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Open data
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Basic information
Entry | Database: PDB / ID: 7c8g | ||||||
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Title | Structure of alginate lyase AlyC3 | ||||||
![]() | Alginate lyase AlyC3 | ||||||
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Function / homology | ![]() ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Zhang, Y.Z. / Xu, F. / Chen, X.L. / Wang, P. | ||||||
![]() | ![]() Title: Structural and molecular basis for the substrate positioning mechanism of a new PL7 subfamily alginate lyase from the arctic. Authors: Xu, F. / Chen, X.L. / Sun, X.H. / Dong, F. / Li, C.Y. / Li, P.Y. / Ding, H. / Chen, Y. / Zhang, Y.Z. / Wang, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.6 KB | Display | ![]() |
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PDB format | ![]() | 98.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30199.135 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.56 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 20% (w/v) polyethylene glycol (PEG) 3350 and 0.2 M succinic acid (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: May 20, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.1→50 Å / Num. obs: 38033 / % possible obs: 99.9 % / Redundancy: 7 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 31.8 |
Reflection shell | Resolution: 2.1→2.18 Å / Rmerge(I) obs: 0.41 / Num. unique obs: 3711 |
-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 74.81 Å2 / Biso mean: 35.5263 Å2 / Biso min: 18.49 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.1→41.629 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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