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- PDB-7ler: Netrin-1 filament assembly -

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Basic information

Entry
Database: PDB / ID: 7ler
TitleNetrin-1 filament assembly
ComponentsNetrin-1
KeywordsSIGNALING PROTEIN / heparin binding protein / filament
Function / homology
Function and homology information


regulation of glial cell migration / chemorepulsion of axon / anterior/posterior axon guidance / B cell mediated immunity / Cdc42 protein signal transduction / T cell mediated immunity / motor neuron migration / tissue development / negative regulation of axon extension / substrate-dependent cell migration, cell extension ...regulation of glial cell migration / chemorepulsion of axon / anterior/posterior axon guidance / B cell mediated immunity / Cdc42 protein signal transduction / T cell mediated immunity / motor neuron migration / tissue development / negative regulation of axon extension / substrate-dependent cell migration, cell extension / motor neuron axon guidance / positive regulation of cell motility / nuclear migration / regulation of synapse assembly / inner ear morphogenesis / B cell proliferation / dendrite development / basement membrane / positive regulation of axon extension / glial cell proliferation / positive regulation of glial cell proliferation / substrate adhesion-dependent cell spreading / cytokine activity / cell periphery / animal organ morphogenesis / cell-cell adhesion / actin cytoskeleton / Ras protein signal transduction / extracellular region / nucleoplasm / cytosol
Similarity search - Function
Laminin, N-terminal / Laminin N-terminal (Domain VI) / Laminin N-terminal domain profile. / Laminin N-terminal domain (domain VI) / Laminin-type EGF-like (LE) domain profile. / Laminin-type EGF-like (LE) domain signature. / Laminin-type epidermal growth factor-like domai / Laminin EGF domain / Laminin-type EGF domain / Netrin C-terminal Domain ...Laminin, N-terminal / Laminin N-terminal (Domain VI) / Laminin N-terminal domain profile. / Laminin N-terminal domain (domain VI) / Laminin-type EGF-like (LE) domain profile. / Laminin-type EGF-like (LE) domain signature. / Laminin-type epidermal growth factor-like domai / Laminin EGF domain / Laminin-type EGF domain / Netrin C-terminal Domain / Netrin module, non-TIMP type / UNC-6/NTR/C345C module / Netrin domain / NTR domain profile. / Tissue inhibitor of metalloproteinases-like, OB-fold / EGF-like domain signature 1. / Galactose-binding-like domain superfamily
Similarity search - Domain/homology
Biological speciesGallus gallus (chicken)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 5.99 Å
AuthorsMcDougall, M. / Gupta, M. / Stetefeld, J.
Funding support Canada, 1items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)RPA-109759 Canada
CitationJournal: Nat Commun / Year: 2023
Title: The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation.
Authors: Meier, M. / Gupta, M. / Akgul, S. / McDougall, M. / Imhof, T. / Nikodemus, D. / Reuten, R. / Moya-Torres, A. / To, V. / Ferens, F. / Heide, F. / Padilla-Meier, G.P. / Kukura, P. / Huang, W. ...Authors: Meier, M. / Gupta, M. / Akgul, S. / McDougall, M. / Imhof, T. / Nikodemus, D. / Reuten, R. / Moya-Torres, A. / To, V. / Ferens, F. / Heide, F. / Padilla-Meier, G.P. / Kukura, P. / Huang, W. / Gerisch, B. / Morgelin, M. / Poole, K. / Antebi, A. / Koch, M. / Stetefeld, J.
History
DepositionJan 14, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Mar 15, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Aug 16, 2023Group: Data collection / Other
Category: chem_comp_atom / chem_comp_bond / pdbx_database_status
Item: _pdbx_database_status.pdb_format_compatible
Revision 1.3Oct 25, 2023Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Netrin-1
B: Netrin-1
C: Netrin-1
E: Netrin-1
D: Netrin-1
F: Netrin-1
G: Netrin-1
H: Netrin-1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)410,86140
Polymers396,6398
Non-polymers14,22132
Water0
1
A: Netrin-1
B: Netrin-1
C: Netrin-1
E: Netrin-1
D: Netrin-1
F: Netrin-1
G: Netrin-1
H: Netrin-1
hetero molecules
x 6


