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- PDB-7kug: Fe-S cluster-bound transcription activator WhiB7 in complex with ... -

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Basic information

Entry
Database: PDB / ID: 7kug
TitleFe-S cluster-bound transcription activator WhiB7 in complex with the SigmaAr4-RNAP Beta flap tip chimera
Components
  • Probable transcriptional regulator WhiB7
  • RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera
KeywordsTRANSCRIPTION / Redox-sensitive / Iron-sulfur cluster / activator
Function / homology
Function and homology information


dinitrosyl-iron complex binding / protein-disulfide reductase (NAD(P)H) activity / response to fatty acid / sigma factor activity / DNA-directed RNA polymerase complex / cell redox homeostasis / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase ...dinitrosyl-iron complex binding / protein-disulfide reductase (NAD(P)H) activity / response to fatty acid / sigma factor activity / DNA-directed RNA polymerase complex / cell redox homeostasis / DNA-templated transcription initiation / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / 4 iron, 4 sulfur cluster binding / response to antibiotic / negative regulation of DNA-templated transcription / DNA-templated transcription / DNA binding / metal ion binding / cytoplasm
Similarity search - Function
AT hook motif / Transcription factor WhiB / WhiB-like iron-sulfur binding domain / Transcription factor WhiB / 4Fe-4S WhiB-like (Wbl)-type iron-sulfur binding domain profile. / AT hook, DNA-binding motif / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / RNA polymerase sigma-70 region 1.2 ...AT hook motif / Transcription factor WhiB / WhiB-like iron-sulfur binding domain / Transcription factor WhiB / 4Fe-4S WhiB-like (Wbl)-type iron-sulfur binding domain profile. / AT hook, DNA-binding motif / Sigma-70 factors family signature 1. / RNA polymerase sigma factor RpoD, C-terminal / RNA polymerase sigma factor RpoD / RNA polymerase sigma-70 region 1.2 / Sigma-70 factor, region 1.2 / RNA polymerase sigma-70 region 3 / Sigma-70 region 3 / Sigma-70 factors family signature 2. / RNA polymerase sigma-70 / RNA polymerase sigma-70 region 4 / Sigma-70, region 4 / RNA polymerase sigma-70 region 2 / RNA polymerase sigma-70 like domain / Sigma-70 region 2 / RNA polymerase sigma factor, region 2 / RNA polymerase sigma factor, region 3/4-like / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / DNA-directed RNA polymerase beta subunit, bacterial-type / RNA polymerase Rpb2, domain 2 superfamily / RNA polymerase, beta subunit, protrusion / RNA polymerase beta subunit / RNA polymerase Rpb2, domain 2 / RNA polymerase Rpb2, domain 2 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / Winged helix-like DNA-binding domain superfamily
Similarity search - Domain/homology
IRON/SULFUR CLUSTER / RNA polymerase sigma factor SigA / DNA-directed RNA polymerase subunit beta / Probable transcriptional regulator WhiB7
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.55 Å
AuthorsWan, T. / Horova, M. / Beltran, D.G. / Li, S.R. / Zhang, L.M.
Funding support United States, 4items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States)2562300224001 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM138157-01 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P20 GM113126 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P30 GM103335 United States
CitationJournal: Mol.Cell / Year: 2021
Title: Structural insights into the functional divergence of WhiB-like proteins in Mycobacterium tuberculosis.
Authors: Wan, T. / Horova, M. / Beltran, D.G. / Li, S. / Wong, H.X. / Zhang, L.M.
History
DepositionNov 24, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 30, 2021Provider: repository / Type: Initial release
Revision 1.1Oct 6, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.pdbx_database_id_PubMed / _citation.title / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Probable transcriptional regulator WhiB7
B: RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera
C: Probable transcriptional regulator WhiB7
D: RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)43,8636
Polymers43,1604
Non-polymers7032
Water5,999333
1
A: Probable transcriptional regulator WhiB7
B: RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,9313
Polymers21,5802
Non-polymers3521
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1600 Å2
ΔGint-31 kcal/mol
Surface area8900 Å2
MethodPISA
2
C: Probable transcriptional regulator WhiB7
D: RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,9313
Polymers21,5802
Non-polymers3521
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1590 Å2
ΔGint-31 kcal/mol
Surface area9040 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.586, 61.028, 210.225
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Components on special symmetry positions
IDModelComponents
11C-267-

