+Open data
-Basic information
Entry | Database: PDB / ID: 7tal | ||||||
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Title | LOV2-DARPIN fusion : D4_deltaDARP | ||||||
Components | D4_deltaDARP LOV2-DARPin fusion | ||||||
Keywords | DE NOVO PROTEIN / light switching | ||||||
Function / homology | FLAVIN MONONUCLEOTIDE Function and homology information | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.1 Å | ||||||
Authors | Mittl, P. | ||||||
Funding support | 1items
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Citation | Journal: To Be Published Title: LOV2-DARPIN fusion : D4_deltaDARP Authors: Mittl, P.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7tal.cif.gz | 65 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7tal.ent.gz | 45 KB | Display | PDB format |
PDBx/mmJSON format | 7tal.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ta/7tal ftp://data.pdbj.org/pub/pdb/validation_reports/ta/7tal | HTTPS FTP |
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-Related structure data
Related structure data | 2v0wS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 22415.627 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli) |
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#2: Chemical | ChemComp-FMN / |
#3: Chemical | ChemComp-MG / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.98 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 1 M lithium sulphate, 2% PEG8000, pH undefined |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 6, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.1→43.88 Å / Num. obs: 46107 / % possible obs: 83.5 % / Redundancy: 12.4 % / CC1/2: 0.999 / Rmerge(I) obs: 0.044 / Rpim(I) all: 0.013 / Net I/σ(I): 20.9 |
Reflection shell | Resolution: 1.1→1.19 Å / Redundancy: 9.7 % / Mean I/σ(I) obs: 1.9 / Num. unique obs: 2305 / CC1/2: 0.702 / % possible all: 74.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2V0W Resolution: 1.1→43.88 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.963 / SU B: 1.834 / SU ML: 0.038 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.055 / ESU R Free: 0.057 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 81.41 Å2 / Biso mean: 18.628 Å2 / Biso min: 8.41 Å2
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Refinement step | Cycle: final / Resolution: 1.1→43.88 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.102→1.131 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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