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- PDB-7u0n: Crystal structure of chimeric omicron RBD (strain BA.1) complexed... -

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Basic information

Entry
Database: PDB / ID: 7u0n
TitleCrystal structure of chimeric omicron RBD (strain BA.1) complexed with human ACE2
Components
  • Angiotensin-converting enzyme 2
  • Spike protein S1
KeywordsVIRAL PROTEIN / coronavirus
Function / homology
Function and homology information


positive regulation of amino acid transport / angiotensin-converting enzyme 2 / positive regulation of L-proline import across plasma membrane / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / angiotensin-mediated drinking behavior / tryptophan transport / positive regulation of gap junction assembly / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / regulation of cardiac conduction ...positive regulation of amino acid transport / angiotensin-converting enzyme 2 / positive regulation of L-proline import across plasma membrane / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / angiotensin-mediated drinking behavior / tryptophan transport / positive regulation of gap junction assembly / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / regulation of cardiac conduction / peptidyl-dipeptidase activity / angiotensin maturation / maternal process involved in female pregnancy / Metabolism of Angiotensinogen to Angiotensins / metallocarboxypeptidase activity / Attachment and Entry / carboxypeptidase activity / negative regulation of signaling receptor activity / positive regulation of cardiac muscle contraction / regulation of cytokine production / viral life cycle / blood vessel diameter maintenance / regulation of transmembrane transporter activity / brush border membrane / negative regulation of smooth muscle cell proliferation / cilium / negative regulation of ERK1 and ERK2 cascade / endocytic vesicle membrane / metallopeptidase activity / positive regulation of reactive oxygen species metabolic process / virus receptor activity / regulation of cell population proliferation / regulation of inflammatory response / endopeptidase activity / Induction of Cell-Cell Fusion / Potential therapeutics for SARS / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / receptor-mediated virion attachment to host cell / symbiont entry into host cell / membrane raft / apical plasma membrane / endoplasmic reticulum lumen / cell surface / extracellular space / extracellular exosome / zinc ion binding / extracellular region / membrane / identical protein binding / plasma membrane
Similarity search - Function
Collectrin-like domain profile. / Collectrin domain / Renal amino acid transporter / Peptidase family M2 domain profile. / Peptidase M2, peptidyl-dipeptidase A / Angiotensin-converting enzyme / Neutral zinc metallopeptidases, zinc-binding region signature.
Similarity search - Domain/homology
Angiotensin-converting enzyme 2
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.61 Å
AuthorsGeng, Q. / Shi, K. / Ye, G. / Zhang, W. / Aihara, H. / Li, F.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01AI089728 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01AI089728 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM118047 United States
CitationJournal: J.Virol. / Year: 2022
Title: Structural Basis for Human Receptor Recognition by SARS-CoV-2 Omicron Variant BA.1.
Authors: Geng, Q. / Shi, K. / Ye, G. / Zhang, W. / Aihara, H. / Li, F.
History
DepositionFeb 18, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 30, 2022Provider: repository / Type: Initial release
Revision 1.1Apr 13, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Apr 20, 2022Group: Database references / Category: citation / Item: _citation.title
Revision 1.3May 11, 2022Group: Database references / Category: citation / Item: _citation.journal_volume
Revision 1.4Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Angiotensin-converting enzyme 2
B: Angiotensin-converting enzyme 2
E: Spike protein S1
F: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)192,72029
Polymers187,0754
Non-polymers5,64525
Water23413
1
A: Angiotensin-converting enzyme 2
E: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)95,95312
Polymers93,5372
Non-polymers2,41510
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Angiotensin-converting enzyme 2
F: Spike protein S1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)96,76717
Polymers93,5372
Non-polymers3,23015
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)81.130, 120.179, 113.325
Angle α, β, γ (deg.)90.000, 92.440, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain "A" and (resid 19 through 30 or resid 32...
21(chain "B" and (resid 19 through 30 or resid 32...
12(chain "E" and (resid 334 through 516 or resid 523 through 526))
22(chain "F" and resid 334 through 526)

NCS ensembles :
ID
1
2

NCS oper:
IDCodeMatrixVector
1given(-0.956502505759, 0.246182212283, 0.156516052951), (0.169836331876, 0.0337021504823, 0.984895824658), (0.237188905413, 0.96863743651, -0.074046875293)12.0868714918, -22.8753048394, 1.35593064008
2given(-0.979682378294, 0.191489284228, 0.0596178805987), (0.068835003894, 0.0418420552643, 0.996750211763), (0.188372449949, 0.980602415068, -0.0541730898395)17.3574036821, -22.3185896915, 1.02086634688

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Components

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Protein , 2 types, 4 molecules ABEF

