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- PDB-7ps1: Crystal structure of the receptor binding domain of SARS-CoV-2 be... -

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Basic information

Entry
Database: PDB / ID: 7ps1
TitleCrystal structure of the receptor binding domain of SARS-CoV-2 beta variant spike glycoprotein in complex with Beta-27 Fab
Components
  • Beta-27 Fab heavy chain
  • Beta-27 Fab light chain
  • Spike protein S1
KeywordsVIRAL PROTEIN / SARS-CoV-2 alpha variant / beta variant / gamma variant / delta variant / B.1.1.7 / B.1.351 / P.1 / B.1.617.2 / antibody / receptor-binding-domain / spike / neutralisation / VIRAL PROTEIN/IMMUNE SYSTEM
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
IODIDE ION / DI(HYDROXYETHYL)ETHER / Spike glycoprotein
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å
AuthorsZhou, D. / Ren, J. / Stuart, D.I.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MR/ N00065X/1 United Kingdom
CAMS Innovation Fund for Medical Sciences (CIFMS)2018-I2M-2-002 United Kingdom
CitationJournal: Cell Host Microbe / Year: 2022
Title: The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants.
Authors: Chang Liu / Daming Zhou / Rungtiwa Nutalai / Helen M E Duyvesteyn / Aekkachai Tuekprakhon / Helen M Ginn / Wanwisa Dejnirattisai / Piyada Supasa / Alexander J Mentzer / Beibei Wang / James ...Authors: Chang Liu / Daming Zhou / Rungtiwa Nutalai / Helen M E Duyvesteyn / Aekkachai Tuekprakhon / Helen M Ginn / Wanwisa Dejnirattisai / Piyada Supasa / Alexander J Mentzer / Beibei Wang / James Brett Case / Yuguang Zhao / Donal T Skelly / Rita E Chen / Sile Ann Johnson / Thomas G Ritter / Chris Mason / Tariq Malik / Nigel Temperton / Neil G Paterson / Mark A Williams / David R Hall / Daniel K Clare / Andrew Howe / Philip J R Goulder / Elizabeth E Fry / Michael S Diamond / Juthathip Mongkolsapaya / Jingshan Ren / David I Stuart / Gavin R Screaton /
Abstract: Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the antigenic structure of S, causing escape ...Alpha-B.1.1.7, Beta-B.1.351, Gamma-P.1, and Delta-B.1.617.2 variants of SARS-CoV-2 express multiple mutations in the spike protein (S). These may alter the antigenic structure of S, causing escape from natural or vaccine-induced immunity. Beta is particularly difficult to neutralize using serum induced by early pandemic SARS-CoV-2 strains and is most antigenically separated from Delta. To understand this, we generated 674 mAbs from Beta-infected individuals and performed a detailed structure-function analysis of the 27 most potent mAbs: one binding the spike N-terminal domain (NTD), the rest the receptor-binding domain (RBD). Two of these RBD-binding mAbs recognize a neutralizing epitope conserved between SARS-CoV-1 and -2, while 18 target mutated residues in Beta: K417N, E484K, and N501Y. There is a major response to N501Y, including a public IgVH4-39 sequence, with E484K and K417N also targeted. Recognition of these key residues underscores why serum from Beta cases poorly neutralizes early pandemic and Delta viruses.
History
DepositionSep 22, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 15, 2021Provider: repository / Type: Initial release
Revision 1.1Dec 29, 2021Group: Database references / Source and taxonomy / Structure summary
Category: citation / citation_author ...citation / citation_author / entity / entity_src_gen / struct_ref / struct_ref_seq / struct_ref_seq_dif
Item: _citation.pdbx_database_id_PubMed / _citation.title ..._citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name / _entity.pdbx_description / _entity_src_gen.gene_src_common_name / _entity_src_gen.pdbx_gene_src_gene / _struct_ref.db_code / _struct_ref.pdbx_align_begin / _struct_ref.pdbx_db_accession / _struct_ref.pdbx_seq_one_letter_code / _struct_ref_seq.db_align_beg / _struct_ref_seq.db_align_end / _struct_ref_seq.pdbx_db_accession
Revision 1.2Jan 26, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.year
Revision 1.3Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
E: Spike protein S1
A: Beta-27 Fab heavy chain
B: Beta-27 Fab light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)71,76018
Polymers70,3093
Non-polymers1,45115
Water2,342130
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8690 Å2
ΔGint-53 kcal/mol
Surface area27240 Å2
MethodPISA
Unit cell
Length a, b, c (Å)193.396, 85.745, 57.447
Angle α, β, γ (deg.)90.000, 99.730, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z

