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- PDB-7n6g: C1 of central pair -

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Basic information

Entry
Database: PDB / ID: 7n6g
TitleC1 of central pair
Components
  • (Unknown protein) x 15
  • CPC1
  • Calmodulin
  • DPY30
  • FAP101
  • FAP108
  • FAP114
  • FAP119
  • FAP15
  • FAP194
  • FAP216
  • FAP227
  • FAP246
  • FAP275
  • FAP279
  • FAP289
  • FAP297
  • FAP305
  • FAP360
  • FAP42
  • FAP46
  • FAP47
  • FAP54
  • FAP69
  • FAP7
  • FAP74
  • FAP76
  • FAP81
  • FAP92
  • FAP99
  • HTH_9 domain-containing protein
  • HYDIN
  • Heat shock protein 70AHeat shock response
  • PF16
  • PF6
  • Phosphopyruvate hydrataseEnolase
  • Tubulin alpha
  • Tubulin beta
KeywordsSTRUCTURAL PROTEIN / central pair
Function / homology
Function and homology information


axonemal central apparatus / axonemal central pair projection / : / axonemal central apparatus assembly / establishment of meiotic spindle localization / sperm axoneme assembly / positive regulation of flagellated sperm motility / sperm principal piece / cilium movement involved in cell motility / epithelial cilium movement involved in extracellular fluid movement ...axonemal central apparatus / axonemal central pair projection / : / axonemal central apparatus assembly / establishment of meiotic spindle localization / sperm axoneme assembly / positive regulation of flagellated sperm motility / sperm principal piece / cilium movement involved in cell motility / epithelial cilium movement involved in extracellular fluid movement / cilium movement / axoneme assembly / guanylate kinase activity / response to odorant / calcium-dependent cysteine-type endopeptidase activity / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / adenylate kinase / adenylate kinase activity / manchette / SRP-dependent cotranslational protein targeting to membrane, translocation / filopodium assembly / MLL3/4 complex / Set1C/COMPASS complex / non-motile cilium / motile cilium / neuron projection extension / spindle organization / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / axoneme / chaperone cofactor-dependent protein refolding / mitochondrial respiratory chain complex I / cilium assembly / subtelomeric heterochromatin formation / enzyme regulator activity / protein folding chaperone / sperm midpiece / heat shock protein binding / tubulin binding / acrosomal vesicle / electron transport chain / glycolytic process / ATP-dependent protein folding chaperone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cilium / structural constituent of cytoskeleton / microtubule cytoskeleton organization / spindle pole / microtubule cytoskeleton / mitotic cell cycle / protein refolding / microtubule binding / microtubule / chromosome, telomeric region / hydrolase activity / calmodulin binding / negative regulation of cell population proliferation / phosphorylation / GTPase activity / calcium ion binding / GTP binding / magnesium ion binding / ATP hydrolysis activity / proteolysis / ATP binding / membrane / metal ion binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Sperm-associated antigen 17 / Cilia- and flagella-associated protein 54 / Domain of unknown function DUF4456 / Domain of unknown function DUF4455 / Primary ciliary dyskinesia protein 1 / Flagellar C1a complex subunit C1a-32 / Deleted in lung and esophageal cancer protein 1 / Cilia- and flagella-associated protein 99 / Cilia- and flagella-associated protein 46 / Leucine-rich repeat-containing protein 72 ...Sperm-associated antigen 17 / Cilia- and flagella-associated protein 54 / Domain of unknown function DUF4456 / Domain of unknown function DUF4455 / Primary ciliary dyskinesia protein 1 / Flagellar C1a complex subunit C1a-32 / Deleted in lung and esophageal cancer protein 1 / Cilia- and flagella-associated protein 99 / Cilia- and flagella-associated protein 46 / Leucine-rich repeat-containing protein 72 / : / : / Domain of unknown function (DUF4455) / Domain of unknown function (DUF4456) / Flagellar C1a complex subunit C1a-32 / Cilia- and flagella-associated protein 54 / Cilia- and flagella-associated protein 69, ARM repeat / Hydrocephalus-inducing-like / Flagellar-associated PapD-like / CH-like domain in sperm protein / CH-like domain in sperm protein / Sdc1/DPY30 / Adenylate kinase, active site lid domain superfamily / Dpy-30 motif / Dpy-30 motif / Peptidase C2, calpain, catalytic domain / Calpain family cysteine protease / Cysteine proteinase, calpain-type, catalytic domain profile. / Calpain-like thiol protease family. / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / MORN motif / MORN repeat / Serine-threonine protein phosphatase, N-terminal / Serine-threonine protein phosphatase N-terminal domain / Transglutaminase/protease-like homologues / Transglutaminase-like / RNA polymerase III subunit RPC82-related, helix-turn-helix / RNA polymerase III subunit RPC82 helix-turn-helix domain / Adenylate kinase / Adenylate kinase, conserved site / Adenylate kinase signature. / Adenylate kinase/UMP-CMP kinase / Enolase / Enolase, conserved site / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal domain / Enolase signature. / Enolase, C-terminal TIM barrel domain / Enolase, N-terminal domain / Leucine rich repeat, ribonuclease inhibitor type / Leucine-rich repeat / Serine/threonine specific protein phosphatases signature. / Protein phosphatase 2A homologues, catalytic domain. / Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase / IQ calmodulin-binding motif / Guanylate kinase-like domain profile. / Guanylate kinase-like domain / Leucine-rich repeats, bacterial type / Heat shock hsp70 proteins family signature 2. / Heat shock hsp70 proteins family signature 1. / Heat shock hsp70 proteins family signature 3. / Heat shock protein 70, conserved site / Guanylate kinase/L-type calcium channel beta subunit / Guanylate kinase / Guanylate kinase homologues. / Heat shock protein 70kD, peptide-binding domain superfamily / Heat shock protein 70 family / Hsp70 protein / Calponin homology domain / Heat shock protein 70kD, C-terminal domain superfamily / Armadillo/beta-catenin-like repeat / Calponin homology (CH) domain / Leucine-rich repeat, SDS22-like subfamily / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / Short calmodulin-binding motif containing conserved Ile and Gln residues. / Enolase-like, N-terminal / Calponin homology domain / Enolase-like, C-terminal domain superfamily / CH domain superfamily / Calponin homology (CH) domain profile. / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / IQ motif profile. / Armadillo/beta-catenin-like repeats / Armadillo / Metallo-dependent phosphatase-like / IQ motif, EF-hand binding site / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Uncharacterized protein / Calponin-homology (CH) domain-containing protein / EF-hand domain-containing protein / Uncharacterized protein / adenylate kinase / Flagellar associated protein / HTH_9 domain-containing protein / Spermatogenesis-associated protein 17 ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Uncharacterized protein / Calponin-homology (CH) domain-containing protein / EF-hand domain-containing protein / Uncharacterized protein / adenylate kinase / Flagellar associated protein / HTH_9 domain-containing protein / Spermatogenesis-associated protein 17 / Flagellar associated protein / Sperm-associated antigen 6 / Flagellar associated protein / Flagellar associated protein / Flagellar associated protein / Cilia- and flagella-associated protein 69 ARM repeats domain-containing protein / Uncharacterized protein / Minichromosome loss protein Mcl1 middle region domain-containing protein / Cilia- and flagella-associated protein 46 / Calmodulin / Cilia- and flagella-associated protein 99 / Uncharacterized protein / Subunit of chromatin modifying protein / Cilia- and flagella-associated protein 54 / Uncharacterized protein / Flagellar associated coiled-coil protein / Uncharacterized protein / Uncharacterized protein / phosphopyruvate hydratase / Cilia- and flagella-associated protein 74 / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Central apparatus associated protein C1a-86 / Central apparatus associated protein C1a-34 / Central apparatus associated protein C1a-32 / Central apparatus associated protein C1a-18 / Central pair complex 1 / PF6 protein / Serine/threonine-protein phosphatase
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsHan, L. / Zhang, K.
Funding support United States, 1items
OrganizationGrant numberCountry
Other private United States
CitationJournal: Nat Struct Mol Biol / Year: 2022
Title: Cryo-EM structure of an active central apparatus.
Authors: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang /
Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
History
DepositionJun 8, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 18, 2022Provider: repository / Type: Initial release
Revision 1.1Jun 1, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
0A: PF16
0B: PF16
0C: PF16
0D: PF16
0E: PF16
0F: PF16
0G: PF16
0L: PF16
0M: PF16
0N: PF16
0O: PF16
0P: PF16
0Q: PF16
0R: PF16
0S: PF16
0T: PF16
0U: PF16
0V: PF16
0X: PF16
0Y: PF16
0Z: PF16
1A: PF16
1B: PF16
1C: PF16
1D: FAP194
1E: FAP194
1F: FAP194
1G: FAP194
1H: FAP69
1I: FAP69
1J: HYDIN
1K: HYDIN
1L: FAP47
1M: FAP47
1N: FAP46
1O: FAP54
1P: HTH_9 domain-containing protein
1Q: FAP297
1R: FAP108
1S: FAP108
1T: FAP76
1a: Tubulin beta
1b: Tubulin alpha
1c: Tubulin beta
1d: Tubulin alpha
1e: Tubulin beta
1f: Tubulin alpha
1g: Tubulin beta
1h: Tubulin alpha
2F: FAP275
2G: FAP275
2H: Unknown protein
2I: Unknown protein
2J: Unknown protein
2K: Unknown protein
2L: Unknown protein
2M: Unknown protein
2N: Unknown protein
2O: Unknown protein
2P: Unknown protein
2Q: Unknown protein
2R: Unknown protein
2S: FAP289
2T: FAP289
2U: FAP289
2V: FAP289
2W: Unknown protein
2X: Unknown protein
2Y: Unknown protein
2Z: Unknown protein
2a: Tubulin beta
2b: Tubulin alpha
2c: Tubulin beta
2d: Tubulin alpha
2e: Tubulin beta
2f: Tubulin alpha
2g: Tubulin beta
2h: Tubulin alpha
3A: Unknown protein
3B: Unknown protein
3C: FAP216
3E: FAP92
3F: FAP99
3G: FAP99
3H: FAP74
3I: FAP74
3J: FAP360
3K: FAP360
3L: FAP279
3M: FAP7
3N: FAP7
3O: FAP7
3P: FAP7
3Q: FAP7
3R: FAP7
3S: FAP7
3T: FAP7
3U: FAP7
3V: FAP7
3W: FAP7
3X: FAP7
3Y: FAP7
3Z: FAP7
3a: Tubulin beta
3b: Tubulin alpha
3c: Tubulin beta
3d: Tubulin alpha
3e: Tubulin beta
3f: Tubulin alpha
3g: Tubulin beta
3h: Tubulin alpha
4A: FAP7
4B: FAP7
4C: FAP81
4D: FAP15
4E: PF6
4F: PF6
4G: PF6
4H: PF6
4I: DPY30
4J: DPY30
4K: DPY30
4L: DPY30
4M: DPY30
4N: DPY30
4O: DPY30
4P: DPY30
4Q: FAP305
4R: FAP305
4S: FAP305
4T: FAP101
4U: FAP101
4V: FAP101
4W: FAP227
4X: FAP227
4Y: FAP227
4Z: FAP227
4a: Tubulin beta
4b: Tubulin alpha
4c: Tubulin beta
4d: Tubulin alpha
4e: Tubulin beta
4f: Tubulin alpha
4g: Tubulin beta
4h: Tubulin alpha
5A: FAP227
5B: FAP227
5C: FAP227
5D: FAP227
5E: FAP114
5F: FAP114
5G: FAP114
5H: FAP114
5I: FAP119
5J: FAP119
5K: FAP119
5L: FAP119
5M: Calmodulin
5N: Calmodulin
5O: Calmodulin
5Q: Calmodulin
5R: Calmodulin
5S: Calmodulin
5T: Calmodulin
5U: Calmodulin
5V: Calmodulin
5W: Unknown protein
5X: Unknown protein
5Y: Unknown protein
5Z: Unknown protein
5a: Tubulin beta
5b: Tubulin alpha
5c: Tubulin beta
5d: Tubulin alpha
5e: Tubulin beta
5f: Tubulin alpha
5g: Tubulin beta
5h: Tubulin alpha
6A: CPC1
6B: CPC1
6D: FAP69
6E: FAP246
6F: FAP246
6G: Phosphopyruvate hydratase
6H: Phosphopyruvate hydratase
6I: Phosphopyruvate hydratase
6J: Phosphopyruvate hydratase
6K: Heat shock protein 70A
6L: Heat shock protein 70A
6M: FAP42
6N: FAP42
6O: Unknown protein
6P: Unknown protein
6Q: Unknown protein
6a: Tubulin beta
6b: Tubulin alpha
6c: Tubulin beta
6d: Tubulin alpha
6e: Tubulin beta
6f: Tubulin alpha
6g: Tubulin beta
6h: Tubulin alpha
7a: Tubulin beta
7b: Tubulin alpha
7c: Tubulin beta
7d: Tubulin alpha
7e: Tubulin beta
7f: Tubulin alpha
7g: Tubulin beta
7h: Tubulin alpha
8a: Tubulin beta
8b: Tubulin alpha
8c: Tubulin beta
8d: Tubulin alpha
8e: Tubulin beta
8f: Tubulin alpha
8g: Tubulin beta
8h: Tubulin alpha
9a: Tubulin beta
9b: Tubulin alpha
9c: Tubulin beta
9d: Tubulin alpha
9e: Tubulin beta
9f: Tubulin alpha
9g: Tubulin beta
9h: Tubulin alpha
Aa: Tubulin beta
Ab: Tubulin alpha
Ac: Tubulin beta
Ad: Tubulin alpha
Ae: Tubulin beta
Af: Tubulin alpha
Ag: Tubulin beta
Ah: Tubulin alpha
Ba: Tubulin beta
Bb: Tubulin alpha
Bc: Tubulin beta
Bd: Tubulin alpha
Be: Tubulin beta
Bf: Tubulin alpha
Bg: Tubulin beta
Bh: Tubulin alpha
Ca: Tubulin beta
Cb: Tubulin alpha
Cc: Tubulin beta
Cd: Tubulin alpha
Ce: Tubulin beta
Cf: Tubulin alpha
Cg: Tubulin beta
Ch: Tubulin alpha
Da: Tubulin beta
Db: Tubulin alpha
Dc: Tubulin beta
Dd: Tubulin alpha
De: Tubulin beta
Df: Tubulin alpha
Dg: Tubulin beta
Dh: Tubulin alpha
0H: PF16
0J: PF16
0W: PF16
0I: PF16
0K: PF16
5P: Calmodulin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,146,022368
Polymers16,095,770264
Non-polymers50,252104
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 49 types, 260 molecules 0A0B0C0D0E0F0G0L0M0N0O0P0Q0R0S0T0U0V0X0Y0Z1A1B1C0H0J0W0I0K1D...