#1: Protein Angiotensin-converting enzyme 2 / / ACE-related carboxypeptidase / Angiotensin-converting enzyme homolog / ACEH / Metalloprotease MPROT15


Mass: 69153.664 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ACE2, UNQ868/PRO1885 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: Q9BYF1, angiotensin-converting enzyme 2
#2: Protein Spike protein S1


Mass: 24383.633 Da / Num. of mol.: 2 / Fragment: receptor binding domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Strain: Omicron / Production host: Spodoptera frugiperda (fall armyworm)

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Sugars , 3 types, 14 molecules

#3: Polysaccharide
beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#5: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 5 types, 24 molecules

#6: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#7: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Cl
#8: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H6O2
#9: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#10: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 13 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.95 Å3/Da / Density % sol: 58.31 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop
Details: 100 mM Tris, pH 8.5, 20% PEG6000, 100 mM sodium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-1 / Wavelength: 0.979 Å
DetectorType: DECTRIS EIGER2 S 16M / Detector: PIXEL / Date: Jan 11, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.61→113 Å / Num. obs: 61585 / % possible obs: 92.6 % / Redundancy: 4 % / Biso Wilson estimate: 87.71 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.055 / Net I/σ(I): 9.3
Reflection shellResolution: 2.61→2.68 Å / Num. unique obs: 110 / CC1/2: 0.689

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDSdata reduction
Aimlessdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS
Starting model: PDB entry 2AJF

2ajf


Resolution: 2.61→30.11 Å / SU ML: 0.2981 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 33.5048
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.235 1734 4.76 %
Rwork0.1884 34727 -
obs0.1906 36461 54.88 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 93.77 Å2
Refinement stepCycle: LAST / Resolution: 2.61→30.11 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12740 0 355 13 13108
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001913453
X-RAY DIFFRACTIONf_angle_d0.557218272
X-RAY DIFFRACTIONf_chiral_restr0.06411987
X-RAY DIFFRACTIONf_plane_restr0.00332325
X-RAY DIFFRACTIONf_dihedral_angle_d10.4514907
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
12AX-RAY DIFFRACTIONTorsion NCS1.96101698646
22BX-RAY DIFFRACTIONTorsion NCS0.683059416847
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.61-2.680.903540.5749106X-RAY DIFFRACTION1.99
2.68-2.770.4743170.4255407X-RAY DIFFRACTION7.76
2.77-2.870.431420.3574714X-RAY DIFFRACTION13.79
2.87-2.980.3685720.32891127X-RAY DIFFRACTION21.71
2.98-3.120.3755980.32691657X-RAY DIFFRACTION31.94
3.12-3.280.31381200.30612449X-RAY DIFFRACTION46.68
3.28-3.490.30142230.27293729X-RAY DIFFRACTION71.59
3.49-3.760.29232500.22614699X-RAY DIFFRACTION89.33
3.76-4.130.21362070.18644682X-RAY DIFFRACTION87.81
4.13-4.730.19322210.15154989X-RAY DIFFRACTION94.38
4.73-5.950.21792300.17275055X-RAY DIFFRACTION94.8
5.95-30.110.21582500.16925113X-RAY DIFFRACTION95.11
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.402241116591.63894129775-2.411589431792.09173454467-1.402242016762.39474632021-0.397197506980.148738004629-0.788339082593-0.153441187494-0.0182766287564-0.5645158729460.721115269153-0.1403394191020.3912984288640.6629423282230.0280922047296-0.04637698494220.4313430306750.06224032481640.61484339078430.7146624454-10.642205803151.8195962921
23.750534694871.52634350857-1.461463847243.4562963301-1.394716750483.353263977530.0872225196167-0.08300142164710.4968217126360.6601586731740.0596370440839-0.12231107819-0.1202873902550.216007978924-0.1327719473440.4644845781940.115997165929-0.02742321630610.4174930571890.1542211341660.71048137914143.601963867914.015926461842.2960731579
32.34032939869-0.0668720573472-1.453755264462.20084787248-1.608989235394.327958416970.8212805561990.08154027226010.9274038655651.07868212342-0.833817673259-0.710274789105-1.20456763320.212554384132-0.05139333309160.976349855647-0.263339266198-0.1605489535470.7664706040650.3389335160911.0014462049219.675572910210.694191749858.3259656925
44.043867250831.50556221179-1.807051250762.565314129490.2867391573012.54301910117-0.6002578469051.86069570286-0.000197256057468-0.4925904854770.554598243827-0.1491843025830.659643204238-0.944260930161-0.05691685990880.3825421314250.197465080997-0.01753771290610.5527683000390.4349837541320.48387919809731.01267257692.8466320727238.8262549766
52.825170634380.130935707225-0.4967983501532.5897748397-0.6344198607263.00923055938-0.1131148677181.790602206840.102464693384-0.9217178541760.3478082125730.484751875150.25543272988-0.880228083458-0.2490398572660.844908073728-0.216867574898-0.01861487733791.467206874130.1717839959660.656267449974-2.6530427726339.1860055772-15.8737741127
62.761208765181.690233460251.466938141073.47432871489-2.32246536265.05471785066-0.1766579438031.036167427631.11679355096-0.1126185290120.5709527396741.01871909309-1.10667393671-1.10562050476-0.2045806566890.6386051889830.15785688351-0.001773709010751.217386666940.4912850533551.03098537776-25.575571618338.02875281215.48137046305
73.92832859410.672617264698-0.6500647584772.00193592017-1.661849709952.49523671024-0.505851613660.637371692973-0.565177165019-0.4279990299460.5106948941470.1608793998930.297733281284-0.4131525192810.05246738681730.468890748715-0.06068298391740.04801158633470.374237592937-0.03935001709620.549525587966-14.297192398321.021533854510.1417479822
83.38620843851-0.0136343981645-1.291983259062.96034096846-1.50295285156.34748391214-0.2720547955940.3618101815550.287031634652-0.03313234304690.198162572932-0.126924234052-0.350878931834-0.3546808377360.1128877911720.188197758476-0.0232209022635-0.09055714703290.437248083427-0.02846223961620.4442322907882.9661121377837.433163937311.7896759027
94.244698551550.888217656255-1.729997305772.89796964507-1.082666048143.24002737506-0.2247813864860.780824125148-0.376723633458-0.4529287648640.414284042404-0.007731654864260.427721523857-0.474854705777-0.1789219331290.420576320519-0.07969370020420.01801015154180.359343524977-0.07043124283770.461703745349-10.194331646220.87752006048.85976629614
103.49082773155-2.27223980476-0.09850225916562.48526091544-0.583803657745.16826757331-0.0431365786078-0.591733990942-0.04792250366511.464238522080.0184532047759-0.154681005751-0.483599181279-0.6924767343930.03644513681140.869428407554-0.21175455093-0.0575144380320.7456311514560.1021374515270.472115868174-10.7504261487-6.6364949280183.9942255353
117.82856374377-1.32750028918-5.766447680629.523167546081.286166736267.780047534260.890813654982-0.263763634241.009988193310.676433477631-0.09842993557131.0879979146-2.05717715392-1.64239197832-0.785358790281.288960676470.2312670849840.1273214074621.120039909660.1719535680040.887262781879-16.50219110561.273017274382.1755583688
122.36237757633-1.819248185890.4805032556861.4006582139-0.3624487655070.2472108838290.9452767395240.4665139259971.336197017430.5902569353691.245201329240.703836422559-1.19909891787-2.63319237103-1.721377649291.199824576710.5607171912360.02247825790331.69110481650.6097469789071.79686835874-20.88460544885.0185032184772.8850650807