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Components

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Antibody , 2 types, 2 molecules AB

#2: Antibody Beta-27 Fab heavy chain


Mass: 23532.479 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody Beta-27 Fab light chain


Mass: 23129.549 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Protein / Sugars , 2 types, 2 molecules E

#1: Protein Spike protein S1


Mass: 23646.551 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#4: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 5 types, 144 molecules

#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: Cl
#7: Chemical
ChemComp-IOD / IODIDE ION / Iodide


Mass: 126.904 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: I
#8: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H10O3
#9: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 130 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.47 Å3/Da / Density % sol: 64.53 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 0.2 M Potassium iodide, 0.1 M MES pH 6.5 and 25% (w/v) PEG 4000.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97626 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: May 17, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97626 Å / Relative weight: 1
ReflectionResolution: 2.4→95 Å / Num. obs: 35126 / % possible obs: 96.7 % / Redundancy: 16.7 % / Biso Wilson estimate: 48.65 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.181 / Rpim(I) all: 0.042 / Net I/σ(I): 8.6
Reflection shellResolution: 2.4→2.44 Å / Mean I/σ(I) obs: 0.4 / Num. unique obs: 1395 / CC1/2: 0.599

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Processing

Software
NameVersionClassification
GDA1.19_4092data collection
PHENIX1.19_4092refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7NXA