  • defined by author
  • Evidence: scanning transmission electron microscopy, Negative Stain Electron Microscopy with 2D Alignments
  • 2.47 MDa, 48 polymers
Theoretical massNumber of molelcules
Total (without water)2,465,163240
Polymers2,379,83648
Non-polymers85,327192
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_344-y-2,x-y-1,z-1/31
crystal symmetry operation3_434-x+y-1,-x-2,z-2/31
crystal symmetry operation4_334-x-2,-y-2,z-1/21
crystal symmetry operation5_545y,-x+y-1,z+1/61
crystal symmetry operation6_454x-y-1,x,z-1/61
Unit cell
Length a, b, c (Å)196.690, 196.690, 476.330
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number170
Space group name H-MP65
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 40 through 255 or resid 272...
21(chain B and (resid 40 through 255 or resid 272...
31(chain C and (resid 40 through 255 or resid 272...
41(chain D and (resid 40 through 334 or (resid 335...
51(chain E and (resid 40 through 255 or resid 272...
61(chain F and (resid 40 through 255 or resid 272...
71(chain G and (resid 40 through 255 or resid 272...
81(chain H and (resid 40 through 255 or resid 272...
12chain I
22chain J
32chain K
42chain L
52chain M
62chain N
72chain O
82chain P

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resid 40 through 255 or resid 272...A40 - 255
121(chain A and (resid 40 through 255 or resid 272...A272 - 334
131(chain A and (resid 40 through 255 or resid 272...A335
141(chain A and (resid 40 through 255 or resid 272...A40 - 457
151(chain A and (resid 40 through 255 or resid 272...A40 - 457
161(chain A and (resid 40 through 255 or resid 272...A40 - 457
171(chain A and (resid 40 through 255 or resid 272...A40 - 457
211(chain B and (resid 40 through 255 or resid 272...B40 - 255
221(chain B and (resid 40 through 255 or resid 272...B272 - 334
231(chain B and (resid 40 through 255 or resid 272...B335
241(chain B and (resid 40 through 255 or resid 272...B40 - 457
251(chain B and (resid 40 through 255 or resid 272...B40 - 457
261(chain B and (resid 40 through 255 or resid 272...B40 - 457
271(chain B and (resid 40 through 255 or resid 272...B40 - 457
311(chain C and (resid 40 through 255 or resid 272...C40 - 255
321(chain C and (resid 40 through 255 or resid 272...C272 - 389
331(chain C and (resid 40 through 255 or resid 272...C390
341(chain C and (resid 40 through 255 or resid 272...C40 - 457
351(chain C and (resid 40 through 255 or resid 272...C40 - 457
361(chain C and (resid 40 through 255 or resid 272...C40 - 457
371(chain C and (resid 40 through 255 or resid 272...C40 - 457
411(chain D and (resid 40 through 334 or (resid 335...D40 - 334
421(chain D and (resid 40 through 334 or (resid 335...D335
431(chain D and (resid 40 through 334 or (resid 335...D40 - 457
441(chain D and (resid 40 through 334 or (resid 335...D40 - 457
451(chain D and (resid 40 through 334 or (resid 335...D40 - 457
461(chain D and (resid 40 through 334 or (resid 335...D40 - 457
511(chain E and (resid 40 through 255 or resid 272...E40 - 255
521(chain E and (resid 40 through 255 or resid 272...E272 - 334
531(chain E and (resid 40 through 255 or resid 272...E335
541(chain E and (resid 40 through 255 or resid 272...E40 - 457
551(chain E and (resid 40 through 255 or resid 272...E40 - 457
561(chain E and (resid 40 through 255 or resid 272...E40 - 457
571(chain E and (resid 40 through 255 or resid 272...E40 - 457
611(chain F and (resid 40 through 255 or resid 272...F40 - 255
621(chain F and (resid 40 through 255 or resid 272...F272 - 334
631(chain F and (resid 40 through 255 or resid 272...F335
641(chain F and (resid 40 through 255 or resid 272...F40 - 457
651(chain F and (resid 40 through 255 or resid 272...F40 - 457
661(chain F and (resid 40 through 255 or resid 272...F40 - 457
671(chain F and (resid 40 through 255 or resid 272...F40 - 457
711(chain G and (resid 40 through 255 or resid 272...G40 - 255
721(chain G and (resid 40 through 255 or resid 272...G272 - 334
731(chain G and (resid 40 through 255 or resid 272...G335
741(chain G and (resid 40 through 255 or resid 272...G40 - 457
751(chain G and (resid 40 through 255 or resid 272...G40 - 457
761(chain G and (resid 40 through 255 or resid 272...G40 - 457
771(chain G and (resid 40 through 255 or resid 272...G40 - 457
811(chain H and (resid 40 through 255 or resid 272...H40 - 255
821(chain H and (resid 40 through 255 or resid 272...H272 - 334
831(chain H and (resid 40 through 255 or resid 272...H335
841(chain H and (resid 40 through 255 or resid 272...H40 - 457
851(chain H and (resid 40 through 255 or resid 272...H40 - 457
861(chain H and (resid 40 through 255 or resid 272...H40 - 457
871(chain H and (resid 40 through 255 or resid 272...H40 - 457
112chain II501 - 531
212chain JJ501 - 531
312chain KK501 - 531
412chain LL501 - 531
512chain MM501 - 531
612chain NN501 - 531
712chain OO501 - 531
812chain PP501 - 531

