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- PDB-7q42: Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2... -

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Basic information

Entry
Database: PDB / ID: 7q42
TitleCrystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of chromatin reader BAZ2B
Components
  • Bromodomain adjacent to zinc finger domain protein 2B
  • E3 ubiquitin-protein ligase HERC2
KeywordsPROTEIN BINDING / 7-bladed beta-propeller E3 ubiquitin-protein ligase HERC2 RCC1-like Domain2 RLD2 BAZ2B chromatin reader DXDKDED motif
Function / homology
Function and homology information


SUMO binding / HECT-type E3 ubiquitin transferase / SUMOylation of DNA damage response and repair proteins / centriole / guanyl-nucleotide exchange factor activity / Nonhomologous End-Joining (NHEJ) / intracellular protein transport / G2/M DNA damage checkpoint / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation ...SUMO binding / HECT-type E3 ubiquitin transferase / SUMOylation of DNA damage response and repair proteins / centriole / guanyl-nucleotide exchange factor activity / Nonhomologous End-Joining (NHEJ) / intracellular protein transport / G2/M DNA damage checkpoint / ubiquitin protein ligase activity / Antigen processing: Ubiquitination & Proteasome degradation / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Processing of DNA double-strand break ends / spermatogenesis / proteasome-mediated ubiquitin-dependent protein catabolic process / protein ubiquitination / chromatin remodeling / DNA repair / DNA damage response / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / membrane / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
HERC2, APC10 domain / HERC2, zinc finger, ZZ-type / Beta-propeller repeat TECPR / Mib-herc2 / Mib/herc2 domain superfamily / Mib_herc2 / MIB/HERC2 domain profile. / Beta propeller repeats in Physarum polycephalum tectonins, Limulus lectin L-6 and animal hypothetical proteins. / CPH domain / Mouse development and cellular proliferation protein Cullin-7 ...HERC2, APC10 domain / HERC2, zinc finger, ZZ-type / Beta-propeller repeat TECPR / Mib-herc2 / Mib/herc2 domain superfamily / Mib_herc2 / MIB/HERC2 domain profile. / Beta propeller repeats in Physarum polycephalum tectonins, Limulus lectin L-6 and animal hypothetical proteins. / CPH domain / Mouse development and cellular proliferation protein Cullin-7 / Regulator of chromosome condensation (RCC1) signature 2. / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / APC10/DOC domain / Anaphase-promoting complex, subunit 10 (APC10) / DOC domain profile. / Anaphase-promoting complex, subunit 10 (APC10) / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Cytochrome b5 family, heme-binding domain profile. / Cytochrome b5-like heme/steroid binding domain / Cytochrome b5-like heme/steroid binding domain superfamily / Cytochrome b5-like Heme/Steroid binding domain / Cytochrome b5-like Heme/Steroid binding domain / HECT domain / HECT, E3 ligase catalytic domain / HECT-domain (ubiquitin-transferase) / HECT domain profile. / Domain Homologous to E6-AP Carboxyl Terminus with / BAZ2A/BAZ2B, bromodomain / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / DDT domain / DDT domain / WHIM2 domain / Williams-Beuren syndrome DDT (WSD), D-TOX E motif / DDT domain profile. / domain in different transcription and chromosome remodeling factors / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / Methyl-CpG binding domain / Methyl-CpG DNA binding / Methyl-CpG binding domain / Methyl-CpG-binding domain (MBD) profile. / DNA-binding domain superfamily / PHD-finger / Zinc finger PHD-type profile. / Zinc finger, PHD-finger / Galactose-binding-like domain superfamily / Zinc finger, PHD-type / PHD zinc finger / Zinc finger, FYVE/PHD-type / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / Ribosomal protein L2, domain 2 / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
CITRIC ACID / E3 ubiquitin-protein ligase HERC2 / Bromodomain adjacent to zinc finger domain protein 2B
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95002486823 Å
AuthorsDemenge, A. / Howard, E. / Cousido-Siah, A. / Mitschler, A. / Podjarny, A. / McEwen, A.G. / Trave, G.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of chromatin reader BAZ2B
Authors: Demenge, A. / Howard, E. / Cousido-Siah, A. / Mitschler, A. / Podjarny, A. / McEwen, A.G. / Trave, G.
History
DepositionOct 29, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 16, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: E3 ubiquitin-protein ligase HERC2
C: E3 ubiquitin-protein ligase HERC2
E: E3 ubiquitin-protein ligase HERC2
D: Bromodomain adjacent to zinc finger domain protein 2B
B: Bromodomain adjacent to zinc finger domain protein 2B
F: Bromodomain adjacent to zinc finger domain protein 2B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)134,0779
Polymers133,5006
Non-polymers5763
Water15,331851
1
A: E3 ubiquitin-protein ligase HERC2
B: Bromodomain adjacent to zinc finger domain protein 2B


