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- PDB-7neh: Crystal structure of the receptor binding domain of SARS-CoV-2 Sp... -

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Basic information

Entry
Database: PDB / ID: 7neh
TitleCrystal structure of the receptor binding domain of SARS-CoV-2 Spike glycoprotein in complex with COVOX-269 Fab
Components
  • COVOX-269 Fab heavy chain
  • COVOX-269 fab light chain
  • Spike glycoproteinSpike protein
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / SARS-CoV-2 B.1.1.7 variant / N501Y mutation / antibody / receptor-binding-domain / spike / neutralisation / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell ...Maturation of spike protein / viral translation / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / host extracellular space / suppression by virus of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / entry receptor-mediated virion attachment to host cell / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / receptor ligand activity / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Spike glycoprotein, N-terminal domain superfamily / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike glycoprotein, betacoronavirus / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal
Similarity search - Domain/homology
NITRATE ION / DI(HYDROXYETHYL)ETHER / Spike glycoprotein
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.77 Å
AuthorsZhou, D. / Ren, J. / Stuart, D.
Funding support United Kingdom, China, 2items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MR/N00065X/1 United Kingdom
CAMS Innovation Fund for Medical Sciences (CIFMS)2018-I2M-2-002 China
CitationJournal: Cell / Year: 2021
Title: Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera.
Authors: Supasa, P. / Zhou, D. / Dejnirattisai, W. / Liu, C. / Mentzer, A.J. / Ginn, H.M. / Zhao, Y. / Duyvesteyn, H.M.E. / Nutalai, R. / Tuekprakhon, A. / Wang, B. / Paesen, G.C. / Slon-Campos, J. / ...Authors: Supasa, P. / Zhou, D. / Dejnirattisai, W. / Liu, C. / Mentzer, A.J. / Ginn, H.M. / Zhao, Y. / Duyvesteyn, H.M.E. / Nutalai, R. / Tuekprakhon, A. / Wang, B. / Paesen, G.C. / Slon-Campos, J. / Lopez-Camacho, C. / Hallis, B. / Coombes, N. / Bewley, K.R. / Charlton, S. / Walter, T.S. / Barnes, E. / Dunachie, S.J. / Skelly, D. / Lumley, S.F. / Baker, N. / Shaik, I. / Humphries, H.E. / Godwin, K. / Gent, N. / Sienkiewicz, A. / Dold, C. / Levin, R. / Dong, T. / Pollard, A.J. / Knight, J.C. / Klenerman, P. / Crook, D. / Lambe, T. / Clutterbuck, E. / Bibi, S. / Flaxman, A. / Bittaye, M. / Belij-Rammerstorfer, S. / Gilbert, S. / Hall, D.R. / Williams, M.A. / Paterson, N.G. / James, W. / Carroll, M.W. / Fry, E.E. / Mongkolsapaya, J. / Ren, J. / Stuart, D.I. / Screaton, G.R.
History
DepositionFeb 4, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 3, 2021Provider: repository / Type: Initial release
Revision 1.1Mar 31, 2021Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Apr 28, 2021Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first
Revision 1.3Jan 31, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: COVOX-269 Fab heavy chain
L: COVOX-269 fab light chain
E: Spike glycoprotein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,18229
Polymers70,0083
Non-polymers2,17326
Water8,935496
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11710 Å2
ΔGint41 kcal/mol
Surface area27880 Å2
MethodPISA
Unit cell
Length a, b, c (Å)82.812, 149.712, 145.906
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Components on special symmetry positions
IDModelComponents
11L-544-

HOH

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Components

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Antibody , 2 types, 2 molecules HL

#1: Antibody COVOX-269 Fab heavy chain


Mass: 23557.420 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody COVOX-269 fab light chain


Mass: 23299.961 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Protein / Sugars , 2 types, 2 molecules E

#3: Protein Spike glycoprotein / Spike protein / S glycoprotein / E2 / Peplomer protein


Mass: 23150.891 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#4: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE

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Non-polymers , 6 types, 521 molecules

