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Yorodumi- PDB-7drv: Structural basis of SARS-CoV-2-closely-related bat coronavirus Ra... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7drv | ||||||
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Title | Structural basis of SARS-CoV-2-closely-related bat coronavirus RaTG13 to hACE2 | ||||||
Components |
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Keywords | HYDROLASE/VIRAL PROTEIN / RaTG13 / RBD / SARS-CoV-2 / ACE2 / VIRAL PROTEIN / HYDROLASE-VIRAL PROTEIN complex | ||||||
Function / homology | Function and homology information positive regulation of amino acid transport / angiotensin-converting enzyme 2 / positive regulation of L-proline import across plasma membrane / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / angiotensin-mediated drinking behavior / tryptophan transport / positive regulation of gap junction assembly / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / regulation of cardiac conduction ...positive regulation of amino acid transport / angiotensin-converting enzyme 2 / positive regulation of L-proline import across plasma membrane / Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases / angiotensin-mediated drinking behavior / tryptophan transport / positive regulation of gap junction assembly / regulation of systemic arterial blood pressure by renin-angiotensin / regulation of vasoconstriction / regulation of cardiac conduction / peptidyl-dipeptidase activity / angiotensin maturation / maternal process involved in female pregnancy / Metabolism of Angiotensinogen to Angiotensins / metallocarboxypeptidase activity / Attachment and Entry / carboxypeptidase activity / negative regulation of signaling receptor activity / positive regulation of cardiac muscle contraction / regulation of cytokine production / viral life cycle / blood vessel diameter maintenance / brush border membrane / regulation of transmembrane transporter activity / negative regulation of smooth muscle cell proliferation / endocytosis involved in viral entry into host cell / cilium / negative regulation of ERK1 and ERK2 cascade / endocytic vesicle membrane / metallopeptidase activity / positive regulation of reactive oxygen species metabolic process / virus receptor activity / regulation of cell population proliferation / regulation of inflammatory response / endopeptidase activity / Induction of Cell-Cell Fusion / Potential therapeutics for SARS / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / entry receptor-mediated virion attachment to host cell / receptor-mediated endocytosis of virus by host cell / Attachment and Entry / membrane fusion / receptor-mediated virion attachment to host cell / symbiont entry into host cell / membrane raft / apical plasma membrane / fusion of virus membrane with host plasma membrane / endoplasmic reticulum lumen / fusion of virus membrane with host endosome membrane / viral envelope / host cell plasma membrane / virion membrane / cell surface / extracellular space / extracellular exosome / zinc ion binding / extracellular region / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Bat coronavirus RaTG13 | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.09 Å | ||||||
Authors | Liu, K.F. / Pan, X.Q. / Li, L.J. / Feng, Y. / Meng, Y.M. / Zhang, Y.F. / Wu, L.L. / Chen, Q. / Zheng, A.Q. / Song, C.L. ...Liu, K.F. / Pan, X.Q. / Li, L.J. / Feng, Y. / Meng, Y.M. / Zhang, Y.F. / Wu, L.L. / Chen, Q. / Zheng, A.Q. / Song, C.L. / Jia, Y.F. / Niu, S. / Qiao, C.P. / Zhao, X. / Ma, D.L. / Ma, X.P. / Tan, S.G. / Qi, J.X. / Gao, G.F. / Wang, Q.H. | ||||||
Citation | Journal: Cell / Year: 2021 Title: Binding and molecular basis of the bat coronavirus RaTG13 virus to ACE2 in humans and other species. Authors: Liu, K. / Pan, X. / Li, L. / Yu, F. / Zheng, A. / Du, P. / Han, P. / Meng, Y. / Zhang, Y. / Wu, L. / Chen, Q. / Song, C. / Jia, Y. / Niu, S. / Lu, D. / Qiao, C. / Chen, Z. / Ma, D. / Ma, X. ...Authors: Liu, K. / Pan, X. / Li, L. / Yu, F. / Zheng, A. / Du, P. / Han, P. / Meng, Y. / Zhang, Y. / Wu, L. / Chen, Q. / Song, C. / Jia, Y. / Niu, S. / Lu, D. / Qiao, C. / Chen, Z. / Ma, D. / Ma, X. / Tan, S. / Zhao, X. / Qi, J. / Gao, G.F. / Wang, Q. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7drv.cif.gz | 738.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7drv.ent.gz | 550.5 KB | Display | PDB format |
PDBx/mmJSON format | 7drv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dr/7drv ftp://data.pdbj.org/pub/pdb/validation_reports/dr/7drv | HTTPS FTP |
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-Related structure data
Related structure data | 6lzgS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 69038.578 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ACE2, UNQ868/PRO1885 Production host: Insect cell expression vector pTIE1 (others) References: UniProt: Q9BYF1, angiotensin-converting enzyme 2, Hydrolases; Acting on peptide bonds (peptidases); Metallocarboxypeptidases #2: Protein | Mass: 25150.430 Da / Num. of mol.: 2 / Fragment: RBD Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bat coronavirus RaTG13 Production host: Insect cell expression vector pTIE1 (others) References: UniProt: A0A6B9WHD3 #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Sugar | ChemComp-NAG / #5: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.93 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 0.1 M succinic acid pH 7.0, 0.1 M bicine pH 8.5, 30% v/v polyethylene glycol monomethyl ether 550 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97853 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 10, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97853 Å / Relative weight: 1 |
Reflection | Resolution: 3.09→48.94 Å / Num. obs: 39622 / % possible obs: 99.5 % / Redundancy: 6.8 % / Biso Wilson estimate: 94.99 Å2 / Rmerge(I) obs: 0.106 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 3.1→3.21 Å / Rmerge(I) obs: 1.31 / Num. unique obs: 3806 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6LZG Resolution: 3.09→48.94 Å / SU ML: 0.5023 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 31.5193 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 118.66 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.09→48.94 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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