Theoretical massNumber of molelcules
Total (without water)44,5002
Polymers44,5002
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: E3 ubiquitin-protein ligase HERC2
D: Bromodomain adjacent to zinc finger domain protein 2B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)44,6923
Polymers44,5002
Non-polymers1921
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
E: E3 ubiquitin-protein ligase HERC2
F: Bromodomain adjacent to zinc finger domain protein 2B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)44,8844
Polymers44,5002
Non-polymers3842
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)107.980, 107.980, 241.560
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number96
Space group name H-MP43212
Space group name HallP4nw2abw
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z+3/4
#3: y+1/2,-x+1/2,z+1/4
#4: x+1/2,-y+1/2,-z+1/4
#5: -x+1/2,y+1/2,-z+3/4
#6: -x,-y,z+1/2
#7: y,x,-z
#8: -y,-x,-z+1/2

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Components

#1: Protein E3 ubiquitin-protein ligase HERC2 / HECT domain and RCC1-like domain-containing protein 2 / HECT-type E3 ubiquitin transferase HERC2


Mass: 42770.566 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HERC2 / Production host: Escherichia coli (E. coli)
References: UniProt: O95714, HECT-type E3 ubiquitin transferase
#2: Protein/peptide Bromodomain adjacent to zinc finger domain protein 2B / hWALp4


Mass: 1729.513 Da / Num. of mol.: 3 / Source method: obtained synthetically
Details: n-terminally biotinylated peptide via 1,13-diamino-4,7,10-trioxatridecan-succinamic acid
Source: (synth.) Homo sapiens (human) / References: UniProt: Q9UIF8
#3: Chemical ChemComp-CIT / CITRIC ACID / Citric acid


Mass: 192.124 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C6H8O7
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 851 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.93 Å3/Da / Density % sol: 57.99 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 8
Details: hanging drop 20% PEG 3350 0.18M tris amonium citrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.978564 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 9, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.978564 Å / Relative weight: 1
ReflectionResolution: 1.95→49.29 Å / Num. obs: 104649 / % possible obs: 99.92 % / Redundancy: 27.0621601735 % / Biso Wilson estimate: 29.4271306625 Å2 / CC1/2: 0.998 / Net I/σ(I): 10.93
Reflection shellResolution: 1.95→2 Å / Mean I/σ(I) obs: 1.03 / Num. unique obs: 7616 / CC1/2: 0.61

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Processing

Software
NameVersionClassification
PHENIX1.12_2829refinement
PHENIX1.12_2829refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3KCI