HOH

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Components

#1: Protein Probable transcriptional regulator WhiB7


Mass: 8835.774 Da / Num. of mol.: 2 / Mutation: L27M, L42M
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (bacteria)
Strain: ATCC 25618 / H37Rv / Gene: whiB7, Rv3197A, Rv3197.1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(Gold) / References: UniProt: Q6MX01
#2: Protein RNA polymerase sigma factor, DNA-directed RNA polymerase subunit beta chimera / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 12744.025 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis (bacteria)
Gene: sigA, ERS007670_01286, ERS007681_03833, ERS007688_01862
Production host: Escherichia coli (E. coli) / Strain (production host): BL21(Gold)
References: UniProt: A0A654TMB9, UniProt: A1KGE7, DNA-directed RNA polymerase
#3: Chemical ChemComp-SF4 / IRON/SULFUR CLUSTER / Iron–sulfur cluster


Mass: 351.640 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Fe4S4 / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 333 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.04 Å3/Da / Density % sol: 39.7 % / Description: Thin large plates
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 0.1 M Tris pH 8.0, 10-15% PEG8000, 2-5% PGA

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRL / Beamline: BL12-2 / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 21, 2020
Details: Flat Si Rh coated M0, Kirkpatrick-Baez flat bent Si M1 & M2
RadiationMonochromator: Liquid nitrogen-cooled double crystal Si(111)
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 1.55→50 Å / Num. obs: 51356 / % possible obs: 99.3 % / Redundancy: 52.1 % / Biso Wilson estimate: 21.65 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.115 / Net I/σ(I): 40
Reflection shellResolution: 1.55→1.61 Å / Redundancy: 46.1 % / Rmerge(I) obs: 1.37 / Num. unique obs: 4871 / CC1/2: 0.805 / % possible all: 99.7

