref.) S: Starting model for refinement
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: CsPYL1

B: Protein phosphatase 2C 16

hetero molecules

61.1 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by software
Evidence: gel filtration

Unit cell

Length a, b, c (Å)	42.702, 62.250, 187.014
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	19
Space group name H-M	P2₁2₁2₁
Space group name Hall	P2ac2ab
Symmetry operation	#1: x,y,z #2: x+1/2,-y+1/2,-z #3: -x,y+1/2,-z+1/2 #4: -x+1/2,-y,z+1/2

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Protein , 2 types, 2 molecules AB

#1: Protein	CsPYL1 Mass: 23293.822 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Citrus sinensis (sweet orange) / Gene: CISIN_1g046151mg / Production host: Escherichia coli (E. coli) / References: UniProt: A0A067E666
#2: Protein	Protein phosphatase 2C 16 / AtPP2C16 / AtP2C-HA / Protein HYPERSENSITIVE TO ABA 1 / Protein phosphatase 2C HAB1 / PP2C HAB1 Mass: 36971.438 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: HAB1, P2C-HA, At1g72770, F28P22.4 / Production host: Escherichia coli (E. coli) References: UniProt: Q9CAJ0, protein-serine/threonine phosphatase

#1: Protein

CsPYL1

Mass: 23293.822 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Citrus sinensis (sweet orange) / Gene: CISIN_1g046151mg / Production host:

Escherichia coli (E. coli) / References: UniProt: A0A067E666

#2: Protein

Protein phosphatase 2C 16 / AtPP2C16 / AtP2C-HA / Protein HYPERSENSITIVE TO ABA 1 / Protein phosphatase 2C HAB1 / PP2C HAB1

Mass: 36971.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Arabidopsis thaliana (thale cress) / Gene: HAB1, P2C-HA, At1g72770, F28P22.4 / Production host:

Escherichia coli (E. coli)
References: UniProt: Q9CAJ0, protein-serine/threonine phosphatase

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Non-polymers , 5 types, 177 molecules

#3: Chemical	ChemComp-OE3 / ~{N}-[(1-ethyl-4-methyl-2-oxidanylidene-quinolin-6-yl)methyl]benzenesulfonamide Mass: 356.439 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₁₉H₂₀N₂O₃S / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mn
#5: Chemical	ChemComp-CL / CHLORIDE ION / Chloride Mass: 35.453 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Cl
#6: Chemical	ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₃H₈O₃
#7: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 165 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.06 Å³/Da / Density % sol: 40.36 %
Crystal grow	Temperature: 291 K / Method: microbatch / pH: 6.5 / Details: 30% PEG3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.979257 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 30, 2019
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.979257 Å / Relative weight: 1
Reflection	Resolution: 1.9→44.05 Å / Num. obs: 39950 / % possible obs: 98.92 % / Redundancy: 6.4 % / B_iso Wilson estimate: 35.17 Å² / CC_1/2: 0.999 / R_merge(I) obs: 0.09521 / R_rim(I) all: 0.104 / Net I/σ(I): 11.48
Reflection shell	Resolution: 1.9→1.968 Å / R_merge(I) obs: 2.313 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 3884 / CC_1/2: 0.502 / CC star: 0.817 / R_rim(I) all: 2.525 / % possible all: 98.25

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Processing

Software

Name	Version	Classification
XDS	2019	data reduction
XDS	2019	data scaling
PHENIX	1.19.2-4158	phasing
PHENIX	1.19.2-4158	refinement

Refinement

Method to determine structure:

FOURIER SYNTHESIS
Starting model: 5MN0

5mn0

Resolution: 1.9→44.05 Å / SU ML: 0.2671 / Cross valid method: FREE R-VALUE / σ(F): 0.2 / Phase error: 29.4101
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2409	3663	4.9 %
R_work	0.1944	71146	-
obs	0.1967	39950	98.03 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 49.86 Å²