3kci


Resolution: 1.95002486823→49.289620536 Å / SU ML: 0.197426353888 / Cross valid method: FREE R-VALUE / σ(F): 1.3364811143 / Phase error: 19.8875984756
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.206296338153 5167 4.94345687989 %
Rwork0.167870694125 99355 -
obs0.16974605582 104522 99.9168331597 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 33.9076128539 Å2
Refinement stepCycle: LAST / Resolution: 1.95002486823→49.289620536 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8433 0 39 851 9323
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01067798198878638
X-RAY DIFFRACTIONf_angle_d1.1341394845911665
X-RAY DIFFRACTIONf_chiral_restr0.08300374245781293
X-RAY DIFFRACTIONf_plane_restr0.00674149692571513
X-RAY DIFFRACTIONf_dihedral_angle_d20.0233136723092
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.95002486823-1.97220.3145612906381770.2775805060563251X-RAY DIFFRACTION99.0179087233
1.9722-1.99540.3308304104951780.2588239588493254X-RAY DIFFRACTION99.4494349464
1.9954-2.01970.2966771876241520.234906606483247X-RAY DIFFRACTION99.8237885463
2.0197-2.04530.2604915131041430.2242323731583318X-RAY DIFFRACTION99.9711149624
2.0453-2.07220.238884396621770.2085034535613238X-RAY DIFFRACTION99.941469125
2.0722-2.10060.2488663900091670.2060420822673281X-RAY DIFFRACTION99.9710060887
2.1006-2.13060.2243947322031720.2072661624113255X-RAY DIFFRACTION99.9416739574
2.1306-2.16240.2434694383841730.2023852683463274X-RAY DIFFRACTION99.9420121774
2.1624-2.19620.2543064295391690.1996506408883248X-RAY DIFFRACTION99.9707431246
2.1962-2.23220.2190396410391630.1923962275873300X-RAY DIFFRACTION99.9711316397
2.2322-2.27070.2358080685421580.1905148153013251X-RAY DIFFRACTION99.8828010548
2.2707-2.3120.2362657166341530.1891705234783315X-RAY DIFFRACTION100
2.312-2.35640.2165795173932120.1797803290283247X-RAY DIFFRACTION100
2.3564-2.40450.2226626281171600.1800889737963338X-RAY DIFFRACTION100
2.4045-2.45680.2103926836141660.1765466823743263X-RAY DIFFRACTION100
2.4568-2.5140.2253442991531740.1738414238463282X-RAY DIFFRACTION99.9710731848
2.514-2.57680.2087969087351910.1709190718033259X-RAY DIFFRACTION100
2.5768-2.64650.2216726839041790.1759206967623313X-RAY DIFFRACTION99.9713713141
2.6465-2.72440.2325860825371760.1740178268543279X-RAY DIFFRACTION100
2.7244-2.81230.2227262939481840.1750087159833277X-RAY DIFFRACTION100
2.8123-2.91280.2256351730151710.1722151186813328X-RAY DIFFRACTION100
2.9128-3.02940.2098278372391750.1710619770533297X-RAY DIFFRACTION100
3.0294-3.16730.1859831631211490.1650752404493362X-RAY DIFFRACTION100
3.1673-3.33420.181449369921800.1651595679633334X-RAY DIFFRACTION99.9715504979
3.3342-3.54310.2017844245081720.1589431981953342X-RAY DIFFRACTION100
3.5431-3.81650.1725162563581780.141476446823351X-RAY DIFFRACTION100
3.8165-4.20040.1523476394761710.1255823223153370X-RAY DIFFRACTION100
4.2004-4.80780.1644458679371680.1313702875963426X-RAY DIFFRACTION100
4.8078-6.05560.1827244214791730.1528218753753435X-RAY DIFFRACTION100
6.0556-49.2896205360.2361961972132060.1836529028483620X-RAY DIFFRACTION99.7133176961
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.459876582271-0.152557985407-0.0207058417710.2147823837610.1578792737940.352638901550.1247482027010.214048708857-0.0490932971022-0.147252496593-0.2505356439570.03749019256670.0373270829671-0.1616626772145.00329831556E-80.2297674695980.08364058718610.007570369107540.285455833446-0.008031264424590.133393052541-21.2510955594-40.5841427688-49.804185506
20.444975869155-0.45390185925-0.002423559510120.7045538233910.2438768207930.5372570023850.02153581241570.0726633042849-0.0389597243465-0.0452289267268-0.1070809180580.0960380260266-0.0755044996593-0.1553735263015.95517702452E-90.132567634320.0578343747817-0.002636243594690.2301533845020.01014457609640.139174733318-29.3094701278-30.4246466223-38.5030860433
30.382916341482-0.3115615204040.1084715678690.3685970629420.09912147147420.582517143023-0.0118498931061-0.02589280718710.0907007933724-0.0641853407111-0.05131596672770.0714897511073-0.164910574413-0.2046933552627.60054279352E-80.1900013232690.09654233498990.01529054880680.239182104470.00602820603190.205352032107-29.1898812106-22.022506124-31.0460737249