7nxa


Resolution: 2.4→56.62 Å / SU ML: 0.3164 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 22.8132
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2151 1642 4.77 %
Rwork0.1839 32810 -
obs0.1854 34452 94.86 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 58.05 Å2
Refinement stepCycle: LAST / Resolution: 2.4→56.62 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4757 0 60 130 4947
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00364928
X-RAY DIFFRACTIONf_angle_d0.6526690
X-RAY DIFFRACTIONf_chiral_restr0.0445740
X-RAY DIFFRACTIONf_plane_restr0.0051859
X-RAY DIFFRACTIONf_dihedral_angle_d11.74741762
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4-2.470.3322950.31671925X-RAY DIFFRACTION67.47
2.47-2.550.32691110.29622352X-RAY DIFFRACTION82.96
2.55-2.640.31371340.27332661X-RAY DIFFRACTION91.79
2.64-2.750.30641320.24512760X-RAY DIFFRACTION96.27
2.75-2.870.26811440.21352886X-RAY DIFFRACTION99.38
2.87-3.020.25861400.21122864X-RAY DIFFRACTION100
3.02-3.210.25911430.20812870X-RAY DIFFRACTION100
3.21-3.460.19991660.18972831X-RAY DIFFRACTION100
3.46-3.810.21191520.16972883X-RAY DIFFRACTION99.97
3.81-4.360.17681360.1532905X-RAY DIFFRACTION100
4.36-5.490.16581440.13882904X-RAY DIFFRACTION100
5.49-56.620.1991450.17622969X-RAY DIFFRACTION99.87
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.554391964520.1745600374830.1175116834684.211568331890.006421096303684.490603455310.09428008276160.4176642464870.493624599324-0.595793012848-0.175737333535-1.15416148963-0.7384661289930.5858538854860.04692185803610.536733240842-0.03822822920760.1598767942160.5666559341590.002987345239930.76251311213876.1601899321-30.267641097911.6436129604
22.72686730463-0.1940366678080.7180802919623.396948256850.05358970337025.259625648990.0109957863880.1172729450460.2101694607170.08906944416380.00605216074938-0.628787542657-0.4614949332120.432662816836-0.04289820627260.3447298299580.03027524167360.04309562434040.3445850515050.003823874385540.45096348103566.7794724331-33.325068475617.5239943818
32.887414859411.31998233525-0.7534860758131.202532782340.2875736690441.74232472699-0.1706434886070.0240813522237-0.739123180952-0.109313741670.0560013618144-0.1030038843620.375492998883-0.05440535721360.07369497306530.4590191046610.0313459736442-0.01228043555370.360684142771-0.06446113914550.49849459384655.5047372427-50.811362271218.6390580986
42.719092976360.07884160558781.197107634585.16377422084-0.4013481000865.285637909820.2043493636310.4897014569130.2108326778610.275138772435-0.199263282264-1.16152203811-0.03367207604340.7313056251780.01045270459940.446427106499-0.04958258734370.05992195532160.357226867210.01544713632930.56092717171269.8313577184-31.386928675814.5125092593
54.09742246365-1.6447841979-1.933330124744.106000160431.780652653526.45798005134-0.0628352914332-0.249910192533-0.3650438020160.659146365751-0.2145625625560.3026596242980.599927414172-0.6250410846460.4706102345840.2948516363230.05951182769850.02322443846320.3730858606440.07860127725820.32695103389936.1882741067-41.979278572641.1865156397
63.4195804492-0.517084547063-1.73551470892.181791384921.177660696054.11159505002-0.126256682378-0.185958918171-0.5083145580730.635218475607-0.2437976940110.01619330443550.560141821465-0.16050022150.1507320870280.3534316907950.08130995478630.02596154885720.3639283750190.03996311444170.33274317781743.2561166089-44.266003984833.3177496326
73.593379304380.949639178191-1.138839733520.3751440307560.4663725464894.099484522140.0100837366775-0.2317289467830.206969641654-0.03607049887350.09934491942010.0102989719485-0.2397459757590.167607965962-0.04528532486050.4155809678270.001707954016790.001607114060190.304177494758-0.01412089603310.32168082421644.8257832591-35.063291703931.1254779505
84.42831632113-1.25615826761-0.8000656765564.353830866870.9369203873914.61411114512-0.0361526395677-0.4871894281310.3616405757730.5961293026520.123174663564-0.407568188372-0.3226911022950.353995334903-0.07964909241260.4058060125760.0168848230365-0.0512750161530.525674418816-0.01293354219390.39490912328347.7194468085-34.990786634437.9143359896
94.67407948456-0.666912464971-0.9603594178720.7806905962641.499548348664.55636497766-0.300841985264-0.6411742471520.07252257889360.1603808162080.561586257975-0.517732035850.08265211388550.0534817512506-0.005447417049310.355467001820.0722771690678-0.002005811206880.424448059450.05838719000150.36525438772642.957044758-38.491497801341.1512211336
103.32087058655-1.74994871617-1.060789091631.244324269861.815152195624.61297281166-0.1235428356280.0164605389646-0.2024104457120.1069710055040.03723014408570.2565921247850.335017239458-0.238537378639-0.0768311368580.4664560041990.02535590712610.04003752439360.3955005711940.02075265802310.42231496243838.4593049222-41.741769722428.5541124562
113.83338732621.05414407776-0.1756205095971.135903005291.564083488333.22087290494-0.331248816749-0.7787072806790.2067980021170.1962828528160.1354540724990.20255235460.0743233995056-0.1610959147090.1125174061660.4445965197320.01111665372390.02582032225050.4412762028360.09205413374510.32382955911413.8815074715-26.338649839448.0152246779
