+Open data
-Basic information
Entry | Database: PDB / ID: 7vsj | ||||||
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Title | Crystal structure of MmIMP3-RRM12 in complex with 9-mer RNA | ||||||
Components |
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Keywords | RNA BINDING PROTEIN/RNA / RRM domain / RNA / complex / m6A modification / GENE REGULATION / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex | ||||||
Function / homology | Function and homology information CRD-mediated mRNA stabilization / N6-methyladenosine-containing RNA reader activity / mRNA transport / mRNA 3'-UTR binding / P-body / mRNA 5'-UTR binding / cytoplasmic stress granule / regulation of translation / nervous system development / regulation of gene expression ...CRD-mediated mRNA stabilization / N6-methyladenosine-containing RNA reader activity / mRNA transport / mRNA 3'-UTR binding / P-body / mRNA 5'-UTR binding / cytoplasmic stress granule / regulation of translation / nervous system development / regulation of gene expression / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.38 Å | ||||||
Authors | Li, X.J. / Wu, B.X. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: Crystal structure of MmIMP3-RRM12 in complex with 9-mer RNA Authors: Li, X.J. / Wu, B.X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7vsj.cif.gz | 97.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7vsj.ent.gz | 60 KB | Display | PDB format |
PDBx/mmJSON format | 7vsj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vs/7vsj ftp://data.pdbj.org/pub/pdb/validation_reports/vs/7vsj | HTTPS FTP |
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-Related structure data
Related structure data | 7eyy S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18032.477 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Igf2bp3, Vickz3 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9CPN8 |
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#2: RNA chain | Mass: 2797.775 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.48 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17B1 / Wavelength: 0.97853 Å |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Oct 23, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97853 Å / Relative weight: 1 |
Reflection | Resolution: 2.38→30 Å / Num. obs: 7733 / % possible obs: 99.9 % / Redundancy: 10.3 % / Biso Wilson estimate: 38.88 Å2 / CC1/2: 0.993 / CC star: 0.998 / Rmerge(I) obs: 0.146 / Rpim(I) all: 0.046 / Rrim(I) all: 0.153 / Net I/σ(I): 18.67 |
Reflection shell | Resolution: 2.38→2.47 Å / Redundancy: 6 % / Rmerge(I) obs: 0.731 / Mean I/σ(I) obs: 2 / Num. unique obs: 763 / CC1/2: 0.754 / CC star: 0.927 / Rpim(I) all: 0.313 / Rrim(I) all: 0.799 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7EYY 7eyy Resolution: 2.38→28.78 Å / SU ML: 0.3363 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.0591 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.64 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.38→28.78 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 19.0053424161 Å / Origin y: 14.9791187012 Å / Origin z: 11.2792972704 Å
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Refinement TLS group | Selection details: all |