#5: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 19 / Source method: obtained synthetically / Formula: C2H6O2
#6: Chemical ChemComp-NO3 / NITRATE ION / Nitrate


Mass: 62.005 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: NO3
#7: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#8: Chemical ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#9: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#10: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 496 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.23 Å3/Da / Density % sol: 61.92 %
Crystal growTemperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: 0.09 M NPS, 0.1 M sodium HEPES and MOPS, 30% EDO_P8K

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Feb 2, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 1.77→75 Å / Num. obs: 88349 / % possible obs: 100 % / Redundancy: 13.5 % / Biso Wilson estimate: 37.24 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.091 / Rpim(I) all: 0.025 / Net I/σ(I): 14
Reflection shellResolution: 1.77→1.8 Å / Mean I/σ(I) obs: 0.3 / Num. unique obs: 4396 / CC1/2: 0.33

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Processing

Software
NameVersionClassification
GDA1.19_4092data collection
PHENIX1.19_4092refinement
xia2data reduction
xia2data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7BEM

7bem


Resolution: 1.77→42.74 Å / SU ML: 0.2787 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 24.2451
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1982 4325 4.91 %
Rwork0.1882 83704 -
obs0.1887 88029 99.66 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 49.8 Å2
Refinement stepCycle: LAST / Resolution: 1.77→42.74 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4820 0 139 496 5455
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0035056
X-RAY DIFFRACTIONf_angle_d0.6486831
X-RAY DIFFRACTIONf_chiral_restr0.0459759
X-RAY DIFFRACTIONf_plane_restr0.0048873
X-RAY DIFFRACTIONf_dihedral_angle_d13.01691806
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.77-1.790.3961080.40432588X-RAY DIFFRACTION92.01
1.79-1.810.36981570.37922710X-RAY DIFFRACTION98.9
1.81-1.830.40831340.36682779X-RAY DIFFRACTION99.69
1.83-1.860.35151240.34532804X-RAY DIFFRACTION99.97
1.86-1.880.32561370.33612768X-RAY DIFFRACTION100
1.88-1.910.32821210.31872808X-RAY DIFFRACTION100
1.91-1.930.32581640.31292744X-RAY DIFFRACTION99.83
1.93-1.960.33441510.30472746X-RAY DIFFRACTION100
1.96-1.990.28741250.2812817X-RAY DIFFRACTION99.97
1.99-2.030.30631440.25152772X-RAY DIFFRACTION99.97
2.03-2.060.26431130.24432786X-RAY DIFFRACTION100
2.06-2.10.27451330.22492808X-RAY DIFFRACTION100
2.1-2.140.21161580.20822776X-RAY DIFFRACTION100
2.14-2.180.24281510.20282759X-RAY DIFFRACTION100
2.18-2.230.21871590.20882776X-RAY DIFFRACTION100
2.23-2.280.20631400.20442778X-RAY DIFFRACTION100
2.28-2.340.22661460.20172796X-RAY DIFFRACTION100
2.34-2.40.20731550.20592760X-RAY DIFFRACTION100
2.4-2.470.20951370.20742796X-RAY DIFFRACTION100
2.47-2.550.23551700.20882763X-RAY DIFFRACTION100
2.55-2.640.24951500.20052817X-RAY DIFFRACTION100
2.64-2.750.22461420.19762806X-RAY DIFFRACTION100
2.75-2.870.19631500.19182772X-RAY DIFFRACTION100
2.87-3.030.21591380.19522819X-RAY DIFFRACTION100
3.03-3.220.2151500.19522830X-RAY DIFFRACTION100
3.22-3.460.20711400.17822824X-RAY DIFFRACTION99.97
3.46-3.810.18531340.16612847X-RAY DIFFRACTION100
3.81-4.360.14821350.14652851X-RAY DIFFRACTION100
4.36-5.50.13871970.13212824X-RAY DIFFRACTION100
5.5-42.740.16021620.16922980X-RAY DIFFRACTION99.71
