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Yorodumi- PDB-6yt0: Magnesium chelatase H subunit (ChlH) E660D variant from Synechocy... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6yt0 | ||||||
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Title | Magnesium chelatase H subunit (ChlH) E660D variant from Synechocystis sp.PCC6803 | ||||||
Components | Mg-chelatase subunit ChlH | ||||||
Keywords | PHOTOSYNTHESIS / Magnesium chelatase / chlorophyll | ||||||
Function / homology | Function and homology information magnesium chelatase / magnesium chelatase activity / chlorophyll biosynthetic process / photosynthesis / ATP binding Similarity search - Function | ||||||
Biological species | Synechocystis sp. PCC 6803 substr. Kazusa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.85 Å | ||||||
Authors | Bisson, C. / Hunter, C.N. | ||||||
Funding support | 1items
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Citation | Journal: Nat.Plants / Year: 2020 Title: The active site of magnesium chelatase. Authors: Adams, N.B.P. / Bisson, C. / Brindley, A.A. / Farmer, D.A. / Davison, P.A. / Reid, J.D. / Hunter, C.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6yt0.cif.gz | 492 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6yt0.ent.gz | 390 KB | Display | PDB format |
PDBx/mmJSON format | 6yt0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yt/6yt0 ftp://data.pdbj.org/pub/pdb/validation_reports/yt/6yt0 | HTTPS FTP |
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-Related structure data
Related structure data | 6ys9C 6ysgSC 6ytjC 6ytnC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 150857.469 Da / Num. of mol.: 2 / Mutation: E660D Source method: isolated from a genetically manipulated source Details: N-terminal His-tag and linker. Source: (gene. exp.) Synechocystis sp. PCC 6803 substr. Kazusa (bacteria) Gene: chlH / Production host: Escherichia coli (E. coli) / References: UniProt: P73020 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.6 Å3/Da / Density % sol: 65.8 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 0.8-1.2 M sodium citrate and 2 mM dithiothreitol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.97626 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 29, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97626 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→54.6 Å / Num. obs: 94580 / % possible obs: 100 % / Redundancy: 5.8 % / CC1/2: 0.995 / Rpim(I) all: 0.061 / Net I/σ(I): 9.4 |
Reflection shell | Resolution: 2.85→2.9 Å / Mean I/σ(I) obs: 1.5 / Num. unique obs: 4748 / CC1/2: 0.56 / Rpim(I) all: 0.544 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: Direct refinement from 6YSG Resolution: 2.85→54.6 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.895 / SU B: 15.983 / SU ML: 0.283 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.767 / ESU R Free: 0.351 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 169.33 Å2 / Biso mean: 59.712 Å2 / Biso min: 13.91 Å2
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Refinement step | Cycle: final / Resolution: 2.85→54.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.85→2.924 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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