125.61439397058-1.380734378571.367218332222.26768597336-0.2764944376853.714264775520.339506763203-0.538427452285-0.2878353209860.1442221237420.1232203657260.053644302640.504176431604-0.123486276716-0.1855251707120.4921327939780.003874573601510.05839350804640.4391697078660.01552134734880.3769759968298.46227808329-26.204429979843.5186138535
135.90451464709-1.295345429171.178623224771.395810292150.05598256176433.431758849190.244468198981-0.191191466146-0.6247430349940.1731295027170.09650524615230.1202522507850.6118179028340.0965081441837-0.2481635530440.4795988861280.1007790642890.02623545814590.5020823698130.04640249624150.42010497271912.1611534593-35.47221436544.3245922937
144.83835914858-0.271149815431.418553360471.728388336670.6507241947993.31457058297-0.002686348164860.0932769003954-0.3736718423860.1238568559610.1660727464210.1207351353470.06463274327810.18888506722-0.1509692900180.3377311584720.04286722424190.01484891225230.3410111911820.07213611710080.3422096238668.89845408693-28.544551147739.2571680544
152.54879113296-1.524628433162.988037444152.08027755015-0.6316485971464.776341158670.036071675779-0.718431187752-0.8998458996510.5882533839570.2378841022460.8031083544140.727770349367-0.367565234342-0.272025398820.5341694607520.0002537870409120.05856635456230.4264779920230.08941824106210.5196764531344.99392813537-35.041711665446.5886729489
163.74449661636-0.8262766976960.5972667287583.497218249122.226916594824.26072353866-0.355289716956-0.654736297072-0.2183219714050.7223165862130.2431373035770.786386438239-0.313625170667-0.5146050190750.3986283713410.612019961790.0615103235350.182073293010.6112449736320.1401203074290.5577349929351.78089465239-28.92224428950.2013540284
177.05526594411-0.561027951255-2.724677245713.37244027906-1.37932205841.943694841650.2646592120920.5942377342150.640616848184-0.128928752617-0.2648041546560.0607528547494-0.28707419474-0.121681405892-0.02478616446120.485513579420.1460063330620.01434439510030.6533557598640.05295716115660.34304817318931.9822333498-27.606716440113.6834930975
187.484035455610.0939163295478-2.559791532432.803076217630.3711002186476.36931127521-0.1498060470410.335110115688-0.4266644636340.130379249672-0.1293024227260.1217702350890.378205260662-0.576803905060.2852265626650.5212389549520.067973853728-0.03840600052480.4840997839220.0009347474967040.44128312028932.8202268736-38.571006662619.9778607974
193.69946570411-1.53851249457-1.010462170921.032937326920.6293546573241.244366388060.1976936810450.730550382537-0.296742546888-0.15707667783-0.2464462841920.1262475896350.107468348275-0.1199229274270.0578775541460.4299182142660.0522525624142-0.03672984044730.5321513839899.14006448604E-50.34960252729326.4587721458-32.984533929817.8816053759
203.823090282-0.3615322084251.291071594325.25242230123.046493825755.26445378001-0.217355328543-0.326712880036-0.1199066736230.8181521535180.005773839149290.3363954170430.354947477827-0.6723278029520.1133070447510.4137450915110.03311483924230.04526632725440.4755839608930.03261989657230.4674064168038.1691454395-18.389520031346.2513156572
212.07518208828-0.278667946880.7786428559395.705785742473.353967955824.88503218452-0.029066242080.2275063116240.318763586887-0.1888349544430.003493220549390.02268267146120.02785209892520.0282805295286-0.05541094292620.31221260046-0.03782170375480.02594732232320.5037993108140.0588250275810.41743285053112.8477782819-19.68771210730.1111248219
222.97755050076-0.4607294258640.3603312909835.350354557383.148747696768.536738024990.00561454717988-0.03214844614260.3521156459950.09449424900310.1321800228340.296991301509-0.737374313633-0.0378570956193-0.01261408087010.320191586217-0.002887423592030.04531689172240.4289626714180.07380659784960.4621597783337.37375745664-10.872616816438.2185916259
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'E' and (resid 334 through 380 )EA334 - 3801 - 47
22chain 'E' and (resid 381 through 459 )EA381 - 45948 - 126
33chain 'E' and (resid 460 through 494 )EA460 - 494127 - 161
44chain 'E' and (resid 495 through 527 )EA495 - 527162 - 194
55chain 'A' and (resid 1 through 17 )AE1 - 171 - 17
66chain 'A' and (resid 18 through 39 )AE18 - 3918 - 39
77chain 'A' and (resid 40 through 59 )AE40 - 5940 - 59
88chain 'A' and (resid 60 through 72 )AE60 - 7260 - 72
99chain 'A' and (resid 73 through 90 )AE73 - 9073 - 90
1010chain 'A' and (resid 91 through 113 )AE91 - 11391 - 113
1111chain 'A' and (resid 114 through 138 )AE114 - 138114 - 133
1212chain 'A' and (resid 139 through 149 )AE139 - 149134 - 144
1313chain 'A' and (resid 150 through 161 )AE150 - 161145 - 156
1414chain 'A' and (resid 162 through 193 )AE162 - 193157 - 188
1515chain 'A' and (resid 194 through 207 )AE194 - 207189 - 202
1616chain 'A' and (resid 208 through 220 )AE208 - 220203 - 215
1717chain 'B' and (resid 1 through 30 )BH1 - 301 - 30
1818chain 'B' and (resid 31 through 49 )BH31 - 4931 - 49
1919chain 'B' and (resid 50 through 121 )BH50 - 12150 - 121
2020chain 'B' and (resid 122 through 137 )BH122 - 137122 - 137
2121chain 'B' and (resid 138 through 174 )BH138 - 174138 - 174
2222chain 'B' and (resid 175 through 213 )BH175 - 213175 - 213

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