+Open data
-Basic information
Entry | Database: PDB / ID: 7qap | ||||||
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Title | Three-dimensional structure of the PGAM5 G17L mutant TMD | ||||||
Components | Serine/threonine-protein phosphatase PGAM5, mitochondrial | ||||||
Keywords | MEMBRANE PROTEIN / PARL / MITOCHONDRIA | ||||||
Function / homology | Function and homology information negative regulation of cold-induced thermogenesis / myosin phosphatase activity / positive regulation of mitochondrial fission / Receptor Mediated Mitophagy / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / phosphatase activity / necroptotic process / GTPase activator activity / macroautophagy ...negative regulation of cold-induced thermogenesis / myosin phosphatase activity / positive regulation of mitochondrial fission / Receptor Mediated Mitophagy / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / phosphatase activity / necroptotic process / GTPase activator activity / macroautophagy / mitochondrial outer membrane / mitochondrial inner membrane / protein-containing complex binding / mitochondrion Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Silber, M. / Muhle-Goll, C. | ||||||
Funding support | Germany, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2022 Title: Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state. Authors: Siebert, V. / Silber, M. / Heuten, E. / Muhle-Goll, C. / Lemberg, M.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7qap.cif.gz | 215.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7qap.ent.gz | 184.8 KB | Display | PDB format |
PDBx/mmJSON format | 7qap.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qa/7qap ftp://data.pdbj.org/pub/pdb/validation_reports/qa/7qap | HTTPS FTP |
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-Related structure data
Related structure data | 7qalC 7qamC 7qaoC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3360.907 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) References: UniProt: Q96HS1, protein-serine/threonine phosphatase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 0.5 mM G17L, trifluoroethanol/water / Label: G17L / Solvent system: trifluoroethanol/water |
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Sample | Conc.: 0.5 mM / Component: G17L / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength: 1 Not defined / Label: G17L / pH: 5.0 / Pressure: 1 atm / Temperature: 300 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: closest to the average | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |