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- PDB-7bv4: Crystal structure of STX17 LIR region in complex with GABARAP -

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Basic information

Entry
Database: PDB / ID: 7bv4
TitleCrystal structure of STX17 LIR region in complex with GABARAP
Components
  • Gamma-aminobutyric acid receptor-associated protein
  • Syntaxin-17
KeywordsSIGNALING PROTEIN / Autophagy / Synatxin 17 / GABARAP
Function / homology
Function and homology information


endoplasmic reticulum-Golgi intermediate compartment organization / smooth endoplasmic reticulum membrane / positive regulation of protein K48-linked ubiquitination / mitochondria-associated endoplasmic reticulum membrane contact site / vesicle fusion / vesicle docking / regulation of Rac protein signal transduction / SNARE complex / SNAP receptor activity / protein localization to phagophore assembly site ...endoplasmic reticulum-Golgi intermediate compartment organization / smooth endoplasmic reticulum membrane / positive regulation of protein K48-linked ubiquitination / mitochondria-associated endoplasmic reticulum membrane contact site / vesicle fusion / vesicle docking / regulation of Rac protein signal transduction / SNARE complex / SNAP receptor activity / protein localization to phagophore assembly site / GABA receptor binding / cellular response to nitrogen starvation / phosphatidylethanolamine binding / TBC/RABGAPs / COPII-mediated vesicle transport / microtubule associated complex / Macroautophagy / autophagosome membrane docking / beta-tubulin binding / COPII-coated ER to Golgi transport vesicle / Golgi organization / exocytosis / axoneme / smooth endoplasmic reticulum / autophagosome maturation / autophagosome membrane / autophagosome assembly / extrinsic apoptotic signaling pathway via death domain receptors / endoplasmic reticulum-Golgi intermediate compartment / autophagosome / protein targeting / endoplasmic reticulum to Golgi vesicle-mediated transport / endomembrane system / sperm midpiece / endoplasmic reticulum-Golgi intermediate compartment membrane / SNARE binding / macroautophagy / intracellular protein transport / ER to Golgi transport vesicle membrane / microtubule cytoskeleton organization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / actin cytoskeleton / protein transport / cytoplasmic vesicle / chemical synaptic transmission / microtubule binding / protein phosphatase binding / microtubule / lysosome / Golgi membrane / synapse / ubiquitin protein ligase binding / endoplasmic reticulum membrane / protein kinase binding / mitochondrion / plasma membrane / cytosol
Similarity search - Function
Syntaxin-17 / Syntaxin / Syntaxin/epimorphin, conserved site / Syntaxin / epimorphin family signature. / SNARE / Helical region found in SNAREs / t-SNARE coiled-coil homology domain profile. / Target SNARE coiled-coil homology domain / Autophagy protein Atg8 ubiquitin-like / Autophagy protein Atg8 ubiquitin like / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
DI(HYDROXYETHYL)ETHER / Gamma-aminobutyric acid receptor-associated protein / Syntaxin-17
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsLi, Y. / Pan, L.F.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31470749 China
National Natural Science Foundation of China (NSFC)21621002 China
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: Decoding three distinct states of the Syntaxin17 SNARE motif in mediating autophagosome-lysosome fusion.
Authors: Li, Y. / Cheng, X. / Li, M. / Wang, Y. / Fu, T. / Zhou, Z. / Wang, Y. / Gong, X. / Xu, X. / Liu, J. / Pan, L.
History
DepositionApr 9, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 2, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 16, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Nov 29, 2023Group: Advisory / Data collection ...Advisory / Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_unobs_or_zero_occ_atoms
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gamma-aminobutyric acid receptor-associated protein
C: Syntaxin-17
B: Gamma-aminobutyric acid receptor-associated protein
D: Syntaxin-17
E: Gamma-aminobutyric acid receptor-associated protein
F: Syntaxin-17
G: Gamma-aminobutyric acid receptor-associated protein
H: Syntaxin-17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)65,86311
Polymers65,5598
Non-polymers3043
Water2,342130
1
A: Gamma-aminobutyric acid receptor-associated protein
C: Syntaxin-17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,4823
Polymers16,3902
Non-polymers921
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1840 Å2
ΔGint-4 kcal/mol
Surface area7350 Å2
MethodPISA
2
B: Gamma-aminobutyric acid receptor-associated protein
D: Syntaxin-17


Theoretical massNumber of molelcules
Total (without water)16,3902
Polymers16,3902
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1720 Å2
ΔGint-4 kcal/mol
Surface area7070 Å2
MethodPISA
3
E: Gamma-aminobutyric acid receptor-associated protein
F: Syntaxin-17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,4963
Polymers16,3902
Non-polymers1061
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1380 Å2
ΔGint-7 kcal/mol
Surface area7680 Å2
MethodPISA
4
G: Gamma-aminobutyric acid receptor-associated protein
H: Syntaxin-17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)16,4963
Polymers16,3902
Non-polymers1061
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1420 Å2
ΔGint-7 kcal/mol
Surface area7640 Å2
MethodPISA
Unit cell
Length a, b, c (Å)42.943, 42.943, 245.600
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number76
Space group name H-MP41
Space group name HallP4w
Symmetry operation#1: x,y,z
#2: -y,x,z+1/4
#3: y,-x,z+3/4
#4: -x,-y,z+1/2

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Components

#1: Protein
Gamma-aminobutyric acid receptor-associated protein / GABA(A) receptor-associated protein / MM46


Mass: 13942.047 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GABARAP, FLC3B, HT004 / Production host: Escherichia coli (E. coli) / References: UniProt: O95166
#2: Protein/peptide
Syntaxin-17 /


Mass: 2447.604 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: STX17 / Production host: Escherichia coli (E. coli) / References: UniProt: P56962
#3: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O3
#4: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER / Diethylene glycol


Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H10O3
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 130 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.73 Å3/Da / Density % sol: 28.78 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop
Details: 0.12M Alcohols, 50% (v/v) Precipitant Mix 4, Buffer System 3 at pH 8.5 in Morpheus screen kit from Molecular Dimension

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97891 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Oct 29, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97891 Å / Relative weight: 1
ReflectionResolution: 2→40.54 Å / Num. obs: 29800 / % possible obs: 100 % / Redundancy: 12 % / Biso Wilson estimate: 22.17 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.121 / Net I/σ(I): 8.9
Reflection shellResolution: 2→2.03 Å / Rmerge(I) obs: 0.6 / Num. unique obs: 2976 / CC1/2: 0.89

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
HKL-3000data reduction
HKL-3000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5YIR