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
XDSdata reduction
SCALAdata scaling
AutoSolphasing
RefinementMethod to determine structure: SAD / Resolution: 1.55→40.69 Å / SU ML: 0.1616 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 21.249
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflectionSelection details
Rfree0.2 2516 4.91 %Random selection
Rwork0.1842 48725 --
obs0.1849 51241 99.26 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 32.25 Å2
Refinement stepCycle: LAST / Resolution: 1.55→40.69 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2468 0 16 334 2818
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01442585
X-RAY DIFFRACTIONf_angle_d1.56793520
X-RAY DIFFRACTIONf_chiral_restr0.1201393
X-RAY DIFFRACTIONf_plane_restr0.0064457
X-RAY DIFFRACTIONf_dihedral_angle_d21.2955984
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.55-1.580.35371310.3092491X-RAY DIFFRACTION91.94
1.58-1.610.29851460.27752662X-RAY DIFFRACTION99.4
1.61-1.650.25781440.24792706X-RAY DIFFRACTION99.89
1.65-1.680.27411430.23112656X-RAY DIFFRACTION99.89
1.68-1.730.23381350.21452684X-RAY DIFFRACTION99.82
1.73-1.770.19091390.20932701X-RAY DIFFRACTION99.65
1.77-1.820.21771440.2142682X-RAY DIFFRACTION99.72
1.82-1.880.20611320.19922690X-RAY DIFFRACTION99.72
1.88-1.950.22091270.20142747X-RAY DIFFRACTION99.62
1.95-2.030.21641600.19082682X-RAY DIFFRACTION99.68
2.03-2.120.22841340.18892682X-RAY DIFFRACTION99.51
2.12-2.230.18661420.1832680X-RAY DIFFRACTION99.16
2.23-2.370.20591480.18112730X-RAY DIFFRACTION99.65
2.37-2.560.19461390.18392744X-RAY DIFFRACTION99.93
2.56-2.810.1931390.1852713X-RAY DIFFRACTION99.69
2.81-3.220.16481310.18182774X-RAY DIFFRACTION99.93
3.22-4.060.19061360.16412801X-RAY DIFFRACTION99.9
4.06-40.690.18881460.16542900X-RAY DIFFRACTION99.64
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.009243073867710.0304014350074-0.004902727333150.02151886803090.03739566296-0.0089480584814-0.0286562079952-0.03885233473770.3095494388220.0224600996625-0.02452631127460.241778384071-0.5761908888780.05300607443477.1925786128E-60.253593727610.01848462619530.01053266826920.170487759542-0.002326089317720.182578152958-2.51421372645-19.8239743234-1.91227458088
20.08771178688010.2388519113190.01440141768970.310096684872-0.2045864154270.3706130358110.0196992698853-0.0230953128035-0.02868345212230.102742668542-0.0136951031873-0.0188332801998-0.013483590940.0957705586912-1.00201508417E-70.13964362589-0.0145235694919-0.002323056837920.1784855311780.02081532153020.1340250530163.08452604536-29.0890002298-5.89702709655
30.08584439142890.03609608755990.092289933712-0.01235080810560.003060198341310.0582789595531-0.03299161879240.08374915355110.000926056155137-0.09967446690160.01406000265050.0343857217935-0.0943780110575-0.2921869563415.67064864365E-70.150305167894-0.0151759149798-0.04932100859090.1837089206860.02965354822570.170403618768-7.43154485619-23.9399522533-15.2620825345
4-0.0510449512159-0.006365825925740.0371524425946-0.05183383754650.05167190335830.0433872837536-0.0865975728855-0.0160557756475-0.119383535806-0.1133331936940.140814709545-0.00114625259905-0.112297713556-0.0144334984294-9.55751543751E-70.118387952041-0.03871615886720.01090287398720.148280478160.006137016942630.1082277864991.55014386557-27.897518988-16.199876002
50.0813967587746-0.09685779328150.07578661595210.0284066638410.05974897625240.09427343310020.1121857438310.522404380716-0.614980557189-0.641153234707-0.123400514262-0.2981679380050.1573373304770.0345670056057-1.52516000215E-60.219699305193-0.0601023331114-0.01053637914870.183228026309-0.01596830280350.3018962249950.158207406023-34.8595609384-20.1662224907
60.0470773523925-0.0668152958239-0.0750916106330.05616546377740.009194717455230.234946656911-0.120900214172-0.1287989108240.2761581158980.1275374715390.203257055321-0.10711954988-0.02725509375790.0269411993428-8.70968419699E-80.235293915313-0.07278734568460.0006844553514950.222881980781-0.01885245449910.2652330277319.26925979773-4.89073415707-8.86842809949