NCS ensembles :
ID
1
2

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Components

#1: Protein
Netrin-1 /


Mass: 49579.926 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: NTN1 / Production host: Homo sapiens (human) / References: UniProt: Q90922
#2: Polysaccharide
alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 910.823 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/3,5,4/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3/a4-b1_b4-c1_c3-d1_c6-e1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}LINUCSPDB-CARE
#3: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#4: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 6.71 Å3/Da / Density % sol: 81.66 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 40% MPD, 100 mM Tris

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CLSI / Beamline: 08B1-1 / Wavelength: 0.97952 Å
DetectorType: RAYONIX MX300HE / Detector: CCD / Date: Sep 12, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97952 Å / Relative weight: 1
Reflection twinOperator: k,h,-l / Fraction: 0.5
ReflectionResolution: 5.99→49.17 Å / Num. obs: 26042 / % possible obs: 99.5 % / Redundancy: 11.1 % / CC1/2: 0.997 / Net I/σ(I): 8
Reflection shellResolution: 5.995→6.208 Å / Num. unique obs: 2565 / CC1/2: 0.777

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Processing

Software
NameVersionClassification
PHENIX1.18.2-3874_3874refinement
XSCALEdata scaling
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4OVE

4ove


Resolution: 5.99→49.17 Å / Cross valid method: THROUGHOUT / σ(F): 3.44 / Phase error: 38.74 / Stereochemistry target values: TWIN_LSQ_F
RfactorNum. reflection% reflection
Rfree0.3681 1336 5.13 %
Rwork0.3044 24692 -
obs0.3838 26028 99.94 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 1162.7 Å2 / Biso mean: 304.5544 Å2 / Biso min: 102.85 Å2
Refinement stepCycle: final / Resolution: 5.99→49.17 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms25469 0 936 0 26405
Biso mean--290.38 --
Num. residues----3249
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01427044
X-RAY DIFFRACTIONf_angle_d1.08136602
X-RAY DIFFRACTIONf_dihedral_angle_d31.5374126
X-RAY DIFFRACTIONf_chiral_restr0.074054
X-RAY DIFFRACTIONf_plane_restr0.0084746
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A9824X-RAY DIFFRACTION9.809TORSIONAL
12B9824X-RAY DIFFRACTION9.809TORSIONAL
13C9824X-RAY DIFFRACTION9.809TORSIONAL
14D9824X-RAY DIFFRACTION9.809TORSIONAL
15E9824X-RAY DIFFRACTION9.809TORSIONAL
16F9824X-RAY DIFFRACTION9.809TORSIONAL
17G9824X-RAY DIFFRACTION9.809TORSIONAL
18H9824X-RAY DIFFRACTION9.809TORSIONAL
21I528X-RAY DIFFRACTION9.809TORSIONAL
22J528X-RAY DIFFRACTION9.809TORSIONAL
23K528X-RAY DIFFRACTION9.809TORSIONAL
24L528X-RAY DIFFRACTION9.809TORSIONAL
25M528X-RAY DIFFRACTION9.809TORSIONAL
26N528X-RAY DIFFRACTION9.809TORSIONAL
27O528X-RAY DIFFRACTION9.809TORSIONAL
28P528X-RAY DIFFRACTION9.809TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
5.99-6.210.5111390.50112426256594
6.21-6.460.44941190.45222475259495
6.46-6.750.55321200.44322500262095
6.75-7.10.48331500.44052433258394
7.1-7.550.65161380.48822481261995
7.55-8.130.50871220.42382482260495
8.13-8.940.47981410.41182472261395
8.94-10.230.44361310.35352464259595
10.23-12.840.32141290.30692467259695
12.85-49.170.39581470.34422492263994
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.15380.54840.92721.73161.86952.18560.58810.2074-0.9518-0.6091.2850.2011-1.12140.63371.43790.3934-0.79571.0250.96640.64954.2936-112.7383-111.043635.3154
20.1023-0.1202-0.15520.45320.13570.2222-0.6450.1741-0.7911-0.0854-0.23110.63910.4002-0.4946-0.37190.6845-1.08531.290.20020.91983.5462-114.2056-92.198333.0021
30.29670.088-0.63040.22730.2184-0.26710.03790.4466-0.01450.18690.50690.28941.2306-0.0869-0.3916-0.2223-1.23560.2993-1.0307-1.16172.8487-92.4277-97.413726.798
40.57910.46840.21460.64250.18490.146-0.1499-0.41730.3399-0.0606-0.33310.24560.2777-0.5702-0.00980.851-0.74860.3951.6553-0.94982.7269-35.0258-87.968518.6216
50.15730.0449-0.4060.38450.02740.76080.732-0.5620.20080.46920.2861-0.1518-0.4403-0.18531.17561.1390.21731.4445-0.5867-1.41493.4702-51.953-116.5526-1.1358
60.54650.4561-0.08340.5894-0.0880.73160.58860.44470.6229-0.5059-0.15850.47-0.6168-0.4025-0.0197-0.59030.47340.01321.01450.14582.4577-36.8958-111.848-10.5691
70.81930.33840.12780.33450.3356-0.16380.2925-0.4433-0.12540.0368-0.1470.7030.6501-0.39322.08580.3614-0.3596-0.39881.6005-1.86212.2814-36.061-129.8621-7.8223
80.85420.9644-0.38980.96530.05710.2287-0.4080.0233-0.7972-0.1540.4878-0.2432-0.9231-1.03792.9062-0.4084-1.8471-1.0552-1.1018-0.61554.6693-4.0044-163.8826-19.8822
90.62740.2062-0.02030.03230.0560.35820.31890.19230.23890.41950.4249-0.12140.40960.17330.54320.38750.4702-0.49780.65170.96554.1288-134.937-207.9218-7.2712
100.4292-0.05690.35340.3143-0.04430.57670.5795-0.3176-0.13590.28740.2634-0.4280.6189-0.17870.76410.65361.302-0.48590.48280.98944.2681-126.3282-222.17744.0213
110.11740.2183-0.08450.487-0.27110.15130.48530.3504-0.5564-0.8229-0.8559-0.42220.77950.7025-0.00030.18160.8667-0.14421.47830.28183.3456-145.6675-220.7004-10.4415
120.18180.24490.04890.1441-0.070.01710.156-0.1165-0.2593-0.033-0.1805-0.1907-0.55790.1247-0.1809-0.5617-0.1018-0.01392.09451.4192.4985-139.2391-213.4799-3.3891
130.06170.0937-0.13990.115-0.14910.10760.2518-0.4279-0.36180.3626-0.05280.12460.36340.0506-0.3701-0.23190.02612.53980.83011.20852.5969-160.9577-189.7889-16.1923
140.2120.0906-0.15321.0722-0.30050.33960.1475-0.15520.78120.01930.627-0.07120.54850.74951.15751.44290.21161.19110.03810.47032.6015-182.9416-159.9164-20.7015
151.26890.28561.03730.7477-0.0040.93631.14210.163-0.5861-0.49310.6933-0.29120.0339-0.28410.96060.89140.57181.64890.11680.18324.3639-140.9201-128.7041-14.0015
160.1923-0.17770.03320.2633-0.20640.2639-0.1829-0.75970.32430.52-0.3805-0.7133-1.1096-0.46460.2301-0.46580.1424-0.76322.0083-0.5673.1022-156.1787-122.2206-7.8108
