+Open data
-Basic information
Entry | Database: PDB / ID: 7wju | ||||||||||||
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Title | Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex | ||||||||||||
Components |
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Keywords | RNA BINDING PROTEIN/RNA/DNA / CRISPR / targeting / RNA BINDING PROTEIN-RNA-DNA complex | ||||||||||||
Function / homology | Function and homology information endonuclease activity / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion binding Similarity search - Function | ||||||||||||
Biological species | Acidibacillus sulfuroxidans (bacteria) synthetic construct (others) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.69 Å | ||||||||||||
Authors | Wu, Z. / Liu, D. / Shen, H. / Ji, Q. | ||||||||||||
Funding support | China, 3items
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Citation | Journal: To Be Published Title: Structure-directed functional evolution of the miniature CRISPR-AsCas12f1 system Authors: Wu, Z. / Liu, D. / Pan, D. / Shen, H. / Ji, Q. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7wju.cif.gz | 194.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7wju.ent.gz | 150.3 KB | Display | PDB format |
PDBx/mmJSON format | 7wju.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wj/7wju ftp://data.pdbj.org/pub/pdb/validation_reports/wj/7wju | HTTPS FTP |
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-Related structure data
Related structure data | 32548MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 48719.742 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acidibacillus sulfuroxidans (bacteria) / Gene: BM613_13600 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2U3D0N8 #2: RNA chain | | Mass: 61643.578 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) #3: DNA chain | | Mass: 9260.985 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) #4: DNA chain | | Mass: 1840.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component |
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Source (natural) |
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Source (recombinant) |
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Buffer solution | pH: 7.5 | ||||||||||||||||||||||||||||||
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | ||||||||||||||||||||||||||||||
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1800 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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3D reconstruction | Resolution: 2.69 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1770878 / Symmetry type: POINT |