Refinement step

Cycle: LAST / Resolution: 1.9→44.05 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3809	0	41	165	4015

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0084	3911
X-RAY DIFFRACTION	f_angle_d	0.9294	5291
X-RAY DIFFRACTION	f_chiral_restr	0.0599	592
X-RAY DIFFRACTION	f_plane_restr	0.0055	708
X-RAY DIFFRACTION	f_dihedral_angle_d	21.6378	1450

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.9-1.92	0.3973	149	0.3996	2461	X-RAY DIFFRACTION	88.59
1.92-1.95	0.4143	155	0.3688	2720	X-RAY DIFFRACTION	97.76
1.95-1.98	0.3713	120	0.316	2768	X-RAY DIFFRACTION	98.63
1.98-2.01	0.3522	125	0.3037	2748	X-RAY DIFFRACTION	98.39
2.01-2.04	0.3412	147	0.2814	2804	X-RAY DIFFRACTION	98.47
2.04-2.07	0.4387	122	0.3013	2651	X-RAY DIFFRACTION	96.82
2.07-2.11	0.3504	141	0.2909	2726	X-RAY DIFFRACTION	97.62
2.11-2.14	0.3114	123	0.2497	2814	X-RAY DIFFRACTION	98.33
2.14-2.19	0.2857	129	0.254	2696	X-RAY DIFFRACTION	98.81
2.19-2.23	0.2708	170	0.2585	2793	X-RAY DIFFRACTION	98.41
2.23-2.28	0.3922	155	0.2976	2602	X-RAY DIFFRACTION	95.04
2.28-2.33	0.2835	147	0.2425	2735	X-RAY DIFFRACTION	98.7
2.33-2.39	0.2899	147	0.2237	2805	X-RAY DIFFRACTION	99.23
2.39-2.45	0.2869	142	0.2149	2733	X-RAY DIFFRACTION	99.1
2.45-2.53	0.255	128	0.2128	2803	X-RAY DIFFRACTION	98.55
2.53-2.61	0.2557	134	0.2045	2726	X-RAY DIFFRACTION	98.79
2.61-2.7	0.2448	158	0.2061	2774	X-RAY DIFFRACTION	98.85
2.7-2.81	0.2753	138	0.2055	2773	X-RAY DIFFRACTION	99.49
2.81-2.94	0.2521	132	0.1964	2771	X-RAY DIFFRACTION	99.11
2.94-3.09	0.2537	142	0.1801	2772	X-RAY DIFFRACTION	99.28
3.09-3.29	0.1843	152	0.1747	2778	X-RAY DIFFRACTION	99.49
3.29-3.54	0.2375	121	0.1595	2745	X-RAY DIFFRACTION	98.49
3.54-3.89	0.169	140	0.1497	2744	X-RAY DIFFRACTION	97.73
3.9-4.46	0.2331	148	0.1503	2702	X-RAY DIFFRACTION	97.24
4.46-5.61	0.1836	151	0.1618	2742	X-RAY DIFFRACTION	99.11
5.62-44.05	0.2179	147	0.1857	2760	X-RAY DIFFRACTION	98.88