134.201458830974.00250288301-5.726379963969.51292687256-4.807172929237.870351590130.3347106245462.058993367730.3498243180641.037440336150.00270565030641.09675155751-0.667670669606-1.48623208955-0.370682822980.894926600393-0.0530641266424-0.06579529782250.9524598605720.1140894600860.637190004322-16.6524721299-6.6058413410977.6478035319
144.2896439731-3.46424714672-2.114570514977.22138331748-0.9398978421912.69038274339-0.05934932356410.7253213504070.00482020853326-0.363327913035-0.05166783821590.37511084986-0.178781458936-0.6236309632470.1031188123540.758567522156-0.31183444463-0.2059534048020.8554752660460.1612806264970.413890229528-5.54014572152-9.6449701595370.5636086538
153.15582932343-2.27305274023-1.310212364794.65896458719-0.1752313630473.46914598223-0.08455560737280.636562798951-0.211722958746-0.199852993895-0.1318569870880.2437660444490.108064613923-0.5900313135260.2174052992930.588889328615-0.313664314614-0.1579230573020.7059989460090.06313331580740.450221655929-4.32649034191-16.869211745870.9987801877
162.964618103281.27492212706-4.597278862170.941641683867-2.636159975768.170093594461.166917130950.2802764273341.30157222533-0.279707242919-0.372582762463-1.25476017824-1.35791283949-0.452782956122-0.70217725170.9765517597550.1564016978090.3149154679870.8862490428590.3439168100571.6760954229132.561518490860.8306697473-11.784439046
175.319935085336.084623428156.207902493399.892618795299.628923165389.41875429628-0.0253819117516-0.748941877668-2.067829586120.706760784670.0207561621948-0.8998001944090.09791613437121.44840753711-0.1316300485140.906405912542-0.2225362126270.1238779220191.386684013990.6050114745991.4999278247141.771184492160.9507173838-14.2774249135
188.976506399326.45413162407-6.775651519364.77922073951-4.941784563435.130473515921.02872738716-2.52262526192-1.588464947820.62578947323-0.998710871036-1.8598240344-0.6550377221972.33474005167-0.0006559284154830.894222829604-0.04686085498510.002062022799961.255252207760.2401466294551.1963564453936.681686083753.50099880420.213292598977
194.420093769241.42866545694-3.269180008252.019893124830.9804655891955.06654874664-0.01487480160030.00532113585520.3710935800390.0174654904147-0.0298098662633-2.29074422594-0.4114696202963.13281712377-0.2101836766240.845215502475-0.00815111904588-0.1730299843061.61729346820.4466107153612.1815315283348.124941464252.1455718648-4.54778720283
205.926534887540.716234210122-0.8750257607881.416796039131.227453776428.357782090740.2630869710190.30733569696-0.254726840667-0.0115048288705-0.11900354373-1.764677259341.03180796043-0.1774049202240.1199923115440.7565998128380.1774223482670.3066288550410.617625543950.3239329183891.3350498643428.432970582345.2197442426-13.0408295087
214.38465097270.132059238578-2.163805324362.555516434160.784422124997.650782249190.3345210818960.667651315140.704709988876-0.253091000264-0.344171830586-1.177230831570.139343439217-0.351350949650.04325236432130.7204359919170.04873270008350.2897359549850.6566245234760.3493595341191.0101479187924.366254231749.7094778168-13.4285585421
229.6024546024.497271143173.697632138445.107847366360.4041956635632.38950206727-1.192304633122.15547518852-0.234567115914-1.491914234140.511808023927-0.6721704625060.4920727084021.443978021230.5086963064521.207811361830.02343663167090.5586514732981.442747625330.1954262071050.97234651887524.2227113643.3446142649-28.9818283846
234.149436806320.40974416753-2.086379145769.10412852825.353209140496.98048750498-0.2921117573021.237706932370.144626128553-1.6629652214-0.3753094178340.848934432001-0.619081164869-2.123181669670.5928020252970.651168830654-0.00367624884420.0370821615691.297439727890.2564243171390.72012887219112.967902048246.6053759912-19.4017594104
246.43848220916-0.5702386132245.42749012060.420168111369-0.1917878189684.79938963457-0.872025308194-1.34620366930.876750019741-0.1614546674180.0858584924649-0.2482231637260.6851557503581.402611633780.7214715278160.6484631079280.1456570908760.1752672902180.8876280010960.2212596024721.2076607746739.178864313255.3634149678-8.14515537426
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 19 through 251 )AA19 - 2511 - 233
22chain 'A' and (resid 252 through 293 )AA252 - 293234 - 275
33chain 'A' and (resid 294 through 431 )AA294 - 431276 - 413
44chain 'A' and (resid 432 through 614 )AA432 - 614414 - 596
55chain 'B' and (resid 19 through 129 )BH19 - 1291 - 111
66chain 'B' and (resid 130 through 194 )BH130 - 194112 - 176
77chain 'B' and (resid 195 through 293 )BH195 - 293177 - 275
88chain 'B' and (resid 294 through 431 )BH294 - 431276 - 413
99chain 'B' and (resid 432 through 614 )BH432 - 614414 - 596
1010chain 'E' and (resid 334 through 353 )ET334 - 3531 - 20
1111chain 'E' and (resid 354 through 375 )ET354 - 37521 - 42
1212chain 'E' and (resid 376 through 390 )ET376 - 39043 - 57
1313chain 'E' and (resid 391 through 403 )ET391 - 40358 - 70
1414chain 'E' and (resid 404 through 459 )ET404 - 45971 - 126
1515chain 'E' and (resid 460 through 526 )ET460 - 526127 - 188
1616chain 'F' and (resid 333 through 353 )FV333 - 3531 - 21
1717chain 'F' and (resid 354 through 363 )FV354 - 36322 - 31
1818chain 'F' and (resid 364 through 380 )FV364 - 38032 - 48
1919chain 'F' and (resid 381 through 394 )FV381 - 39449 - 62
2020chain 'F' and (resid 395 through 421 )FV395 - 42163 - 89
2121chain 'F' and (resid 422 through 459 )FV422 - 45990 - 127
2222chain 'F' and (resid 460 through 479 )FV460 - 479128 - 147
2323chain 'F' and (resid 480 through 506 )FV480 - 506148 - 174
2424chain 'F' and (resid 507 through 528 )FV507 - 528175 - 190

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