40.787502784822-0.259764134521-0.2123679764120.1703061260230.3499661833680.38801853772-0.0940559583344-0.07258748326690.07613131413750.02824508908180.040647828427-0.0401758765368-0.107243117137-0.08141779131741.49023555661E-80.2378571375420.05788191032490.009885283063550.1695552985490.005984948575730.166985837891-17.4801596943-23.8688647712-23.7149603345
51.01715877454-0.8788546932850.1092479136390.1885907855-0.02757055133440.614935141671-0.0236582765856-0.03825283451590.09168015730430.03369263602420.0212290052949-0.0774906844492-0.0174620372140.120102839225-5.83655001976E-100.1574368599760.008373370289810.006534036950780.1770011572840.02037994666660.146204662753-4.23919775846-32.7578101541-27.5434923637
60.1131059276380.1292975782990.1125738550760.3338092702870.1948818274780.3002822164620.0003336264454930.1656455831750.0525950131410.0323538728772-0.0452223129784-0.08368081821040.07490609507420.1577816666556.59639352919E-80.1537379524040.0542707160320.04650905446180.2689229629590.05771830658840.184108080644-3.7845134721-36.4629083551-42.9118098934
70.3600297067370.542954131876-0.1999580097440.3197432277560.1534372153260.4973074734440.05709641529990.0855794287410.0128973077821-0.0758904699082-0.000252511985289-0.02093215420150.03688699528640.02416335150439.65448306912E-80.217291434220.06686974194590.0370025086950.2388977336430.02669286877830.165393185685-10.3915761731-42.5640133137-45.9151593772
80.4859753572070.450848089480.02343748545490.213675669873-0.2187839760590.20632325209-0.1457754109770.3034016566310.00540692331422-0.1182774306170.04634775046630.02637941799390.235048144677-0.107595968502-9.66368763386E-90.213934899236-0.0189844576669-0.000957209461270.224231613744-0.002951828573820.1495136504-16.6911902314-59.8789631868-10.6551912743
90.3434729150370.204347241221-0.4803374119030.279937561722-0.03911907811410.0735791164277-0.07322037419270.216478378465-0.0976881576325-0.07232625333210.03293315217140.06914496046510.150188933001-0.1391566018399.78343814154E-80.205203712759-0.0444560610256-0.02166215396520.2413098407-0.03716705361490.158507699567-25.8777169408-66.3440641609-4.76025306556
100.5985038776940.502215116786-0.5402149613380.262363922858-0.07711961627870.989723839369-0.01874623557310.1114790321250.0674761600120.00477688566792-0.01790335469690.1069471097440.108581849904-0.1341508829671.23256591485E-80.130663517922-0.035775233687-0.004640980139760.177107084704-0.01917145522580.234992857391-33.3970601146-61.87247847386.17379262525
110.2870089183420.6614744498570.2425898198370.639123087461-0.1465171546480.3202853069950.0851571377579-0.03932745389220.03301703912620.0550054353053-0.1242700490830.1421224373380.0272880502561-0.06026413855449.72836836706E-90.188154198288-0.01610011254170.0391600670290.178109044361-0.02765510769590.200117364465-27.5590467311-54.623077527817.9728886847
120.7933908112160.4580819047360.1183640125380.5138150197960.2032939632120.4991262793840.0955532274841-0.04802012465960.08372661279560.0895743107646-0.0587128338177-0.0150808526562-0.04918170485880.05150983797451.93124490215E-80.193746975343-0.01584854398390.001729167751330.149485000997-0.009370154630510.141455299042-13.0276302664-47.529524647315.1256758357
130.562659285334-0.135885863098-0.5100129640020.2051048792570.08105829878430.6618438510240.07361902867750.04996903182190.04628906996530.0771898961388-0.0734100254431-0.0941510783635-0.1043256326640.0782532926459-4.47056298422E-80.172901617949-0.00424196068849-0.01173696454280.1711754899450.02374080561680.16092253899-7.30109419465-49.0580106827-1.01615471615
140.0853478821380.178247266466-0.1037190849950.0768310449271-0.1256303664590.2338446999140.05943259914640.138640802142-0.007719560276480.02013016205170.03882124331940.082312799813-0.0173147021258-0.08727051546741.46269404325E-70.1831604484090.0276357155624-0.00397977112530.2039581108670.02633201206410.15108850012-15.0433523957-50.5458026869-8.44170748546
150.953661546179-0.259495301289-0.034346909660.2620276923840.03117729588140.5519542955830.1054484215610.3706972097120.230757056583-0.0585787362512-0.096505451217-0.195002082902-0.0194723389190.01212130390842.06088100916E-80.1562799211390.008644086981590.03427376277070.2197397661660.05514606340930.25350442755126.4004098743-49.0835432503-30.248142359
