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Yorodumi- PDB-7su1: Crystal structure of an acidic pH-selective Ipilimumab variant Ip... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7su1 | ||||||
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Title | Crystal structure of an acidic pH-selective Ipilimumab variant Ipi.106 in complex with CTLA-4 | ||||||
Components |
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Keywords | IMMUNE SYSTEM / Immunoglobulin / checkpoint / antibody / complex | ||||||
Function / homology | Function and homology information protein complex involved in cell adhesion / negative regulation of regulatory T cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CTLA4 inhibitory signaling / negative regulation of B cell proliferation / negative regulation of T cell proliferation / B cell receptor signaling pathway / T cell receptor signaling pathway / adaptive immune response ...protein complex involved in cell adhesion / negative regulation of regulatory T cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / clathrin-coated endocytic vesicle / CTLA4 inhibitory signaling / negative regulation of B cell proliferation / negative regulation of T cell proliferation / B cell receptor signaling pathway / T cell receptor signaling pathway / adaptive immune response / immune response / positive regulation of apoptotic process / external side of plasma membrane / DNA damage response / perinuclear region of cytoplasm / Golgi apparatus / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.53 Å | ||||||
Authors | Lee, P.S. / Chau, B. / Strop, P. | ||||||
Funding support | 1items
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Citation | Journal: Mabs / Year: 2022 Title: Improved therapeutic index of an acidic pH-selective antibody. Authors: Lee, P.S. / MacDonald, K.G. / Massi, E. / Chew, P.V. / Bee, C. / Perkins, P. / Chau, B. / Thudium, K. / Lohre, J. / Nandi, P. / Deyanova, E.G. / Barman, I. / Gudmundsson, O. / Dollinger, G. ...Authors: Lee, P.S. / MacDonald, K.G. / Massi, E. / Chew, P.V. / Bee, C. / Perkins, P. / Chau, B. / Thudium, K. / Lohre, J. / Nandi, P. / Deyanova, E.G. / Barman, I. / Gudmundsson, O. / Dollinger, G. / Sproul, T. / Engelhardt, J.J. / Strop, P. / Rajpal, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7su1.cif.gz | 267.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7su1.ent.gz | 180.8 KB | Display | PDB format |
PDBx/mmJSON format | 7su1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/su/7su1 ftp://data.pdbj.org/pub/pdb/validation_reports/su/7su1 | HTTPS FTP |
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-Related structure data
Related structure data | 7su0C 3oskS 4nm4S 5truS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 24786.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) |
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#2: Antibody | Mass: 23527.035 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human) |
#3: Protein | Mass: 13555.421 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CTLA4, CD152 / Production host: Homo sapiens (human) / References: UniProt: P16410 |
#4: Sugar | ChemComp-NAG / |
#5: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.62 Å3/Da / Density % sol: 65.98 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M sodium citrate tribasic dehydrate, 22% PEG 3,350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER2 X 9M / Detector: PIXEL / Date: Oct 28, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.526→50 Å / Num. obs: 28083 / % possible obs: 94.6 % / Redundancy: 13.3 % / Biso Wilson estimate: 65.73 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.101 / Rpim(I) all: 0.029 / Net I/σ(I): 15.9 |
Reflection shell | Resolution: 2.526→2.652 Å / Mean I/σ(I) obs: 1.5 / Num. unique obs: 1404 / CC1/2: 0.64 / Rpim(I) all: 0.547 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5TRU, 4NM4, 3OSK Resolution: 2.53→37.52 Å / SU ML: 0.3863 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 29.349 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 75.45 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.53→37.52 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -17.3064912051 Å / Origin y: -55.6365047367 Å / Origin z: 18.4165802296 Å
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Refinement TLS group | Selection details: all |