+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 7xqd | ||||||
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タイトル | Structure of C-terminal truncated connexin43/Cx43/GJA1 gap junction intercellular channel in POPE/CHS nanodiscs (C1 symmetry) | ||||||
要素 | Gap junction alpha-1 protein | ||||||
キーワード | MEMBRANE PROTEIN (膜タンパク質) / Cx43 (コネキシン43) / Connexin (コネクシン) / Gap junction channel / Gating mechanism | ||||||
機能・相同性 | 機能・相同性情報 gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / 授乳 / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration ...gap junction channel activity involved in cardiac conduction electrical coupling / negative regulation of gonadotropin secretion / positive regulation of striated muscle tissue development / 授乳 / positive regulation of morphogenesis of an epithelium / positive regulation of mesodermal cell differentiation / atrial ventricular junction remodeling / cell communication by chemical coupling / cell communication by electrical coupling / neuroblast migration / gap junction hemi-channel activity / columnar/cuboidal epithelial cell maturation / negative regulation of trophoblast cell migration / regulation of bone remodeling / microtubule-based transport / monoatomic ion transmembrane transporter activity / SARS-CoV-2 targets PDZ proteins in cell-cell junction / glutathione transmembrane transporter activity / regulation of ventricular cardiac muscle cell membrane depolarization / gap junction channel activity involved in cell communication by electrical coupling / contractile muscle fiber / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane / gap junction assembly / cellular response to pH / atrial cardiac muscle cell action potential / Regulation of gap junction activity / skeletal muscle tissue regeneration / cardiac conduction system development / connexin complex / regulation of atrial cardiac muscle cell membrane depolarization / Formation of annular gap junctions / Golgi-associated vesicle membrane / Gap junction degradation / fascia adherens / bone remodeling / cell-cell contact zone / Gap junction assembly / gap junction channel activity / ギャップ結合 / export across plasma membrane / adult heart development / regulation of bone mineralization / glutamate secretion / xenobiotic transport / regulation of ventricular cardiac muscle cell membrane repolarization / 密着結合 / blood vessel morphogenesis / lens development in camera-type eye / cell communication by electrical coupling involved in cardiac conduction / 中間径フィラメント / embryonic digit morphogenesis / maintenance of blood-brain barrier / beta-tubulin binding / heart looping / positive regulation of stem cell proliferation / RHOJ GTPase cycle / RHOQ GTPase cycle / efflux transmembrane transporter activity / establishment of mitotic spindle orientation / 介在板 / alpha-tubulin binding / lateral plasma membrane / T cell proliferation / monoatomic ion transmembrane transport / positive regulation of vascular associated smooth muscle cell proliferation / tubulin binding / protein tyrosine kinase binding / neuron migration / 骨 / protein localization / negative regulation of cell growth / beta-catenin binding / osteoblast differentiation / cellular response to amyloid-beta / male gonad development / 細胞結合 / cell-cell signaling / positive regulation of cold-induced thermogenesis / heart development / scaffold protein binding / 精子形成 / positive regulation of canonical NF-kappaB signal transduction / in utero embryonic development / 脂質ラフト / apical plasma membrane / ゴルジ体 / negative regulation of gene expression / signaling receptor binding / intracellular membrane-bounded organelle / focal adhesion / endoplasmic reticulum membrane / positive regulation of gene expression / ゴルジ体 / シグナル伝達 / ミトコンドリア / 核質 / 細胞核 / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.7 Å | ||||||
データ登録者 | Lee, H.J. / Cha, H.J. / Jeong, H. / Lee, S.N. / Lee, C.W. / Woo, J.S. | ||||||
資金援助 | 韓国, 1件
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引用 | ジャーナル: Nat Commun / 年: 2023 タイトル: Conformational changes in the human Cx43/GJA1 gap junction channel visualized using cryo-EM. 著者: Hyuk-Joon Lee / Hyung Jin Cha / Hyeongseop Jeong / Seu-Na Lee / Chang-Won Lee / Minsoo Kim / Jejoong Yoo / Jae-Sung Woo / 要旨: Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). ...Connexin family proteins assemble into hexameric hemichannels in the cell membrane. The hemichannels dock together between two adjacent membranes to form gap junction intercellular channels (GJIChs). We report the cryo-electron microscopy structures of Cx43 GJICh, revealing the dynamic equilibrium state of various channel conformations in detergents and lipid nanodiscs. We identify three different N-terminal helix conformations of Cx43-gate-covering (GCN), pore-lining (PLN), and flexible intermediate (FIN)-that are randomly distributed in purified GJICh particles. The conformational equilibrium shifts to GCN by cholesteryl hemisuccinates and to PLN by C-terminal truncations and at varying pH. While GJIChs that mainly comprise GCN protomers are occluded by lipids, those containing conformationally heterogeneous protomers show markedly different pore sizes. We observe an α-to-π-helix transition in the first transmembrane helix, which creates a side opening to the membrane in the FIN and PLN conformations. This study provides basic structural information to understand the mechanisms of action and regulation of Cx43 GJICh. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7xqd.cif.gz | 1.2 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7xqd.ent.gz | 985.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7xqd.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xq/7xqd ftp://data.pdbj.org/pub/pdb/validation_reports/xq/7xqd | HTTPS FTP |
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-関連構造データ
関連構造データ | 33393MC 7f92C 7f93C 7f94C 7xq9C 7xqbC 7xqfC 7xqgC 7xqhC 7xqiC 7xqjC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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-要素
#1: タンパク質 | 分子量: 29396.547 Da / 分子数: 12 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GJA1, GJAL / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P17302 #2: 化合物 | ChemComp-C14 / #3: 化合物 | ChemComp-PTY / #4: 化合物 | ChemComp-Y01 / #5: 水 | ChemComp-HOH / | 研究の焦点であるリガンドがあるか | Y | |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Dodecameric complex of C-terminal deletion mutant of human Cx43/GJA1 (Cx43-M257) in lipid nanodiscs (POPE/CHS) タイプ: COMPLEX 詳細: Human Cx43-M257 gap junction channel with gate-covering NTH conformation Entity ID: #1 / 由来: RECOMBINANT |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Homo sapiens (ヒト) |
緩衝液 | pH: 8 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELDBright-field microscopy / 最大 デフォーカス(公称値): 2400 nm / 最小 デフォーカス(公称値): 900 nm |
撮影 | 電子線照射量: 72.42 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
-解析
ソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3次元再構成 | 解像度: 2.7 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 325564 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
精密化 | 交差検証法: NONE | ||||||||||||||||||||||||
拘束条件 |
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