160.602730347034-0.01406169451020.3720297301820.270408654927-0.5221461710590.6179722954730.04861601925380.121744267470.05374153925730.00171603796263-0.0973158782794-0.1721306614160.03697860974910.0423284120667-2.70379496929E-90.1362215810690.00925345461223-0.006405907025810.173224681949-0.01030264256040.33363016818936.3241806484-57.3055252288-19.7009708722
171.187489547810.1217547599360.2796868290620.286145336743-0.6017820088590.8932064347860.0424532750542-0.113288582769-0.0275329889650.0206153660842-0.00498333515977-0.3135564399960.01843417594620.0324636122659-1.98376545147E-80.15564396840.00800175452523-0.07839092727970.153228636910.01309313259770.3491665934335.5596771182-60.876065493-9.48886812786
181.60713224754-0.1707106269310.1831423901030.275677008567-0.3154913181670.5558959351960.0589165237638-0.216034107894-0.1223277415390.02067344205230.00903664949493-0.08158420337960.0423970395516-0.1258470925892.49571849625E-90.150913847844-0.0245603152869-0.0516500718090.1491977521050.03729261020.1495630958416.0934519286-62.5425208828-7.69941507807
190.413439251258-0.1944271544520.3693806599440.455927513114-0.1483457654120.2397622754550.09653947708340.0601292305973-0.00936168322830.0438956876401-0.0599388062520.00700700882045-0.0352849279191-0.15690715041-1.26502083502E-70.1403836037660.00341026432494-0.01181799373060.2172965745180.00223978578710.1687146777798.12654961421-55.1246812466-22.8353853139
200.1354752516850.01120645313130.1899191164470.154690498366-0.120790581480.03479435324540.05820828053040.151124828536-0.05331849939910.00318721554127-0.0044795064402-0.0915233832307-0.0080514994614-0.0548789849627.97651284526E-70.1871187099650.00111490269973-0.007141680596520.250049308613-0.0278280425080.19706837721914.6543113907-56.6014569674-30.984832185
21-0.0079847415655-0.0194762347555-0.005474879759040.0223898179769-0.03612557279770.0118068975441-0.253395041094-0.630321032657-0.130035588792-0.168350896272-0.0720861711496-0.2478464921340.765650989933-0.344143623146-2.04408512642E-70.4578763971980.0549490735429-0.006154671537050.3579441314130.005371309752870.509605655043-7.65215759767-68.41353630384.07521247908
220.0538918372847-0.0728025447881-0.0328304513780.05483848836440.01759577420230.0133915414873-0.27226128799-0.0668986182556-0.2147570548730.412799493509-0.212864112969-0.1667733481850.0115123474726-0.348487774261-4.30952807943E-60.428314914521-0.06548632208540.03459094171260.3965561408550.0366059703640.327714641274-20.3534610258-50.4334736581-32.1321607628
23-0.00046388735776-0.0226709821365-0.008972652131130.02134387607940.0252579490341-0.00105123805176-0.0919969420664-0.179410237030.230708414822-0.1016268843740.155056215810.0501543835722-0.4348490783440.04212225407021.11434026178E-50.550614277209-0.0010582239169-0.006999507376970.400578488549-0.1296400595190.56308945149718.002993711-40.9450814218-15.3024873788
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2958 through 3000 )
2X-RAY DIFFRACTION2chain 'A' and (resid 3001 through 3082 )
3X-RAY DIFFRACTION3chain 'A' and (resid 3083 through 3106 )
4X-RAY DIFFRACTION4chain 'A' and (resid 3107 through 3169 )
5X-RAY DIFFRACTION5chain 'A' and (resid 3170 through 3250 )
6X-RAY DIFFRACTION6chain 'A' and (resid 3251 through 3282 )
7X-RAY DIFFRACTION7chain 'A' and (resid 3283 through 3326 )
8X-RAY DIFFRACTION8chain 'C' and (resid 2959 through 3000 )
9X-RAY DIFFRACTION9chain 'C' and (resid 3001 through 3037 )
10X-RAY DIFFRACTION10chain 'C' and (resid 3038 through 3106 )
11X-RAY DIFFRACTION11chain 'C' and (resid 3107 through 3169 )
12X-RAY DIFFRACTION12chain 'C' and (resid 3170 through 3250 )
13X-RAY DIFFRACTION13chain 'C' and (resid 3251 through 3302 )
14X-RAY DIFFRACTION14chain 'C' and (resid 3303 through 3326 )
15X-RAY DIFFRACTION15chain 'E' and (resid 2959 through 3037 )
16X-RAY DIFFRACTION16chain 'E' and (resid 3038 through 3082 )
17X-RAY DIFFRACTION17chain 'E' and (resid 3083 through 3144 )
18X-RAY DIFFRACTION18chain 'E' and (resid 3145 through 3250 )
19X-RAY DIFFRACTION19chain 'E' and (resid 3251 through 3302 )
20X-RAY DIFFRACTION20chain 'E' and (resid 3303 through 3326 )
21X-RAY DIFFRACTION21chain 'D' and (resid 649 through 658 )
22X-RAY DIFFRACTION22chain 'B' and (resid 649 through 658 )
23X-RAY DIFFRACTION23chain 'F' and (resid 650 through 656 )

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