Details | Type | Solution-ID | Contents | Label | Solvent system |
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solution1 | 300 uM U-1H RNA (38-MER), 300 uM U-1H FMN, 100% D2O | Tx041-FMN | 100% D2Osolution2 | 300 uM A-H, C-D, G-H, U-D RNA (38-MER), 300 uM U-1H FMN, 100% D2O | AG-Tx041-FMN | 100% D2Osolution3 | 300 uM A-H, C-H, G-D, U-D RNA (38-MER), 300 uM U-1H FMN, 100% D2O | AC-Tx041-FMN | 100% D2Osolution4 | 300 uM A-D, C-D, G-H, U-H RNA (38-MER), 300 uM U-H FMN, 100% D2O | GU-Tx041-FMN | 100% D2Osolution5 | 300 uM A-8D, C-H, G-H, U-5,6D2 RNA (38-MER), 300 uM U-1H FMN, 100% D2O | A2rUrGC-Tx041-FMN | 100% D2Osolution6 | 300 uM [U-100% 13C; U-100% 15N] RNA (38-MER), 300 uM U-1H FMN, 90% H2O/10% D2O | 13C/15N-Tx041-FMN | 90% H2O/10% D2Osolution7 | 300 uM [U-100% 13C; U-100% 15N] RNA (38-MER), 300 uM dioxopyrimidine-13C4, 15N2 FMN, 90% H2O/10% D2O | 13C/15N-Tx041-13C/15N-FMN | 90% H2O/10% D2O | | | | | | | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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300 uMRNA (38-MER) | U-1H | 1 | 300 uMFMNU-1H | 1 | 300 uMRNA (38-MER) | A-H, C-D, G-H, U-D | 2 | 300 uMFMNU-1H | 2 | 300 uMRNA (38-MER) | A-H, C-H, G-D, U-D | 3 | 300 uMFMNU-1H | 3 | 300 uMRNA (38-MER) | A-D, C-D, G-H, U-H | 4 | 300 uMFMNU-H | 4 | 300 uMRNA (38-MER) | A-8D, C-H, G-H, U-5,6D2 | 5 | 300 uMFMNU-1H | 5 | 300 uMRNA (38-MER) | [U-100% 13C; U-100% 15N] | 6 | 300 uMFMNU-1H | 6 | 300 uMRNA (38-MER) | [U-100% 13C; U-100% 15N] | 7 | 300 uMFMNdioxopyrimidine-13C4, 15N2 | 7 | | | | | | | | | | | | | | | | | | | | | |
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Sample conditions | Conditions-ID | Ionic strength | Label | pH | Pressure (kPa) | Temperature (K) |
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1 | 20 mM MgCl2 mM308K7.5 1 atm308 K2 | 20 mM MgCl2 mM283K6.5 1 atm283 K | | | | | | | | | |
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