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.513856426633-0.867407560718-1.375188454562.442933961453.678120903795.667815670510.0780782360709-1.055359617990.859217276863-0.577500012024-0.4718073783720.157322977888-2.16490219808-0.2930145303520.05761029783850.9500336460630.1376424333650.08669266730590.68990547499-0.08555065779850.837182102236-4.7829905886777.085337528419.2298727069
26.49298186041-3.04481317016-1.476406127348.196491552183.021700644185.28896619020.3891724355120.2151941989870.523438601832-1.20276117570.0200974837099-0.305988882474-0.9407449739280.198301401971-0.2509733508030.484010492625-0.04393965143760.08722454974380.285335017790.007962218061550.4379151105023.2360849055167.914029162710.6845878106
32.630645635190.5245796707551.1618061852.668474202733.775751244845.856744053110.0424917825552-0.734517589020.315844330426-0.131809521145-0.5428948004440.788139171147-0.563716487788-1.487644619560.4403327648540.51305612740.1328602554360.04792457433730.841110087941-0.06555382459550.633510027985-10.757103369666.97516417922.5182139047
44.02732330903-0.896761835079-0.8187692462123.927427032541.905209846274.157037425110.110825472672-0.131584212603-0.125174064019-0.121903712009-0.125023354108-0.0146456907235-0.128684202198-0.234820395695-0.07680556296940.309060608367-0.01211325703190.02834559125720.3695702949-0.01052193870830.419078592598-0.090100761398455.605378126612.0544884674
53.46097342342-1.156744504-0.4709024841284.730499290911.459763746862.874521501990.1871178458720.233767203668-0.0328099089868-0.6688356571590.0655508471065-0.483097753407-0.4384734092520.0832469311743-0.2493759524270.345567231817-0.05301555512380.08232818308910.268108512829-0.02046777600110.3912718496795.3853561057356.42924592315.26387695514
62.86582878501-0.6611813797853.750983822845.34659598867-1.902532094216.40902991418-0.155552064903-0.5002528778110.07115012227940.1024769643690.4584665021480.380398880119-0.645376516632-0.758953405592-0.2282208599450.5471499025150.1300368587040.09455490132910.9436526716490.02711623440840.581904216536-0.4401058409471.210033521732.614452804
70.7007169763171.15402647665-0.2185229795914.306240516990.6261909076891.831268838960.0814979277093-0.0668112282783-0.01535907398650.09759067992340.0795845753486-0.6189873721710.03043543841640.193504255427-0.1213438238310.270141479116-0.0244384156562-0.03856969096470.353185830026-0.009502229258270.4606139447156.2979808584230.3973912793.74321371329
82.40479489641-0.242856113683-0.4591432905732.24003740967-0.8301457771531.49633306156-0.05063233900020.406334816194-0.144662897185-0.416810982033-0.02075300195210.05045372338220.3324979713120.02141001625920.06373416699130.279328867263-0.01394582104780.03545428259050.2900640547740.02244689198340.221532114663-8.595989664832.17598293591-15.3525287076
91.50679648697-0.153568249684-1.038257239462.152529062631.115325726995.32244686899-0.05336790896410.0650203481612-0.117672995686-0.265848157971-0.0527400541332-0.05422350546590.248044756330.2225597754280.1232535422930.3129437150880.01558376949990.03464073513010.3206254739610.03185888778570.235017957947-3.159574227881.55803456666-18.2959342584
101.65513081052-0.741103728733-0.002326280421956.349021920422.720743485264.93141175497-0.001064664486210.250513942443-0.0446861478715-0.660349314887-0.2876059575110.0190958988783-0.289540389632-0.7187339459490.1571370577320.509277877667-0.0236940517134-0.009074257031650.4367144953130.06746291386280.378684672863-4.699135092136.9341372365-21.2599499604
110.813474342542-0.100998681979-0.2658866871863.708806425231.774129619673.354442488040.02499235837860.1284683200560.0654094931221-0.783979480870.0498270279988-0.453059818471-0.433800795885-0.138117433508-0.04364916446620.531438311804-0.05230364154050.1151337824570.3797092736540.03975053300390.4692656665063.1967907897641.6680802606-14.944794527