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.00948934262	-0.763589470629	0.145385495706	2.50141091303	-0.176730629816	1.07938085692	0.0329591022063	-0.27814304712	-0.118427741562	0.675542821154	0.0183825211212	-0.306320312415	0.302915360739	0.283876889343	-0.0271682758164	0.68543937105	-0.0154279894495	-0.142904657852	0.416777911503	0.0568536910436	0.420934309445	8.6677483529	6.26188982942	51.4072572235
2	1.27417400356	0.144262416923	-0.708084789493	1.43923811663	-0.537064692054	2.41107308587	0.0528402213043	0.0114304894674	-0.0973143100366	0.184414158211	0.0881452500652	0.314534539601	0.547059198143	-0.211562225461	-0.129392973958	0.492451220116	-0.0366481313116	-0.0209433875304	0.312963303042	0.0182986286303	0.343312888881	-6.83668246591	1.86786145358	38.1878760377
3	2.10113019209	1.04151046111	0.35202225364	2.53795168069	-0.0136444844462	2.48715395567	0.195772092364	0.0532603307109	-0.0528861296077	0.589730657426	-0.164107347789	-0.207216373007	0.363484130985	0.0175448975604	-0.0249244920199	0.498355620634	0.00890966306722	-0.0689878105564	0.28408663521	0.0530271891279	0.324134872045	3.63370617891	6.55273986656	42.9734531369
4	6.01160374896	0.314117493655	-2.46099851672	2.3472327915	0.0873878405345	2.82195850169	0.253428930959	0.0834756920946	0.816555912032	0.202940174073	0.0884956647739	-0.198366442137	0.0274040586531	0.0908993637203	-0.356393977023	0.445322523066	0.0174506164277	-0.0224105009963	0.340541799497	-0.00533693010573	0.356017892539	1.94538122881	13.6466521918	39.4162020047
5	5.28640433696	-0.729607853962	-0.725897596216	5.80705401653	0.719872897644	5.8051384793	-0.024946931747	-0.20567697839	0.635701199689	0.0818895531177	0.0635139221865	0.00883983087565	-0.660444690675	0.371206723226	0.023582468373	0.329073101784	0.0529801305177	-0.0472718309123	0.322779075811	-0.0135688470714	0.368846639874	-7.08629491499	32.6998474321	-4.67625876925
6	1.70830584858	1.17130393073	-0.703005273069	3.76000927788	-3.75931336434	6.0722514642	0.0584262100208	0.0233970609883	0.0853081215469	-0.172714645378	0.204192189939	-0.173095779537	0.234072510216	0.210026751051	-0.0577867322537	0.17009429734	0.00931234154024	-0.0018509522634	0.260993404977	0.0174836210323	0.287131281518	-6.2867844233	18.0153560031	2.80941412417
7	4.57450612322	-0.247189276094	1.14245135498	4.74187506952	-1.94427661534	8.90768451342	-0.0348141456722	-0.284970171723	-0.460764099462	0.731297662378	0.287998930691	-0.384574767627	0.803037694592	0.216545560769	-0.0666546315201	0.394822743418	0.0205378868699	-0.0235894050228	0.287598160633	0.0437939077244	0.366098581612	-4.63873952052	7.75384020809	12.1100836068
8	2.13278327303	-2.03697579489	1.32816774478	4.84423554881	-1.9902274619	3.79013570026	-0.177700490296	0.0461635429236	0.265738100359	-0.142383552637	-0.046713664215	-0.0274805307947	0.258182166931	-0.341716282795	0.173950425979	0.177271252738	0.00976391405371	0.0326618511468	0.300045684912	-0.0131771188761	0.2958572397	-8.31036277521	21.2389145396	10.049057608
9	0.855559445833	1.80378423714	-0.338596913772	6.57132014661	2.08006269001	2.88826252631	-0.0306189337378	-0.148773446444	0.423870257223	0.218961262366	0.925194660315	0.0487827085886	-0.703041193255	0.269387739874	-0.6278031536	0.38011293935	0.0917017082705	0.0429226268293	0.509470705609	-0.0150990309394	0.490652410613	-10.2173328897	40.7234931243	4.42272605605
10	1.33023441278	-1.15163896588	0.910243603913	3.73380609604	-0.505980819021	0.641414932757	-0.0748412306709	-0.070603907855	0.720516982333	0.950743573314	-0.531148338727	0.286927184072	0.0611378361654	-0.57531695345	0.214221416639	0.697923095935	-0.176041051193	0.24373097553	0.432894729418	-0.139742193421	0.848360847228	-1.07886719809	49.602063327	4.86826787116