5yir


Resolution: 2→40.54 Å / SU ML: 0.2049 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 24.1316
RfactorNum. reflection% reflection
Rfree0.2251 1509 5.06 %
Rwork0.1776 --
obs0.1801 29793 99.87 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 29.12 Å2
Refinement stepCycle: LAST / Resolution: 2→40.54 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4347 0 20 130 4497
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00764510
X-RAY DIFFRACTIONf_angle_d0.88666073
X-RAY DIFFRACTIONf_chiral_restr0.0588631
X-RAY DIFFRACTIONf_plane_restr0.0057779
X-RAY DIFFRACTIONf_dihedral_angle_d29.5073590
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2-2.060.26151140.19322597X-RAY DIFFRACTION99.34
2.06-2.140.27411490.18682580X-RAY DIFFRACTION99.96
2.14-2.220.25861160.18792567X-RAY DIFFRACTION99.93
2.22-2.320.23691350.17512560X-RAY DIFFRACTION100
2.32-2.450.23291360.18462565X-RAY DIFFRACTION99.93
2.45-2.60.2551270.18752602X-RAY DIFFRACTION99.93
2.6-2.80.2671580.19762543X-RAY DIFFRACTION100
2.8-3.080.23161500.19712608X-RAY DIFFRACTION99.89
3.08-3.530.21151310.16852561X-RAY DIFFRACTION99.96
3.53-4.440.18951440.15092573X-RAY DIFFRACTION100
4.44-40.540.2041490.17882581X-RAY DIFFRACTION99.67
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.768297691352.18754294968-1.880530490823.18996028767-2.374942783248.66617595604-0.2626031285840.03423544825320.181786418650.3427250575380.1814149578350.09107631145320.04763456248810.3045272404560.1250004165870.206019758520.0264295819961-0.03179849109740.268968289682-0.01229760679990.17820100663115.21771626012.72838860036-31.5988816021
21.561423505780.204014224191-0.9523003788393.11794775257-0.6089666123242.629430121110.04012706220640.04702400735540.1568121703390.04282725625350.0432070153463-0.071586374516-0.1861907811250.0371295781345-0.09203505377010.100703871398-0.00367899573453-0.02978370514910.13027201311-0.01179894038350.09994978089969.032914032358.24199712417-46.8655852007
33.841328211721.25264304175-0.2940994191774.71376556423-0.4261181441492.63233073469-0.1317474648480.0673956698802-0.33645247832-0.01090774322590.143331497113-0.2154961296870.434775656735-0.02361139632990.03503903406760.1241325509250.012137049396-0.01515016513370.111847480126-0.03171768112060.087281083266612.275831216-0.348690601454-42.6429494076
42.229575237020.03865955751961.932927939858.5904662107-1.473725797079.09729478419-0.239018686577-0.3542072516860.6260564847350.2114698981410.3291078832-0.464808136537-1.14012186076-0.148528038126-0.1287385320780.2236089511170.08209515239340.05302816421370.346225859367-0.0553207824010.2191473844036.2269914690910.990323083-33.6755719913
53.94531581851-3.61328044273-1.152772625643.684068275890.2808381850052.418571261640.404436134592-0.3801380091620.319057864965-0.0968847715507-0.9622898206290.196360435324-0.04997276342920.1145794906990.5973414930020.248315862081-0.01156752143090.07191798351890.4875039484920.009237602418460.39314668843-5.6478956166211.8249815782-44.276460108
66.72033182808-0.0197906138809-0.1491429953597.890020133670.989234545286.95944587725-0.3263522476770.349235880211-0.0818916236791-0.1681446977050.1036640690350.05713042899070.027285527495-0.4883003880340.25476037130.1788247782920.0130009855401-0.01579181922090.2364605189480.03069407750730.0880672267766.25864777379-18.7275081844-24.4181146699
73.29078166289-4.47463609278-3.461705151237.388471115265.047792523775.815239041050.1674000776650.092722967220.262983071841-0.382414084005-0.08154621475880.0960713498741-0.428791705326-0.18397525747-0.06044860660710.134610368779-0.00235547858578-0.04074137099880.1399825831140.03280679952980.1640105250159.71206022602-12.4397739541-14.0718954055
81.4179769772-0.457857622619-0.1380593895762.87313213348-0.1986073019861.08511402858-0.063318906916-0.03626038155780.1618857888070.1089401651140.105321726532-0.130976494462-0.115275265056-0.0603849148375-0.0499036311330.1198399403860.0268123606865-0.02672022032210.1527265558970.01307228212330.097243449241513.8788733535-13.8917967836-6.9382009104
94.65267346576-0.6126468008310.7353906759395.773854638670.4644354589174.69448775879-0.08984283577850.0496723651202-0.234321924542-0.01352691534790.01984802851110.242693732850.4024540659240.1036176753140.09367204520520.0965308223759-0.01988999189170.009565462893070.1039497020.03373767129260.09833741457949.34739341114-21.6336483447-13.2345393976
107.58936914795-1.017519118986.182089760194.42818663187-0.9754462171445.04053011479-0.4179058020730.05126548285630.669755992368-0.124671464970.0869094019514-0.213065727035-1.334789534770.453714004190.1606798400460.310391214281-0.04316125902580.06506081699660.2903149256950.06530719699020.18592321037315.3127185521-10.5328276452-22.3932171914
112.9223640358-1.39319976952-3.07917011613.306528990621.881104179473.68049089774-0.3089824589380.8077136587760.2299300418690.01585462772040.722019552674-0.3064070736310.205984344009-0.207834488627-0.3084262277010.246053365821-0.05831919224610.0894163940270.4273136310430.05865382904520.50771340963426.8533132711-9.74294132999-11.4676213963
120.02512134515320.299817696160.118411435324.43082341625-1.3834760497.374827148040.0529120544411-0.5809248576570.08993536306810.273527240556-0.242387892211-0.5099075387860.4569442624560.8533049749390.08929249214230.0969436185150.03241480769620.09206741425080.557353954172-0.2222172827930.3008688941532.824451070816.52088873103-19.0380907576
134.369060240770.01167413104241.14032653384.00974921412.561685702843.05493250293-0.2846660643470.3493900830860.246201607953-0.9046932536960.6695456691270.203351514405-0.0148694579733-0.414402040021-0.06169170380860.392667942215-0.114359307043-0.114218521860.3998095410360.1449191308230.178207816034-8.2298832762511.3498144521-27.9799063109
148.67305883917-2.862952759774.714175188534.31609313245-2.142853691125.471987730770.1088133286830.276654024358-0.0066653537568-0.298545490323-0.192468141397-0.006119503179430.4409160931350.122117821795-0.02750185780840.103110954090.01035101460540.009076428384930.1764172099510.02040278166240.139834758512-6.741089938722.97781323172-12.9207870846
153.070208413570.02830786324351.766573619672.10939522899-0.2406057783613.762360376880.123215339699-0.2128116777430.04908818758230.1794848140850.1003290950270.3577249352210.175127053184-0.335003350097-0.2012268944440.1013023442260.006861305538450.01440650586990.1517562881650.007643009023240.157401999545-11.29580365895.90011061123-5.5067508411
169.783259437881.47889327109-5.758571679952.23237902306-0.219425394853.599728718540.236184846607-0.08012888579970.7012096125810.0504512455224-0.00271529249751-0.182395446238-0.507372108233-0.0381040218174-0.1984191430530.1474422220840.0164613048470.005681059019730.141855549908-0.03398026435640.184575724813-11.522907580617.6550961273-12.7890353403
173.9721622349-0.573081870905-4.547418928634.573057357022.602969668596.04428190080.2313174634790.1410193583990.684241467427-0.4838442057810.0535141170979-0.412457372294-0.6540419078050.205669948623-0.1280225883160.172135944843-0.0373714968565-0.02041430120990.1970204385180.06463644204620.182520212959-5.3710194371115.421475684-21.3100434308
180.349388762806-0.605781182161-0.4668081550413.43138852426-1.491541804816.20575756605-0.100204901581-0.1516909857060.07538180503620.3882833816390.253147359652-0.304443603344-0.02094259271670.5854900093640.01536525816220.1249787822360.0163289250922-0.006970355358590.227774806889-0.02392040210840.136569960957-0.6389176453057.18241403191-5.32622009634
196.907985474110.944309119868-1.465311219633.11099691002-4.472525979287.10445354242-0.140105400571.29318485969-1.51122802673-0.9029620092890.05235887043270.4412923868570.998958106438-0.713575214380.07224457236530.353970234076-0.0422871984515-0.1006943282020.397980458537-0.1347695692520.418242830861-12.8844400710.54124539685-19.8707269728
203.251546656082.72436211156-4.206633091345.1446788881-2.228609134799.038571027370.2051157029690.1933999449521.28769994676-0.1100058509950.2640015709710.8649989044680.55724753337-0.543689686488-0.3398575888830.335498943552-0.0688764527091-0.07419826875720.2867702751850.06767279947120.64809617573-24.63091015670.575637544302-8.91550952714
214.318645855421.58864973802-5.606696604366.760714457730.2633825002748.15569975605-0.230877425160.0119281625435-0.24938216006-0.2374334705620.06290814092540.4979866923480.468932254329-1.26074864897-0.01822118044490.133524446368-0.05391963008590.05200480236590.480317081430.1027686971230.272152516054-24.3869599188-15.0278239099-37.0305493213
225.837715287790.1730324788132.071612104475.30222504556-2.997438882414.23748215915-0.147466497262-0.4992485005110.1530222471040.9539179156930.735295060037-0.28756867553-0.3703375983120.420873552041-0.3018070506390.4157871200360.105504765853-0.08714119583860.392819363593-0.1130953410340.221203451743-13.258403038-10.1393627146-28.1005071794
233.35457683160.1962542890621.560571766663.071063442390.9640086171113.677146284270.0779901537970.078266419874-0.194895262199-0.0511875953880.0530510218104-0.2527623444740.4389449530690.153108249458-0.1354604110140.102788884050.0285041409626-0.00751710629440.131064030414-0.02860019089340.151008569576-11.4863859838-18.344355374-47.5251595443
246.388686291540.852727522251.08934207771.245670885910.9030940991377.09469977202-0.00671471051642-0.06104686618010.222266716793-0.110584838312-0.05105582908310.0013029224597-0.5554583667380.1453158848590.0197392196960.127316296466-0.003207520152430.0392034325960.0777702478420.03439785438350.18977872874-11.6471419929-5.22330581871-47.308720516
256.492203775980.179168420778-5.769681327083.49880786633-1.800723524596.054732640330.251410461359-0.1229324951190.9220842782640.5413584395170.1023907992270.25924466895-0.385089724133-0.115904741642-0.3861048509630.1502047586550.00183708497581-0.01141917655270.180075294405-0.04295710527980.206463583945-16.1973832464-6.07642151463-34.7284826027
260.254221489065-0.142565077325-0.5930167012443.489376146342.576964080836.12013866973-0.01199020133380.2178818219430.0725990706454-0.3413283215810.1701090729030.174783549769-0.0991935270808-0.247341469056-0.1134585189420.0986686302274-0.02966363876450.0007644110477090.2239961505930.0464710557460.125073618585-20.8248649306-14.2582668205-50.7441600111
274.63668696306-0.413772954031-3.582638663595.536470060775.54156867548.74937796556-0.142467601661-0.956057379538-1.203282270850.375626142586-0.165648831386-0.5714563971481.136295310690.33005600220.418284568990.3372273930040.0765928333059-0.05937142058110.2872631715950.07304127670640.367574348814-8.39579964338-20.8513933723-36.4163736162
283.84325586763-4.57618509804-1.428662388127.115607415464.503902077055.249718851710.411566833485-0.334013564068-0.639584508465-0.2317199099840.09634192525091.661248421461.17438247076-0.151021059228-0.2871276904160.3847595519060.0571717214187-0.1523856591140.372887664773-0.07403883316840.9385805922672.99867660691-20.7950251168-47.1954465022
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 24 )
2X-RAY DIFFRACTION2chain 'A' and (resid 25 through 90 )
3X-RAY DIFFRACTION3chain 'A' and (resid 91 through 112 )
4X-RAY DIFFRACTION4chain 'C' and (resid 169 through 175 )
5X-RAY DIFFRACTION5chain 'C' and (resid 176 through 183 )
6X-RAY DIFFRACTION6chain 'B' and (resid 1 through 24 )
7X-RAY DIFFRACTION7chain 'B' and (resid 25 through 47 )
8X-RAY DIFFRACTION8chain 'B' and (resid 48 through 90 )
9X-RAY DIFFRACTION9chain 'B' and (resid 91 through 112 )
10X-RAY DIFFRACTION10chain 'D' and (resid 169 through 175 )
11X-RAY DIFFRACTION11chain 'D' and (resid 176 through 183 )
12X-RAY DIFFRACTION12chain 'E' and (resid 1 through 10 )
13X-RAY DIFFRACTION13chain 'E' and (resid 11 through 24 )
14X-RAY DIFFRACTION14chain 'E' and (resid 25 through 47 )
15X-RAY DIFFRACTION15chain 'E' and (resid 48 through 90 )
16X-RAY DIFFRACTION16chain 'E' and (resid 91 through 98 )
17X-RAY DIFFRACTION17chain 'E' and (resid 99 through 104 )
18X-RAY DIFFRACTION18chain 'E' and (resid 105 through 117 )
19X-RAY DIFFRACTION19chain 'F' and (resid 170 through 175 )
20X-RAY DIFFRACTION20chain 'F' and (resid 176 through 184 )
21X-RAY DIFFRACTION21chain 'G' and (resid 1 through 10 )
22X-RAY DIFFRACTION22chain 'G' and (resid 11 through 24 )
23X-RAY DIFFRACTION23chain 'G' and (resid 25 through 79 )
24X-RAY DIFFRACTION24chain 'G' and (resid 80 through 98 )
25X-RAY DIFFRACTION25chain 'G' and (resid 99 through 104 )
26X-RAY DIFFRACTION26chain 'G' and (resid 105 through 117 )
27X-RAY DIFFRACTION27chain 'H' and (resid 170 through 175 )
28X-RAY DIFFRACTION28chain 'H' and (resid 176 through 184 )

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