170.2558-0.19350.04420.38280.11660.39510.5020.4279-0.24430.2996-0.5806-0.20170.66010.2309-0.43030.0995-0.1389-0.5531.52391.03663.7217-167.2303-146.7379-5.0034
180.8861-0.00460.0430.2532-0.1118-0.1121-0.11210.4962-0.28390.0559-0.01060.1455-0.16850.8628-1.02851.28432.1076-0.72670.20930.18152.6438-199.5013-182.56471.8012
190.1898-0.32380.21360.3941-0.32970.38330.2449-0.0096-0.0565-0.0593-0.0994-0.54760.1158-0.64050.34730.25760.9801-0.9840.33861.09164.2854-121.309-236.9208-53.914
200.488-0.2304-0.28120.51380.08310.16560.0469-0.0004-0.06870.18850.4214-0.42040.56870.5050.17320.99770.6755-0.56092.1891-0.18822.857-123.969-245.2022-55.4823
210.46920.15090.33460.29050.51920.12230.1472-1.09450.3677-0.12110.7907-0.3227-1.00920.7862-0.5897-0.80791.3374-0.0096-0.5465-0.95553.3031-104.4389-252.2106-45.6281
220.0002-0.0016-0.01520.26980.17510.113-0.51950.1586-0.11590.276-0.71190.4992-0.1994-0.8982-0.47520.83370.4511-0.1493-0.4309-1.55223.7649-45.2172-256.5863-37.8082
230.6811-0.35740.08170.7729-0.15860.7620.77890.0313-0.2176-0.34910.68010.35540.18990.35951.3883-0.7831-0.95221.7040.8909-0.4134.0848-76.215-119.9722-56.6039
242.7254-0.18960.16810.3649-0.0268-0.00210.17880.33350.05930.0760.11310.3898-0.01830.4362-0.70461.05661.19760.13432.18031.24513.3978-78.6077-111.8135-51.2258
250.42510.39440.17820.44520.14480.2666-0.05270.3121-0.57130.001-0.1148-0.16280.05320.1045-0.34330.02450.1298-0.17490.7359-0.87112.606-79.6172-97.8656-50.2152
260.0908-0.7112-0.44852.8827-0.19631.04330.95250.4586-0.2940.02250.91571.98010.2147-1.66278.86820.10520.7033-0.09030.33151.17393.1465-114.584-106.1345-42.9008
271.05170.34760.11320.2293-0.07590.54330.48170.0373-0.0950.1587-0.11920.7551-0.1666-0.49621.01810.9251-0.4075-1.13320.2979-0.25024.2065-63.7155-225.5239-16.4072
280.47110.02580.08110.0959-0.12340.16140.0555-0.3092-0.36260.1787-0.31110.36220.1806-0.09940.08791.2248-0.4993-0.02331.8693-0.26222.4113-57.5181-231.4469-17.306
290.5335-0.119-0.45760.19440.52940.9828-0.2126-0.0546-0.80990.5602-1.3627-0.16140.48410.4488-1.25860.7947-0.1788-0.70250.52720.08372.6139-46.1264-231.5842-7.5464
300.0908-0.15490.05870.2070.26150.4492-0.00140.7658-0.1660.459-0.90560.445-0.1097-0.4114-0.74011.4551-1.44760.26740.688-2.24694.3789-42.6485-205.372-6.475
310.1368-0.07260.24590.31780.16550.8371-0.41310.1533-0.4012-0.3899-0.1273-0.3247-0.08380.6066-0.11172.2014-0.93651.53032.2789-0.51773.5762-25.2563-176.5809-4.0719
320.7235-0.1995-0.24270.64270.32211.7107-0.40640.3107-0.46090.1345-0.17510.01880.14940.0987-0.79081.04560.01940.72241.45410.39122.1651-6.6728-159.54942.3115
330.35310.02530.07280.25070.29040.3720.5591-0.18440.5034-0.37760.59610.05090.16420.32380.71551.3378-0.4678-0.16290.8883-0.93533.324-36.7046-180.7742-45.1472
340.21340.04160.01040.13650.02380.00820.3751-0.14760.11980.20440.03280.3584-0.33340.11020.08741.44250.0710.27941.0747-2.15082.948-26.5979-167.1884-34.7105
350.0265-0.0099-0.01310.5643-0.14280.0375-0.37870.3046-0.2398-0.2995-0.23220.1855-0.39960.3732-0.1091.46720.8154-0.84970.4744-1.06084.3043-23.8536-183.7498-45.0872