11	1.95047869113	0.683366308588	-0.381976779126	2.91861461794	-2.55688000685	3.46718653995	0.138820897739	0.177815754742	0.195603174579	0.1652310511	0.0293752242937	0.216651297816	-0.311611007299	-0.0884206352004	-0.107952224581	0.251169565916	0.059509636718	0.0395155824182	0.266182673553	0.00188364712477	0.270571856971	-5.61307343922	29.3862187243	9.63818908573
12	1.93231616014	-0.0010066761677	-0.250634560955	4.71149816788	-0.872876806028	2.15567956811	0.0474175806024	-0.0605444325092	0.338557266374	0.28248356778	0.0854065537356	0.0989523330956	-0.413971300302	-0.0613020013313	-0.179596124792	0.401735687307	0.0176633109024	0.0530018953156	0.364655004228	-0.0175010771229	0.393578305271	-9.73133076492	36.4266455503	15.8846634346
13	3.07813171876	0.965473564851	-0.843051418989	3.74605046206	-1.04743800447	3.69221700069	-0.0739222919133	-0.0297475523256	0.0630998056243	0.378972672256	0.114919286103	-0.465422290653	-0.469029751599	0.177733414438	-0.0993668015586	0.393762723453	0.00874598431433	0.00651514703016	0.305417634994	-0.100390711729	0.334579135899	-1.41859523859	37.3284838049	19.0997585858
14	2.35190261512	0.561317396008	-2.95139072117	7.71583606468	0.372697739233	3.80388909843	-0.0266560108056	-0.012807718615	-0.0130434196882	-0.0601602319578	0.0448908618245	0.30743191702	0.017758698971	-0.647642519105	-0.103053916453	1.04788757703	0.170750784325	-0.0622930525804	0.787567294229	0.0783641816037	0.629690726862	-12.4372664047	32.6421334425	28.0040733073
15	0.802103642358	-0.286719910757	0.795523464226	1.02659957175	0.128213185234	1.36951242158	0.206902958807	-0.294203113041	0.201799993238	0.48290380522	0.481339087527	1.19128928628	-0.282887759291	-0.503667158993	-0.601333154806	0.406514953857	0.0835758954311	0.176365573586	0.372780822934	0.103686562254	0.562346513537	-7.22945788775	29.4972514689	19.0523633866
16	0.0896323517972	-0.621425158758	-0.132873466512	0.609376443505	-0.120688749937	1.5337569778	0.00857575113898	-0.0650006161127	0.0768811070988	0.0438602964292	0.0613298170811	-0.101662522571	-0.123450478937	0.166558894084	-0.0398202797395	0.293616460825	-0.0528898940568	0.00721496971133	0.329300437403	-0.00709485258195	0.325822772178	8.68900383586	28.5585550508	7.80693261411
17	6.44943689532	-2.0798595783	-0.700749821947	5.7133963123	0.325007199848	7.26324582321	0.0719791217636	-0.551765612532	0.109432187167	0.0879232441425	-0.224757607632	-0.651161670297	-0.385203426014	1.13885285344	-0.118038066048	0.41106931917	-0.084063365443	0.0161238933848	0.494950864615	-0.00283588344448	0.42857473747	15.5054240158	27.3181755072	26.6000026493
18	2.48119129526	0.025602069633	0.973182702255	4.35560879158	0.0053718504984	1.32436082929	0.0227898482212	-0.178381266041	-0.128638114389	-0.0331340311951	0.166774515985	-0.396915718667	-0.0439077870842	0.193314305846	-0.113271085017	0.282318331505	-0.021062032193	-0.0051334893522	0.425144872035	-0.0236050381267	0.238760572708	8.58459487239	18.5075982779	25.8454969572
19	2.43798481564	0.279752614699	0.0286517255826	1.77491819758	-0.523038874964	5.50070418545	-0.131407310704	-0.356418786422	0.411750989468	0.541599017908	-0.00277063084174	0.130382478193	-0.0551122854554	-0.12468030849	0.154618457562	0.389679907277	0.00754252303016	-0.027469075039	0.349762200788	-0.0600043351928	0.318454482415	2.86334610966	27.1305431372	28.1922239185
20	1.32160388458	0.408984216198	-0.276940257168	1.47942770074	0.346539245015	9.71825274376	0.110951963433	-0.0659850950733	0.328840711538	-0.229666652273	-0.173574863438	-0.116932067864	-0.665939132097	1.08777450497	0.037941545531	0.405040630903	-0.0940274766476	0.0604934204581	0.415767919396	0.0165570870517	0.376721673878	7.24671043136	38.2223147246	1.44327002052