70.1570989539090.143765082096-0.07519378385920.1592125559740.1302575486490.1814605876820.0264195334705-0.03382418742860.07966843261640.0076068574148-0.07986908999920.00721840155510.1555670383920.09201911961471.00535363655E-60.164487406927-0.0562060917548-0.01125734997870.213243090770.009578109984670.18264227207816.3586662724-13.6914887359-17.0487440897
80.1685196847550.0564886214818-0.1973293981020.21226164699-0.1266434216040.0264611688382-0.08804157386450.0441911740120.0398491422068-0.3484423373860.170764299738-0.0322716602743-0.08947363814990.1286835884457.59900832889E-80.252313355895-0.05954306371510.01223147423320.1781926840020.003176113642640.19110233169120.3264302063-11.3014336432-24.8359123687
90.2568941053340.6532714670150.0407402125890.4433143666130.0854833751070.4615618475190.04012537840280.0409127063853-0.1265157448-0.04964500148060.00634963269587-0.1896186168730.1253275879670.0740642962032.24493949653E-70.176618488992-0.06979209128080.01480113295870.187005130407-0.003529464666050.17722455857613.1313994648-21.0742537511-22.2559704755
100.005877465711120.135012835348-0.05616130027180.0444332879363-0.0734257109444-0.01054936394150.2237864270510.01447201447720.05576138638620.0214967842086-0.01624057703920.331461807158-0.00459723937940.1398141166131.66957818213E-70.144415591449-0.05324982334160.03412718281840.1384283532730.006903928124170.1509923112462.76361810504-15.6753904508-10.2227173392
110.03975067453150.01500516060840.0750899070853-0.00806447186396-0.01448175766870.0554826713278-0.0210336416307-0.01597346860070.01078931886530.4143625373190.08626159147730.3342138092770.1418013552160.0618631532771-2.39812152474E-60.4209795473030.02454055878420.009129729953930.2873905833090.03453068272110.6584589106790.93316032724-4.06905686268-13.8557860262
120.103500151129-0.0898131856986-0.1397949280830.0550700210242-0.01624248659780.100531964127-0.2363976144740.6224240490460.695057372701-0.113440013560.3771667690050.835901043747-0.146620877366-0.2202040392284.14964017137E-70.229945759533-0.0550567982516-0.08698446178980.2950360397440.09332114337830.3960138139421.96755350012-7.15706275707-19.0703225676
130.002800437385510.00111622573553-0.0194920589415-0.00138138407702-0.01977797994660.01206493250320.1930155170430.356966832607-0.0754280100495-0.0102382423836-0.305759206787-0.2347157307720.1335288823090.550294923983-4.03756282927E-70.2104146880910.04410680196590.02256502445020.294563342350.022912068170.163587341689-16.477338102-28.8163957522-50.5761371191
140.09756308774550.05435195480460.01574107013690.02966545896960.05350280231250.00171034933072-0.0740053713211-0.00807409604970.0456980087982-0.290624854577-0.04709662947720.231443165278-0.1564993431520.09770258189217.52771633141E-70.193935302597-0.0129770224535-0.04290122133440.126660873421-0.001475484117480.145953198508-24.0998140289-18.851209091-46.1424157863
150.9427832745430.243858335534-0.1698635644550.2140228428230.3952540092910.2890712217130.02430686822790.0117461479481-0.0639700483539-0.0523053148612-0.05074616064440.0391259022410.118405430701-0.10269598981-6.1325994165E-70.177427569209-0.0246053355822-0.02411152948770.1465722998290.009817741289240.15081085169-24.6767946489-30.862253979-43.2231766689
160.116066724743-0.145387684082-0.1761307861650.096346410961-0.08096179211450.1123203596510.038660359351-0.121645103602-0.0118390359926-0.0530496842588-0.1088912421480.02588860681750.0180874949885-0.0390664865341.51000110203E-60.138006203308-0.03257496801-0.006982101292730.1482959230660.0009949785891650.138082554605-24.4922716114-25.3728026777-35.4205313209
170.0241722175218-0.0252062777999-0.0426893745750.0397318465418-0.004078858934040.06436880631080.179646431033-0.4156856151770.09006831529880.09589026255110.247881470990.6670987028850.350071859359-0.5079690100987.59347991709E-60.259774499926-0.092365895473-0.03088382368080.2716974562550.03758159869740.335387898978-33.0520528896-28.4987072395-33.4023608738