#1: Protein ...
PF16


Mass: 54710.590 Da / Num. of mol.: 29 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DEG1
#2: Protein
FAP194


Mass: 57764.508 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D452
#3: Protein FAP69


Mass: 114362.844 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DWV9
#4: Protein HYDIN


Mass: 425814.750 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E6E5
#5: Protein FAP47 / Calponin-homology (CH) domain-containing protein


Mass: 319999.812 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CP55
#6: Protein FAP46 / Cilia- and flagella-associated protein 46


Mass: 289610.406 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8ICS9
#7: Protein FAP54 / Flagella-associated protein 54


Mass: 332696.375 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J666
#8: Protein HTH_9 domain-containing protein


Mass: 109413.945 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D8D9
#9: Protein FAP297


Mass: 99187.336 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3E6N2
#10: Protein FAP108


Mass: 47789.062 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DQN7
#11: Protein FAP76


Mass: 172707.297 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DDV4
#12: Protein ...
Tubulin beta / Tubulin beta-1/beta-2 chain / Beta-tubulin


Mass: 49665.809 Da / Num. of mol.: 52 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P04690
#13: Protein ...
Tubulin alpha / Tubulin alpha-1 chain


Mass: 49638.008 Da / Num. of mol.: 52 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: P09204
#14: Protein FAP275 / Flagellar associated protein


Mass: 18171.365 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J870
#15: Protein Unknown protein


Mass: 16698.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#16: Protein Unknown protein


Mass: 9209.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#17: Protein Unknown protein


Mass: 8528.504 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#18: Protein Unknown protein


Mass: 4613.678 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#20: Protein Unknown protein


Mass: 4443.468 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#22: Protein Unknown protein


Mass: 9039.134 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#23: Protein Unknown protein


Mass: 9379.553 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#24: Protein
FAP289 / Flagellar associated coiled-coil protein


Mass: 46325.992 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JCZ9
#25: Protein Unknown protein


Mass: 14230.525 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#26: Protein Unknown protein


Mass: 5549.833 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#28: Protein FAP216 / Flagellar associated protein


Mass: 79183.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JGM3
#29: Protein FAP92


Mass: 153728.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CZ11
#30: Protein FAP99 / Cilia- and flagella-associated protein 99


Mass: 90340.258 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IUG5
#31: Protein FAP74 / Flagella-associated protein 74 / Cilia- and flagella-associated protein 74


Mass: 204036.094 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: D4P3R7
#32: Protein FAP360


Mass: 80773.195 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CMR7
#33: Protein FAP279


Mass: 43267.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DN67
#34: Protein
FAP7 / Flagella associated protein


Mass: 54721.914 Da / Num. of mol.: 16 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IVW2
#35: Protein FAP81


Mass: 234483.781 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3DQM4
#36: Protein FAP15 / Serine/threonine-protein phosphatase


Mass: 34888.031 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant)
References: UniProt: Q9XGU3, protein-serine/threonine phosphatase
#37: Protein
PF6 / PF6 protein / Flagellar central pair-associated protein


Mass: 237723.016 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q9ATK5
#38: Protein
DPY30 / Subunit of chromatin modifying protein


Mass: 11494.096 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8J1X7
#39: Protein FAP305


Mass: 47031.668 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D8V0
#40: Protein FAP101 / Central apparatus associated protein C1a-86


Mass: 85796.789 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q45QX3
#41: Protein
FAP227 / Central apparatus associated protein C1a-18


Mass: 19550.742 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q45QX6
#42: Protein
FAP114 / Flagellar associated protein C1a-32 / Central apparatus associated protein C1a-32


Mass: 31575.461 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q45QX5
#43: Protein
FAP119 / Flagellar associated protein / Central apparatus associated protein C1a-34


Mass: 33850.367 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q45QX4
#44: Protein
Calmodulin /


Mass: 18317.215 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8IDP6
#45: Protein Unknown protein


Mass: 11677.386 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#46: Protein Unknown protein


Mass: 15926.566 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#47: Protein CPC1 / Adenylate kinase


Mass: 205400.188 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: Q6J4H1, adenylate kinase
#48: Protein FAP246


Mass: 120452.891 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3CXT1
#49: Protein
Phosphopyruvate hydratase / Enolase


Mass: 51664.762 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JH98, phosphopyruvate hydratase
#50: Protein Heat shock protein 70A / Heat shock response


Mass: 71303.758 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A8JEU4, EC: 3.6.1.3
#51: Protein FAP42 / Adenylate kinase


Mass: 269172.500 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / References: UniProt: A0A2K3D425, adenylate kinase
#52: Protein Unknown protein


Mass: 7782.416 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124

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Protein/peptide , 3 types, 4 molecules 2M2O3A3B

#19: Protein/peptide Unknown protein


Mass: 1549.902 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#21: Protein/peptide Unknown protein


Mass: 4017.944 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124
#27: Protein/peptide Unknown protein


Mass: 3081.790 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chlamydomonas reinhardtii (plant) / Strain: cc124

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Non-polymers , 2 types, 104 molecules

#53: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE / Guanosine diphosphate


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 52 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#54: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate


Mass: 523.180 Da / Num. of mol.: 52 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: C1 microtubule and its associated projection of central pair
Type: COMPLEX / Entity ID: #1-#52 / Source: NATURAL
Source (natural)Organism: Chlamydomonas reinhardtii (plant) / Strain: cc124
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2500 nm / Nominal defocus min: 1200 nm
Image recordingElectron dose: 38.6 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K2 QUANTUM (4k x 4k)

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Processing

Software
NameVersionClassification
phenix.real_space_refine1.17_3644refinement
PHENIX1.17_3644refinement
CTF correctionType: NONE
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 190727 / Symmetry type: POINT
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 103.11 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0084808568
ELECTRON MICROSCOPYf_angle_d0.95991099094
ELECTRON MICROSCOPYf_chiral_restr0.057125276
ELECTRON MICROSCOPYf_plane_restr0.0062144707
ELECTRON MICROSCOPYf_dihedral_angle_d14.5349115317

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