360.3537-0.06140.51730.1620.01270.5777-1.0863-0.15940.7341-0.5098-0.05850.1260.2670.0228-0.55842.3096-0.8150.50211.0306-0.56641.8664-19.1944-181.9343-50.4693
371.5219-0.17450.65580.0422-0.07320.19151.2813-1.09091.72820.33390.5229-0.4353-1.42912.22741.3556-1.96672.3709-2.7611-0.76381.06233.2007-50.4028-225.1654-56.0509
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 40 through 153 )A40 - 153
2X-RAY DIFFRACTION2chain 'A' and (resid 154 through 199 )A154 - 199
3X-RAY DIFFRACTION3chain 'A' and (resid 200 through 386 )A200 - 386
4X-RAY DIFFRACTION4chain 'A' and (resid 387 through 457 )A387 - 457
5X-RAY DIFFRACTION5chain 'B' and (resid 40 through 138 )B40 - 138
6X-RAY DIFFRACTION6chain 'B' and (resid 139 through 251 )B139 - 251
7X-RAY DIFFRACTION7chain 'B' and (resid 252 through 331 )B252 - 331
8X-RAY DIFFRACTION8chain 'B' and (resid 332 through 457 )B332 - 457
9X-RAY DIFFRACTION9chain 'C' and (resid 40 through 85 )C40 - 85
10X-RAY DIFFRACTION10chain 'C' and (resid 86 through 136 )C86 - 136
11X-RAY DIFFRACTION11chain 'C' and (resid 137 through 243 )C137 - 243
12X-RAY DIFFRACTION12chain 'C' and (resid 244 through 303 )C244 - 303
13X-RAY DIFFRACTION13chain 'C' and (resid 304 through 367 )C304 - 367
14X-RAY DIFFRACTION14chain 'C' and (resid 368 through 457 )C368 - 457
15X-RAY DIFFRACTION15chain 'E' and (resid 40 through 136 )E40 - 136
16X-RAY DIFFRACTION16chain 'E' and (resid 137 through 258 )E137 - 258
17X-RAY DIFFRACTION17chain 'E' and (resid 259 through 376 )E259 - 376
18X-RAY DIFFRACTION18chain 'E' and (resid 377 through 457 )E377 - 457
19X-RAY DIFFRACTION19chain 'D' and (resid 40 through 116 )D40 - 116
20X-RAY DIFFRACTION20chain 'D' and (resid 117 through 160 )D117 - 160
21X-RAY DIFFRACTION21chain 'D' and (resid 161 through 376 )D161 - 376
22X-RAY DIFFRACTION22chain 'D' and (resid 377 through 457 )D377 - 457
23X-RAY DIFFRACTION23chain 'F' and (resid 40 through 138 )F40 - 138
24X-RAY DIFFRACTION24chain 'F' and (resid 139 through 163 )F139 - 163
25X-RAY DIFFRACTION25chain 'F' and (resid 164 through 204 )F164 - 204
26X-RAY DIFFRACTION26chain 'F' and (resid 205 through 457 )F205 - 457
27X-RAY DIFFRACTION27chain 'G' and (resid 40 through 116 )G40 - 116
28X-RAY DIFFRACTION28chain 'G' and (resid 117 through 159 )G117 - 159
29X-RAY DIFFRACTION29chain 'G' and (resid 160 through 255 )G160 - 255
30X-RAY DIFFRACTION30chain 'G' and (resid 271 through 367 )G271 - 367
31X-RAY DIFFRACTION31chain 'G' and (resid 368 through 406 )G368 - 406
32X-RAY DIFFRACTION32chain 'G' and (resid 407 through 457 )G407 - 457
33X-RAY DIFFRACTION33chain 'H' and (resid 40 through 85 )H40 - 85
34X-RAY DIFFRACTION34chain 'H' and (resid 86 through 138 )H86 - 138
35X-RAY DIFFRACTION35chain 'H' and (resid 139 through 182 )H139 - 182
36X-RAY DIFFRACTION36chain 'H' and (resid 183 through 279 )H183 - 279
37X-RAY DIFFRACTION37chain 'H' and (resid 280 through 457 )H280 - 457

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