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Yorodumi- PDB-7cu5: N-Glycosylation of PD-1 and glycosylation dependent binding of PD... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7cu5 | ||||||
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Title | N-Glycosylation of PD-1 and glycosylation dependent binding of PD-1 specific monoclonal antibody camrelizumab | ||||||
Components |
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Keywords | IMMUNE SYSTEM / Camrelizumab / glycosylation / PD-1. | ||||||
Function / homology | Function and homology information negative regulation of tolerance induction / regulatory T cell apoptotic process / negative regulation of immune response / B cell apoptotic process / positive regulation of T cell apoptotic process / negative regulation of B cell apoptotic process / humoral immune response / PD-1 signaling / regulation of immune response / Potential therapeutics for SARS ...negative regulation of tolerance induction / regulatory T cell apoptotic process / negative regulation of immune response / B cell apoptotic process / positive regulation of T cell apoptotic process / negative regulation of B cell apoptotic process / humoral immune response / PD-1 signaling / regulation of immune response / Potential therapeutics for SARS / adaptive immune response / external side of plasma membrane / apoptotic process / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.81 Å | ||||||
Authors | Liu, K.F. / Tan, S.G. / Jin, W.J. / Guan, J.W. / Wang, W.L. / Sun, H. / Qi, J.X. / Yan, J.H. / Chai, Y. / Wang, Z.F. ...Liu, K.F. / Tan, S.G. / Jin, W.J. / Guan, J.W. / Wang, W.L. / Sun, H. / Qi, J.X. / Yan, J.H. / Chai, Y. / Wang, Z.F. / Chu, X.D. / Gao, G.F. | ||||||
Citation | Journal: Embo Rep. / Year: 2020 Title: N-glycosylation of PD-1 promotes binding of camrelizumab. Authors: Liu, K. / Tan, S. / Jin, W. / Guan, J. / Wang, Q. / Sun, H. / Qi, J. / Yan, J. / Chai, Y. / Wang, Z. / Deng, C. / Gao, G.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7cu5.cif.gz | 162.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7cu5.ent.gz | 116.4 KB | Display | PDB format |
PDBx/mmJSON format | 7cu5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cu/7cu5 ftp://data.pdbj.org/pub/pdb/validation_reports/cu/7cu5 | HTTPS FTP |
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-Related structure data
Related structure data | 5wt9S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Antibody / Protein , 2 types, 4 molecules BAEQ
#1: Antibody | Mass: 24791.680 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) #2: Protein | Mass: 13327.914 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PDCD1, PD1 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q15116 |
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-Sugars , 4 types, 7 molecules
#3: Polysaccharide | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
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#4: Polysaccharide | Source method: isolated from a genetically manipulated source #5: Polysaccharide | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #6: Sugar | |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.14 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 0.1M cadmium chloride, 0.1M Na acetate, pH4.6, 30 %(v/v) PEG 400 a month later. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97918 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 14, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 27286 / % possible obs: 98.4 % / Redundancy: 3.4 % / Biso Wilson estimate: 34.4 Å2 / Rmerge(I) obs: 0.079 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 2.8→2.9 Å / Rmerge(I) obs: 0.692 / Num. unique obs: 2726 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5WT9 Resolution: 2.81→29.69 Å / SU ML: 0.3635 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 27.4888 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.81→29.69 Å
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Refine LS restraints |
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LS refinement shell |
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