Isoform4ofPDZandLIMdomainprotein7,Sortingnexin-17fusion / LIM mineralization protein / LMP / Protein enigma
Mass: 11317.567 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PDLIM7, ENIGMA, SNX17, KIAA0064 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9NR12, UniProt: Q15036
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
1
isotropic
1
2D 1H-13C HSQC
1
3
1
isotropic
1
3D HN(CA)CB
1
4
1
isotropic
1
3DCBCA(CO)NH
1
5
1
isotropic
1
3D HNCO
1
6
1
isotropic
1
3DHBHA(CO)NH
1
7
1
isotropic
1
3DH(CCO)NH
1
8
1
isotropic
1
3DH(CCO)NH
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Sample preparation
Details
Type: solution Contents: 1 mM [U-13C; U-15N] PDLIM7_SNX17_Fusion, 95% H2O/5% D2O Details: Protein was gel filtered in a buffer containing 75 mM NaCl, 20 mM Bis-Tris (pH 5.8), 4 mM DTT. Label: PDLIM7_SNX17_Fusion / Solvent system: 95% H2O/5% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
1mM
PDLIM7_SNX17_Fusion
[U-13C; U-15N]
1
75mM
NaCl
naturalabundance
1
20mM
Bis-Tris
naturalabundance
1
4mM
DTT
naturalabundance
1
Sample conditions
Details: Protein was gel filtered in a buffer containing 75 mM NaCl, 20 mM Bis-Tris (pH 5.8), 4 mM DTT. Ionic strength: 75 mM NaCl mM / Label: Condition 1 / pH: 5.8 / Pressure: 1 atm / Temperature: 298 K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz
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