120.1929217157870.7239040590640.7612960640163.563840582341.501150823554.67462010346-0.09512542828720.2642890608690.258572982503-1.304266288660.132933869898-0.826419453519-0.6158594334010.409619879853-0.05423282594360.778527132898-0.1148093589450.1688723851420.3393666897680.05674881952160.4519598560353.902538242238.1466196537-24.0741164933
130.8208126753990.9457289470260.8402556001593.197003842081.267660092530.841701819199-0.0239349988888-0.01814151117810.118862312902-0.4294097215070.0104570983573-0.0109166573232-0.223472501216-0.1420312802510.04715952120910.417429808516-0.003259912420370.06816156530430.3606399630690.05279243467410.366341591064-3.2031824563633.8545646669-16.0905232933
146.719858269771.85295810249-0.9839684057455.50757043001-0.895605357656.28307576570.176963682487-0.228345378142-0.441862647541-0.166181977354-0.1391949100320.0002953384772260.786368958519-0.02911281196680.01493008599770.3618325885550.00105804165309-0.03911381712420.28066038168-0.0286391974340.276543213344-15.7611520505-0.656346402381-18.6958117754
153.778211331832.17075802608-3.039902562813.9855106589-2.083621429734.042662379730.1999911431940.1110260751420.310297921594-0.245630619632-0.2097471252410.0937366431638-0.3229578581580.1602746986620.006186764086980.435465468222-0.000100711637063-0.0524708871040.3501366967040.002439970579680.249241525518-15.087918925313.9031477484-23.4776417687
168.035307957680.585197357619-0.3416441750571.72329059276-0.2990707421960.7991894781280.0463354722270.1795555347650.119123689463-0.102274823951-0.0005721694664710.274426143568-0.184526351991-0.101329455111-0.01735216726110.4194216690510.0601157975352-0.01408084243520.382233296328-0.01888786727670.299699831568-21.480765532613.0189148437-16.8485684554
179.109356397134.36988074007-2.489134352064.34798843854-4.290832917854.930005315830.06571725505780.255506062014-0.175405405053-0.4989420159180.0696629885242-0.533478694020.06154509548050.26356485042-0.03595455936510.566770509105-0.022473612340.05913829106510.3691498933360.0213756353340.371491096967-0.74144091692918.1487779299-27.4251109267
185.885196852674.29216632465-2.208064006926.51213457531-3.177213996024.242353187720.02311758169120.2612116450480.0443003687066-0.3360491479080.1328422095960.4583082557410.0894824108914-0.373000166371-0.1241516817350.3481297525120.0790786216961-0.03917686775280.347058430876-0.05922797260320.303523186677-22.49529568266.90283457767-22.5035832591
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'E' and (resid 332 through 342 )ER332 - 3421 - 11
22chain 'E' and (resid 343 through 358 )ER343 - 35812 - 27
33chain 'E' and (resid 359 through 393 )ER359 - 39328 - 62
44chain 'E' and (resid 394 through 421 )ER394 - 42163 - 90
55chain 'E' and (resid 422 through 516 )ER422 - 51691 - 185
66chain 'E' and (resid 517 through 527 )ER517 - 527186 - 196
77chain 'H' and (resid 1 through 113 )HA1 - 1131 - 113
88chain 'H' and (resid 114 through 138 )HA114 - 138114 - 137
99chain 'H' and (resid 139 through 220 )HA139 - 220138 - 219
1010chain 'L' and (resid 1 through 18 )LJ1 - 181 - 18
1111chain 'L' and (resid 19 through 69 )LJ19 - 6919 - 69
1212chain 'L' and (resid 70 through 83 )LJ70 - 8370 - 83
1313chain 'L' and (resid 84 through 114 )LJ84 - 11484 - 114
1414chain 'L' and (resid 115 through 129 )LJ115 - 129115 - 129
1515chain 'L' and (resid 130 through 151 )LJ130 - 151130 - 151
1616chain 'L' and (resid 152 through 164 )LJ152 - 164152 - 164
1717chain 'L' and (resid 165 through 175 )LJ165 - 175165 - 175
1818chain 'L' and (resid 176 through 215 )LJ176 - 215176 - 215

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