21	1.05926755553	-0.483571913232	0.0980680079891	2.54343816222	-1.42778517817	1.37265371509	0.0802313049379	-0.086957226832	-0.0224224508108	-0.410295866893	-0.0439085719477	0.101537620242	0.201276951875	-0.0240618589265	-0.0568514365695	0.232841475926	-0.00817394874252	0.009575612757	0.319588641474	0.0193453252152	0.322826133444	5.37036757599	21.563168936	2.95594558843
22	4.95044908545	0.0844996832174	1.99121647295	1.79225744781	-0.135491997669	3.87062783791	-0.0239012229704	0.225715018876	-0.0443103407876	-0.203986979949	-0.0133634395106	-0.227950242099	0.297372979601	-0.00121354458002	-0.0331691903541	0.189448871637	0.00214435256263	0.0263820444041	0.229899670606	0.00629099118833	0.243844921382	3.51805145025	21.2438093728	-10.1653992301
23	0.436513823053	-0.854948824938	0.851412468973	1.73505783869	-1.79137654883	1.78314814028	0.774840742216	0.259569056395	0.222671905622	-1.40865462306	0.0438318926642	1.05871940123	-0.427963061634	-0.368787644283	-0.48725356602	0.611502398206	0.0284558242911	-0.0283620714201	0.37718055222	0.0649055588669	0.57807908753	-11.4523076115	29.7979661412	-11.3773307781
24	1.43377730223	-2.32575297538	1.23332504874	3.88324315036	-2.51304785792	1.94360994328	0.0432857139069	-0.171627714104	-0.263278266535	-0.229350296563	0.12337443343	0.393854782942	0.167377792955	-0.15476287934	-0.15192902255	0.235696950928	-0.0414981032232	-0.0038543436752	0.328976257954	-0.00154570553901	0.328779244186	-1.35564510454	15.1918893582	-3.60831692954
25	1.85864304614	0.246832374067	-0.941196041417	1.71146555555	0.477836150316	1.56494678443	-0.153513013754	0.0844967030754	0.0585676535239	-0.359099605174	0.140542279417	0.0181087568505	0.170389764661	-0.0705719484556	0.0248727713393	0.193827382736	-0.0134301038611	-0.00996851967839	0.277453276586	0.0118520470899	0.277098567613	0.420708663277	21.671818775	-0.600549659114

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 21 through 67 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 68 through 141 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 142 through 181 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 182 through 207 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 186 through 190 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 191 through 195 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 196 through 200 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 201 through 210 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 211 through 215 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 216 through 221 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 233 through 248 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 249 through 273 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 283 through 302 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 303 through 308 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 314 through 337 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 338 through 368 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 369 through 378 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 379 through 402 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 403 through 413 )
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 414 through 423 )
21	X-RAY DIFFRACTION	21	chain 'B' and (resid 424 through 439 )
22	X-RAY DIFFRACTION	22	chain 'B' and (resid 440 through 458 )
23	X-RAY DIFFRACTION	23	chain 'B' and (resid 459 through 471 )
24	X-RAY DIFFRACTION	24	chain 'B' and (resid 472 through 487 )
25	X-RAY DIFFRACTION	25	chain 'B' and (resid 488 through 505 )

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