+Open data
-Basic information
Entry | Database: PDB / ID: 7xce | ||||||
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Title | Crystal structure of Ankyrin G in complex with neurofascin | ||||||
Components | Neurofascin,Ankyrin-3 | ||||||
Keywords | PROTEIN BINDING / CELL ADHESION / STRUCTURAL PROTEIN | ||||||
Function / homology | Function and homology information Schwann cell microvillus / positive regulation of sodium ion import across plasma membrane / Neurofascin interactions / positive regulation of cell communication by electrical coupling / positive regulation of membrane depolarization during cardiac muscle cell action potential / regulation of protein targeting / maintenance of protein location in plasma membrane / membrane assembly / protein localization to axon / clustering of voltage-gated sodium channels ...Schwann cell microvillus / positive regulation of sodium ion import across plasma membrane / Neurofascin interactions / positive regulation of cell communication by electrical coupling / positive regulation of membrane depolarization during cardiac muscle cell action potential / regulation of protein targeting / maintenance of protein location in plasma membrane / membrane assembly / protein localization to axon / clustering of voltage-gated sodium channels / protein binding involved in heterotypic cell-cell adhesion / positive regulation of sodium ion transmembrane transporter activity / protein localization to paranode region of axon / establishment or maintenance of microtubule cytoskeleton polarity / regulation of potassium ion transport / spectrin-associated cytoskeleton / magnesium ion homeostasis / paranodal junction assembly / phosphorylation-dependent protein binding / cell-cell adhesion mediator activity / positive regulation of membrane potential / protein localization to juxtaparanode region of axon / paranodal junction / plasma membrane organization / negative regulation of delayed rectifier potassium channel activity / positive regulation of cation channel activity / positive regulation of homotypic cell-cell adhesion / axon initial segment / maintenance of protein location in cell / Golgi to plasma membrane protein transport / paranode region of axon / positive regulation of sodium ion transport / peripheral nervous system development / negative regulation of endocytosis / node of Ranvier / costamere / anterograde axonal transport / cellular response to magnesium ion / Neutrophil degranulation / spectrin binding / neuromuscular junction development / axon development / transmission of nerve impulse / plasma membrane => GO:0005886 / neuronal action potential / mitotic cytokinesis / response to immobilization stress / positive regulation of protein targeting to membrane / intercalated disc / lateral plasma membrane / axon cytoplasm / T-tubule / myelination / cytoskeletal protein binding / axonogenesis / basal plasma membrane / sarcoplasmic reticulum / axon guidance / protein localization to plasma membrane / synapse organization / brain development / sarcolemma / neuromuscular junction / establishment of protein localization / structural constituent of cytoskeleton / cell-cell adhesion / Z disc / positive regulation of non-canonical NF-kappaB signal transduction / protein-macromolecule adaptor activity / postsynaptic membrane / basolateral plasma membrane / RNA polymerase II-specific DNA-binding transcription factor binding / transmembrane transporter binding / lysosome / cytoskeleton / neuron projection / cadherin binding / axon / protein domain specific binding / dendrite / synapse / positive regulation of gene expression / regulation of transcription by RNA polymerase II / cell surface / signal transduction / membrane / nucleus / plasma membrane Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | He, L. / Li, J. / Wang, C. | ||||||
Funding support | China, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2022 Title: Crystal structure of Ankyrin-G in complex with a fragment of Neurofascin reveals binding mechanisms required for integrity of the axon initial segment. Authors: He, L. / Jiang, W. / Li, J. / Wang, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7xce.cif.gz | 60.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7xce.ent.gz | 41.3 KB | Display | PDB format |
PDBx/mmJSON format | 7xce.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xc/7xce ftp://data.pdbj.org/pub/pdb/validation_reports/xc/7xce | HTTPS FTP |
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-Related structure data
Related structure data | 5y4eS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28700.688 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: The fusion protein of Neurofascin, Linker, and Ankyrin-3 Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Nfasc, Ank3 / Production host: Escherichia coli (E. coli) / References: UniProt: P97685, UniProt: O70511 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.72 Å3/Da / Density % sol: 66.91 % |
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Crystal grow | Temperature: 289.2 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 0.1 M ammonium acetate, 0.1 M Bis-Tris (pH 5.5), 17% w/v polyethylene glycol 10000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97915 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 16, 2017 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.5→50 Å / Num. obs: 15440 / % possible obs: 99.6 % / Redundancy: 5.4 % / Rmerge(I) obs: 0.124 / Rpim(I) all: 0.059 / Rrim(I) all: 0.137 / Χ2: 2.027 / Net I/σ(I): 8.1 / Num. measured all: 83059 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5Y4E Resolution: 2.5→34.6 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 0.32 / Phase error: 22.88 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 115.15 Å2 / Biso mean: 50.9493 Å2 / Biso min: 28.42 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.5→34.6 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 5
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