180.05313327549950.01039181835530.01348660147620.05113318985210.1005071242220.2059718855380.08472063931030.4656109977040.394875809628-0.227722457375-0.0567628591392-0.231875266388-0.001362090172770.087845654061.15756481906E-70.249243349945-0.08635192402210.03249508591380.203450027115-0.005804397253720.2431929241-2.86192171172-15.1722129661-43.3754661596
190.08996758972980.0969239198699-0.1067377452510.09939091734690.0848997038820.0772428657871-0.1418066674110.03476250449420.0196769296621-0.01333748465730.068699398684-0.110200758085-0.3194911733380.02675502319794.56995272474E-70.185486301058-0.0446816785351-0.01153568735020.1642971480440.01282261441090.171824797422-12.5443588254-9.07618429377-35.4219429342
200.2153735236220.0815245684174-0.007400162741780.1077319688220.148812621740.2168869662460.0693224580157-0.3315543641050.03814404296760.0661759851587-0.0568551262942-0.00521151775136-0.08540367658620.139737539923-1.94384061682E-90.175613397693-0.06085251747260.01526693204560.225396318104-0.01152578285390.203130764471-10.8946852041-4.72513511628-27.4970954869
21-0.0328522334990.159793559905-0.02756188801960.28890958914-0.001539742797810.1357435063860.148028974037-0.1055954527850.02593735251650.181809800686-0.15164705543-0.005477553168430.05990813167920.02589539030251.27655784199E-60.181055193194-0.09924178497420.02026973547320.145497681145-0.0174443346430.165751827061-19.6046925447-13.1674740682-30.247741824
220.08395246323440.09518963077540.07412922382780.05607341370440.05605315094430.0547854176985-0.04284201315860.013310597141-0.243633634798-0.09822264439310.107437362705-0.08748486673710.002141418410930.1738091755467.62801852361E-70.18828238561-0.03839107493750.01320597951850.157920448474-0.03471284591560.230009848117-10.2551001459-23.7769843238-42.4507735155
23-0.0512371828974-0.01678035672980.03905236162520.00935202424027-0.0359230576501-0.0278566995026-0.314860010176-0.0374957401516-0.2448489306590.392805599780.575285650153-0.3048044370110.2711380513590.07183382556857.71224245267E-60.388508588812-0.0756308957779-0.1014634204320.3580055044110.09419608199120.6662764603282.65662188787-24.6183297616-32.8622950172
240.134175413059-0.05516412350360.008983917127570.06592548673920.2959767136590.3855594894890.206906280961-0.85184789484-1.369379067750.591583037949-0.313139691032-0.5883354870440.05016412663620.5952562818621.97584005566E-60.252524071456-0.00946468391098-0.05250557424990.2346742855610.1859483086620.585941674045-4.27364564144-21.8999244058-33.286788853
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 16 through 21 )A16 - 21
2X-RAY DIFFRACTION2chain 'A' and (resid 22 through 48 )A22 - 48
3X-RAY DIFFRACTION3chain 'A' and (resid 49 through 59 )A49 - 59
4X-RAY DIFFRACTION4chain 'A' and (resid 60 through 72 )A60 - 72
5X-RAY DIFFRACTION5chain 'A' and (resid 73 through 79 )A73 - 79
6X-RAY DIFFRACTION6chain 'B' and (resid 452 through 466 )B452 - 466
7X-RAY DIFFRACTION7chain 'B' and (resid 467 through 479 )B467 - 479
8X-RAY DIFFRACTION8chain 'B' and (resid 480 through 499 )B480 - 499
9X-RAY DIFFRACTION9chain 'B' and (resid 500 through 513 )B500 - 513
10X-RAY DIFFRACTION10chain 'B' and (resid 514 through 523 )B514 - 523
11X-RAY DIFFRACTION11chain 'B' and (resid 524 through 535 )B524 - 535
12X-RAY DIFFRACTION12chain 'B' and (resid 536 through 549 )B536 - 549
13X-RAY DIFFRACTION13chain 'C' and (resid 16 through 21 )C16 - 21
14X-RAY DIFFRACTION14chain 'C' and (resid 22 through 32 )C22 - 32
15X-RAY DIFFRACTION15chain 'C' and (resid 33 through 58 )C33 - 58
16X-RAY DIFFRACTION16chain 'C' and (resid 59 through 73 )C59 - 73
17X-RAY DIFFRACTION17chain 'C' and (resid 74 through 79 )C74 - 79
18X-RAY DIFFRACTION18chain 'D' and (resid 452 through 466 )D452 - 466
19X-RAY DIFFRACTION19chain 'D' and (resid 467 through 479 )D467 - 479
20X-RAY DIFFRACTION20chain 'D' and (resid 480 through 499 )D480 - 499
21X-RAY DIFFRACTION21chain 'D' and (resid 500 through 513 )D500 - 513
22X-RAY DIFFRACTION22chain 'D' and (resid 514 through 526 )D514 - 526
23X-RAY DIFFRACTION23chain 'D' and (resid 527 through 535 )D527 - 535
24X-RAY DIFFRACTION24chain 'D' and (resid 536 through 547 )D536 - 547

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