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- PDB-7yof: Crystal Structure of Triple mutant (D67E, A68P, I88L) of O-acetyl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7yof | ||||||
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Title | Crystal Structure of Triple mutant (D67E, A68P, I88L) of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae at 2.3 A | ||||||
![]() | Cysteine synthase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kumar, N. / Rahisuddin, R. / Saini, N. / Singh, A.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal Structure of I88L single mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae at 2.3 A Authors: Kumar, N. / Rahisuddin, R. / Saini, N. / Singh, A.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.3 KB | Display | ![]() |
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PDB format | ![]() | 52.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7yogC ![]() 1y7lS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 33691.527 Da / Num. of mol.: 1 / Mutation: D67E, A68P, I88L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: cysK, HI_1103 / Production host: ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 41.06 % |
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Crystal grow![]() | Temperature: 278 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1M HEPES, 1.3M sodium citrate / PH range: 7.3-7.9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Aug 27, 2014 |
Radiation | Monochromator: Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.3→40.75 Å / Num. obs: 235300 / % possible obs: 99.98 % / Redundancy: 19.1 % / CC1/2: 0.847 / CC star: 0.958 / Net I/σ(I): 66.54 |
Reflection shell | Resolution: 2.3→2.382 Å / Mean I/σ(I) obs: 13.29 / Num. unique obs: 1178 / CC1/2: 0.781 / CC star: 0.936 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1Y7L Resolution: 2.3→40.75 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.915 / SU B: 6.273 / SU ML: 0.151 / Cross valid method: FREE R-VALUE / ESU R: 0.406 / ESU R Free: 0.233 Details: Hydrogens have been used if present in the input file
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.432 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→40.75 Å
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Refine LS restraints |
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LS refinement shell |
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