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Open data
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Basic information
Entry | Database: PDB / ID: 7kyz | ||||||
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Title | Solution structures of full-length K-RAS bound to GDP | ||||||
![]() | Isoform 2B of GTPase KRas | ||||||
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Function / homology | ![]() forebrain astrocyte development / negative regulation of epithelial cell differentiation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Sharma, A.K. / Maciag, A.E. | ||||||
![]() | ![]() Title: Solution structures of full-length K-RAS bound to GDP Authors: Sharma, A.K. / Maciag, A.E. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 980.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 21459.605 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Details: Samples were freshly prepared in NMR buffer pH 6.5. NMR Data was collected with 320 uL sample volume at 298K. Sample apparently held the concentration during the week long data acquisition ...Details: Samples were freshly prepared in NMR buffer pH 6.5. NMR Data was collected with 320 uL sample volume at 298K. Sample apparently held the concentration during the week long data acquisition with one sample. Two 13C15N labeled NMR samples and one 15N labeled NMR sample was used for all data collection. Ionic strength: 0.15 M / Label: conditions_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 4 Details: structures are based on 2140 distance restraints, 138 hydrogen bond restraints as deduced from secondary structure and the tertiary fold detremined using refinement process, and 238 dihedral angle restraints | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |